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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

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OfflineMr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
question about sterilizing a inoculation loop
    #21942336 - 07/14/15 02:46 PM (8 years, 6 months ago)

Hey everyone, so today is my first time using a print to agar. I have done a syringe to agar and I guess things are going fine. I have a inoculation loop that I am wondering and prob should of done this last night if it is the case when i mixed up my agar but do I need to pressure cook it. If so I was wondering if wrapping it up in foil and putting it in a jar would be okay and pressure cooking it for about 40 mins. I did not even think of this last night until I had already done my batch of agar.


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
Re: question about sterilizing a inoculation loop [Re: Mr.Top_Hat]
    #21942357 - 07/14/15 02:51 PM (8 years, 6 months ago)

Wipe down with alcohol, wait for it to dry, then flame it like you would a needle.


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OfflineMr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
Re: question about sterilizing a inoculation loop [Re: TravelAgency]
    #21942361 - 07/14/15 02:53 PM (8 years, 6 months ago)

thats great i was about to pressure sterilize it I have never used one before so trying all these new things and making sure i am as sterile as possible is getting a little overwhelming but again thanks for the answer


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
Re: question about sterilizing a inoculation loop [Re: Mr.Top_Hat]
    #21942372 - 07/14/15 02:55 PM (8 years, 6 months ago)

Absolutely! Good luck with the agar- I'm waiting for mine to arrive in the mail... On pins and needles. I have a couple fruits coming up now I want to clone (one looks like it might be an albino ecuadorian, one is just massive and fast) I hope it gets here in time but it's doubtful


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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures


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