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uspislig
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Myco/grow bag..help!
#21941023 - 07/14/15 08:17 AM (8 years, 6 months ago) |
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Hi! I have a grow bag that i inoculated (or incubated? noob here) with spores a month ago. As far as the mycelium growth goes its about 75% covered. The problem is i left for three weeks and forgot to make sure the bag was in a dark location. It got indirect sunlight for three weeks. So when i got back there were two visible mushrooms growing ( not strait up because the bag was nt straitened out). Im happy to see growth, but the mycelium has nt colonized fully yet. So my questions are:
-Should i put it in a dark location and wait for the mycelium to colonize fully? Even though there is growth happening.
-Or should i put a 12/12 light on it and just leave it and hope for the best?
-Also, if i put on the light and continue the fruiting, will the mycelium still colonize the whole bag AND produce mushrooms?
I have been reading, but people say different things. THANKS!
btw this forum is great!
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Jii
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Re: Myco/grow bag..help! [Re: uspislig]
#21941040 - 07/14/15 08:23 AM (8 years, 6 months ago) |
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If it fruits before full colonization it could be contaminated. Bacteria would be my first guess. Photo would help.
-------------------- My shroom inspired photo gallery - One of god's own prototypes. Too weird to live, too rare to die - I highly recommend www.genesisgeneration.us and Freedomainradio. Jii
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uspislig
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Re: Myco/grow bag..help! [Re: Jii]
#21941280 - 07/14/15 09:33 AM (8 years, 6 months ago) |
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The bag one month ago:

The bag now. You can see the two fruits on this two photos:

This is what is left to colonize:

It may be difficult to see on the photos but it doesnt seem like there is much pinning going on. So i think im gonna try to keep it in the dark for another week and just see what happens.
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Jii
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Re: Myco/grow bag..help! [Re: uspislig]
#21941400 - 07/14/15 10:05 AM (8 years, 6 months ago) |
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I'm sorry, but that looks like a goner. Bacteria has taken over the uncolonized parts. You can put it to fruiting conditions, but I would not expect too much. Make your own bags, try pf-tek or do a monotub. You will have much better results.
-------------------- My shroom inspired photo gallery - One of god's own prototypes. Too weird to live, too rare to die - I highly recommend www.genesisgeneration.us and Freedomainradio. Jii
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uspislig
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Re: Myco/grow bag..help! [Re: Jii]
#21942352 - 07/14/15 02:50 PM (8 years, 6 months ago) |
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hm, ok. But if i get fruits, will it be harmful to eat them? or is it just screwing up the flush?
Edited by uspislig (07/14/15 02:55 PM)
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Xenochrist
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Re: Myco/grow bag..help! [Re: uspislig]
#21942379 - 07/14/15 02:57 PM (8 years, 6 months ago) |
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It is an unwise decision to eat anything that goes south. You definitely have a contam growing in there. Go dig a small hole outside, dump that bag out into it, mix in a little horse shit, and cover it up. maybe you'll get something out of it in the end
-------------------- Ash nazg durbatuluk, ash nazg gimbatul, ash nazg thrakatuluk agh burzum-ishi krimpatul.
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uspislig
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Re: Myco/grow bag..help! [Re: Xenochrist]
#21942418 - 07/14/15 03:07 PM (8 years, 6 months ago) |
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f**k..this is depressing..
But if the mycel contiunes to colonize, will it then be false alarm?
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TravelAgency
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Re: Myco/grow bag..help! [Re: Xenochrist]
#21942478 - 07/14/15 03:23 PM (8 years, 6 months ago) |
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Quote:
Xenochrist said: It is an unwise decision to eat anything that goes south. You definitely have a contam growing in there. Go dig a small hole outside, dump that bag out into it, mix in a little horse shit, and cover it up. maybe you'll get something out of it in the end
I concur. I've gotten a fairly sizable amount from my outdoor contam pile.
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uspislig
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Quote:
TravelAgency said:
Quote:
Xenochrist said: It is an unwise decision to eat anything that goes south. You definitely have a contam growing in there. Go dig a small hole outside, dump that bag out into it, mix in a little horse shit, and cover it up. maybe you'll get something out of it in the end
I concur. I've gotten a fairly sizable amount from my outdoor contam pile.
Ok, but i dont have the option to do that..sadly. would it be possible to open the bag and remove the uncolonized parts and just put the colonized in a fruiting chamber? Cause that i have. Substrate is brf and verm btw. And if you dump it in a hole and get fruits, how is that any different from getting fruits from the bag? At least in the bag you can see that the mycel is contaminated, something you cant outdoors, so there might as well be bacteria on the subsrate?? Please share your knowledge
Edited by uspislig (07/14/15 03:48 PM)
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TravelAgency
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Re: Myco/grow bag..help! [Re: uspislig]
#21942620 - 07/14/15 04:01 PM (8 years, 6 months ago) |
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Honestly, the fruits should be fine as long as they look fine. However if you have a contam you want it as far away from your grow space as possible. If you were to fruit it you would probably destroy any chances you have at future/current attempts. If you don't have space outside, I'd say take the fruits you have, chunk the rest, and call it a learning experience. My first time to grow in years was just a few months ago and I totally messed up the inoculation techniques. Leaving me with only 4 of 20 jars making it. I learned (or relearned) from my mistakes, and so far have only lost one jar out of 25.
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uspislig
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Quote:
TravelAgency said: Honestly, the fruits should be fine as long as they look fine. However if you have a contam you want it as far away from your grow space as possible. If you were to fruit it you would probably destroy any chances you have at future/current attempts. If you don't have space outside, I'd say take the fruits you have, chunk the rest, and call it a learning experience. My first time to grow in years was just a few months ago and I totally messed up the inoculation techniques. Leaving me with only 4 of 20 jars making it. I learned (or relearned) from my mistakes, and so far have only lost one jar out of 25.
Okay. I am going to keep the bag in the dark for another week, and then see if the mycel has spread further. If it hasn't I will just throw it away. Learn by doing Thanks for the help!
But i still wonder; If you dump it in a hole and get fruits, how is that any different from getting fruits from the bag? At least in the bag you can see that the mycel is contaminated, something you cant outdoors, so there might as well be bacteria on the subsrate?? Wouldn't this also account for mushrooms in nature? I mean, you can't tell if there is any biological contam (like bacteria) under ground, and then they might have fruited early also. Just wondering.
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spacechildo
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Re: Myco/grow bag..help! [Re: uspislig]
#21942765 - 07/14/15 04:34 PM (8 years, 6 months ago) |
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what is in those grow bags?
darkness has nothing to do with early pinning, contamination or compression has.
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uspislig
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okay. I thought maybe the daylight had triggered the pinning. But then again im a newb xD The substrate is verm and brf i presume.
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uspislig
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Quote:
spacechildo said: what is in those grow bags?
darkness has nothing to do with early pinning, contamination or compression has.
compression??? specify please.
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spacechildo
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Re: Myco/grow bag..help! [Re: uspislig]
#21943127 - 07/14/15 05:49 PM (8 years, 6 months ago) |
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verm and brf are usually put in 1/2 pint jars for a reason. bags like that are bound to get compressed from their own weight and water following gravity down.
the mix looks real dark and wet, did you buy this or make it yourself?
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uspislig
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I bought the bag. It looked very dry when i got it, so i was a bit conserned. But if this is caused by compression, and not bacteria, will it be okay in the end?
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Jii
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Re: Myco/grow bag..help! [Re: uspislig]
#21946491 - 07/15/15 09:16 AM (8 years, 6 months ago) |
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I guess you can try to give it massage. Spread the mycelium all over the bag and see if it recovers. That's what I do with my rye bags and I don't see any reason why it would not work with BRF/Verm bags.
-------------------- My shroom inspired photo gallery - One of god's own prototypes. Too weird to live, too rare to die - I highly recommend www.genesisgeneration.us and Freedomainradio. Jii
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uspislig
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Re: Myco/grow bag..help! [Re: Jii]
#21947852 - 07/15/15 03:09 PM (8 years, 6 months ago) |
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Ill try that. Got nothing to lose
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uspislig
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Re: Myco/grow bag..help! [Re: uspislig]
#21995911 - 07/25/15 03:46 PM (8 years, 6 months ago) |
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The mycelium has taken over the hole bag, despite some said it looked like bacteria had taken over some of the substrate.
  
Im thinking about taking a spore print of one of the fruits inside. Any thoughts on that?
I also got some blue dots on the mycel, but it just looks like bruising to me.

Just wondering what you guys think about it. Could it still be contaminated with bacteria even though the mycel has spread?
Edited by uspislig (07/25/15 04:01 PM)
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Psilosoulful

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Re: Myco/grow bag..help! [Re: uspislig]
#21996168 - 07/25/15 04:46 PM (8 years, 6 months ago) |
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Bacterial for sure. You can try taking a spore print of that fruit in the last pic, but I'd let it mature for a little while longer.
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JacobStorm
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Re: Myco/grow bag..help! [Re: uspislig]
#21996229 - 07/25/15 05:00 PM (8 years, 6 months ago) |
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Quote:
uspislig said: Hi! I have a grow bag that i inoculated (or incubated? noob here) with spores
Inoculation is the process of injecting/introducing spores into a substrate. Incubating is the action of letting your Inoculated substrate colonize at proper temperatures
-Should i put it in a dark location and wait for the mycelium to colonize fully?
Darkness is actually not a GREAT thing for Incubating. A little Indirect light will actually help the colonization process. Although I cant recall if white or blue spectrum is better for incubation. I have my BF substrate jars incubating in the cupboard above my fridge (where the temp Is consistently 77-80 degrees) and every morning I wake up I just open the cupboard until I get off work to let a bit of indirect light In there, then close it when I get off. So far so good. :
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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uspislig
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Quote:
Psilosoulful said: Bacterial for sure. You can try taking a spore print of that fruit in the last pic, but I'd let it mature for a little while longer. 
-How can you tell there is bacteria?
-Would'nt the spore print be contaminated also?
-Will the bacteria make the fruits harmful to digest?
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Psilosoulful

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Re: Myco/grow bag..help! [Re: uspislig]
#21996674 - 07/25/15 06:41 PM (8 years, 6 months ago) |
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- It looks slimy on the surface, that indicates some bacterial presence.
-Quite possibly, swab the spore print on an agar dish. When the mycelium grows out, you'll be able to spot contams. Transfer a tiny piece of healthy grow with an exacto blade and transfer to a new dish to get a monoculture if you want. OR just drop it in a grain jar.
-No just make sure it's not growing on the actual mushroom. I personally would not eat the fruits off a contaminated substrate.
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uspislig
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Quote:
Psilosoulful said: - It looks slimy on the surface, that indicates some bacterial presence.
-Quite possibly, swab the spore print on an agar dish. When the mycelium grows out, you'll be able to spot contams. Transfer a tiny piece of healthy grow with an exacto blade and transfer to a new dish to get a monoculture if you want. OR just drop it in a grain jar.
-No just make sure it's not growing on the actual mushroom. I personally would not eat the fruits off a contaminated substrate.
Thanks! If there is bacteria present, i expect a different smell when i open the bag. Any thoughts on how it should or should not smell?
I probably won't eat them. I am mostly out for the spore print
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Psilosoulful

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Re: Myco/grow bag..help! [Re: uspislig]
#21998922 - 07/26/15 08:56 AM (8 years, 6 months ago) |
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Quote:
uspislig said:
Quote:
Psilosoulful said: - It looks slimy on the surface, that indicates some bacterial presence.
-Quite possibly, swab the spore print on an agar dish. When the mycelium grows out, you'll be able to spot contams. Transfer a tiny piece of healthy grow with an exacto blade and transfer to a new dish to get a monoculture if you want. OR just drop it in a grain jar.
-No just make sure it's not growing on the actual mushroom. I personally would not eat the fruits off a contaminated substrate.
Thanks! If there is bacteria present, i expect a different smell when i open the bag. Any thoughts on how it should or should not smell?
I probably won't eat them. I am mostly out for the spore print
It would be like a slightly sour smell. Taking a spore print is a good idea. You can germinate those on an agar dish and find clean growth if you want.
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uspislig
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I have gotten some fruits from the growbag. I am wondering what you guys think and if they look good or bad. There was some belief that there was bacteria present in the bag. I did not smell anything out of the ordinary, but i did spot a peculiar growth on top of one mushroom.
I just tossed it, but do any of you know what it is?
 I personally dont see any sign of bacteria on them, do you?
    I got two spore prints from the biggest capsâș
In the second flush there was some mold on a few caps, so i just picked out the good looking ones. This is from the second flush.
  I threw out the one on the bottom in the left pic cause it doesnt look too good and i also tossed the biggest one. It had some mold on the cap.
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