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InvisibleJacobStorm
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I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: Myco/grow bag..help! [Re: uspislig]
    #21996229 - 07/25/15 05:00 PM (8 years, 6 months ago)

Quote:

uspislig said:
Hi! I have a grow bag that i inoculated (or incubated? noob here) with spores

Inoculation is the process of injecting/introducing spores into a substrate.
Incubating is the action of letting your Inoculated substrate colonize at proper temperatures

-Should i put it in a dark location and wait for the mycelium to colonize fully?

Darkness is actually not a GREAT thing for Incubating. A little Indirect light will actually help the colonization process. Although I cant recall if white or blue spectrum is better for incubation.
I have my BF substrate jars incubating in the cupboard above my fridge (where the temp Is consistently 77-80 degrees) and every morning I wake up I just open the cupboard until I get off work to let a bit of indirect light In there, then close it when I get off. So far so good.
:




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Offlineuspislig
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Registered: 05/16/15
Posts: 40
Last seen: 2 years, 5 months
Re: Myco/grow bag..help! [Re: Psilosoulful]
    #21996364 - 07/25/15 05:28 PM (8 years, 6 months ago)

Quote:

Psilosoulful said:
Bacterial for sure. You can try taking a spore print of that fruit in the last pic, but I'd let it mature for a little while longer. :goodluck:




-How can you tell there is bacteria?

-Would'nt the spore print be contaminated also?

-Will the bacteria make the fruits harmful to digest?


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Myco/grow bag..help! [Re: uspislig]
    #21996674 - 07/25/15 06:41 PM (8 years, 6 months ago)

- It looks slimy on the surface, that indicates some bacterial presence.

-Quite possibly, swab the spore print on an agar dish. When the mycelium grows out, you'll be able to spot contams. Transfer a tiny piece of healthy grow with an exacto blade and transfer to a new dish to get a monoculture if you want. OR just drop it in a grain jar.

-No just make sure it's not growing on the actual mushroom. I personally would not eat the fruits off a contaminated substrate.


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Offlineuspislig
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Re: Myco/grow bag..help! [Re: Psilosoulful]
    #21998605 - 07/26/15 07:02 AM (8 years, 6 months ago)

Quote:

Psilosoulful said:
- It looks slimy on the surface, that indicates some bacterial presence.

-Quite possibly, swab the spore print on an agar dish. When the mycelium grows out, you'll be able to spot contams. Transfer a tiny piece of healthy grow with an exacto blade and transfer to a new dish to get a monoculture if you want. OR just drop it in a grain jar.

-No just make sure it's not growing on the actual mushroom. I personally would not eat the fruits off a contaminated substrate.




Thanks!
If there is bacteria present, i expect a different smell when i open the bag. Any thoughts on how it should or should not smell?

I probably won't eat them. I am mostly out for the spore print:grin:


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Myco/grow bag..help! [Re: uspislig]
    #21998922 - 07/26/15 08:56 AM (8 years, 6 months ago)

Quote:

uspislig said:
Quote:

Psilosoulful said:
- It looks slimy on the surface, that indicates some bacterial presence.

-Quite possibly, swab the spore print on an agar dish. When the mycelium grows out, you'll be able to spot contams. Transfer a tiny piece of healthy grow with an exacto blade and transfer to a new dish to get a monoculture if you want. OR just drop it in a grain jar.

-No just make sure it's not growing on the actual mushroom. I personally would not eat the fruits off a contaminated substrate.




Thanks!
If there is bacteria present, i expect a different smell when i open the bag. Any thoughts on how it should or should not smell?

I probably won't eat them. I am mostly out for the spore print:grin:



It would be like a slightly sour smell. Taking a spore print is a good idea. You can germinate those on an agar dish and find clean growth if you want. :thumbup:


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Offlineuspislig
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Registered: 05/16/15
Posts: 40
Last seen: 2 years, 5 months
Re: Myco/grow bag..help! [Re: Psilosoulful]
    #22087326 - 08/13/15 01:49 PM (8 years, 5 months ago)

I have gotten some fruits from the growbag. I am wondering what you guys think and if they look good or bad. There was some belief that there was bacteria present in the bag. I did not smell anything out of the ordinary, but i did spot a peculiar growth on top of one mushroom.

I just tossed it, but do any of you know what it is?

I personally dont see any sign of bacteria on them, do you?

I got two spore prints from the biggest capsâ˜ș

In the second flush there was some mold on a few caps, so i just picked out the good looking ones.
This is from the second flush.

I threw out the one on the bottom in the left pic cause it doesnt look too good and i also tossed the biggest one. It had some mold on the cap.


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