Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMush-Room
Stranger
Registered: 07/14/15
Posts: 20
Last seen: 2 years, 5 months
To incubate or not to
    #21940372 - 07/14/15 02:11 AM (8 years, 6 months ago)

Hello, fellow shroomers. This is first post, but this is not my first grow, however it will be the first time I fruit. This is sort of a multifaceted question but mainly to do with incubation. I will first describe my situation and you can add from there.

In the past I inoculated 12 pint jars and left them to their own demise. All but 1 fully colonized due to contamination. Although inoculation was successful I had to abandon the grow for personal reasons and I do not know the ET of full colonization of the mycelium Web nor do I remember the entire conditions they endured.

All that aside I've gone and inoculated 12 more jars. I first sterilized 8 jars with fairly high heat. Inoculation was a bit sloppy since I could hardly read the side of the syringe and a few jars got more than 1cc. The rest were 2 inoculation points at roughly 1/2 cc at each point.

After 10 days most of these show significant signs of growth and no contamination however one stands out from the rest. Near the top of the substrate where it meets vermiculite there is what I can only describe as slime going partially around the jar. I'm guessing it's due to a heavy dose from the syringe. This one does not show signs of growth however another just like it does.

The last 4 were sterilized at a slightly lower heat but still 15 psi and inoculated with roughly 2.1cc in total. 1 just started showing signs of growth.

I've kept my jars high up in a dark closet where indirect sunlight can still reach them. The tempatures around here have been varying greatly. Using an app on my phone i recorded temperatures ranging from 65-90+°F (higher temps recorded could be due to the battery in my phone) and relative humidity from 60-90%. Now obviously these are not ideal conditions and this being the first time I will fruit I am growing impatient which is why I'm wondering if I should incubate them.

I tried moving them to my basement but fear it may be too cold,  especially when the temperature drops at night. I removed them from the basement when an ambient temp reached around 70°F and the jars felt slightly cold to the touch.  I also noticed there isnt much info on incubation on this forum or maybe it's me. Anyways If i do end up incubating what would be an ideal temp? I've read 25-27°c is ideal for colonization. And if needed can someone please link me to a good and possibly cheap set up. Thank you in advance.


Extras: Filter Print Post Top
Invisiblearpeggiator
Surprisingly Coherent
Male User Gallery


Registered: 12/15/13
Posts: 380
Loc: Hyperspace
Re: To incubate or not to [Re: Mush-Room]
    #21940493 - 07/14/15 03:16 AM (8 years, 6 months ago)

70-80 degrees is ok for both colonization and fruiting
Humidity doesn't matter for colonization in jars.

Take a picture of the jar in question  with the slime you mentioned.


--------------------


Extras: Filter Print Post Top
Offlineshroomyaxn
Male User Gallery


Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
Re: To incubate or not to [Re: Mush-Room]
    #21942068 - 07/14/15 01:32 PM (8 years, 6 months ago)

Quote:

Mush-Room said:
I first sterilized 8 jars with fairly high heat...

The last 4 were sterilized at a slightly lower heat but still 15 psi..




Heat? No need to worry about heat when sterilizing with a PC.  Just focus on keeping the PSI at 14-15psi.  That's all.

Quote:

Mush-Room said:
I've kept my jars high up in a dark closet where indirect sunlight can still reach them. The temperatures around here have been varying greatly. Using an app on my phone i recorded temperatures ranging from 65-90+°F




The indoor closet temps were at 90+°F? That is too hot for colonizing temps. 
You may be okay but temperatures in the mid to upper 80's°F is pushing it. 

Quote:

Mush-Room said:
I tried moving them to my basement but fear it may be too cold,  especially when the temperature drops at night. I removed them from the basement when an ambient temp reached around 70°F and the jars felt slightly cold to the touch.



70°F is just fine, much more ideal than 85-90°F.


Extras: Filter Print Post Top
OfflineMush-Room
Stranger
Registered: 07/14/15
Posts: 20
Last seen: 2 years, 5 months
Re: To incubate or not to [Re: shroomyaxn]
    #21942949 - 07/14/15 05:08 PM (8 years, 6 months ago)

This is the jar I was talking about

This next one is like the previous but actually started to colonize


Extras: Filter Print Post Top
OfflineDarkhome
•Shaman•N•Training•
Male User Gallery


Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: To incubate or not to [Re: Mush-Room]
    #21944511 - 07/14/15 11:02 PM (8 years, 6 months ago)

If you are comfortable... Your jars will be... Regular room temperature is best... Buy a 99 cent thermometer at Wally World or wherever... Higher temps cause bacterial growth...Try to keep the room below 80 degrees Fahrenheit because the jars will generate heat as they grow as well.:goodluck:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Extras: Filter Print Post Top
Invisiblearpeggiator
Surprisingly Coherent
Male User Gallery


Registered: 12/15/13
Posts: 380
Loc: Hyperspace
Re: To incubate or not to [Re: Mush-Room]
    #21944546 - 07/14/15 11:11 PM (8 years, 6 months ago)

Quote:

Mush-Room said:
This is the jar I was talking about

This next one is like the previous but actually started to colonize





Could be bacterial, I can't tell very well wait for more experienced people :thumbup:

Remove the foil on all of your jars, the vermiculite barrier will protect against contams.
Foil will restrict GE, slowing growth.


--------------------


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: To incubate or not to [Re: shroomyaxn]
    #21944802 - 07/15/15 12:24 AM (8 years, 6 months ago)

Quote:

shroomyaxn said:
Quote:

Mush-Room said:
I first sterilized 8 jars with fairly high heat...

The last 4 were sterilized at a slightly lower heat but still 15 psi..




Heat? No need to worry about heat when sterilizing with a PC.  Just focus on keeping the PSI at 14-15psi.  That's all.

Quote:

Mush-Room said:
I've kept my jars high up in a dark closet where indirect sunlight can still reach them. The temperatures around here have been varying greatly. Using an app on my phone i recorded temperatures ranging from 65-90+°F





The indoor closet temps were at 90+°F? That is too hot for colonizing temps. 
You may be okay but temperatures in the mid to upper 80's°F is pushing it. 

Quote:

Mush-Room said:
I tried moving them to my basement but fear it may be too cold,  especially when the temperature drops at night. I removed them from the basement when an ambient temp reached around 70°F and the jars felt slightly cold to the touch.



70°F is just fine, much more ideal than 85-90°F.




Yeah listen to this guy. My house is at 65F and colonizing beautifully. The higher the temp you go, the easier it is for contams. Also, although the sweet spot for myc is a bit higher than what my temps are at, that sweet spot is only a few degrees under the sweet spot for trich, cobweb, and other contaminants. As long as you aren't shivering or sweating you should be fine though.

Do not add any heat, as this, vastly more times than not, will lead to catastrophe rather than that perfect equilibrium you are searching for.

Just as an added note: make sure to use an SAB (still air box) when innoculating/agar work/transfers- makes a world of difference!


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


Extras: Filter Print Post Top
OfflineMush-Room
Stranger
Registered: 07/14/15
Posts: 20
Last seen: 2 years, 5 months
Re: To incubate or not to [Re: TravelAgency]
    #21950092 - 07/16/15 12:04 AM (8 years, 6 months ago)

@arpeggiator- thanks that makes sense I'll give it a shot. Can anyone else confirm this?
@TravelAgency I tried a SAB but with the one i made i could hardly tell what i was doing so i ended up doing it in the open air. I did it ina small bathroom and took a hot shower before hand then sprayed it down with lysol and used the vent while I inoculated. I also heated the needle before each jar.

Can anyone point me towards a good image hosting site? I noticed my pics were deleted already.

Also just throwing it out there... it's pretty much impossible to use bbcode on a phone which is why i didn't.


Edited by Mush-Room (07/16/15 12:06 AM)


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: To incubate or not to [Re: Mush-Room]
    #21950211 - 07/16/15 01:10 AM (8 years, 6 months ago)

Quote:

Mush-Room said:
@arpeggiator- thanks that makes sense I'll give it a shot. Can anyone else confirm this?
@TravelAgency I tried a SAB but with the one i made i could hardly tell what i was doing so i ended up doing it in the open air. I did it ina small bathroom and took a hot shower before hand then sprayed it down with lysol and used the vent while I inoculated. I also heated the needle before each jar.

Can anyone point me towards a good image hosting site? I noticed my pics were deleted already.

Also just throwing it out there... it's pretty much impossible to use bbcode on a phone which is why i didn't.





I had to get a bigger one:



It has been totally worth it, plenty of room.


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


Extras: Filter Print Post Top
OfflineXochi Phil
loading pins


Registered: 12/09/14
Posts: 106
Last seen: 7 years, 6 months
Re: To incubate or not to [Re: Mush-Room]
    #21950263 - 07/16/15 01:37 AM (8 years, 6 months ago)

My grow room is actually the coldest room in the house by as much as four degrees. I keep it around 66-70 degrees because I like things cold myself. Never noticed my jars colonizing slowly. Still takes about two weeks to two and a half.

Darkness is not necessary either.I have thirty or forty jars between a few low powered lights and have noticed better colonization than I had before by a week's time. The mycellium is the organsim and uses a circadian rythym.

Incubation is outdated jazz I believe. You will have nicer stems(dense/meaty) with colder temps in the grow area,and no weird contams as long as you are not walking in there with filthy hands.

Good luck figuring what works best for you. I'd boil that slime jar and then toss the contents and cut your loss.


Extras: Filter Print Post Top
OfflineXochi Phil
loading pins


Registered: 12/09/14
Posts: 106
Last seen: 7 years, 6 months
Re: To incubate or not to [Re: Mush-Room]
    #21950271 - 07/16/15 01:43 AM (8 years, 6 months ago)

I forgot to mention about still air boxes. I find that using one of the six dollar clamp lights from Walmart  attached to the box or just pressed against one side will make the box glow nicely. Prop it up on a counter if you like standing while doing the inoculation and turn off the room lights to improve visibility in the box. Never had an issue with open air. But the SAB gives peace of mind.


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: To incubate or not to [Re: Mush-Room]
    #21951114 - 07/16/15 09:34 AM (8 years, 6 months ago)

Quote:

Mush-Room said:
Can anyone point me towards a good image hosting site? I noticed my pics were deleted already.




http://www.shroomery.org/forums/upload.php?action=upload :super:

I could never see your pics at all.. use the shroomery server, no one wanna click off site links anyway. well maybe someone but you dont want their advice :lol:


Extras: Filter Print Post Top
OfflineDarkhome
•Shaman•N•Training•
Male User Gallery


Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: To incubate or not to [Re: Xochi Phil]
    #21951375 - 07/16/15 10:34 AM (8 years, 6 months ago)

Quote:

Xochi Phil said:
My grow room is actually the coldest room in the house by as much as four degrees. I keep it around 66-70 degrees because I like things cold myself. Never noticed my jars colonizing slowly. Still takes about two weeks to two and a half.

Darkness is not necessary either.I have thirty or forty jars between a few low powered lights and have noticed better colonization than I had before by a week's time. The mycellium is the organsim and uses a circadian rythym.

Incubation is outdated jazz I believe. You will have nicer stems(dense/meaty) with colder temps in the grow area,and no weird contams as long as you are not walking in there with filthy hands.

Good luck figuring what works best for you. I'd boil that slime jar and then toss the contents and cut your loss.



:whathesaid:
I give them a 12 hour on 12 hour off light cycle with A 6500k "daylight" colored fluorescent bulb... I've noticed faster colonization in both grains and jars...leaving them in the dark is outdated info


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Edited by Darkhome (07/16/15 10:38 AM)


Extras: Filter Print Post Top
OfflineMush-Room
Stranger
Registered: 07/14/15
Posts: 20
Last seen: 2 years, 5 months
Re: To incubate or not to [Re: Darkhome]
    #21954437 - 07/17/15 12:07 AM (8 years, 6 months ago)

Here's some pics of the "slime" jar




It finally seems to be producing a Web now though.



And this is one of the jars that started out like it

Date supposed to be 7-12. I've been marking growth daily


Edited by Mush-Room (07/17/15 12:20 AM)


Extras: Filter Print Post Top
OfflineXochi Phil
loading pins


Registered: 12/09/14
Posts: 106
Last seen: 7 years, 6 months
Re: To incubate or not to [Re: Mush-Room]
    #21954562 - 07/17/15 01:29 AM (8 years, 6 months ago)

I am not able to zoom on the image of your slime jar well on my mobile eh. Sometimes you can end up with some odd amount of metabolites. Adding a bit of gypsum(crushed drywall) to jars and again for your bulk sub seems to do the trick,assuming that is what your problem is. Just go for another round of jars and keep them in proper temps. SAB if you can spend another sevenish bucks, and see how these jars develop. Best to not salvage iffy jars if you're unsure.


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: To incubate or not to [Re: Mush-Room] * 1
    #21954622 - 07/17/15 02:33 AM (8 years, 6 months ago)

Quote:

Mush-Room said:
this is not my first grow, however it will be the first time I fruit



:lolwut:


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Incubation Chamber Temperature merlink 1,632 3 08/11/03 09:51 AM
by Psilocybin_monkey
* Incubator For Dummies
( 1 2 all )
indicaz 3,973 23 05/23/02 06:54 PM
by indicaz
* How long is to long to incubate? Jaester 1,342 2 08/30/03 11:52 AM
by dog
* Varying temperature...good...or really bad? TEMP Regulation help.. "WITH PICS" twistedmetal33 690 6 02/28/05 02:46 PM
by george castanza
* Temperature Questions.... DannyBoy 1,470 6 02/24/03 04:46 AM
by Anno
* Easy and cheap Fridge Incubator TEK
( 1 2 all )
Colonel Kurtz Ph.D 18,344 26 02/12/05 02:38 AM
by lepiota
* Incubation Temperature changes: major problem for mycellium? fIsh in my head 3,194 5 07/26/13 04:13 PM
by Blak
* Pavement?s Incubator and Fruiting Chamber Pavement 1,909 5 06/06/03 03:14 AM
by Amanita_Dreamer

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,424 topic views. 30 members, 227 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 14 queries.