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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Just inoculated...did I mess up already? *DELETED*
#21939031 - 07/13/15 08:12 PM (8 years, 6 months ago) |
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Post deleted by DottoreWolfeReason for deletion: resolved
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Re: Just inoculated...did I mess up already? [Re: DottoreWolfe]
#21939235 - 07/13/15 08:55 PM (8 years, 6 months ago) |
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Alcohol doesn't sterilize. Flame sterilization is the key step. It would have been helpful to wipe the ports but you don't wipe the needle after the flame anyway. You will probably be okay assuming the syringe inoculant is clean. There's not reason to think the syringe inoculant is clean by the way.
Also the superglue and tape is over kill. They are self healing ports. Tape of you want extra security but even that isn't needed uNess the ports are junk.
-------------------- "Wasn't that fun"
Edited by OPB (07/13/15 09:04 PM)
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Re: Just inoculated...did I mess up already? [Re: OPB]
#21939691 - 07/13/15 10:28 PM (8 years, 6 months ago) |
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Quote:
OPB said: Alcohol doesn't sterilize. Flame sterilization is the key step. It would have been helpful to wipe the ports but you don't wipe the needle after the flame anyway. You will probably be okay assuming the syringe inoculant is clean. There's not reason to think the syringe inoculant is clean by the way.
Also the superglue and tape is over kill. They are self healing ports. Tape of you want extra security but even that isn't needed uNess the ports are junk.
Nice. I got my innoculant from someone (who I've used many times in the past) so I'm pretty sure I'm good there. Nevertheless, you really think I have nothing to worry about because I forgot to wipe the injector port?
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
Edited by DottoreWolfe (07/13/15 11:06 PM)
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Re: Just inoculated...did I mess up already? [Re: DottoreWolfe]
#21939720 - 07/13/15 10:34 PM (8 years, 6 months ago) |
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You shouldn't name sources. Also it doesn't really matter who the syringe came from. Syringes are made from spore prints and spore prints are very very rarely only the spores you are after. You still have a good chance of being fine on the syringe end but its always a gamble. You will almost certainly have contams but often times the mycelium will overwhelm them. As for the ports its hard to say because I have no idea if they were ever clean or what they have been through. You you pieced them while the needle was still hot and re-flamed between every bag that would certainly help.
In the end there is nothing you can do. Did you mess up? Yes. Will it be disastrous? Not necessarily and you will just have to find out. Once the deed is done its done. No point in worrying just remember this lesson for next time.
-------------------- "Wasn't that fun"
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Just inoculated...did I mess up already? [Re: DottoreWolfe]
#21939738 - 07/13/15 10:39 PM (8 years, 6 months ago) |
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You're fine dude. I never wipe my shit either
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Re: Just inoculated...did I mess up already? [Re: azur]
#21939840 - 07/13/15 11:07 PM (8 years, 6 months ago) |
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Awesome thanks guys <3
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Just inoculated...did I mess up already? [Re: azur]
#21939930 - 07/13/15 11:27 PM (8 years, 6 months ago) |
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Quote:
azur said: You're fine dude. I never wipe my shit either
Yet ya pay top dollar for the high end TP
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Just inoculated...did I mess up already? [Re: Pastywhyte]
#21940028 - 07/13/15 11:50 PM (8 years, 6 months ago) |
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