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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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issues with agar...
#21938761 - 07/13/15 07:07 PM (8 years, 6 months ago) |
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Hello everyone,
So I finally started my journey with agar. I am using a no pour tek the pastyplates. Last thursday I made up 7 plates using this recipe.
agar: 2.5g potato flakes: 3.3g Karo: 1.6g water: 1/2 cup
I pressure cooked the plates for 45 mins let it cool over night the next day I used a syringe that I had for over a year and in a SAB put a drop on ea plate. I let the sit in the sab for about 6 hours then moved them to a shelf where they have sat til today.
I then made another batch of 8 plates the recipe I used was a bit different. Still using pastyplate idea I changed up my recipe a bit because the first batch was a bit hard to pour and was solidifying before I got all 8 dishes poured. The recipe for this batch is as follows:
Agar: 2g potato flakes: 3.3g water: 1/2 cup green food coloring: 3drops
I let this batch cool overnight and did the exact same thing with the same syringe. Now I expect to make some screw ups it is my first time working with agar and I am trying to find my perfect balance and what works for me but after 4 days I thought I would of seen some signs of growth by now even if it was contam growth. So far everything seems the same as the day I put a drop of spore solution on the agar. Any Ideas how to get things rolling or do you guys think that I may just have a bunk syringe?
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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No when inoculating from a spore syringe it can take up to 10 days to actually see if germinate. It is there, just be patient.
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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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Re: issues with agar... [Re: Sockadin]
#21938784 - 07/13/15 07:13 PM (8 years, 6 months ago) |
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Thank you wow did not know. I have another batch that I have some prints that I will be doing tomorrow. I am glad to hear that I had not screwed up. I will let them sit on the shelf for a while longer.
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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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So instead of starting a new thread I am gonna bring my old one back to life. I am very new to agar I have only made up 3 batches of it using the no-pour tek by pasty. 4 days ago I used a spore print instead of a syringe, out of all 3 batches I have absolutely no growth. Now I know I was told the syringe could take up to 10 days before signs could be seen but I thought it would take a lot less for the print or at least see a contam by now. If anyone has any advice for me that would be great the print is one I took myself and is a year old and the syringe came from a shroomery vendor and I have had it for a year but dont know how old the syringe really is. They where both left in a tote in a basement until I moved. If pics will help ill do my best to take some.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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What is the temp in that tote? You can just store it on a shelf in the 70s range and it'll colonize just fine, no need to complicate things. And do you have a GE hole on your plates?
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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How long does it take for mycelium to fully colonize a disk of agar?
Just got my first spores germinated on agar along with a clone but it seems really slow. Both were done over 15 days ago and there is hardly any growth.
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Quote:
Mr.Top_Hat said: So instead of starting a new thread I am gonna bring my old one back to life. I am very new to agar I have only made up 3 batches of it using the no-pour tek by pasty. 4 days ago I used a spore print instead of a syringe, out of all 3 batches I have absolutely no growth. Now I know I was told the syringe could take up to 10 days before signs could be seen but I thought it would take a lot less for the print or at least see a contam by now. If anyone has any advice for me that would be great the print is one I took myself and is a year old and the syringe came from a shroomery vendor and I have had it for a year but dont know how old the syringe really is. They where both left in a tote in a basement until I moved. If pics will help ill do my best to take some.
What makes you think a spore print would be faster? They are just dry spores versus spores suspended in water. They need more time.
Quote:
GreenRabbit said: How long does it take for mycelium to fully colonize a disk of agar?
Just got my first spores germinated on agar along with a clone but it seems really slow. Both were done over 15 days ago and there is hardly any growth.
This is a thread jack. Also why do you want full colonization? That's not even a good thing. You want enough to cut from but not so much you can't see contams.
-------------------- "Wasn't that fun"
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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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Quote:
Psilosoulful said: What is the temp in that tote? You can just store it on a shelf in the 70s range and it'll colonize just fine, no need to complicate things. And do you have a GE hole on your plates?
The plates are not in a tote. They are on a shelf I just stored the syringe and the print in a tote for about a year. as for temp I am unsure it was cool in the basement the room that they are in now is about 70-75 degrees and yes there is a 1/4th ge hole i drilled into ea lid and covered with micropore tape.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: issues with agar... [Re: OPB]
#21948733 - 07/15/15 06:18 PM (8 years, 6 months ago) |
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Quote:
OPB said: This is a thread jack. Also why do you want full colonization? That's not even a good thing. You want enough to cut from but not so much you can't see contams.
Germination times were mentioned; my question was relevant. Asking me questions about my setup is further derailing... Full colonization of agar would be nice because a single plate of agar can be cut into several sections which can each be used to colonize a jar of oats.
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OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
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Quote:
GreenRabbit said:
Quote:
OPB said: This is a thread jack. Also why do you want full colonization? That's not even a good thing. You want enough to cut from but not so much you can't see contams.
Germination times were mentioned; my question was relevant. Asking me questions about my setup is further derailing... Full colonization of agar would be nice because a single plate of agar can be cut into several sections which can each be used to colonize a jar of oats.
Related yes. Relevant to the questions of the OP? No. It's okay I was justing pointing it out because it's not good forum manners to jump in someone else's thread and ask a question about your own project. That's all.
OP: You don't need gas exchange for agar. People do agar in sealed up glass dishes all the time. You just need to wait. Give it a good 2 weeks before you start to think they won't germinate.
-------------------- "Wasn't that fun"
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: issues with agar... [Re: OPB]
#21948825 - 07/15/15 06:35 PM (8 years, 6 months ago) |
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Why bother replying to me twice, yet not answer my question...
I don't need a lesson on forum manners lol. Answering my question would have likely helped the OP as well...
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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all myc is not created equal you know rabbit. once it starts going it can grow pretty fast.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 10 hours
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Re: issues with agar... [Re: OPB]
#21948926 - 07/15/15 07:00 PM (8 years, 6 months ago) |
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I usually only PC my agar "plates" for 20-30 minutes. I'm leery of caramelizing the karo or honey.
They seem to work okay though.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
Edited by FreeWorldOrder (07/15/15 07:02 PM)
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Time shouldn't matter when it comes to caramelization; pressure and temperature do.
So are you saying by doing only 20-30 minutes, you PC your agar and stay under 15 psi?
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 10 hours
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Quote:
GreenRabbit said: Time shouldn't matter when it comes to caramelization; pressure and temperature do.
So are you saying by doing only 20-30 minutes, you PC your agar and stay under 15 psi?
No... I PC at 15psi.for 20-30min. max. When I go longer than 30 minutes the agar comes out dark, kinda burnt looking.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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