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Invisibleoontribe
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cloning in grain jar, can i shake? and other questions?
    #21937817 - 07/13/15 03:31 PM (8 years, 6 months ago)

So i took a tissue from a big fast growing fruit and drop it in a jar smaller than a half pint...it took a few days to start colonizing and now it reached 35%.

1-can i shake it?
I want it to colonize fast to avoid contamination!

2-i would like to use the same strain in the future, how can i achieve this, should i g2g to 1 or 2 quart jars and use em as a master jars g2g to others when i want and more g2gs and store the original 2 in the fridge ?
like this:
tissue in the half pint--->g2g to 2 quarts--->g2g to a jar when needed----use this jar as a master g2g to other jars.


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OfflineTheMustardTiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937825 - 07/13/15 03:33 PM (8 years, 6 months ago)

1- you can shake.

2- If you want to preserve a good performing culture, look into making master slants.


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: TheMustardTiger]
    #21937915 - 07/13/15 03:55 PM (8 years, 6 months ago)

Thanks for the info
But iam using grain (millet) not agar!


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937920 - 07/13/15 03:57 PM (8 years, 6 months ago)

you cant shake agar :tongue2:

where did you grow the pin you cloned? or, how did you grow it?


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OfflineTheMustardTiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: spacechildo]
    #21937929 - 07/13/15 03:59 PM (8 years, 6 months ago)

You shouldn't have dropped cloned tissue directly onto sterilized grain. You should have taken the clone to agar, cleaned it up, then dropped healthy mycelium onto sterilized grain.


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OfflineCMOSS
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937934 - 07/13/15 04:00 PM (8 years, 6 months ago)

I would worry about bacteria problems further down the road once you start expanding.  That tissue sample probably isn't 100% clean, so it might be doing well now but over time/transfers the bacteria has time to 'establish' itself.

That sounds good though, put that half pint to 2 quart jars, save one, expand the others, and see what happens.  Probably be fine for one or two transfers.


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: TheMustardTiger]
    #21937941 - 07/13/15 04:01 PM (8 years, 6 months ago)

Lol Its all grain not agar!
well if it didnt contaminate then why not just use grain!
millet spawned to CVG monotub


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937948 - 07/13/15 04:03 PM (8 years, 6 months ago)

if you grew the mushroom in open air I wouldnt put it right into grains.
And I would especially not save those grain jars for use as masters later.


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OfflineTheMustardTiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937953 - 07/13/15 04:04 PM (8 years, 6 months ago)

Quote:

oontribe said:
Lol Its all grain not agar!
well if it didnt contaminate then why not just use grain!
millet spawned to CVG monotub




Fruiting is not a sterile process. Just because you don't see bacteria, spores, etc on your fruits doesn't mean they aren't there. You've effectively transferred dirty tissue to clean, sterilized grain. If it doesn't contaminate I would be really surprised.


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OfflineZhu
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937954 - 07/13/15 04:04 PM (8 years, 6 months ago)

After shaking you will see if you have bact contams or not :smile:


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: spacechildo]
    #21937974 - 07/13/15 04:08 PM (8 years, 6 months ago)

But PF cloned using grains before! Yes it took him alot of jars but it worked and with other people too...and yea of course agar is way better but i cant work with it for now.
i transferred the tissue in a sterile condition but yea they could still be contam!
what if the tissue 100% colonized the jar without any noticeable contam?


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OfflineTheMustardTiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937977 - 07/13/15 04:09 PM (8 years, 6 months ago)

Quote:

oontribe said:
But PF cloned using grains before! Yes it took him alot of jars but it worked and with other people too...and yea of course agar is way better but i cant work with it for now.
i transferred the tissue in a sterile condition but yea they could still be contam!
what if the tissue 100% colonized the jar without any noticeable contam?




Then you got extremely lucky.


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: TheMustardTiger]
    #21937988 - 07/13/15 04:10 PM (8 years, 6 months ago)

Thats why i want to shake it so i can make things go a little bit faster but i dont know if you can shake a jar with a tissue inside it!


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OfflineCMOSS
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21937993 - 07/13/15 04:12 PM (8 years, 6 months ago)

I would just go for it if they look pretty healthy after shaking that half pint, worst thing that happens is you lose some time and grains.  I wouldn't do it if you plan on doing more than a tub or two with it though. 

After the half-pint fully colonizes, shake it up again and see how fast it recovers, should recover quickly if its relatively bacteria free.

When you go for spawning to bulk for the tub, just use a high ratio so it colonizes quickly incase you have some bacteria buddies riding along.  (which you probaly do)


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21938023 - 07/13/15 04:17 PM (8 years, 6 months ago)

Quote:

oontribe said:
Thats why i want to shake it so i can make things go a little bit faster but i dont know if you can shake a jar with a tissue inside it!




of course you can! Why shouldn't you?


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: spacechildo]
    #21938273 - 07/13/15 05:14 PM (8 years, 6 months ago)

Well i just thought that the tissue is more prone to contam if its not surrounded by myc...well iam just learning! Lol


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #21938290 - 07/13/15 05:19 PM (8 years, 6 months ago)

the tissue is myc :cookiemonster:


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Invisibleelasticaltiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: TheMustardTiger]
    #21938459 - 07/13/15 06:02 PM (8 years, 6 months ago)

Quote:

TheMustardTiger said:
You shouldn't have dropped cloned tissue directly onto sterilized grain. You should have taken the clone to agar, cleaned it up, then dropped healthy mycelium onto sterilized grain.




I go from clone to grain all the time. Clones / pins taken straight to agar and then healthy ones dropped directly to grain. Or clone tissue / pins dropped directly onto grains. Plenty of people have high success taking clone tissue directly to grains.

Of course it's more risky.  But for a home grower it's not an invalid way to get clones growing.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlineshellzenone
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Re: cloning in grain jar, can i shake? and other questions? [Re: elasticaltiger]
    #21938490 - 07/13/15 06:05 PM (8 years, 6 months ago)

Try looking into liquid cultures, but honestly, agar is the way to go. Even if your jar is contamed, you can still save that clone using agar!


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: elasticaltiger]
    #21938493 - 07/13/15 06:05 PM (8 years, 6 months ago)

cron taught me to simply stab the pins on agar plates with a flamed syringe needle, rip him up and drop wherever you want. real easy!


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OfflineTheMustardTiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: elasticaltiger]
    #21938503 - 07/13/15 06:07 PM (8 years, 6 months ago)

Quote:

elasticaltiger said:
Quote:

TheMustardTiger said:
You shouldn't have dropped cloned tissue directly onto sterilized grain. You should have taken the clone to agar, cleaned it up, then dropped healthy mycelium onto sterilized grain.




I go from clone to grain all the time. Clones / pins taken straight to agar and then healthy ones dropped directly to grain. Or clone tissue / pins dropped directly onto grains. Plenty of people have high success taking clone tissue directly to grains.

Of course it's more risky.  But for a home grower it's not an invalid way to get clones growing.




With access to agar I can't see myself recommending a method that involves taking tissue from a dirty fruiting chamber and dropping it into sterilized grain. If it works for you, great, but It's not good advice. Every clone I've ever taken needed at least a transfer to get away from whatever was crawling all over it while in the greenhouse.


--------------------


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: shellzenone]
    #21938506 - 07/13/15 06:08 PM (8 years, 6 months ago)

Quote:

shellzenone said:
Try looking into liquid cultures, but honestly, agar is the way to go. Even if your jar is contamed, you can still save that clone using agar!




that would be the worst place to drop a pin that grew in open air! :eek:


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: TheMustardTiger]
    #21938530 - 07/13/15 06:11 PM (8 years, 6 months ago)

Of course agar is more reliable as you can clone further and make sure its clean.


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Invisibleelasticaltiger
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Re: cloning in grain jar, can i shake? and other questions? [Re: spacechildo]
    #21939049 - 07/13/15 08:18 PM (8 years, 6 months ago)

Quote:

spacechildo said:
cron taught me to simply stab the pins on agar plates with a flamed syringe needle, rip him up and drop wherever you want. real easy!




Exactly


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlineshellzenone
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Re: cloning in grain jar, can i shake? and other questions? [Re: spacechildo]
    #21943073 - 07/14/15 05:37 PM (8 years, 6 months ago)

Quote:

spacechildo said:
Quote:

shellzenone said:
Try looking into liquid cultures, but honestly, agar is the way to go. Even if your jar is contamed, you can still save that clone using agar!




that would be the worst place to drop a pin that grew in open air! :eek:




yes it would! I thought he was cloning from a sterile sample. The drawback of LCs is that it can be hard to tell if there's any contams. But in my opinion, once tested, they are a really good way expanding a master culture.


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Offlinespacechildo
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Re: cloning in grain jar, can i shake? and other questions? [Re: shellzenone]
    #21943139 - 07/14/15 05:51 PM (8 years, 6 months ago)

I'd still throw it on agar first :shrug:

checkout bodhisattas threads on whats in a lc.


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: spacechildo]
    #21943316 - 07/14/15 06:27 PM (8 years, 6 months ago)

I shacked my grain jar and its recovering...if the jar became fully colonized and smells like nushroom does it means that its contam free?


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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #22004361 - 07/27/15 12:47 PM (8 years, 6 months ago)



Fully colonized a week+ ago...looks good!


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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #22007975 - 07/28/15 04:05 AM (8 years, 6 months ago)

what you guys think?


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OfflineMachiavelliavore
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Re: cloning in grain jar, can i shake? and other questions? [Re: oontribe]
    #22008005 - 07/28/15 04:38 AM (8 years, 6 months ago)

Looking nice.  I don't understand why they're so mystified that dropping clean inner-stipe tissue onto grain works.  If you can get a clean clone on agar first attempt, you can do it on any medium.

The downside is that it's hard to transfer away from contams on grain, and harder to see them.

I hope you didn't open the jar and sniff it, or you have probably introduced enough contams that it can't be used for g2g/master culture.

If you want to use it as a master culture, just remove a few grains with flamed tweezers in the SAB, and put them into another grain jar.  Observe colonization for signs of contams.  Shake at 20-30%


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleoontribe
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Re: cloning in grain jar, can i shake? and other questions? [Re: Machiavelliavore]
    #22008008 - 07/28/15 04:39 AM (8 years, 6 months ago)

thanks for the info man :smile:


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