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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: insanemike]
    #21941543 - 07/14/15 10:45 AM (8 years, 6 months ago)

Quote:

insanemike said:
Just remember that a flowhood is not a substitute for great sterile technique.



O no, I wouldn't compromise sterile technique as a whole, just use a proper flowhood for A part of it. Thanks for the advice. I going to do some more reading on them in order to incorporate it into what I've learned and utilized to my advantage thus far. Definitely looks promising. Thanks mike.


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contaminants worst enemy. [Re: lovesquare]
    #21941550 - 07/14/15 10:46 AM (8 years, 6 months ago)

If you have any questions on building a flowhood, just pm me.


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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: insanemike]
    #21941562 - 07/14/15 10:48 AM (8 years, 6 months ago)

Quote:

insanemike said:
If you have any questions on building a flowhood, just pm me.



Once I get into it a bit more and start getting things rolling this fall, I'll definitely take you up on the offer, appreciate it brother, +5.


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


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Offlineknomadic_niki
A mile high
 User Gallery


Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 5 months
Re: Contaminants worst enemy. [Re: lovesquare]
    #21941612 - 07/14/15 11:01 AM (8 years, 6 months ago)

Quote:

lovesquare said:
Quote:

knomadic_niki said:
you are most certainly NOT eliminating airborn contams with oust. why don't you believe 10 people with tons of experience? if you stopped buying hand sanitizer and oust, you could afford a flow hood and actually produce nearly sterile air. in the mean time, you don't need it to use an SAB. save your cash, build a flow hood soon



I wouldn't say I'm completely eliminating them, but definitely reducing the amount of contams inside and outside the SAB, all though the hand sanitizer mega bottle at costco goes a long way, as well as the glade+oust at lowes, I will be investing in a flow hood for my upcoming endeavors in edibles/medicinals. It's not all that expensive but I do agree that flow hoods are the BEST when it comes to sterile techniques as a whole. I'm just simply utilizing what has worked for me, and all though I could change now, I simply could'nt fathom with dealing with contams, as I have avoided completely up to now. I believe my technique and properly releasing endospores and following the correct soaking/boiling/pressure cooking preparation for grains contributes to my success.




lovesquare said:
Basically I spray the transfer room and adjacent rooms with glade+oust, extremely thoroughly to eliminate airborne contams,




Edited by knomadic_niki (07/14/15 12:53 PM)


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Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: Contaminants worst enemy. *DELETED* [Re: knomadic_niki]
    #21941750 - 07/14/15 11:52 AM (8 years, 6 months ago)

Post deleted by stevo

Reason for deletion: .


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OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Contaminants worst enemy. [Re: stevo]
    #21941756 - 07/14/15 11:53 AM (8 years, 6 months ago)

Quote:

stevo said:
lovesquare, I admit I kind of skimmed over this post, so I dont know if its been mentioned as to WHY the soapwater is so effective. The very first thing I do when busting out the still air box is spray it down with lysol.  Im usually suited up and sitting down at that point and I happen to normally have lysol around, so I give it a "whore bath" for safe measure..  Its good shit IMO even though some say I am being anal.  Its cheap and why not I say.  Anyway, the 2nd thing I do is spray it with soapwater.  The reason soapwater is so great is because the box stays wet way longer than lysol, oust, oust and so on which dries up right away.  By the time I have got all my shit ready and my hands in there, there is no disinfectant at all in the box.  I often run out of disinfectants and still keep on nocing up jars.  Ive never noticed any lesser of a success rate.  If you ever wind up doing 16 quarts a nighy for a month straight you will see what I mean about the annoyance of having to reapply disinfectelant sprays to the walls of the SAB more frequently.



don't waste your time. OP is a troll.


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Offlineknomadic_niki
A mile high
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Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 5 months
Re: Contaminants worst enemy. [Re: Psilosopherr]
    #21941964 - 07/14/15 12:55 PM (8 years, 6 months ago)

i challenge love square to do his/her same preparation procedure without oust and hand sanitizer, just soap/water inside the SAB, and clean hands and tools (i use 90% iso in a spray bottle but i suppose hand sanitizer would work for hands and tools). then, open 10 agar dishes inside the SAB as if he/she were doing a transfer. report your results back here


--------------------
My trade list

In search of sporeless oyster cultures


Edited by knomadic_niki (07/14/15 01:02 PM)


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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: stevo]
    #21941969 - 07/14/15 12:57 PM (8 years, 6 months ago)

Quote:

stevo said:
lovesquare, I admit I kind of skimmed over this post, so I dont know if its been mentioned as to WHY the soapwater is so effective. The very first thing I do when busting out the still air box is spray it down with lysol.  Im usually suited up and sitting down at that point and I happen to normally have lysol around, so I give it a "whore bath" for safe measure..  Its good shit IMO even though some say I am being anal.  Its cheap and why not I say.  Anyway, the 2nd thing I do is spray it with soapwater.  The reason soapwater is so great is because the box stays wet way longer than lysol, oust, oust and so on which dries up right away.  By the time I have got all my shit ready and my hands in there, there is no disinfectant at all in the box.  I often run out of disinfectants and still keep on nocing up jars.  Ive never noticed any lesser of a success rate.  If you ever wind up doing 16 quarts a nighy for a month straight you will see what I mean about the annoyance of having to reapply disinfectelant sprays to the walls of the SAB more frequently.



Yeah i get what your saying, the hand sanitizer seems to stay "wet" on the walls for a while as well, and I agree lysol and oust wouldn't go a long way as far as keeping contams sticking to them as a surface disinfectant. I'm just utilizing it for the air and rely on the hand sanitizer (heeping portions might I add) for the surface stickiness for contams, as well as sanitizing my jars, lids and rings. I'm going to try soap water in my upcoming edible grow as it does seem far superior to hand sanitizer for what I'm using it for, and I won't deny that.


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: knomadic_niki]
    #21941995 - 07/14/15 01:04 PM (8 years, 6 months ago)

Quote:

knomadic_niki said:
i challenge love square to do his/her same preparation procedure without oust and hand sanitizer, just soap/water inside the SAB, and clean hands and tools. then, open 10 agar dishes inside the SAB as if he/she were doing a transfer. report your results back here



Once I dive into agar and edibles this fall, I'm going to be branching out from my usual technique due to the overwhelming support of soap water and I'll keep everyone updated on my success rate as far as fine tuning my procedure goes. I just wanted to reiterate what has worked for me and hopefully help other at the same time, as this was done in an old house, (luckily without carpet), and now that I have a great grow room, (that I'm going to clean top to bottom and take necessary precautions from everything that could possibly go wrong), I hope to take advantage of the advice here as well as my own research, to absolutely fine tune EVERYTHING, including agar, flowhood, grain to grain and liquid culture, no need to short cut anything, as these are all simple techniques and nothing should deter new growers to experienced growers alike.


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


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Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: Contaminants worst enemy. *DELETED* [Re: Psilosopherr]
    #21942003 - 07/14/15 01:07 PM (8 years, 6 months ago)

Post deleted by stevo

Reason for deletion: .


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Offlineknomadic_niki
A mile high
 User Gallery


Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 5 months
Re: Contaminants worst enemy. [Re: stevo]
    #21942031 - 07/14/15 01:18 PM (8 years, 6 months ago)

i suggested the use of agar because you will be able to see if there are any contaminants within a few days instead of waiting until you expand to bulk sub. it shouldn't require any different of a technique than your g2g process


--------------------
My trade list

In search of sporeless oyster cultures


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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: knomadic_niki]
    #21942093 - 07/14/15 01:39 PM (8 years, 6 months ago)

Quote:

knomadic_niki said:
i suggested the use of agar because you will be able to see if there are any contaminants within a few days instead of waiting until you expand to bulk sub. it shouldn't require any different of a technique than your g2g process



Yeah, this is what interests me in agar, and I'm looking forward to utilizing it for my upcoming grows, along with my current grain to grain technique and also branching out into soap water for a surface disinfectant variant.


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


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InvisibleJuiceh
Dabbing All Day
 User Gallery


Registered: 09/25/12
Posts: 3,208
Re: Contaminants worst enemy. [Re: lovesquare]
    #21942130 - 07/14/15 01:52 PM (8 years, 6 months ago)

Quote:

lovesquare said:
I'll be growing edibles/medicinals in the coming months in my new grow room we recently cleaned out



With lysol+oust spray???! :wink:


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: Juiceh]
    #21942145 - 07/14/15 01:56 PM (8 years, 6 months ago)

Quote:

Juiceh said:
Quote:

lovesquare said:
I'll be growing edibles/medicinals in the coming months in my new grow room we recently cleaned out



With lysol+oust spray???! :wink:



Of course, it works wonders, but won't bother with adjacent rooms because this will be a closed space as is. I may take you all up on the offer and give it a shot without, just for the :lol:


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


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Offlinenewrook
Sucks at bulk
Male User Gallery

Registered: 03/20/15
Posts: 657
Last seen: 3 months, 5 days
Re: Contaminants worst enemy. [Re: lovesquare]
    #21942192 - 07/14/15 02:11 PM (8 years, 6 months ago)

Quote:

lovesquare said:
Quote:

Juiceh said:
Quote:

lovesquare said:
I'll be growing edibles/medicinals in the coming months in my new grow room we recently cleaned out



With lysol+oust spray???! :wink:



Of course, it works wonders, but won't bother with adjacent rooms because this will be a closed space as is. I may take you all up on the offer and give it a shot without, just for the :lol:





And here I thought you were making progress


--------------------

                                                                      THROW AWAY YOUR SGFC


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Contaminants worst enemy. [Re: Mad Season]
    #21942276 - 07/14/15 02:32 PM (8 years, 6 months ago)

Quote:

Mad Season said:
OK we get it you're a troll. Just stop promoting garbage and let this thread die already




--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


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Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: Contaminants worst enemy. *DELETED* [Re: TravelAgency]
    #21942618 - 07/14/15 04:01 PM (8 years, 6 months ago)

Post deleted by stevo

Reason for deletion: .


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Offlinelovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
Re: Contaminants worst enemy. [Re: stevo]
    #21942893 - 07/14/15 04:58 PM (8 years, 6 months ago)

Quote:

stevo said:
Also, in response to sanitizing gloves, jar lids, rings I would recommend ethanol.  Ive never bothered with oust because its like pissing in the wind basically, but for these purposes lysol will really tear up glove and tyvek sleeves and likely a lot of filter material.  I recommend rubbing alcohol.  There is no residue left behind by it.  This is not just something Ive read either.  Ive seen rubber gloves literally vaporize away from the chemical reaction.  So as mentioned Ive never tried the oust, but it isnt a surface disinfectant it is an air sanitizer.



Lysol is 50% Ethanol.


--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.

If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Contaminants worst enemy. [Re: stevo]
    #21942911 - 07/14/15 05:01 PM (8 years, 6 months ago)

Quote:

stevo said:
Also, in response to sanitizing gloves, jar lids, rings I would recommend ethanol.  Ive never bothered with oust because its like pissing in the wind basically, but for these purposes lysol will really tear up glove and tyvek sleeves and likely a lot of filter material.  I recommend rubbing alcohol.  There is no residue left behind by it.  This is not just something Ive read either.  Ive seen rubber gloves literally vaporize away from the chemical reaction.  So as mentioned Ive never tried the oust, but it isnt a surface disinfectant it is an air sanitizer.




I like to Lysol work surfaces and 70% alcohol everything else. Not above 70%- that won't evaporate as easily making it less effective as a sanitizer.


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contaminants worst enemy. [Re: TravelAgency]
    #21943064 - 07/14/15 05:34 PM (8 years, 6 months ago)

I give my flowhood a good 70% iso wipe down before turning it on and then a good quick evaporative spray afterward. I also use germ-x for lids, gloves and scalpel (handel) before each and every transfer because when it dries, it becomes a bit tacky which aids in preventing laminar air from blowing spores or endospores off of my hands or tools when performing sterile work.

(Edited)

I do flame sterilize.


Edited by insanemike (07/14/15 05:58 PM)


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