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OfflinefirstTIMER420
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Spore syringe question
    #21931336 - 07/11/15 11:15 PM (8 years, 6 months ago)

Im going to inoculate some grain jars in a day or two, and I wont be using all the solution in the 12cc syringe and I want to save it, as I am going to be making some BRF jars to spawn in some trays the same day.

Now, I over analyze everything, and im posting this to ask, after im done inoculating the grain jars, I plan on heating the needle tip till its red hot again, then capping the needle back up and leaving it in the SAB till the other jars are ready. 

I might just take the needle off and stick the nipple back on, and then use another needle tip when the time comes to innoc the BRF jars.

What do you guys suggest? and will heating the needle and capping it be sufficient to avoid getting contams in my BRF jar from anything that might be on the needle.



How do YOU guys store a syringe after use?


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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Edited by firstTIMER420 (07/11/15 11:16 PM)


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Invisiblemush madness
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Re: Spore syringe question [Re: firstTIMER420]
    #21931345 - 07/11/15 11:17 PM (8 years, 6 months ago)

In my sock drawer, dark and cool


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OfflinefirstTIMER420
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Re: Spore syringe question [Re: mush madness]
    #21931352 - 07/11/15 11:19 PM (8 years, 6 months ago)

right after using it, what do you do to store the syringe. do you do what im planning on doing?


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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Invisiblemush madness
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Re: Spore syringe question [Re: firstTIMER420]
    #21931364 - 07/11/15 11:22 PM (8 years, 6 months ago)

Flame the needle,  replace cap and store in a dark cool area


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Onlinerxb
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Re: Spore syringe question [Re: firstTIMER420]
    #21931369 - 07/11/15 11:23 PM (8 years, 6 months ago)

i knock up an agar jar using this tek

http://www.shroomery.org/forums/showflat.php/Number/21899443

let it colonize

and stick it in the fridge.

and stick the rest of the syringe in the fridge to trade to people later.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Offlinetryptkaloids
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Re: Spore syringe question [Re: rxb]
    #21931375 - 07/11/15 11:25 PM (8 years, 6 months ago)

just make sure that you push the cap all the way on. I did this with my lion's mane culture but didn't close it all the way. didn't lose much but I still don't know if it picked up any contams


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
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Pick a book, Make some chips!
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Onlinerxb
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Re: Spore syringe question [Re: tryptkaloids]
    #21931380 - 07/11/15 11:26 PM (8 years, 6 months ago)

if you use agar jars you can just isolate from the contamns... costs almost nothing.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: rxb]
    #21931893 - 07/12/15 02:10 AM (8 years, 6 months ago)

Quote:

rxb said:
if you use agar jars you can just isolate from the contamns... costs almost nothing.




Agreed. I personally believe agar to be the most valuable skill set for mycology.


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OfflinefirstTIMER420
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Re: Spore syringe question [Re: TheEaglesGift]
    #21932558 - 07/12/15 08:56 AM (8 years, 6 months ago)

I plan on starting my agar cultures soon, but your saying before I use the syringe on anything else grow it out on agar to ensure there are no contams.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


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InvisiblePastywhyteMDiscord
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Re: Spore syringe question [Re: firstTIMER420]
    #21932764 - 07/12/15 10:21 AM (8 years, 6 months ago)

Grow it on agar and skip the syringe. Then transfer the faster healthiest growth to be sure its clean. Then use an agar wedge to inoculate. You could put a few drops of ms on agar, have it grow clean, but the syringe still has mold spores in it that mess everything up when you inoculate grains with it. Once you start agar you usually don't go backwards.


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OfflineFreeWorldOrder
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Re: Spore syringe question [Re: mush madness]
    #21933426 - 07/12/15 12:54 PM (8 years, 6 months ago)

Quote:

mush madness said:
Flame the needle,  replace cap and store in a dark cool area





:whathesaid:


--------------------
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Onlinerxb
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Re: Spore syringe question [Re: firstTIMER420]
    #21933911 - 07/12/15 03:12 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
I plan on starting my agar cultures soon, but your saying before I use the syringe on anything else grow it out on agar to ensure there are no contams.




yes,

exactly, you can also pick and choose traits you like.

there are three ways to go with agar.

the cheapest, and maybe the easiest (i think its as easy or easier than my flowhood) is this... http://www.shroomery.org/forums/showflat.php/Number/21899443

or you can build a SAB which is slightly more expensive and opens you up to a bit more contaminant possibility.

or flow hoods, which are large heavy and expencive. but they allow you to open up agar plates without fear of contaminants


so closed not risky...open risky....open not risky.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: rxb]
    #21935334 - 07/12/15 10:07 PM (8 years, 6 months ago)

rxb, I don't think that method is any less prone to contamination to opening a jar/dish in a still air box, and I can explain to you why.

When you inoculate that jar and then let it colonize, won't contaminants in the air settle and be trapped in the polyfil, via passage from the GE holes? Now this isn't an issue initially, but when you inject your needle a second time to collect a culture sample, the needle will come into contact with these contaminants trapped that are trapped within polyfil.

When opening a dish in a sab, the only tool coming into contact with the culture will be a flame sterilize metal object, like a scalpel or x-acto knife.


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Offlinetryptkaloids
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Re: Spore syringe question [Re: TheEaglesGift]
    #21935341 - 07/12/15 10:11 PM (8 years, 6 months ago)

:whathesaid:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Onlinerxb
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Re: Spore syringe question [Re: TheEaglesGift]
    #21936056 - 07/13/15 04:44 AM (8 years, 6 months ago)

Quote:

TheEaglesGift said:
rxb, I don't think that method is any less prone to contamination to opening a jar/dish in a still air box, and I can explain to you why.

When you inoculate that jar and then let it colonize, won't contaminants in the air settle and be trapped in the polyfil, via passage from the GE holes? Now this isn't an issue initially, but when you inject your needle a second time to collect a culture sample, the needle will come into contact with these contaminants trapped that are trapped within polyfil.

When opening a dish in a sab, the only tool coming into contact with the culture will be a flame sterilize metal object, like a scalpel or x-acto knife.




i dont KNOW... But i dont think so, i havent had any issues with contamination at all in 30 or so trials. furthermore some plates have recolonized after having water added and removed. i suppose there needs to be testing done with the system someplace other than MY lab to be sure. the results i've had have been quite enjoyable. and i think that it might be better, to use a soft rubber lid with a SHIP ... but that will increase the cost factor and like i said i've  not had 1 contaminated plate and i've been playing with this for a while.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/13/15 04:51 AM)


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Onlinerxb
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Re: Spore syringe question [Re: rxb]
    #21936075 - 07/13/15 05:01 AM (8 years, 6 months ago)

i guess maybe the long term storage tests will show more. :shrug: we shall see.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Spore syringe question [Re: TheEaglesGift]
    #21936083 - 07/13/15 05:11 AM (8 years, 6 months ago)

what do you think would be the best way to test your theory?


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Extras: Filter Print Post Top
Offlinetryptkaloids
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Re: Spore syringe question [Re: rxb]
    #21936549 - 07/13/15 09:31 AM (8 years, 6 months ago)

purposly add trich spores to your polyfill


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: rxb]
    #21936756 - 07/13/15 10:47 AM (8 years, 6 months ago)

Quote:

rxb said:
Quote:

TheEaglesGift said:
rxb, I don't think that method is any less prone to contamination to opening a jar/dish in a still air box, and I can explain to you why.

When you inoculate that jar and then let it colonize, won't contaminants in the air settle and be trapped in the polyfil, via passage from the GE holes? Now this isn't an issue initially, but when you inject your needle a second time to collect a culture sample, the needle will come into contact with these contaminants trapped that are trapped within polyfil.

When opening a dish in a sab, the only tool coming into contact with the culture will be a flame sterilize metal object, like a scalpel or x-acto knife.




i dont KNOW... But i dont think so, i havent had any issues with contamination at all in 30 or so trials. furthermore some plates have recolonized after having water added and removed. i suppose there needs to be testing done with the system someplace other than MY lab to be sure. the results i've had have been quite enjoyable. and i think that it might be better, to use a soft rubber lid with a SHIP ... but that will increase the cost factor and like i said i've  not had 1 contaminated plate and i've been playing with this for a while.




If you were to store your jars in a relatively clean tote and/or lab that would certainly increase your success rate. Letting these guys sit out in a dirty bedroom or kitchen wouldn't be the best place to let them colonize. Everything I know about mycology and sterile work tells me that whatever ends up in that filter can definitely end up in that jar. SHIP ports might be better, but I can picture them popping off as you try to manipulate your needle within the port to scrape/suck up some myc.

If you're having success, then that should be all that matters to you.

I do like the fact you can work on these in open air, though I wouldn't feel comfortable doing it. I used to do it in the past, but I've gotten very used to my SAB and do everything in there these days.


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Onlinerxb
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Re: Spore syringe question [Re: tryptkaloids]
    #21937228 - 07/13/15 12:58 PM (8 years, 6 months ago)

Quote:

tryptkaloids said:
purposly add trich spores to your polyfill




well i can put some blue mold on top, i dont have any trich cultures... i have a couple of house hold mold cultures (and can get more with open plates)... i hesitate to put them on the top without somehow isolating the jar from the lab tho :smile:...as i dont really want a heavily contaminated lab.

i may setup a plastic clean room and try it... not anytime soon probably.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Spore syringe question [Re: TheEaglesGift]
    #21937237 - 07/13/15 01:01 PM (8 years, 6 months ago)

Quote:

TheEaglesGift said:
Quote:

rxb said:
Quote:

TheEaglesGift said:
rxb, I don't think that method is any less prone to contamination to opening a jar/dish in a still air box, and I can explain to you why.

When you inoculate that jar and then let it colonize, won't contaminants in the air settle and be trapped in the polyfil, via passage from the GE holes? Now this isn't an issue initially, but when you inject your needle a second time to collect a culture sample, the needle will come into contact with these contaminants trapped that are trapped within polyfil.

When opening a dish in a sab, the only tool coming into contact with the culture will be a flame sterilize metal object, like a scalpel or x-acto knife.




i dont KNOW... But i dont think so, i havent had any issues with contamination at all in 30 or so trials. furthermore some plates have recolonized after having water added and removed. i suppose there needs to be testing done with the system someplace other than MY lab to be sure. the results i've had have been quite enjoyable. and i think that it might be better, to use a soft rubber lid with a SHIP ... but that will increase the cost factor and like i said i've  not had 1 contaminated plate and i've been playing with this for a while.




If you were to store your jars in a relatively clean tote and/or lab that would certainly increase your success rate. Letting these guys sit out in a dirty bedroom or kitchen wouldn't be the best place to let them colonize. Everything I know about mycology and sterile work tells me that whatever ends up in that filter can definitely end up in that jar. SHIP ports might be better, but I can picture them popping off as you try to manipulate your needle within the port to scrape/suck up some myc.

If you're having success, then that should be all that matters to you.

I do like the fact you can work on these in open air, though I wouldn't feel comfortable doing it. I used to do it in the past, but I've gotten very used to my SAB and do everything in there these days.




i have 30 inoculations and 30 transfers at least.

no contamination. over about 6 months.

i think this is pretty good.

a rubber lid with a ship might be better. but since this is so easy and so effective and so cheap ... (and if it did contaminate you can isolate)... im not sure why it would be worth it.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Onlinerxb
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Re: Spore syringe question [Re: rxb]
    #21937248 - 07/13/15 01:02 PM (8 years, 6 months ago)

er not a ship, but a commercial filter. the rubber lid would be the ship.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Extras: Filter Print Post Top
Offlinetryptkaloids
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Re: Spore syringe question [Re: rxb]
    #21937438 - 07/13/15 01:54 PM (8 years, 6 months ago)

just my idea of testing the problem pointed out. if you saturate the polyfill with any contam(it'll work better if you test with a variety of contams) and still get a clean culture you will know that the polyfil didn't transfer the contam to the syringe


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Onlinerxb
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Re: Spore syringe question [Re: tryptkaloids]
    #21937444 - 07/13/15 01:56 PM (8 years, 6 months ago)

well if i saturate the LID that would make sense, if i saturate the FILL then the fill cant do its job, and the system wouldnt be tested because we'd be breaking it?

you see?

if i have an airlock fan at my door to keep bugs out...and i put bugs in a box and walk them through the airlock... then we cant say the airlock broke...


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/13/15 01:57 PM)


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: tryptkaloids]
    #21937562 - 07/13/15 02:31 PM (8 years, 6 months ago)

Quote:

tryptkaloids said:
just my idea of testing the problem pointed out. if you saturate the polyfill with any contam(it'll work better if you test with a variety of contams) and still get a clean culture you will know that the polyfil didn't transfer the contam to the syringe




Bad idea, regardless of the GE filter being used. No GE filter is 100 percent effective.

I use very high quality industrial cellulose filter discs from aloha culture bank. If I were to literally sprinkle mold and mold spores on the filter, I'm almost certain the contents would contaminate.

Filters aren't meant to stand up to literally being doused with mold spores imo.


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Onlinerxb
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Re: Spore syringe question [Re: TheEaglesGift]
    #21937578 - 07/13/15 02:38 PM (8 years, 6 months ago)

i have no doubt that polyfill is NOT as good as MOST filter discs :smile:


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Extras: Filter Print Post Top
InvisibleTheEaglesGift
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Re: Spore syringe question [Re: rxb]
    #21938615 - 07/13/15 06:33 PM (8 years, 6 months ago)

Quote:

rxb said:
i have no doubt that polyfill is NOT as good as MOST filter discs :smile:




Hey, if you're having great success and you're happy, that's all that should matters.

I used postal tyvek squares taped down with micropore for years til I just wanted to get something nicer and more reliable. The tyvek never let me down, though, despite the fact many bash it.


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Re: Spore syringe question [Re: TheEaglesGift]
    #21938637 - 07/13/15 06:37 PM (8 years, 6 months ago)

i dont think tyvek would work for this tek tho, it might with micropore but that would be more expencive... the polyfills movement is what helps this work. a spore would have to find its way, in the hole, through the maze and out another hole, low probability.

probably as low as your hand creating an eddy current infront of the flow hood and an errant spore landing on a plate.

which is also pretty damned low.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: rxb]
    #21939342 - 07/13/15 09:17 PM (8 years, 6 months ago)

I wasn't saying tyvek or micropore tape would work for your technique, I was just saying I used to use them with good success, despite their somewhat bad rap as GE filter materials.


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OfflineLain-chan
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Re: Spore syringe question [Re: TheEaglesGift]
    #21939359 - 07/13/15 09:20 PM (8 years, 6 months ago)

I usually just put it in the dark side of my fridge, just don't put it in the freezer like my stoned ass did once, Haha I thought someone stole it at first. :tongue:


--------------------
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vs lbh pna ernq guvf, ravtzn.vax VEP zvtug vagrerfg lbh.



Edited by Lain-chan (07/13/15 09:21 PM)


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OfflinefirstTIMER420
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Re: Spore syringe question [Re: rxb]
    #21942240 - 07/14/15 02:23 PM (8 years, 6 months ago)

back to the point guys....


I inoculated my jars today, and I just flamed the needle and then capped it up, couldn't find a nipple, so I guess thatll have to do.


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: firstTIMER420]
    #21942589 - 07/14/15 03:53 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
back to the point guys....


I inoculated my jars today, and I just flamed the needle and then capped it up, couldn't find a nipple, so I guess thatll have to do.




Toss it all in a fresh ziploc. You should be good.


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OfflinefirstTIMER420
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Re: Spore syringe question [Re: TheEaglesGift]
    #21944633 - 07/14/15 11:35 PM (8 years, 6 months ago)

I put the needle in a fresh ziplock, im planning on making some BRF cakes to spawn in some trays tomorrow and I was going to do this to I guess help with contams(if any) In the needle.


Before using it, flame it, squirt a few drops out, then flame again, then innoc.  Think that would help anything?

just throwing this out there...
also, for a 6qt tray im going to use a 2:4 Spawn:sub
and for the 15qt using a 4:11 ratio(or should I go with 5:10 or 4:9)


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: firstTIMER420]
    #21944691 - 07/14/15 11:52 PM (8 years, 6 months ago)

Quote:


Before using it, flame it, squirt a few drops out, then flame again, then innoc.  Think that would help anything?





Squirt first then flame, flaming can drive fluid into your syringe.


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OfflinefirstTIMER420
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Re: Spore syringe question [Re: TheEaglesGift]
    #21947720 - 07/15/15 02:44 PM (8 years, 6 months ago)

Gotcha.
I got 1 more question.
I took out both syringes I had in the fridge and only used 1, then put both back in the fridge for storage. Do you think making them cold then warm then cold will hurt or kill the spores in any way?


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Offlinetryptkaloids
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Re: Spore syringe question [Re: firstTIMER420]
    #21947752 - 07/15/15 02:50 PM (8 years, 6 months ago)

they'll be fine as long as they don't get too hot like 110 is probably on the high side of what they can stand


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InvisibleTheEaglesGift
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Re: Spore syringe question [Re: firstTIMER420]
    #21947910 - 07/15/15 03:21 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
Gotcha.
I got 1 more question.
I took out both syringes I had in the fridge and only used 1, then put both back in the fridge for storage. Do you think making them cold then warm then cold will hurt or kill the spores in any way?




Don't let them freeze(some fridges have zones that can get to freezing, so be careful). Don't let them get too hot(not sure of exact temp for thermal death of spores). Other than that, they'll be fine with the temps you're subjecting them too.


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