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OfflineGolfWangMental
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Registered: 07/10/15
Posts: 13
Loc: Earth
Last seen: 1 year, 2 days
Experimental Grow Process? [HELP][CRITIQUE]
    #21931215 - 07/11/15 10:36 PM (8 years, 6 months ago)

So after about a month of reading and lurking I decided to create an account and wanted to have some people pick apart at my "plan" for Rye Berry Tek.

Experimental Rye Seed Tek:

I Obtain Needed Materials:

II Grain Preparation:
Take 1.5 cups worth of Rye seeds per quart mason jar and place them in large tub.
Using 7 quart mason jars (because that’s all that fits into our PC) uses ~10.5 cups of grain.
Rinse grain using warm / hot water, rinse three to four times, pick out any seeds that rise to the top as well as debris. Clear out as much foreign objects as much as possible.
Bring pot of water enough to contain the seeds to a slight boil / simmer, simmer seeds for 30-45 minutes to allow any possible bacteria to germinate on the seeds (so it can be killed later in the pressure cooking stage). Drain seeds, make sure they’re not soaking / busted, could take time to figure out what heat / water amount / time works best for your settings.


III Mason Jar Preparation:
Put 1.5 cups of hydrated but now dry Rye seeds into each mason jar, make sure you’ve used on of the various gas exchange methods for the lid. (poly-fill / tyvek / self healing ports & filter pieces, etc.)
Cover the top of the jar with the lid, then cover the lid with a doubled up piece of tinfoil to stop excess moisture from entering the jars during pressure cooking.
PC @ 15psi for 45min to 1 hour. Let pressure cooker cool before removing jars.
Shake jars to make sure substrate is loose, not clumpy, free seed movement, etc.


IV Inoculation:
Grain to grain methods work if you know how to do them (using a jar previously colonized to colonize new jars under a flow-hood / glove box to stop contamination.
If using spores / live cultures follow directions stated for such methods, spores 1-2cc per jar.
Shake slightly after inoculation.


V Incubation:
Incubation is an insanely important step in mushroom cultivation and can take anywhere from 4 days to 14 days normally. Keep the jars in a dark room or in a black plastic bag in relatively hot humid conditions. 70%-80% humidity. 70ºf-90ºf.
Shake every few days to speed up mycelium colonization on the free substrate.
Once the jars are completely colonized casing and fruiting can begin.


VI Casing & Other small steps:

60% Coco coir 40% Vermiculite mix.
Make coir as box sates, test wetness of mixed vermiculite and coco coir. Add water to adjust. Should feel moist and at most drip a few drops of water. Should not be soaked. Just wet enough to hold humidity. PC Substrate @ 15pis for 45 – hour.
Take small plastic tubs and fill ½ inch with your casing material, then spoon in your substrate to leave enough room for a ¾ inch - 1 inch casing on top.
Re-incubate small plastic tubs until mycelium growth is even and patching through top casing, do not wait until casing is “OVERLOADED” or you may need to take other approaches to initiate pinning from here.

VII Cultivation:

After the first flush is complete be sure to remove all mushrooms and mushroom material from the casing layer, re hydrate slightly and allow second, third, etc. flush to happen if they happen.

VIII Drying:

Desiccants / fan dry method


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: GolfWangMental]
    #21931244 - 07/11/15 10:44 PM (8 years, 6 months ago)

And that's how you grow mushrooms.


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Offlinerxb
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Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 1 hour, 39 minutes
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: GolfWangMental]
    #21931260 - 07/11/15 10:51 PM (8 years, 6 months ago)

Quote:

GolfWangMental said:
So after about a month of reading and lurking I decided to create an account and wanted to have some people pick apart at my "plan" for Rye Berry Tek.

Experimental Rye Seed Tek:

I Obtain Needed Materials:

II Grain Preparation:
Take 1.5 cups worth of Rye seeds per quart mason jar and place them in large tub.
Using 7 quart mason jars (because that’s all that fits into our PC) uses ~10.5 cups of grain.
Rinse grain using warm / hot water, rinse three to four times, pick out any seeds that rise to the top as well as debris. Clear out as much foreign objects as much as possible.




then soak them over night
Quote:



Bring pot of water enough to contain the seeds to a slight boil / simmer, simmer seeds for 30-45 minutes to allow any possible bacteria to germinate on the seeds (so it can be killed later in the pressure cooking stage). Drain seeds, make sure they’re not soaking / busted, could take time to figure out what heat / water amount / time works best for your settings.




the RR tek from "lets grow mushrooms" would say put them in a colander and then wait until they do not get toilet paper wet
Quote:



III Mason Jar Preparation:
Put 1.5 cups of hydrated but now dry Rye seeds into each mason jar, make sure you’ve used on of the various gas exchange methods for the lid. (poly-fill / tyvek / self healing ports & filter pieces, etc.)
Cover the top of the jar with the lid, then cover the lid with a doubled up piece of tinfoil to stop excess moisture from entering the jars during pressure cooking.
PC @ 15psi for 45min to 1 hour. Let pressure cooker cool before removing jars.
Shake jars to make sure substrate is loose, not clumpy, free seed movement, etc.




you can skip this step and buy prepared jars from out-grow.com for your first batch and see how they should look, you'll get reusable high quality lids with the jars, check mike's bulk discount tab on http://out-grow.com/
Quote:




IV Inoculation:
Grain to grain methods work if you know how to do them (using a jar previously colonized to colonize new jars under a flow-hood / glove box to stop contamination.
If using spores / live cultures follow directions stated for such methods, spores 1-2cc per jar.
Shake slightly after inoculation.




if its a spore syringe shake it up a lot, i suggest spore depot... and you dont need to use much at all.. i would however suggest you use an agar jar http://www.shroomery.org/forums/showflat.php/Number/21899443

and knock that up first... maybe even get a strong isolated strain...and then make a syringe from that.
Quote:



V Incubation:
Incubation is an insanely important step in mushroom cultivation and can take anywhere from 4 days to 14 days normally. Keep the jars in a dark room or in a black plastic bag in relatively hot humid conditions. 70%-80% humidity. 70ºf-90ºf.
Shake every few days to speed up mycelium colonization on the free substrate.
Once the jars are completely colonized casing and fruiting can begin.


VI Casing & Other small steps:

60% Coco coir 40% Vermiculite mix.
Make coir as box sates, test wetness of mixed vermiculite and coco coir. Add water to adjust. Should feel moist and at most drip a few drops of water. Should not be soaked. Just wet enough to hold humidity. PC Substrate @ 15pis for 45 – hour.
Take small plastic tubs and fill ½ inch with your casing material, then spoon in your substrate to leave enough room for a ¾ inch - 1 inch casing on top.
Re-incubate small plastic tubs until mycelium growth is even and patching through top casing, do not wait until casing is “OVERLOADED” or you may need to take other approaches to initiate pinning from here.

VII Cultivation:

After the first flush is complete be sure to remove all mushrooms and mushroom material from the casing layer, re hydrate slightly and allow second, third, etc. flush to happen if they happen.

VIII Drying:

Desiccants / fan dry method




get a dehydrator off of ebay.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/11/15 10:58 PM)


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Offlinerxb
n00b-sabot
 User Gallery


Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 1 hour, 39 minutes
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: rxb]
    #21931265 - 07/11/15 10:54 PM (8 years, 6 months ago)

everything EXCEPT my agar jar tek is at

http://www.mushroomvideos.com/

my agar jar tek can replace EVERYTHING (i think) he says about a flow hood.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Invisiblemush madness
absorbing everything
 User Gallery


Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: GolfWangMental]
    #21931281 - 07/11/15 10:59 PM (8 years, 6 months ago)

Quote:



V Incubation:
Incubation is an insanely important step in mushroom cultivation and can take anywhere from 4 days to 14 days normally. Keep the jars in a dark room or in a black plastic bag in relatively hot humid conditions. 70%-80% humidity. 70ºf-90ºf.
Shake every few days to speed up mycelium colonization on the free substrate.
Once the jars are completely colonized casing and fruiting




70 to 75f and light don't hurt

Shake once at 20 to 30%


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Offlinerxb
n00b-sabot
 User Gallery


Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 1 hour, 39 minutes
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: mush madness]
    #21931286 - 07/11/15 11:00 PM (8 years, 6 months ago)

lol good catch that temp depends on species, but before that matters you'll know how to do your research... thats good for cubes.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Invisiblemush madness
absorbing everything
 User Gallery


Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: rxb]
    #21931294 - 07/11/15 11:02 PM (8 years, 6 months ago)

Quote:

rxb said:
lol good catch that temp depends on species, but before that matters you'll know how to do your research... thats good for cubes.




I was guessing cubes for a first grow


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Offlinerxb
n00b-sabot
 User Gallery


Registered: 08/24/13
Posts: 9,521
Loc: FREE PSYCHONAUTICA
Last seen: 1 hour, 39 minutes
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: mush madness]
    #21931297 - 07/11/15 11:04 PM (8 years, 6 months ago)

Quote:

mush madness said:
Quote:

rxb said:
lol good catch that temp depends on species, but before that matters you'll know how to do your research... thats good for cubes.




I was guessing cubes for a first grow




nod i wasnt correcting YOU just trying to give him future proof info


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


Edited by rxb (07/11/15 11:04 PM)


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Offlineshroomyaxn
Male User Gallery


Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: GolfWangMental]
    #21931317 - 07/11/15 11:10 PM (8 years, 6 months ago)

FYI: I quoted you too many times in my first draft reply, apparently i can only quote 4 times in a reply. Anyway I put " " around what you said, my suggestions are followed.   

"Rinse grain using warm / hot water, rinse three to four times, pick out any seeds that rise to the top as well as debris. Clear out as much foreign objects as much as possible."
I swirl the rye around with my hands or a large spoon, then quickly dump the water out.  This will help get rid of all that dirt and chaff. 


"Bring pot of water enough to contain the seeds to a slight boil / simmer, simmer seeds for 30-45 minutes to allow any possible bacteria to germinate on the seeds (so it can be killed later in the pressure cooking stage)."
I soak my rye in warm water for 12-20 hours with a tablespoon of gypsum. 


"Drain seeds, make sure they’re not soaking / busted, could take time to figure out what heat / water amount / time works best for your settings." 
After the soak is complete I turn the stove on to high and bring the water to a boil for 5-10 minutes.  The reason for this is so when the rye is dumped into a colander it can be steam dried.  Vigorously shake the colander filled with rye until the steam stopps evaporating off the rye. 


"Put 1.5 cups of hydrated but now dry Rye seeds into each mason jar, make sure you’ve used on of the various gas exchange methods for the lid. (poly-fill / tyvek / self healing ports & filter pieces, etc.)"
Use synthetic filter disks, SFD's. 


"Shake slightly after inoculation."
Do not shake after an MS inoculation. 


"...in relatively hot humid conditions. 70%-80% humidity. 70ºf-90ºf."
No. Room temp is fine. 75 is great.  Humidity not relevant at this step. 


"Shake every few days to speed up mycelium colonization on the free substrate.
Once the jars are completely colonized casing and fruiting can begin."
No.  Shake at 20-30%.


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OfflineGolfWangMental
Modest Mouse

Registered: 07/10/15
Posts: 13
Loc: Earth
Last seen: 1 year, 2 days
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: mush madness]
    #21932950 - 07/12/15 11:08 AM (8 years, 6 months ago)

Quote:

mush madness said:
Quote:

rxb said:
lol good catch that temp depends on species, but before that matters you'll know how to do your research... thats good for cubes.




I was guessing cubes for a first grow





Yeah B+'s, the area is going to be constantly around 75-80 so I'm just going to leave it at that this first time. Eventually I'll set up a DubTub with a fish warmer.


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OfflineGolfWangMental
Modest Mouse

Registered: 07/10/15
Posts: 13
Loc: Earth
Last seen: 1 year, 2 days
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: shroomyaxn]
    #21932966 - 07/12/15 11:10 AM (8 years, 6 months ago)

Quote:

shroomyaxn said:
FYI: I quoted you too many times in my first draft reply, apparently i can only quote 4 times in a reply. Anyway I put " " around what you said, my suggestions are followed.   

"Rinse grain using warm / hot water, rinse three to four times, pick out any seeds that rise to the top as well as debris. Clear out as much foreign objects as much as possible."
I swirl the rye around with my hands or a large spoon, then quickly dump the water out.  This will help get rid of all that dirt and chaff. 


"Bring pot of water enough to contain the seeds to a slight boil / simmer, simmer seeds for 30-45 minutes to allow any possible bacteria to germinate on the seeds (so it can be killed later in the pressure cooking stage)."
I soak my rye in warm water for 12-20 hours with a tablespoon of gypsum. 


"Drain seeds, make sure they’re not soaking / busted, could take time to figure out what heat / water amount / time works best for your settings." 
After the soak is complete I turn the stove on to high and bring the water to a boil for 5-10 minutes.  The reason for this is so when the rye is dumped into a colander it can be steam dried.  Vigorously shake the colander filled with rye until the steam stopps evaporating off the rye. 


"Put 1.5 cups of hydrated but now dry Rye seeds into each mason jar, make sure you’ve used on of the various gas exchange methods for the lid. (poly-fill / tyvek / self healing ports & filter pieces, etc.)"
Use synthetic filter disks, SFD's. 


"Shake slightly after inoculation."
Do not shake after an MS inoculation. 


"...in relatively hot humid conditions. 70%-80% humidity. 70ºf-90ºf."
No. Room temp is fine. 75 is great.  Humidity not relevant at this step. 


"Shake every few days to speed up mycelium colonization on the free substrate.
Once the jars are completely colonized casing and fruiting can begin."
No.  Shake at 20-30%.





THANK YOU!


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Invisiblemush madness
absorbing everything
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Registered: 05/22/15
Posts: 252
Loc: Brazil
Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: GolfWangMental]
    #21933192 - 07/12/15 12:02 PM (8 years, 6 months ago)

Quote:

GolfWangMental said:
Quote:

mush madness said:
Quote:

rxb said:
lol good catch that temp depends on species, but before that matters you'll know how to do your research... thats good for cubes.




I was guessing cubes for a first grow





Yeah B+'s, the area is going to be constantly around 75-80 so I'm just going to leave it at that this first time. Eventually I'll set up a DubTub with a fish warmer.




You dont need the heater for anything,  room temperature is perfect for every aspect of growing cubes


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OfflineGreen Bastard
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Registered: 02/26/14
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Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: GolfWangMental]
    #21933287 - 07/12/15 12:23 PM (8 years, 6 months ago)

Simmer for 30-45 minutes??
Wow, sounds like those are gonna be a mushy mess. I soak for 12-18 hours, and bring to a simmer, not full boil, for around 5-10 minutes maximum. I drain in colanders, then spread out on large window screens, supported by "under bed" storage totes, mixing every 5 minutes, until cold and dry.

Then again, I use rye berries. Not sure how rye seeds vary...


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Offlineshroomyaxn
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Registered: 11/13/11
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Re: Experimental Grow Process? [HELP][CRITIQUE] [Re: Green Bastard]
    #21936530 - 07/13/15 09:22 AM (8 years, 6 months ago)

The reason I have found boiling the rye berries helps is purely for the immediate next step, the steam dry shaking step. 

When i used to use WBS I never steam dried the grains, I would just lay them out and let them air dry.  This would always take an hour plus for me.

After boiling the rye and dumping them into a wire colander, they are obviously very hot.  After 5-10 minutes of vigorously shaking the colander the surface of the grains are almost completely dry.


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