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OfflineXiaoYi
Zhu Ren


Registered: 05/17/15
Posts: 19
Last seen: 7 years, 1 month
V-Tek invitro Casing
    #21929813 - 07/11/15 05:17 PM (8 years, 6 months ago)

Greetings all,

I'm preparing to case a couple of V-Tek style RGS containers and fruit them in a SGFC. Violet himself advocates a potting soil casing layer, but I've found potting soil to be an unpopular casing material here on the shroomery. so I decided to try casing my containers with 50/50+ from what I've gathered this is how it's done:

I have Jiffy mix seed starter (no fertilizer) to which I will add verm to make it an even 50/50 Mix of Verm/Peat.

Now I understand that JiffyMix is ph balanced around 6.5 so I will calculate the appropriate amount of lime to bring that up to around 7.5 (I have a soil ph Test Kit)

I will also add gypsum at a 10% ratio to peat by volume however I've read conflicting information as to whether Gypsum raises, lowers, or has no effect whatsoever on ph. I'd appreciate if someone could clear the air.

finally when I have a 50/50 mix with a ph ~7.5 I will pasteurize it before applying to my colonized RGS containers (this is invitro-style) and popping them in the sgfc.

This is what I've come up with after about 3 hours of reading on the forums, if anyone sees anything that could be tweaked please inform me

Thanks!

******UPDATE 7/24/2015**********

*UPDATE*

Hello guys, I just wanted to update in case anyone was interested in how this worked out.

Tall container cased with Verm/Gypsum fruited invitro style* first flush yielded ~50 grams




50/50+ cased containers fruited in SGFC developing pins*




and the small Mini-Round* 50/50+ casing



And here's my first pf cakes on their second flush:

Left side is Golden Teacher and the right side with 3 mushrooms is Amazonian.


Amazonian closeup


Thanks guys, I'd love to hear any suggestions.


Edited by XiaoYi (07/24/15 07:41 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: V-Tek invitro Casing [Re: XiaoYi]
    #21929823 - 07/11/15 05:20 PM (8 years, 6 months ago)

gypsum doesn't do nearly anything for pH :thumbup:

otherwise you're set. potting soil can work as a casing and I've used it before but I personally like to use straight vermiculite these days.


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OfflineXiaoYi
Zhu Ren


Registered: 05/17/15
Posts: 19
Last seen: 7 years, 1 month
Re: V-Tek invitro Casing [Re: bodhisatta]
    #21929900 - 07/11/15 05:40 PM (8 years, 6 months ago)

Thanks a lot!

I actually posted on Violets thread asking if straight vermiculite would work for this, but I was unable to get an answer from anyone. I have 3 containers ready to go so I will case two in 50/50 and one in verm an see how it goes. Thanks again!


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InvisiblebodhisattaMDiscordReddit
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Re: V-Tek invitro Casing [Re: XiaoYi]
    #21929947 - 07/11/15 05:54 PM (8 years, 6 months ago)

:rockon:


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InvisibleViolet
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Re: V-Tek invitro Casing [Re: bodhisatta]
    #21930620 - 07/11/15 08:07 PM (8 years, 6 months ago)

Where have you seen that soil is unpopular? It's not commonly suggested, but I haven't seen it suggested against.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflineXiaoYi
Zhu Ren


Registered: 05/17/15
Posts: 19
Last seen: 7 years, 1 month
Re: V-Tek invitro Casing [Re: Violet]
    #21938039 - 07/13/15 04:20 PM (8 years, 6 months ago)

Sorry haven't been on the forums for a while.

While researching casing layers and different variations I often saw people advocating verm/coir or 50/50+ casing layers and one thread in particular (which I'll link to if I can find it) where a few people had problems with trich on potting soil. Now that I think about it they may have been using soil straight from the bag, without any prep. I'm just being overly cautious as I'm still new to this.

Anyways after messing around with pasteurizing the 50/50+ casing, I can definitely see the merit in your potting soil prep technique as it is a lot faster and convenient. I'll be sure to try it in the future.

---As an aside I'll be applying those 50/50+ casings today. I'll be sure to update when I start getting pins and such.


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OfflineRictus
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Registered: 06/08/15
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Re: V-Tek invitro Casing [Re: XiaoYi]
    #21938147 - 07/13/15 04:46 PM (8 years, 6 months ago)

I would just suggest getting oven safe turkey bags and bake the amount of verm you would like to use at 325F for at least 30 minutes. I have read it is recommend to bake it wet. If your going to bake it moistened make sure it can ventilate built up vapor or you'll have a mess on your hands.  It works if you bake it dry. Standard process to dry sterilize is 2 hours at 320F, but this is over kill. When you pick up the Turkey bags grab a gallon of distilled water and add directly to the turkey bag until field capacity is met, before or after cooking.

I have read of people having Trich from coir, a 50/50 coir verm sounds like a good casing. I can't imagine a casing like that not working. You might as well just sterilize the coir and verm at the same time in the oven if you go this routine and premix them. Can you post a link to the tek your talking about?


Edited by Rictus (07/13/15 04:55 PM)


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Offlinespacechildo
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Re: V-Tek invitro Casing [Re: Rictus]
    #21938198 - 07/13/15 04:55 PM (8 years, 6 months ago)

Quote:

Rictus said:
Standard process to dry sterilize is 2 hours at 320F, but this is over kill. When you pick up the Turkey bags grab a gallon of distilled water and add directly to the turkey bag until field capacity is met, before or after cooking.

I have read of people having Trich from coir, a 50/50 coir verm sound like a good casing.




in what universe is that standard procedure? :stars:

I suggest you grow more and advice less for a littlewhile :crazy:


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OfflineRictus
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Re: V-Tek invitro Casing [Re: spacechildo]
    #21938234 - 07/13/15 05:05 PM (8 years, 6 months ago)

That is standard for medical. What else did I say wrong according to you?

V-Tek
http://www.shroomery.org/forums/showflat.php/Number/19167498


Edited by Rictus (07/13/15 05:06 PM)


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Offlinespacechildo
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Re: V-Tek invitro Casing [Re: Rictus]
    #21938270 - 07/13/15 05:13 PM (8 years, 6 months ago)

medical what? no one sterilizes verm for 2 hrs in an oven.
distilled water. :thumbdown:
coir grows trich? :lol: very little wants to germinate on coir, whatever grows in your spawn isn't your subs fault.

you're just generally all over the place. thought I should say something. 100 lurkers for every poster.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: V-Tek invitro Casing [Re: Rictus]
    #21938275 - 07/13/15 05:15 PM (8 years, 6 months ago)

I'm a little confused, someone please help me out. If op is fruiting invitro, what is the SGFC for?


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OfflineXiaoYi
Zhu Ren


Registered: 05/17/15
Posts: 19
Last seen: 7 years, 1 month
Re: V-Tek invitro Casing [Re: Rictus]
    #21938294 - 07/13/15 05:20 PM (8 years, 6 months ago)

Rictus-

From my understanding it isn't necessary to sterilize plain vermiculite as it's non-nutritive and won't support the growth of bacteria/competitor molds.

However after reading about the benefits of gypsum on mushroom yields I went ahead and added a bit of gypsum to my vermiculite casing, although I'm not sure if my mushrooms will gain the benefits as it's only in the casing layer and not in the actual substrate. if someone could comment on this I would appreciate it.


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OfflineRictus
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Re: V-Tek invitro Casing [Re: Rictus]
    #21938299 - 07/13/15 05:22 PM (8 years, 6 months ago)

I said 30 minutes, that is why I said the 2 hour standard was over kill. That is medical, no medical personnel sterilize verm, so I agree. I shouldn't have said that. I have heard of cases of it contaminating that doesn't mean that I think it should be avoided. I agree it is most likely the operators fault.

Sounds good spacechildo.


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OfflineXiaoYi
Zhu Ren


Registered: 05/17/15
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Last seen: 7 years, 1 month
Re: V-Tek invitro Casing [Re: insanemike]
    #21938306 - 07/13/15 05:24 PM (8 years, 6 months ago)

Mike-

I am fruiting these invitro, I'm just popping the lids off and putting them in the sgfc to control the humidity more easily.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: V-Tek invitro Casing [Re: Rictus]
    #21938325 - 07/13/15 05:33 PM (8 years, 6 months ago)

If you get contamination on verm, it wasn't from anything that recently germinated. Meaning the contam came from the spawn. SPORES WILL NOT GERMINATE ON A MINERAL. It does not contain any carbs or nitrogen which fuel and support cell growth. So there isn't any reason to pasteurize, bake or sterilize it.


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OfflineSockadin
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Re: V-Tek invitro Casing [Re: XiaoYi]
    #21938339 - 07/13/15 05:36 PM (8 years, 6 months ago)

Vermiculite is definitely nutritious to mycelium. It is also nutritious to competitors. Gypsum is a PH buffer, and can help with yields and swings in the substrate.

Also Trich is actually added to potting soil because it is beneficial to plant development and soil biodiversity, (as well as mycelium is.)

My point was only to show the flawed logic and clarify the posts. I really don't know what you guys were originally chatting about.

Edit: Mike your wrong on that one.


Edited by Sockadin (07/13/15 05:37 PM)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: V-Tek invitro Casing [Re: XiaoYi]
    #21938345 - 07/13/15 05:37 PM (8 years, 6 months ago)

Quote:

XiaoYi said:
Mike-

I am fruiting these invitro, I'm just popping the lids off and putting them in the sgfc to control the humidity more easily.




That is not fruiting invitro. Invitro would be with minimum amount of gas exchange that you can get away with. Which means leaving the lid on slightly loose. As soon as you open that container, it is exposed to the open air. Therefor it loses it's sterility which defeats the purpose of trying to aquire clean clones or cultures from an initro grow.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: V-Tek invitro Casing [Re: Sockadin]
    #21938359 - 07/13/15 05:40 PM (8 years, 6 months ago)

Quote:

Tetramine said:
Vermiculite is definitely nutritious to mycelium. It is also nutritious to competitors. Gypsum is a PH buffer, and can help with yields and swings in the substrate.

Also Trich is actually added to potting soil because it is beneficial to plant development and soil biodiversity, (as well as mycelium is.)

My point was only to show the flawed logic and clarify the posts. I really don't know what you guys were originally chatting about.

Edit: Mike your wrong on that one.




No I'm not. Yes. With enough grain spawn, you can use it as a pseudo bulk substrate but spores do not readily germinate on it because it is not nearly nutritious enough to promote cell growth.


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OfflineXiaoYi
Zhu Ren


Registered: 05/17/15
Posts: 19
Last seen: 7 years, 1 month
Re: V-Tek invitro Casing [Re: insanemike]
    #21938368 - 07/13/15 05:41 PM (8 years, 6 months ago)

If "Invitro" only applies to the practice of fruiting in "semi-sterile" conditions for the purpose of obtaining spore prints or clones, then no this isn't invitro. Perhaps I've mistook the meaning of the term. In any event I'm fruiting these in their original plastic containers- lids off, in a sgfc.



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OfflineSockadin
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Re: V-Tek invitro Casing [Re: XiaoYi]
    #21938379 - 07/13/15 05:44 PM (8 years, 6 months ago)

Again Wrong.

Vermiculite can be consumed and absorbed by Mycelium for it's nutritious properties. Mycelium may not germinate on it, but healthy mycelium will colonize and propagate on it, just like mold will.

http://webmineral.com/data/Vermiculite.shtml#.VaRMaI3bIbw

Incase you were wondering what it was made out of.


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