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OfflinePsilosoulful

Registered: 09/05/14
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Brf Substitute Tek Progress + Suggestions
    #21926612 - 07/10/15 11:10 PM (8 years, 6 months ago)

Watsup Mush Cult Viewers!! I'm very excited to restart my grow since early June. I had to take a short break due to lack of funds :frown: I'm back( currently soaking a good amount of WBS to sterilize tomorrow night!!) and have these brf paste jars that have been sitting around for about two weeks.

They are all fully colonized(i hope the paste isn't too thick). The mycelium is digging into the substrate and seems to have a good hold on it. I was wondering if it would be a good idea to make some liquid inoculant with the fluffy-like mycelium, transfer small sections of rhizo-like growth to more brf paste jars, or transfer sections to grain jars that I'm prepping at the moment?

I was reading about isolating sections of good mycelial growth before moving it to grain jars, but that would take a long time before a pure isolate is revealed. Anyway, I have three of these brf paste jars that look rather healthy and don't want them to go to waste.

I will be starting agar shortly(via Pastywhyte's tek) and want to see what I can do with these jars. I know a lot of growers don't like the agar substitute tek, but it's all I have at the moment. Thanks for the help! :smile:



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OfflineDensePlacebo
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21926717 - 07/10/15 11:54 PM (8 years, 6 months ago)

I'm done all three with moderate success. I personally would wait for agar before I transferred onto brf again but that's not saying you couldn't do that. It only takes a little myc sooooo with three you could probably do all of the above. BTW once I found the right amount of agar to use in pastywhytes tek(I had alot of condensation, just me tho) I love that tek and I've used it for months now.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
    #21927352 - 07/11/15 03:56 AM (8 years, 6 months ago)

Wouldn't honey and water liquid cultures be cheaper then this paste concauction?
Agar to lc, ftw


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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InvisibleGrey
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21927590 - 07/11/15 06:25 AM (8 years, 6 months ago)

You could do limited multispore and just transfer a small section from the fastest growth on the plate.that'll narrow down the diversity a little.


You could put them to grain too. I've never done the brf substitute, but have had success with corn meal mush


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
    #21927807 - 07/11/15 08:00 AM (8 years, 6 months ago)

Quote:

DensePlacebo said:
I'm done all three with moderate success. I personally would wait for agar before I transferred onto brf again but that's not saying you couldn't do that. It only takes a little myc sooooo with three you could probably do all of the above. BTW once I found the right amount of agar to use in pastywhytes tek(I had alot of condensation, just me tho) I love that tek and I've used it for months now.



Yeah i might just try doing all three methods and see which one works best. Hopefully this project goes well, I'm also gonna make a new SAB today! Then PC my grains tonight and do some work in the mornin'! :dancer:


Quote:

PurePleasure said:
Wouldn't honey and water liquid cultures be cheaper then this paste concauction?
Agar to lc, ftw



Never tried that out, but I had tons of brf just sitting around so I figured, why not make some paste jars! Can't wait to get into agar, I'm getting the telephone brand agar powder, is this a good type to use? I've read that some agar contains added sugars and this might interfere with solid growth. Just wanna make sure I got what I need to get started on the right foot.


Quote:

Grey said:
You could do limited multispore and just transfer a small section from the fastest growth on the plate.that'll narrow down the diversity a little.


You could put them to grain too. I've never done the brf substitute, but have had success with corn meal mush



I'll try that method, do you think I'll be able to see some sectoring after the first transfer, or does that usually happen after multiple transfers? Because right now all I see is fluffy mycelium all around the plates, no rhizo growth yet.


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InvisibleBill_Oreilly
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21927818 - 07/11/15 08:04 AM (8 years, 6 months ago)

I don't mean to jack the thread but can someone please tell me once and for all if BRF+verm deceases potency like some people claim? is manure or compost really a better alternative for potency related issues? or are all substrates the same


--------------------
Something there is mysteriously formed,
Existing before Heaven and Earth,
Silent, still, standing alone, unchanging,
All-pervading, unfailing,
I do not know its name; I call it tao.
If forced to give it a name, I call it
Great (ta). Being great, it flows out;
Flowing out means far-reaching;
Being far-reaching, it is said to return.


It's just a shot away..


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InvisibleGrey
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21927829 - 07/11/15 08:10 AM (8 years, 6 months ago)

Telephone Brand Agar agar is great.


Seeing sectoring usually takes at least a few transfers for me.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: Bill_Oreilly]
    #21929412 - 07/11/15 03:49 PM (8 years, 6 months ago)

Quote:

Bill_Oreilly said:
I don't mean to jack the thread but can someone please tell me once and for all if BRF+verm deceases potency like some people claim? is manure or compost really a better alternative for potency related issues? or are all substrates the same



Manure is the ideal substrate to work with, haven't tried it yet but I will be pasteurizing some horse manure when my grain jars are 100% colonized. :awethumb:


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OfflineZeroBoyWD
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21929649 - 07/11/15 04:39 PM (8 years, 6 months ago)

I've done BRF cakes that were the fucking bomb. I didn't get as many flushes or a lot of weight but those cakes were SPENT on what I had. Depends on your conditions really


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Bill_Oreilly] * 1
    #21931664 - 07/12/15 12:36 AM (8 years, 6 months ago)

Quote:

Bill_Oreilly said:
I don't mean to jack the thread but can someone please tell me once and for all if BRF+verm deceases potency like some people claim? is manure or compost really a better alternative for potency related issues? or are all substrates the same



Not really. Some might thrive better on other subs but the potency falls back on mostly genetics


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21932435 - 07/12/15 08:04 AM (8 years, 6 months ago)

UPDATE: Just took some WBS jars out of the PC i had running last night. I'm gonna wait for some of that excess moisture to evaporate before transferring a wedge of my brf jar to it. How do they look so far? I also used a piece of tyvek(kite-making material) over a 3/8" GE hole. In the meantime, it's time to construct my new SAB from a 66 qt sterilite tub using a coffee can. Ahh I love the smell of burning plastic in the mornin' :lolsy:
 
 


Edited by Psilosoulful (07/12/15 08:10 AM)


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21932489 - 07/12/15 08:30 AM (8 years, 6 months ago)

Those look rather wet. Shake them and wait a day if they still look like then i wouldn't even try it. And typically tyvek goes on the outside of your lid. Not nessecarry but still. That doesn't even look like tyvek... also did you cover the lids with foil so the tyvek wouldn't get wet? If not that's your problem. That's probably why they are way to wet and once tyvek gets soaked it's not really a good filter anymore because it opens the pores on it up even more.

Shake them really good and lay the jars on the side that way the water doesn't pool up in the bottom and become a breeding ground for a bacteria. After you shake them, hold the jars in your hand, flip them side ways and hold them up and look at the "bottom" of the side. Now roll it. If you see water tumbling through the grains on the bottom as it turns, probably to moist. The last pic looks wet, the others look borderline saveable.
After the shake, shake it again 12 hr later and then when you wake up tom do what I stated above. Turn it on its side and see if water stays on the bottom as you turn the grains. If so the only thing I could think of is run them in your pc again but only for like 30 45 minutes at 15 psi.
but cover those lids with foil!


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




Edited by PurePleasure (07/12/15 08:36 AM)


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21932546 - 07/12/15 08:52 AM (8 years, 6 months ago)

Thanks for the tips! I did cover the jar lids with foil but they did get kinda wet cuz when I took the foil off there was water droplets sitting on the tyvek. I thought that tyvek was waterproof tho? I'll be switching to SFD's soon, so I won't have this moisture problem. I've read that SFD's were the best to use.

I'll try that method you described and check back later. Hopefully, the grains dry up a bit. BTW I PC'd them for the standard 90 min at 15 psi. Link for the tyvek I used: http://www.amazon.com/Kitemaking-Material-Durable-Lightweight-Breathable/dp/B00G28H9QW/ref=sr_1_1?ie=UTF8&qid=1436712643&sr=8-1&keywords=tyvek


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21932618 - 07/12/15 09:20 AM (8 years, 6 months ago)

Quote:

Psilosoulful said:
Thanks for the tips! I did cover the jar lids with foil but they did get kinda wet cuz when I took the foil off there was water droplets sitting on the tyvek. I thought that tyvek was waterproof tho? I'll be switching to SFD's soon, so I won't have this moisture problem. I've read that SFD's were the best to use.

I'll try that method you described and check back later. Hopefully, the grains dry up a bit. BTW I PC'd them for the standard 90 min at 15 psi. Link for the tyvek I used: http://www.amazon.com/Kitemaking-Material-Durable-Lightweight-Breathable/dp/B00G28H9QW/ref=sr_1_1?ie=UTF8&qid=1436712643&sr=8-1&keywords=tyvek



A little moisture on the tyvek isn't going to ruin it.
I just think putting all that tyvek on the inside was a bad idea because unless you shake it right it's going to get wet with grain water and all the humidity in the bottles.BUT you will probably be fine. Tyvek really isn't water proof. It is to a certain extent. Its meant to be used a moisture barrier on houses but yet let it breathe and vent a little. But once it's Pc'd, it expands the pours a bit more.
But that tyvek you used looks thick.
But yes, sfds are amazing. I still use foil over them to keep from getting soaked even though sfds can deal with alot of moisture.
If you want, I'll send you a disk. You can make like 8 lids with one sfd using a nickle and a sharpy  and cut the outside of the mark to its a tad bigger.
I drill a quarter inch holes in my lids, put red rtv silicone around the hole (a thin but steady smear around the hole, you don't want to have to much because when you push the sfd down the silicone can get under the lid and block up air flow on the other side a little bit.) Then when you push the sfd down go around the edges as if you were caulking it with some more silicone just to insure those bad boys are blocked the fuck off.
Walmart has plastic lids, I think it's a 8 or 9 pack for like 3 or 4 bucks? I forget.
If your interested in that , pm me.


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21932646 - 07/12/15 09:36 AM (8 years, 6 months ago)

Thanks man!! The tyvek is actually outside the lid. I siliconed it on and put some extra on the outside to be on the safe side. I've always ran into contam issues when using filters on the underside of jars. Not sure why vendors recommend putting SFD's under the lid tho? :shrug:

I also got a pack of 12 SFD's coming in the mail, should be here by Monday! :dancer:  Wanted to get a head start on these brf paste jars, since they are at full colonization already.

I'll definitely look into getting plastic jar lids, I think I saw them for a decent price last time I went to WalMart. I'll buy a pack for my next grain jars. These metal jar lids are gonna be a bitch to open up inside a SAB. I tightened the lids before PC'ing so the vacuum inside the jar is gonna be strong. I'll update once I make the transfers.


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21934317 - 07/12/15 04:54 PM (8 years, 6 months ago)

My WBS jars dried up nicely after distributing the moisture in the jar by shaking them vigorously for like 2 minutes!! Time to do some transfers :hamsterdance:




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Offlinespacechildo
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21934327 - 07/12/15 04:57 PM (8 years, 6 months ago)

nicely prepped!

have you done any tranfers of myc from brf sub to brf sub?
your first pic looks off to me (bacteria), try not to have brf paste all over the jar too next time.


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
    #21934413 - 07/12/15 05:20 PM (8 years, 6 months ago)

Quote:

spacechildo said:
nicely prepped!

have you done any tranfers of myc from brf sub to brf sub?
your first pic looks off to me (bacteria), try not to have brf paste all over the jar too next time.



I haven't done any transfers yet, still gotta make a SAB, I have a 66qt sterilite tub that I'm gonna use. Yeah, that first jar looks kinda funky, I'll make sure I transfer sections from the other two healthy brf jars. :grin:


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Offlinespacechildo
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21934431 - 07/12/15 05:26 PM (8 years, 6 months ago)

I'm not 100% but is all your brf paste covered in myc?
you wanna do a transfer as soon as you see some myc germination to try and beat the contam spores from germinating.

if they grew all out just try to take a tiny piece and see what grows.


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
    #21934568 - 07/12/15 06:14 PM (8 years, 6 months ago)

They are all 100% colonized and the mycelium is climbing up the sides. I'll be transferring some sections out tonight. I have 7 WBS jars that I'm gonna use. These are the three brf paste jars I'm using. I'm still debating whether or not to use the jar in the last pic, looks a bit off to me.




Edited by Psilosoulful (07/12/15 06:15 PM)


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21935138 - 07/12/15 09:13 PM (8 years, 6 months ago)

Those jars look great dude. You pulled it off. I would even use the last one.
Also just one of those cultures should be enough to inoculate all 7.
I would transfer out half of one culture to other dishes and use 1 and a half on your jars and keep the other culture in the fridge for a backup plan :thumbup:


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21935222 - 07/12/15 09:34 PM (8 years, 6 months ago)

Thanks man!! I didn't expect such good growth either. This BRF paste tek I found was just something to keep me busy until I could get agar supplies. Now I can get growing and work on my sterile procedures in the SAB. I've always used a glovebox up until now with moderate success.

How long do you think cultures can be stored for in the fridge and will they be just as effective when used again?


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21935585 - 07/12/15 11:28 PM (8 years, 6 months ago)

Quote:

Psilosoulful said:
Thanks man!! I didn't expect such good growth either. This BRF paste tek I found was just something to keep me busy until I could get agar supplies. Now I can get growing and work on my sterile procedures in the SAB. I've always used a glovebox up until now with moderate success.

How long do you think cultures can be stored for in the fridge and will they be just as effective when used again?



I've used cultures a year old.
Trick is is after you take them out the fridge, let them sit out for at least a day or a few days. That will give the myc time to come out of shock and wake back up pretty much.
I believe they become a little less effective after time but nothing to really worry about. Just after it wakes up, make a transfer and then cut out the first piece of quickest expansion and drop it into a other plate. Drop it on grains, get mushrooms, and then start back over with spore prints to agar.
Then clean it up on agar more. It only takes a few transfers usually to get quick, healthy, rhizmorphic growth.
Personally, I let my plates fully colonize, then take the most rhizmorphic piece (usually at the end of the plate) and drop  it on agar. It will just continue to produce more and more rhizmorphic growth. Some people do a million transfers and end up with a monoculture which is one set of genetics and produces and acts the same everytime but most monocultures suck. Get good healthy growth on your plate sand go with the flow :thumbup:


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21935678 - 07/12/15 11:58 PM (8 years, 6 months ago)

Thanks for the info, it's really helpful :thumbup: I just transfered the brf jar with the most rhizo growth to the seven grain jars. Hopefully all goes well and no jars contaminate. For my first time working in my an SAB , i think I did pretty good, followed all the sterile procedures, but there'e always room for improvement.

I'm gonna store the last two brf paste jars in the fridge for now. I wanna see how these grain jars do before I make any other transfers.

I also have some spore prints from my last grow. I might go ahead and make another batch of brf jars and throw that on there and see if I get clean growth. Soo manny options right now!! :grin:


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21935711 - 07/13/15 12:13 AM (8 years, 6 months ago)

Quote:

Psilosoulful said:
Thanks for the info, it's really helpful :thumbup: I just transfered the brf jar with the most rhizo growth to the seven grain jars. Hopefully all goes well and no jars contaminate. For my first time working in my an SAB , i think I did pretty good, followed all the sterile procedures, but there'e always room for improvement.

I'm gonna store the last two brf paste jars in the fridge for now. I wanna see how these grain jars do before I make any other transfers.

I also have some spore prints from my last grow. I might go ahead and make another batch of brf jars and throw that on there and see if I get clean growth. Soo manny options right now!! :grin:



The key to sab work is to have a bleach soaked paper towels or towel on the bottom. I do like 50 50 bleach water. Then spray the walls of your sab including the roof with mostly dosh soap and just enough water to where you can spray it, but it doesn't drip. Any contams become stuck to walls for the most part and when they hit the bottom they are neutralized and you have a clean place to set your scalpel or what ever tools you may be using on. I got lazy with my most recent transfers and didn't even take my hands out the sab. I took a straight concentrated bleach paper towel and wiped my blade down in between each transfer and then set the scape down with the bleach rag around the blade covering it. Just make sure there isn't to much bleach on your scalpel when you cut into your plates and after you cut a sectio, try to stab it from the side and catch jt where the uncolonized media is under the mycelium on top. I did 10 ksss plates. I didn't get ONE comtam. Was kinda suprising but I might stick with that instead of going in and out and in and out of your sab. Also wipe down your grain jars with either bleach solution or isobefore putting them in the sab. Be quick because bleach fumes can be strong so try not to mix your bottom towel to loud.


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




Edited by PurePleasure (07/13/15 12:15 AM)


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21935813 - 07/13/15 12:57 AM (8 years, 6 months ago)

That sounds like a good approach to take, the contams from outside the SAB have less of a chance of getting inside if you don't take your hands out. Wouldn't making a glove box be the same thing tho? There's so much debate of whether a glove box vs an SAB is better. I always thought that the less airflow there is inside the box with/without gloves would give the best chance of no contams catching itself on exposed plates? :shrug:


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21935837 - 07/13/15 01:08 AM (8 years, 6 months ago)

I have pvc caps as my arm holes that have a screw on fitting for when ever you need to seal it.
I also took a sheet of thin rubber flexible gasket like material and glued it to the inside of my sab on the ends of both caps. The cut horizontal spits in them. So when you go in , you push through the slits and makes a pretty good sield around your long latex loves. I guess you could call it a hybrid :shrug:
Some may call it overboard, in just call it inenginuity
But yes the whole idea of a still air box is... well.. still air


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




Edited by PurePleasure (07/13/15 01:10 AM)


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21935843 - 07/13/15 01:12 AM (8 years, 6 months ago)

Sounds cool, I'll look into making those adjustments, I'm just hoping these grain jars do well! Only time will tell :smirk:


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OfflinePurePleasure
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21935858 - 07/13/15 01:22 AM (8 years, 6 months ago)

Ingot a feeling they will buddy just be quick with the cut, have your jar come out the pc still in foil and go straight to the sab if you don't wipe them down. Loosen your lids to where you can just pull it right off(one at a time as you go) , drop and seal your jar quick. . Leave it in the sab  until your done with ur jars and one there all done and closed, then take them out. Also after you bleach or flame your blade, have the agar or what ever dish loose so you can pull it off with one hand then just set the lid back on top after you cut.
If you drop a chunk on the bleach towel just forget about using it. So smoth, steady , and confident wins the race


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If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




Edited by PurePleasure (07/13/15 01:26 AM)


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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21935863 - 07/13/15 01:27 AM (8 years, 6 months ago)

Of and the rubber thing i did isn't necessary if you don't want to.


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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21936386 - 07/13/15 08:31 AM (8 years, 6 months ago)

Yeah I could be faster with cutting from the culture. And I need to make sure I don't hover around the exposed jars either. I'll get better with this in time. I'll definitely look into using the bleach/water spray. This time around, I used a soap/water spray throughout the SAB and wiped down each jar with iso before placing them inside. I also flamed the knife with a cigar lighter each time before making a cut. Hopefully my sterile procedures pay off tho, I hate seeing contams!! :cuss:


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21937210 - 07/13/15 12:54 PM (8 years, 6 months ago)

I wouldn't take sab advice from a guy who always has a crapton of bacteria in his grows :shrug:

bleaching and believing it "neutralizes" mold spores is just whack.
checkout how frank and TL does there sab prep and work.


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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
    #21937811 - 07/13/15 03:28 PM (8 years, 6 months ago)

I took a look at their teks, really good info for next time I use a SAB. Also I was gonna make a liquid innoculant with one of my brf paste jars. Once I inject the sterile water into the jar and move it around a bit, I'll suck up the mycelium water back into the syringe. Do you think I should store this syringe at room temp or should I leave it in the fridge until I'm ready to use it on grains or another jar?


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21937945 - 07/13/15 04:02 PM (8 years, 6 months ago)

Id refrigerate because of the BRF. Its already living mycellium so it doesn't need to expand. But before you use , id take it out and let it recover for 24 to 48 hours. I do the same when using a Lc. But thats just me and who knows why I do the things I do lol


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Re: Brf Substitute Tek Progress + Suggestions [Re: mycomaniac1402]
    #21937998 - 07/13/15 04:13 PM (8 years, 6 months ago)

I'll do that for sure, I've read about a lot of people doing the same thing. :thumbup:


Edited by Psilosoulful (07/13/15 04:14 PM)


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21938025 - 07/13/15 04:17 PM (8 years, 6 months ago)

I've said it before but I'll say it again you really should take a transfer first.


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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
    #21938287 - 07/13/15 05:18 PM (8 years, 6 months ago)

Quote:

spacechildo said:
I've said it before but I'll say it again you really should take a transfer first.



I will, I still have one more fully colonized jar left. I'm gonna pc more brf paste jars tonight and do some transfers tomorrow!! :dancer:


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21941984 - 07/14/15 01:01 PM (8 years, 6 months ago)

Made a nice mycelium filled syringe with one of the brf paste jars. Put it in the fridge just now. :grin:


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21942161 - 07/14/15 02:02 PM (8 years, 6 months ago)

Looks good man , that mycellium will kick into high gear and colonize quick. Ive made mycellium slurry but never BRF slurry. Was it puffy and lack linear growth? What im asking is was it more difficult to determine good growth on the paste because of the nutes rather than a agar media


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Re: Brf Substitute Tek Progress + Suggestions [Re: mycomaniac1402]
    #21942216 - 07/14/15 02:17 PM (8 years, 6 months ago)

It didn't isolate the dish, it grew from MS. I have one plate left that I'll use to isolate sections onto more brf jars. But, the mycelium was thick and fluffy all around the plate and there was rhizo growth all around the sides. I'm almost 100% positive it was clean spawn. Anyway, once I make some agar plates, I'll test it out to be sure it's contam free before knocking up more jars with the syringe. :thumbup:

I already have 7 WBS jars that I transferred a brf wedge to that had similar growth patterns. I'll take the best looking jar of the group, once 100% of course, and use it as a master grain jar. The rest I'll spawn to make my first full size mono :smile:

Now it's time to wait for some growth, I'm gonna have some beer to celebrate lol!! :hamsterdance:


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21946723 - 07/15/15 10:39 AM (8 years, 6 months ago)

Just took a peek in my fridge of the LI syringe I made yesterday, it turned dark blue!!!:ooo: I guess the mycelium was bruised from the process of making the LI or maybe I shook the syringe too hard. Has this ever happened to you guys? Looks cool I just hope it's still good. Should I still let it thaw out a bit before using it again or should I use it right away since there's bits of bruised mycelium in it that might contam at room temp, I'm not sure...


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21948755 - 07/15/15 06:23 PM (8 years, 6 months ago)

Did you put it close to the back of your fridge? Idk the only time I had a blue syringe from doing a syringe that way was when I put it the fridge under the power thing and it froze a bit. Oh but ya let it warm up for just a few minutes.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
    #21949755 - 07/15/15 10:55 PM (8 years, 6 months ago)

Quote:

DensePlacebo said:
Did you put it close to the back of your fridge? Idk the only time I had a blue syringe from doing a syringe that way was when I put it the fridge under the power thing and it froze a bit. Oh but ya let it warm up for just a few minutes.



I put it on one of the racks on the door. The temp should be relatively the same throughout, so I'm pretty sure it's bruised mycelium and not the temperature. I'm making more brf paste jars/grain jars tomorrow, so I'll be testing it out soon. I'm excited to see how this goes!! :mushdance:


Edited by Psilosoulful (07/15/15 11:47 PM)


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21953746 - 07/16/15 08:51 PM (8 years, 6 months ago)

Well then I'm sure your right. Keep us posted.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
    #21955979 - 07/17/15 12:41 PM (8 years, 6 months ago)

It was a bitch trying to transfer pieces of mycelium to other jars, I din't have an exacto knife or scalpel, so I used a sharp knife(didn't work out well). After failing to drop the pieces in the receiving jar because the knife was too big, I decided to use a syringe needle and that worked a lot better. I was able to get the pieces to the other jars but I ended up bruising the mycelium as I transferred it out. Hopefully it colonizes well with no contams.  :cookiemonster:


On another note, here are some WBS jars on day 4 of the original brf paste transfer. There's some nice rhizo growth going on!! :dancer:



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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21956186 - 07/17/15 01:30 PM (8 years, 6 months ago)

judgind by the pics your paste was clean! how big a piece did you put to grains?


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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
    #21956316 - 07/17/15 02:00 PM (8 years, 6 months ago)

I put a quarter sized piece of fully colonized brf paste in each grain jar, it seems to be doing well so far. I'm also planning on using the blue LI syringe I made earlier on some rye grains. I'll be posting pics of that soon. :grin:


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21957288 - 07/17/15 06:05 PM (8 years, 6 months ago)

Just curious, did you shake any after you dropped the wedges in?


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OfflinePsilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: Grey]
    #21957407 - 07/17/15 06:38 PM (8 years, 6 months ago)

Quote:

Grey said:
Just curious, did you shake any after you dropped the wedges in?



Yeah I shook each jar after dropping a small wedge in to redistribute it within the grains.


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21957608 - 07/17/15 07:33 PM (8 years, 6 months ago)

I don't shake when I drop my culture in. I do right before and leave it after I drop it then let it colonize 30-50 percent and then shake.


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If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21957797 - 07/17/15 08:14 PM (8 years, 6 months ago)

Quote:

PurePleasure said:
I don't shake when I drop my culture in. I do right before and leave it after I drop it then let it colonize



That's what I did, just shook it once, waiting for it to colonize right now. I'm gonna spawn to hpoo/verm/gypsum when it's 100% and use the last jar as a master grain. :laugh:




Edited by Psilosoulful (07/17/15 08:16 PM)


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21957809 - 07/17/15 08:17 PM (8 years, 6 months ago)

Glad everything is working out for you dude.


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If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21957902 - 07/17/15 08:34 PM (8 years, 6 months ago)

Thanks hopefully it goes well!! :awesome:


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
    #21960130 - 07/18/15 11:29 AM (8 years, 6 months ago)

With that good looking of growth I'm sure you will.


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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
    #21960210 - 07/18/15 11:49 AM (8 years, 6 months ago)

:nicesmile:


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