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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21946723 - 07/15/15 10:39 AM (8 years, 6 months ago) |
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Just took a peek in my fridge of the LI syringe I made yesterday, it turned dark blue!!! I guess the mycelium was bruised from the process of making the LI or maybe I shook the syringe too hard. Has this ever happened to you guys? Looks cool I just hope it's still good. Should I still let it thaw out a bit before using it again or should I use it right away since there's bits of bruised mycelium in it that might contam at room temp, I'm not sure...
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DensePlacebo
Stranger



Registered: 05/14/15
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21948755 - 07/15/15 06:23 PM (8 years, 6 months ago) |
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Did you put it close to the back of your fridge? Idk the only time I had a blue syringe from doing a syringe that way was when I put it the fridge under the power thing and it froze a bit. Oh but ya let it warm up for just a few minutes.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
#21949755 - 07/15/15 10:55 PM (8 years, 6 months ago) |
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Quote:
DensePlacebo said: Did you put it close to the back of your fridge? Idk the only time I had a blue syringe from doing a syringe that way was when I put it the fridge under the power thing and it froze a bit. Oh but ya let it warm up for just a few minutes.
I put it on one of the racks on the door. The temp should be relatively the same throughout, so I'm pretty sure it's bruised mycelium and not the temperature. I'm making more brf paste jars/grain jars tomorrow, so I'll be testing it out soon. I'm excited to see how this goes!!
Edited by Psilosoulful (07/15/15 11:47 PM)
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DensePlacebo
Stranger



Registered: 05/14/15
Posts: 186
Loc: canada
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21953746 - 07/16/15 08:51 PM (8 years, 6 months ago) |
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Well then I'm sure your right. Keep us posted.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
#21955979 - 07/17/15 12:41 PM (8 years, 6 months ago) |
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It was a bitch trying to transfer pieces of mycelium to other jars, I din't have an exacto knife or scalpel, so I used a sharp knife(didn't work out well). After failing to drop the pieces in the receiving jar because the knife was too big, I decided to use a syringe needle and that worked a lot better. I was able to get the pieces to the other jars but I ended up bruising the mycelium as I transferred it out. Hopefully it colonizes well with no contams.

 On another note, here are some WBS jars on day 4 of the original brf paste transfer. There's some nice rhizo growth going on!! 

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spacechildo
proletarians rise up



Registered: 01/24/13
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Loc: Babylon
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21956186 - 07/17/15 01:30 PM (8 years, 6 months ago) |
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judgind by the pics your paste was clean! how big a piece did you put to grains?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
#21956316 - 07/17/15 02:00 PM (8 years, 6 months ago) |
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I put a quarter sized piece of fully colonized brf paste in each grain jar, it seems to be doing well so far. I'm also planning on using the blue LI syringe I made earlier on some rye grains. I'll be posting pics of that soon.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21957288 - 07/17/15 06:05 PM (8 years, 6 months ago) |
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Just curious, did you shake any after you dropped the wedges in?
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: Grey]
#21957407 - 07/17/15 06:38 PM (8 years, 6 months ago) |
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Quote:
Grey said: Just curious, did you shake any after you dropped the wedges in?
Yeah I shook each jar after dropping a small wedge in to redistribute it within the grains.
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PurePleasure
Untrusted bacteria cultivator.


Registered: 08/29/14
Posts: 5,893
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21957608 - 07/17/15 07:33 PM (8 years, 6 months ago) |
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I don't shake when I drop my culture in. I do right before and leave it after I drop it then let it colonize 30-50 percent and then shake.
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21957797 - 07/17/15 08:14 PM (8 years, 6 months ago) |
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Quote:
PurePleasure said: I don't shake when I drop my culture in. I do right before and leave it after I drop it then let it colonize
That's what I did, just shook it once, waiting for it to colonize right now. I'm gonna spawn to hpoo/verm/gypsum when it's 100% and use the last jar as a master grain. 
Edited by Psilosoulful (07/17/15 08:16 PM)
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PurePleasure
Untrusted bacteria cultivator.


Registered: 08/29/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21957809 - 07/17/15 08:17 PM (8 years, 6 months ago) |
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Glad everything is working out for you dude.
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21957902 - 07/17/15 08:34 PM (8 years, 6 months ago) |
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Thanks hopefully it goes well!!
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PurePleasure
Untrusted bacteria cultivator.


Registered: 08/29/14
Posts: 5,893
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21960130 - 07/18/15 11:29 AM (8 years, 6 months ago) |
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With that good looking of growth I'm sure you will.
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21960210 - 07/18/15 11:49 AM (8 years, 6 months ago) |
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