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Psilosoulful

Registered: 09/05/14
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Brf Substitute Tek Progress + Suggestions
#21926612 - 07/10/15 11:10 PM (8 years, 6 months ago) |
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Watsup Mush Cult Viewers!! I'm very excited to restart my grow since early June. I had to take a short break due to lack of funds I'm back( currently soaking a good amount of WBS to sterilize tomorrow night!!) and have these brf paste jars that have been sitting around for about two weeks.
They are all fully colonized(i hope the paste isn't too thick). The mycelium is digging into the substrate and seems to have a good hold on it. I was wondering if it would be a good idea to make some liquid inoculant with the fluffy-like mycelium, transfer small sections of rhizo-like growth to more brf paste jars, or transfer sections to grain jars that I'm prepping at the moment?
I was reading about isolating sections of good mycelial growth before moving it to grain jars, but that would take a long time before a pure isolate is revealed. Anyway, I have three of these brf paste jars that look rather healthy and don't want them to go to waste.
I will be starting agar shortly(via Pastywhyte's tek) and want to see what I can do with these jars. I know a lot of growers don't like the agar substitute tek, but it's all I have at the moment. Thanks for the help! 

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DensePlacebo
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21926717 - 07/10/15 11:54 PM (8 years, 6 months ago) |
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I'm done all three with moderate success. I personally would wait for agar before I transferred onto brf again but that's not saying you couldn't do that. It only takes a little myc sooooo with three you could probably do all of the above. BTW once I found the right amount of agar to use in pastywhytes tek(I had alot of condensation, just me tho) I love that tek and I've used it for months now.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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PurePleasure
Untrusted bacteria cultivator.


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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
#21927352 - 07/11/15 03:56 AM (8 years, 6 months ago) |
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Wouldn't honey and water liquid cultures be cheaper then this paste concauction? Agar to lc, ftw
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21927590 - 07/11/15 06:25 AM (8 years, 6 months ago) |
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You could do limited multispore and just transfer a small section from the fastest growth on the plate.that'll narrow down the diversity a little.
You could put them to grain too. I've never done the brf substitute, but have had success with corn meal mush
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Psilosoulful

Registered: 09/05/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: DensePlacebo]
#21927807 - 07/11/15 08:00 AM (8 years, 6 months ago) |
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Quote:
DensePlacebo said: I'm done all three with moderate success. I personally would wait for agar before I transferred onto brf again but that's not saying you couldn't do that. It only takes a little myc sooooo with three you could probably do all of the above. BTW once I found the right amount of agar to use in pastywhytes tek(I had alot of condensation, just me tho) I love that tek and I've used it for months now.
Yeah i might just try doing all three methods and see which one works best. Hopefully this project goes well, I'm also gonna make a new SAB today! Then PC my grains tonight and do some work in the mornin'! 
Quote:
PurePleasure said: Wouldn't honey and water liquid cultures be cheaper then this paste concauction? Agar to lc, ftw
Never tried that out, but I had tons of brf just sitting around so I figured, why not make some paste jars! Can't wait to get into agar, I'm getting the telephone brand agar powder, is this a good type to use? I've read that some agar contains added sugars and this might interfere with solid growth. Just wanna make sure I got what I need to get started on the right foot.
Quote:
Grey said: You could do limited multispore and just transfer a small section from the fastest growth on the plate.that'll narrow down the diversity a little.
You could put them to grain too. I've never done the brf substitute, but have had success with corn meal mush
I'll try that method, do you think I'll be able to see some sectoring after the first transfer, or does that usually happen after multiple transfers? Because right now all I see is fluffy mycelium all around the plates, no rhizo growth yet.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21927818 - 07/11/15 08:04 AM (8 years, 6 months ago) |
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I don't mean to jack the thread but can someone please tell me once and for all if BRF+verm deceases potency like some people claim? is manure or compost really a better alternative for potency related issues? or are all substrates the same
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21927829 - 07/11/15 08:10 AM (8 years, 6 months ago) |
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Telephone Brand Agar agar is great.

Seeing sectoring usually takes at least a few transfers for me.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Brf Substitute Tek Progress + Suggestions [Re: Bill_Oreilly]
#21929412 - 07/11/15 03:49 PM (8 years, 6 months ago) |
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Quote:
Bill_Oreilly said: I don't mean to jack the thread but can someone please tell me once and for all if BRF+verm deceases potency like some people claim? is manure or compost really a better alternative for potency related issues? or are all substrates the same
Manure is the ideal substrate to work with, haven't tried it yet but I will be pasteurizing some horse manure when my grain jars are 100% colonized.
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ZeroBoyWD
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21929649 - 07/11/15 04:39 PM (8 years, 6 months ago) |
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I've done BRF cakes that were the fucking bomb. I didn't get as many flushes or a lot of weight but those cakes were SPENT on what I had. Depends on your conditions really
-------------------- I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.
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PurePleasure
Untrusted bacteria cultivator.


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Re: Brf Substitute Tek Progress + Suggestions [Re: Bill_Oreilly] 1
#21931664 - 07/12/15 12:36 AM (8 years, 6 months ago) |
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Quote:
Bill_Oreilly said: I don't mean to jack the thread but can someone please tell me once and for all if BRF+verm deceases potency like some people claim? is manure or compost really a better alternative for potency related issues? or are all substrates the same
Not really. Some might thrive better on other subs but the potency falls back on mostly genetics
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Psilosoulful

Registered: 09/05/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21932435 - 07/12/15 08:04 AM (8 years, 6 months ago) |
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UPDATE: Just took some WBS jars out of the PC i had running last night. I'm gonna wait for some of that excess moisture to evaporate before transferring a wedge of my brf jar to it. How do they look so far? I also used a piece of tyvek(kite-making material) over a 3/8" GE hole. In the meantime, it's time to construct my new SAB from a 66 qt sterilite tub using a coffee can. Ahh I love the smell of burning plastic in the mornin' 

Edited by Psilosoulful (07/12/15 08:10 AM)
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PurePleasure
Untrusted bacteria cultivator.


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21932489 - 07/12/15 08:30 AM (8 years, 6 months ago) |
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Those look rather wet. Shake them and wait a day if they still look like then i wouldn't even try it. And typically tyvek goes on the outside of your lid. Not nessecarry but still. That doesn't even look like tyvek... also did you cover the lids with foil so the tyvek wouldn't get wet? If not that's your problem. That's probably why they are way to wet and once tyvek gets soaked it's not really a good filter anymore because it opens the pores on it up even more.
Shake them really good and lay the jars on the side that way the water doesn't pool up in the bottom and become a breeding ground for a bacteria. After you shake them, hold the jars in your hand, flip them side ways and hold them up and look at the "bottom" of the side. Now roll it. If you see water tumbling through the grains on the bottom as it turns, probably to moist. The last pic looks wet, the others look borderline saveable. After the shake, shake it again 12 hr later and then when you wake up tom do what I stated above. Turn it on its side and see if water stays on the bottom as you turn the grains. If so the only thing I could think of is run them in your pc again but only for like 30 45 minutes at 15 psi. but cover those lids with foil!
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

Edited by PurePleasure (07/12/15 08:36 AM)
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Psilosoulful

Registered: 09/05/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21932546 - 07/12/15 08:52 AM (8 years, 6 months ago) |
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Thanks for the tips! I did cover the jar lids with foil but they did get kinda wet cuz when I took the foil off there was water droplets sitting on the tyvek. I thought that tyvek was waterproof tho? I'll be switching to SFD's soon, so I won't have this moisture problem. I've read that SFD's were the best to use.
I'll try that method you described and check back later. Hopefully, the grains dry up a bit. BTW I PC'd them for the standard 90 min at 15 psi. Link for the tyvek I used: http://www.amazon.com/Kitemaking-Material-Durable-Lightweight-Breathable/dp/B00G28H9QW/ref=sr_1_1?ie=UTF8&qid=1436712643&sr=8-1&keywords=tyvek
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PurePleasure
Untrusted bacteria cultivator.


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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21932618 - 07/12/15 09:20 AM (8 years, 6 months ago) |
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Quote:
Psilosoulful said: Thanks for the tips! I did cover the jar lids with foil but they did get kinda wet cuz when I took the foil off there was water droplets sitting on the tyvek. I thought that tyvek was waterproof tho? I'll be switching to SFD's soon, so I won't have this moisture problem. I've read that SFD's were the best to use.
I'll try that method you described and check back later. Hopefully, the grains dry up a bit. BTW I PC'd them for the standard 90 min at 15 psi. Link for the tyvek I used: http://www.amazon.com/Kitemaking-Material-Durable-Lightweight-Breathable/dp/B00G28H9QW/ref=sr_1_1?ie=UTF8&qid=1436712643&sr=8-1&keywords=tyvek
A little moisture on the tyvek isn't going to ruin it. I just think putting all that tyvek on the inside was a bad idea because unless you shake it right it's going to get wet with grain water and all the humidity in the bottles.BUT you will probably be fine. Tyvek really isn't water proof. It is to a certain extent. Its meant to be used a moisture barrier on houses but yet let it breathe and vent a little. But once it's Pc'd, it expands the pours a bit more. But that tyvek you used looks thick. But yes, sfds are amazing. I still use foil over them to keep from getting soaked even though sfds can deal with alot of moisture. If you want, I'll send you a disk. You can make like 8 lids with one sfd using a nickle and a sharpy and cut the outside of the mark to its a tad bigger. I drill a quarter inch holes in my lids, put red rtv silicone around the hole (a thin but steady smear around the hole, you don't want to have to much because when you push the sfd down the silicone can get under the lid and block up air flow on the other side a little bit.) Then when you push the sfd down go around the edges as if you were caulking it with some more silicone just to insure those bad boys are blocked the fuck off. Walmart has plastic lids, I think it's a 8 or 9 pack for like 3 or 4 bucks? I forget. If your interested in that , pm me.
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Psilosoulful

Registered: 09/05/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: PurePleasure]
#21932646 - 07/12/15 09:36 AM (8 years, 6 months ago) |
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Thanks man!! The tyvek is actually outside the lid. I siliconed it on and put some extra on the outside to be on the safe side. I've always ran into contam issues when using filters on the underside of jars. Not sure why vendors recommend putting SFD's under the lid tho?
I also got a pack of 12 SFD's coming in the mail, should be here by Monday! Wanted to get a head start on these brf paste jars, since they are at full colonization already.
I'll definitely look into getting plastic jar lids, I think I saw them for a decent price last time I went to WalMart. I'll buy a pack for my next grain jars. These metal jar lids are gonna be a bitch to open up inside a SAB. I tightened the lids before PC'ing so the vacuum inside the jar is gonna be strong. I'll update once I make the transfers.
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Psilosoulful

Registered: 09/05/14
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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21934317 - 07/12/15 04:54 PM (8 years, 6 months ago) |
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My WBS jars dried up nicely after distributing the moisture in the jar by shaking them vigorously for like 2 minutes!! Time to do some transfers 


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spacechildo
proletarians rise up



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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21934327 - 07/12/15 04:57 PM (8 years, 6 months ago) |
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nicely prepped!
have you done any tranfers of myc from brf sub to brf sub? your first pic looks off to me (bacteria), try not to have brf paste all over the jar too next time.
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Psilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
#21934413 - 07/12/15 05:20 PM (8 years, 6 months ago) |
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Quote:
spacechildo said: nicely prepped!
have you done any tranfers of myc from brf sub to brf sub? your first pic looks off to me (bacteria), try not to have brf paste all over the jar too next time.
I haven't done any transfers yet, still gotta make a SAB, I have a 66qt sterilite tub that I'm gonna use. Yeah, that first jar looks kinda funky, I'll make sure I transfer sections from the other two healthy brf jars.
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spacechildo
proletarians rise up



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Re: Brf Substitute Tek Progress + Suggestions [Re: Psilosoulful]
#21934431 - 07/12/15 05:26 PM (8 years, 6 months ago) |
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I'm not 100% but is all your brf paste covered in myc? you wanna do a transfer as soon as you see some myc germination to try and beat the contam spores from germinating.
if they grew all out just try to take a tiny piece and see what grows.
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Psilosoulful

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Re: Brf Substitute Tek Progress + Suggestions [Re: spacechildo]
#21934568 - 07/12/15 06:14 PM (8 years, 6 months ago) |
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They are all 100% colonized and the mycelium is climbing up the sides. I'll be transferring some sections out tonight. I have 7 WBS jars that I'm gonna use. These are the three brf paste jars I'm using. I'm still debating whether or not to use the jar in the last pic, looks a bit off to me.


Edited by Psilosoulful (07/12/15 06:15 PM)
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