Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Mono Tub Substrate   Left Coast Kratom Buy Kratom Extract   North Spore Boomr Bag

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
question about incubation / phase after inoculation
    #21925340 - 07/10/15 04:44 PM (8 years, 6 months ago)

Hey guys, after I inoculated the jars, do I need to leave them sealed, covered with aluminum foil or with the lid exposed with a hole and the filter? .

I think my biggest question is : does the mycelium need gas exchange (c02/02) after they are put in a jar or they need to be isolated to they don't get contamined?

Thanks,
Daniel


Extras: Filter Print Post Top
OfflineThunderbird310
Caps


Registered: 02/19/14
Posts: 187
Last seen: 4 years, 7 months
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21925375 - 07/10/15 04:54 PM (8 years, 6 months ago)

The foil is there so water doesn't drip into your jar during pressure cooking. If you are using a synthetic filter disk, are using poly fill or if you have self healing injection ports, remove the foil.


Extras: Filter Print Post Top
OfflineOPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
Re: question about incubation / phase after inoculation [Re: Thunderbird310]
    #21925399 - 07/10/15 05:04 PM (8 years, 6 months ago)

Are you doing PF? If so you can leave it or remove it. If you leave it it might be more likely to stall. If you take it off there's a higher chance of contams although it should not be by much if you did your dry verm layer correctly.


--------------------
"Wasn't that fun"


Extras: Filter Print Post Top
OfflineIkarus
Whooooshhh

Folding@home Statistics
Registered: 06/13/14
Posts: 61
Last seen: 6 years, 5 months
Re: question about incubation / phase after inoculation [Re: OPB]
    #21925484 - 07/10/15 05:28 PM (8 years, 6 months ago)

No matter which method you are using, take the foil off. It needs to breath, plus I would speculate that the foil could trap moisture and create a bacteria problem.


Extras: Filter Print Post Top
OfflineDarkhome
•Shaman•N•Training•
Male User Gallery


Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: question about incubation / phase after inoculation [Re: Ikarus]
    #21925512 - 07/10/15 05:38 PM (8 years, 6 months ago)

I'm still new, so take it for what it's worth... It's my understanding that in PF tek... You should take off the foil, If you are using Verm or a filter, you should be fine. They need to breathe.:crazy2:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Edited by Darkhome (07/10/15 06:07 PM)


Extras: Filter Print Post Top
InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: question about incubation / phase after inoculation [Re: Darkhome]
    #21925527 - 07/10/15 05:42 PM (8 years, 6 months ago)

Leave the inoc ports uncovered for gas exchange. They need gas exchange or else it will slow the growth by a lot. Also you don't need to incubate the jars, just put them on a shelf and don't fuck with them too much.

I had this same question about whether or not you need to have the ports covered. My response is the same response many trusted cultivators gave me. So use the advice. :smile:


--------------------


Edited by Dr.Satan (07/10/15 05:50 PM)


Extras: Filter Print Post Top
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
Re: question about incubation / phase after inoculation [Re: Dr.Satan]
    #21925572 - 07/10/15 05:57 PM (8 years, 6 months ago)

It's being stored/ incubated in the SAB box which I carefully hygenized with alchohol and lysol using gloves, the bottles were also hygenized from outside and the box is closed


Extras: Filter Print Post Top
InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21925586 - 07/10/15 06:04 PM (8 years, 6 months ago)

Quote:

hiperhiper said:
It's being stored/ incubated in the SAB box which I carefully hygenized with alchohol and lysol using gloves, the bottles were also hygenized from outside and the box is closed




Totally unnecessary. I keep mine in a tote that has the lid off just so I can move them all at once when I need too. The dry vermiculite layer you put on top serves as a contamination filter. So in other words as long as your not fucking with the jars making that layer move around you have nothing to worry about. The thing that gave me contam problems in my jars was I left the tape on for the first 5 or 6 days and it kept in too much moisture which caused mold/bacteria growth issues.


--------------------


Edited by Dr.Satan (07/10/15 06:04 PM)


Extras: Filter Print Post Top
OfflineDarkhome
•Shaman•N•Training•
Male User Gallery


Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: question about incubation / phase after inoculation [Re: Dr.Satan]
    #21925609 - 07/10/15 06:12 PM (8 years, 6 months ago)

:whathesaid:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Extras: Filter Print Post Top
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
Re: question about incubation / phase after inoculation [Re: Darkhome]
    #21925837 - 07/10/15 07:34 PM (8 years, 6 months ago)

I just checked the jars and they are mostly half colonized.
Do I shake them?

some pics:











Another question.

I have coco coir and frozen cooked grains and I have plans to do a bulk method. Do I still have a big contamination risk after these jars are colonized and placed in the fruiting chamber? any suggestions on the method to use?

Thanks,
Daniel


Extras: Filter Print Post Top
InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21925851 - 07/10/15 07:39 PM (8 years, 6 months ago)

Oh you're using grains. Well once the jar is fully colonized the odds of contamination are greatly decreased.


--------------------


Extras: Filter Print Post Top
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
Re: question about incubation / phase after inoculation [Re: Dr.Satan]
    #21925878 - 07/10/15 07:48 PM (8 years, 6 months ago)

even if you add more substrate when moving to the big container? or I'm not supposed to do that and just add coco coir?


Extras: Filter Print Post Top
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21926020 - 07/10/15 08:26 PM (8 years, 6 months ago)

I think I might be confused about this.
I'm not supposed to add extra substrate (fungus food) after the jar process, but just casing (coco coir, vermiculate) correct?


Extras: Filter Print Post Top
InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21926280 - 07/10/15 09:30 PM (8 years, 6 months ago)

If you add more substrate then you must wait for it to colonize the new substrate.


--------------------


Extras: Filter Print Post Top
OfflineThunderbird310
Caps


Registered: 02/19/14
Posts: 187
Last seen: 4 years, 7 months
Re: question about incubation / phase after inoculation [Re: Dr.Satan]
    #21926850 - 07/11/15 12:53 AM (8 years, 6 months ago)

Am I seeing things or is there vermiculite in those jars?


Extras: Filter Print Post Top
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
Re: question about incubation / phase after inoculation [Re: Thunderbird310]
    #21927181 - 07/11/15 02:41 AM (8 years, 6 months ago)

vermiculate


Extras: Filter Print Post Top
InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: question about incubation / phase after inoculation [Re: Thunderbird310]
    #21927620 - 07/11/15 06:41 AM (8 years, 6 months ago)

Quote:

Thunderbird310 said:
Am I seeing things or is there vermiculite in those jars?



I don't see vermiculite in those jars, it looks like some sort of grains or something to me :shrug: if it is verm then he got some real chunky shit lol


--------------------


Extras: Filter Print Post Top
Offlinehiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
Re: question about incubation / phase after inoculation [Re: Dr.Satan]
    #21928411 - 07/11/15 11:17 AM (8 years, 6 months ago)

I mixed them, maybe I was not supposed to do that right?


Extras: Filter Print Post Top
InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21928512 - 07/11/15 11:43 AM (8 years, 6 months ago)

I've never heard of mixing grains with verm. Not to say that it's a bad idea to do so, but I've only ever seen people inoc jars that were only grains or only wbs as far as I know they didn't mix anything with them. Hopefully a grower more experienced with grains can weigh in on this. And if you got the mixing info from a tek on here then it's probably correct.


--------------------


Extras: Filter Print Post Top
OfflineThunderbird310
Caps


Registered: 02/19/14
Posts: 187
Last seen: 4 years, 7 months
Re: question about incubation / phase after inoculation [Re: Dr.Satan]
    #21928566 - 07/11/15 11:52 AM (8 years, 6 months ago)

I think verm could be beneficial actually, but it may make the grains difficult to get out of the jar. I've never seen any trusted tek that talks about mixing grains with verm. You might be ok. If you haven't already, shake the jars and then maybe do it one more time before 100%. If the grains and verm form a solid mass then you may have to break the jar to get it out? Looks healthy otherwise.


Extras: Filter Print Post Top
OfflineFreeWorldOrder
Male User Gallery

Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA Flag
Last seen: 8 days, 6 hours
Re: question about incubation / phase after inoculation [Re: Thunderbird310]
    #21928762 - 07/11/15 12:49 PM (8 years, 6 months ago)

Shake well at around 20%. Then let I would let them finish colonizing. After they are 100% colonized some people will shake once again to see how the grains recover. They usually recover in a couple days. If they take more than a few days then there is a possibility they are contaminated with bacteria. (A lot of times a bacterial contamination will still fruit if it doesn't stall or get another type of contam in it's weakened state)

This info is from personal experience. Others may have different opinions...  :thumbup:


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: question about incubation / phase after inoculation [Re: hiperhiper]
    #21928916 - 07/11/15 01:38 PM (8 years, 6 months ago)

Quote:

hiperhiper said:
I think I might be confused about this.
I'm not supposed to add extra substrate (fungus food) after the jar process, but just casing (coco coir, vermiculate) correct?




yes, once the grains in the jar is completely colonized (100& white) you mix them in a tray/monotub with substrate (mushroom food).
then you let the grains and substrate colonize to 100% in the tray or monotub.

once its all white you can choose to add a casing layer (not meant to be food). coir and verm works well both as substrate and casing material.

The verm in grain jars is an old way of ensuring your grains arent to wet.
its better to prep the grains right and leave the verm out :thumbup:

Franks 12 step to mushrooms


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: question about incubation / phase after inoculation [Re: spacechildo]
    #21929109 - 07/11/15 02:31 PM (8 years, 6 months ago)

Wow. If you've been in the archives stay away from there. You don't want any sort of incubation. Germination colonization and fruiting is best in room temperature. Other than that just listen to the rest of the members


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineThunderbird310
Caps


Registered: 02/19/14
Posts: 187
Last seen: 4 years, 7 months
Re: question about incubation / phase after inoculation [Re: Mad Season]
    #21931410 - 07/11/15 11:31 PM (8 years, 6 months ago)

Also, to answer your other question, after your grains are fully colonized(verm or no verm) you will want to mix it with a bulk substrate or case the grains. Either way you can use a 50/50 mix of coir/verm (there are plenty of other options including horse poo, peat moss straw mixes). It is absolutely vital that you pasturize (but not sterilize) the bulk substrate or casing material before you mix with the spawn. Mix the spawn (your colonized grains) and bulk substrate (pasturized verm and coir, or whatever you choose) and leave them to colonize. If using bulk you can go right into fruiting without a casing layer, or you can case the grains and then put them into fruiting. In dry climates casing seems to help. Frank has a really easy to follow grain tek that I recommend for beginners. My best advise is to make a glovebox and buy a pressure cooker if you want good results. Hope you find a method that suits you. Also look into monotubs. They are the future.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Mono Tub Substrate   Left Coast Kratom Buy Kratom Extract   North Spore Boomr Bag


Similar ThreadsPosterViewsRepliesLast post
* First timer with Invitro/Neglect Tek Questions mtglu 3,350 7 09/12/23 09:52 AM
by DERRAYLD
* Quick casing question Ska_mad_hatter 997 8 12/02/02 10:43 AM
by Viveka
* who uses lights in there incubator?ANNO? nw2shroom 2,154 6 05/30/02 03:04 AM
by GreyMatter
* Too much light while incubating? BleaK 677 2 10/28/02 09:04 PM
by BleaK
* Another Newbie Question about casing's? La_casper 3,214 12 06/25/02 11:07 AM
by nw2shroom
* Countdown to Mushies, last minute questions sillvyr 883 4 01/19/02 03:15 AM
by AssHumper10K
* Re: After Steralization - how much time before enoculation and other questions? Lizard King 1,507 4 12/25/99 08:48 AM
by Gadget
* Cat Litter(Bentonite) as a Vermiculate Substitution barisk 2,522 8 06/20/05 10:25 AM
by pcubmycol

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,779 topic views. 38 members, 267 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.011 seconds on 14 queries.