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InvisibleKauaiOrca
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Registered: 08/12/08
Posts: 3,131
Violet's V-Tek ???
    #21924287 - 07/10/15 12:33 PM (8 years, 6 months ago)

Does anyone use or have an opinion on the extensive tek Violet posted using RGS and ziploc containers?  It seems like one of the simplest systems of all.  One substrate, minimal chance of contams, easy system to clone and find suitable genetics and stealthy as heck. 

I'm just wondering if anyone has tried it and what you're experience is with RGS in those ziploc plastic quarts used as the fruiting chamber?  Any success?  Any strains that do better than others in that grow environment?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Invisible36fuckin5
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21924304 - 07/10/15 12:41 PM (8 years, 6 months ago)

All the people in Violet's thread have an opinion. Ask there.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21924308 - 07/10/15 12:41 PM (8 years, 6 months ago)

I don't have much of an opinion but here is an experiment by pasty, comparing v-tek to bulk.

http://www.shroomery.org/forums/showflat.php/Number/19219682


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Offlinespacechildo
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Re: Violet's V-Tek ??? [Re: insanemike]
    #21924776 - 07/10/15 02:00 PM (8 years, 6 months ago)

oh man, that thread. see ya in a week OP! :lol:


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InvisiblebodhisattaMDiscordReddit
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Re: Violet's V-Tek ??? [Re: spacechildo]
    #21924922 - 07/10/15 02:46 PM (8 years, 6 months ago)

haha, lots of hype, not a lot of results to back them up, not many cultivators, who I consider good cultivators, have been able to meet the claims including myself.


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OfflineMachiavelliavore
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Re: Violet's V-Tek ??? [Re: KauaiOrca] * 1
    #21925022 - 07/10/15 03:24 PM (8 years, 6 months ago)

I've done a fair amount of experimentation with it.  Bottomwatering works wonderfully and it produces dense, beautiful, well proportioned mushrooms.  I learned on it and it tought me a lot about how to work with grains and do casings.  Would recommend.

I cannot comment on invitro quarts yet, as I have not used them.  An invitro glass quart I had did alright, but underyielded and was prone to aborts.  Dunno why.  It was a crapshoot that I paid little attention to.  If it works like she says, it should be great.  About to test some invitro pp5 quarts with PF cakes, so that should give me a good idea.  IMO probably worth using a casing even if you opt for grass seed, as my v-tek invitro half pints side-pin HARD.

I used standard V-tek with pint containers, which requires a fruiting chamber.  I do not have a greenhouse yet, and other fruiting chambers I've used have not functioned too well. In short, an unmodified underbed tote created a boss pinset but oxygen starved them during fruiting, a shotgun minitub dried them out, and a std 60qt tote only fit 6 or 8 of my square containers so I never even bothered to try.

My best yield was around 9oz wet (just checked my notes) off 2 containers, 1 cup rgs each, multispore.  I think it would have been better with a good FC and a better pinset.  Doesn't really compare to the 20oz+ wet I've gotten off 3 cups of WBS spawned to 1.5qt coir.  RGS is much less dense than WBS though.

Personally, I intend to use Vtek almost exclusively once my greenhouse is up.  I just really like the fruits.  Bottomwatering is pretty easy: when you see the pins are getting to the point where they explode in volume, just pour some water in to compensate for what's about to be used.  Grass seed was an easy grain to start with, but now I'm really digging rice.  Even though it's easy to burst rice, it strains to the point where it's not wet on the outside and ready to PC in a matter of minutes.  If I can hit 7.5oz per container as she says she's done with ideal cultures, it will be on par with bulk yields.

IMO, the problem with V-tek is that there is no good smedium way to do it like mini-monos.  Sounds like invitro may have solved that problem.

P.S.
*Check dollarstores for pp5 containers.  $3 for 2 twist n locks is pretty spendy.  I've paid 1$ for a pair nearly the same.
*WBS works well for this tek also.  I think it may have lower water capacity (not much space for mycellium between grains) and require a bit more monitoring for bottomwatering.
*PP5 twisttop quarts are what I would want for grain spawn for bulk anyway, so there is literally NOTHING lost by trying invitro vtek.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (07/10/15 03:42 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21925078 - 07/10/15 03:43 PM (8 years, 6 months ago)



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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21925085 - 07/10/15 03:45 PM (8 years, 6 months ago)

If you are into pp5 containers, muda's tek is the shit. Everything stays sterile until fruiting and you can put pretty much anything in them because of that. It kind of brings v-tek and bulk together.
anyway, here is the link.
http://www.shroomery.org/forums/showflat.php/Number/21168958


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InvisibleKauaiOrca
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Re: Violet's V-Tek ??? [Re: insanemike]
    #21925347 - 07/10/15 04:46 PM (8 years, 6 months ago)

Quote:

insanemike said:
If you are into pp5 containers, muda's tek is the shit. Everything stays sterile until fruiting and you can put pretty much anything in them because of that. It kind of brings v-tek and bulk together.
anyway, here is the link.
http://www.shroomery.org/forums/showflat.php/Number/21168958




I think his tek is awesome, still requires a fruiting chamber which is what intrigues me about  the latest version of Violet's RGS tek where you only fill the pp5 container about 1/3 of the way and use the headroom for fruiting.  That's the one I'm wondering about in terms of success.  It has no casing, no mixing of substrate, no exposure to contams and no fruiting chamber necessary … pretty amazing if it works as  you just prepare your grains, PC, inoculate and wait.  Bottom water when it pins … that's about as simple as it gets.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21925396 - 07/10/15 05:04 PM (8 years, 6 months ago)

Quote:

KauaiOrca said:
Quote:

insanemike said:
If you are into pp5 containers, muda's tek is the shit. Everything stays sterile until fruiting and you can put pretty much anything in them because of that. It kind of brings v-tek and bulk together.
anyway, here is the link.
http://www.shroomery.org/forums/showflat.php/Number/21168958




I think his tek is awesome, still requires a fruiting chamber which is what intrigues me about  the latest version of Violet's RGS tek where you only fill the pp5 container about 1/3 of the way and use the headroom for fruiting.  That's the one I'm wondering about in terms of success.  It has no casing, no mixing of substrate, no exposure to contams and no fruiting chamber necessary … pretty amazing if it works as  you just prepare your grains, PC, inoculate and wait.  Bottom water when it pins … that's about as simple as it gets.




Give me a couple weeks, I'm working on something I like to call a singleshot fruiting chamber which uses those quart pp5's. I'm in the process of developing the inlet/outlet configuration. I should have the hole configuration figured out by the end of the weekend. I will be writing a tek.


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OfflineMushroom_J
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21925455 - 07/10/15 05:21 PM (8 years, 6 months ago)

I did brf cakes instead of rgs.
Multispore. 4 containers.
It's over all cheaper and same results as cakes in in a sgfc.
Don't need a fruiting chamber. I've averaged about a 1/2 oz off each container so far. . I think that's pretty good.

Some flushes were better than others.










--------------------


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InvisibleKauaiOrca
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Posts: 3,131
Re: Violet's V-Tek ??? [Re: Mushroom_J]
    #21925638 - 07/10/15 06:23 PM (8 years, 6 months ago)

Quote:

Mushroom_J said:
I did brf cakes instead of rgs.
Multispore. 4 containers.
It's over all cheaper and same results as cakes in in a sgfc.
Don't need a fruiting chamber. I've averaged about a 1/2 oz off each container so far. . I think that's pretty good.

Some flushes were better than others.













That's awesome and exactly what I was looking for!  Did you have to case the BRF cakes?  Did you just crack the lids a bit as she suggests to get them to fruit and your pictures look like the fruits are bigger than the PP5 quart jars so did you have to take the lids off and just let them grow out when they got big?

Great pics and thanks for the response!!!


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisibleInocuole
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Re: Violet's V-Tek ??? [Re: Mushroom_J]
    #21925772 - 07/10/15 07:12 PM (8 years, 6 months ago)

Quote:

Mushroom_J said:
I did brf cakes instead of rgs.
Multispore. 4 containers.
It's over all cheaper and same results as cakes in in a sgfc.
Don't need a fruiting chamber. I've averaged about a 1/2 oz off each container so far. . I think that's pretty good.

Some flushes were better than others.













Dude I'm sorry but all that verm all over everything, what the fuck?  All on the scale, all on the caps, all on the table, probably on the floor.  Sometimes I think I'm the only one that cleans up my shit before I weigh it.  Ain't nobody interested in how much verm weighs.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisibleblindingleaf
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Re: Violet's V-Tek ??? [Re: Inocuole]
    #21925931 - 07/10/15 08:04 PM (8 years, 6 months ago)

i bet it weighs less than the peat i leave on mine :rofl2:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleInocuole
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Re: Violet's V-Tek ??? [Re: blindingleaf] * 1
    #21926141 - 07/10/15 08:57 PM (8 years, 6 months ago)

I'm sure that's tasty.  But nah I give everybody shit about that,  I'm also super meticulous about getting the fruits 100% clean before they hit the dehydrator or the scale.  Some people's harvests be lookin like a caveman just ripped them up out of there with fists.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleGrey
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21926396 - 07/10/15 10:05 PM (8 years, 6 months ago)

Quote:

KauaiOrca said:
Quote:

Mushroom_J said:
I did brf cakes instead of rgs.
Multispore. 4 containers.
It's over all cheaper and same results as cakes in in a sgfc.
Don't need a fruiting chamber. I've averaged about a 1/2 oz off each container so far. . I think that's pretty good.

Some flushes were better than others.













That's awesome and exactly what I was looking for!  Did you have to case the BRF cakes?  Did you just crack the lids a bit as she suggests to get them to fruit and your pictures look like the fruits are bigger than the PP5 quart jars so did you have to take the lids off and just let them grow out when they got big?

Great pics and thanks for the response!!!




PP5 quart and a PP5 kids cup.


It works fine doing it the way she wrote it up. You can case, but you'll still get mushrooms of you don't. I got an eighth dry first flush from the PP5 quart. If your going for bulk, do Muda's bottles.:awesomenod:


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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InvisibleSteveRogers
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21926422 - 07/10/15 10:11 PM (8 years, 6 months ago)

Does anyone use or have an opinion on the extensive tek Violet posted using RGS and ziploc containers?

Yes I have an opinion. It is an extremely inefficient waste of time, energy, and resources. She makes incredible claims that can only be replicated by her under dubious circumstances. Shit science if I've ever seen it.


--------------------
"General, I am loyal to nothing......except The Dream"


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InvisiblePastywhyteMDiscord
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Re: Violet's V-Tek ??? [Re: Grey] * 1
    #21926425 - 07/10/15 10:12 PM (8 years, 6 months ago)

Invitro is all well and good but has its drawbacks. I find that the fruits are often lanky from high CO2 and will need extra headroom. I basicly just rest the lid on top so they can push it off. This is counterproductive if you are doing it for really clean prints but works fine if you're just growing small amounts for fun.


This sub was straw and flour.



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InvisiblePastywhyteMDiscord
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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21926443 - 07/10/15 10:18 PM (8 years, 6 months ago)

I am still playing around with V tek but I find like any method it has ups and downs. I have yet to find a cube culture it really likes but there is real potential for exotics. Muda did some sweet pans in it and my pans liked it as well. I am gonna keep at it for cubes and I plan to try a few edibles with it as well.

I do dislike bottom watering, I can barely be bothered to mist tubs sometimes, preferring to case and forget. I also greatly dislike the cost of RGS which is really high for Canadians. I am experimenting with other grains all the time. Millet is next :smirk:


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InvisibleViolet
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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21926578 - 07/10/15 11:00 PM (8 years, 6 months ago)

I like all of the methods, and get results exactly like what's described in the threads! :grin::thumbup:


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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InvisibleSteveRogers
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Re: Violet's V-Tek ??? [Re: Violet]
    #21926584 - 07/10/15 11:01 PM (8 years, 6 months ago)

:goodluckwiththat2:


--------------------
"General, I am loyal to nothing......except The Dream"


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InvisibleViolet
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Re: Violet's V-Tek ??? [Re: SteveRogers]
    #21926610 - 07/10/15 11:09 PM (8 years, 6 months ago)

:trollersgonnatroll:


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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InvisiblePastywhyteMDiscord
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Re: Violet's V-Tek ??? [Re: Violet]
    #21926616 - 07/10/15 11:11 PM (8 years, 6 months ago)

Hey Violet good to see you :wave:


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InvisibleViolet
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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21926630 - 07/10/15 11:16 PM (8 years, 6 months ago)

You too, Pasty! I'll message you sometime to catch up, when it's not so late! I've been pretty much completely gone from here for months, barely peeking in and checking PMs. Get with ya soon!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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InvisiblePastywhyteMDiscord
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Re: Violet's V-Tek ??? [Re: Violet]
    #21926637 - 07/10/15 11:20 PM (8 years, 6 months ago)

Cool :thumbup:


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OfflineMachiavelliavore
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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21927190 - 07/11/15 02:42 AM (8 years, 6 months ago)

I have no doubt that bottomwatering works.  IMO grain subs are also easier to prepare then PF subs.  Grass seed may not have significantly higher yield potential for the same volume because it's light.  Also, if violate's postulate that cubes like to pin off low nutrition areas is correct, a cased grain with have a much better chance of only pinning off the casing than a PF sub invitro.  IMO it would be worthwhile using a casing in invitro to get a better pinning surface, even though strictly speaking it isn't necessary.  That also opens you up to using any grain you want with greater success.

I honestly think this tek is so controversial mostly because it goes against male thinking.  i.e. Want a bunch of mushrooms?  Well, we start with a dump truck of coir and a cement mixer full of spawn, then pour them together in a swimming pool.
Take a container that wouldn't hold your lunch, then fill it half full of grass seed and water it like a flowaaaaa, and you should let it grow doesn't really sound as intuitively compelling. 

Having gotten 4.5oz/cup off multispore grass seed, (nearly what she says you can get off grass seed,) so I think it's quite compelling that an ideal culture in an ideal FC on rice or wbs could hit 7.5oz, given the density difference.
If 4 containers in my greenhouse to be would take up about 2/3 of the space of one of my foil pans, use no coir, require only the same preparation as a spawn grain, use 1/4 the quantity of casing, and produce equal quantities of better fruits, I find it hard to argue against that.  They are a bit higher maintainence though.

I've had both hollow and dense fruits come off coir, but these v-tek fruits didn't give when squeezed at all, and their colouration indicated that that might be, ahm, very :mushroom2:flavourful:mushroom2:  Seemed a bit more cap happy with a thinner stem than coir fruits.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleKauaiOrca
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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21927710 - 07/11/15 07:29 AM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
I have no doubt that bottomwatering works.  IMO grain subs are also easier to prepare then PF subs.  Grass seed may not have significantly higher yield potential for the same volume because it's light.  Also, if violate's postulate that cubes like to pin off low nutrition areas is correct, a cased grain with have a much better chance of only pinning off the casing than a PF sub invitro.  IMO it would be worthwhile using a casing in invitro to get a better pinning surface, even though strictly speaking it isn't necessary.  That also opens you up to using any grain you want with greater success.

I honestly think this tek is so controversial mostly because it goes against male thinking.  i.e. Want a bunch of mushrooms?  Well, we start with a dump truck of coir and a cement mixer full of spawn, then pour them together in a swimming pool.
Take a container that wouldn't hold your lunch, then fill it half full of grass seed and water it like a flowaaaaa, and you should let it grow doesn't really sound as intuitively compelling. 

Having gotten 4.5oz/cup off multispore grass seed, (nearly what she says you can get off grass seed,) so I think it's quite compelling that an ideal culture in an ideal FC on rice or wbs could hit 7.5oz, given the density difference.
If 4 containers in my greenhouse to be would take up about 2/3 of the space of one of my foil pans, use no coir, require only the same preparation as a spawn grain, use 1/4 the quantity of casing, and produce equal quantities of better fruits, I find it hard to argue against that.  They are a bit higher maintainence though.

I've had both hollow and dense fruits come off coir, but these v-tek fruits didn't give when squeezed at all, and their colouration indicated that that might be, ahm, very :mushroom2:flavourful:mushroom2:  Seemed a bit more cap happy with a thinner stem than coir fruits.




Thanks!  Super helpful post.  I'm going to try it!


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisibleKauaiOrca
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Re: Violet's V-Tek ??? [Re: Violet]
    #21927723 - 07/11/15 07:34 AM (8 years, 6 months ago)

Quote:

Violet said:
I like all of the methods, and get results exactly like what's described in the threads! :grin::thumbup:




Hi Violet - Thanks a million for the great write-up on your tek!  I'm going to try it.  Absolutely love how between the two sized jars can create virtually everything.  Agar, multi spore starters to use for LI, fruiting right in the jars, clean, no mixing, one ingredient and super stealthy. 

I think it's one of the most creative and simple methods out there and your write up is absolutely incredible. 

Did you find it makes any difference at all what strain you start off with as I don't have a ton of prints.  I have vendor syringes of PE, PE6, Texas, Amazon and South American … any thoughts at all from your experience?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21928020 - 07/11/15 09:10 AM (8 years, 6 months ago)

http://www.shroomery.org/forums/showflat.php/Number/19219682

:goodluckwiththat2:

There is a time and a place for V-Tek, good luck in your attempts.
By the way CAN WE STOP CALLING IT V-TEK AND JUST CALL IT FRUITING FROM PP5 CONTAINERS.

Quote:

RogerRabbit said:
Just a heads up; It's highly frowned upon in mycology to name any procedure or any mushroom or culture, etc., after oneself.  Teks should be named for what they are or what they accomplish.  :wink:

People have been hydrating and sterilizing grass seed for many years.  People have also been casing grass seed and other grains for many years. 

We always appreciate members posting their projects and giving info on how they do things, but re-wording something or using plastic or tin instead of glass doesn't make it a new tek.
RR




Quote:
RogerRabbit said:
You gotta be shitting me.  We don't grow mushrooms on Violet.  We grow mushrooms on substrates.  This crap about naming stuff after oneself is going to stop.  We have 50K members.  We can't have 50K teks named after each person, instead of what the procedure is actually doing.


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Edited by SteveRogers (07/11/15 09:23 AM)


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Re: Violet's V-Tek ??? [Re: SteveRogers]
    #21928065 - 07/11/15 09:22 AM (8 years, 6 months ago)

Quote:

SteveRogers said:
http://www.shroomery.org/forums/showflat.php/Number/19219682

:goodluckwiththat2:

There is a time and a place for V-Tek, good luck in your attempts.
By the way CAN WE STOP CALLING IT V-TEK AND JUST CALL IT FRUITING FROM PP5 CONTAINERS.




I have no idea why it would bother you what it is called?  I've read a lot of threads and hers is very unique the way she uses the two sizes of PP5 containers for agar, very small amount grain culturing, no casing of RGS, invitro grow in the containers and super clean prints.  The detail in her write-up is extraordinary.  If there is another tek or write-up that is identical to this, I never saw it.  It's not just fruiting from PP5 containers.  It's a complete system from start to finish of culturing, growing, printing all in a sterile environment.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21928083 - 07/11/15 09:26 AM (8 years, 6 months ago)

Read Pasty's log and get back to me. If you have time and money to burn then go V-Tek all day. There are much better ways to go about what she is attempting there.


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Re: Violet's V-Tek ??? [Re: SteveRogers]
    #21928137 - 07/11/15 09:40 AM (8 years, 6 months ago)

You all need to get your panties out of a bunch. You did not lose a member to the darkside. If this member chooses a method that not many agree with, it's their choice. So don't derail their thread because you don't aprove. Move on.


Edited by insanemike (07/11/15 09:43 AM)


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Re: Violet's V-Tek ??? [Re: SteveRogers]
    #21928162 - 07/11/15 09:47 AM (8 years, 6 months ago)

Indeed...

Funny how people like to cherry-pick stuff like that out when it suits their bias.
Note that, as I said to RR back in those days of his half-attentiveness, we don't grow mushrooms on ProfessorFanaticus either. Yet there seems to be no problem with the 1,000 daily references to PFtek.

People like you are just trying to start new flak and drama by pointing to the fact that, indeed, old drama did exist.
That, and a few mediocre or semi-failed attempts that were posted early on, somehow means to you that the court finds these methods "guilty".  But this requires covering one's eyes and saying "nananana na" to the fact that I have shown several times the results that I get, and others get the likes as well, such as Machi mentions himself in THIS VERY THREAD.

I wish more people would follow the leads of peeps like Pasty, Muda, Cron... Far better growers than any of the monkeys that have flung poo, by the way, and apparently smarter in general.

The grow tek works.  Like any tek does in a variety of circumstances and skills, it works differently for different people.
Just makes me wonder why this is all a personal problem to the likes of you.

Goosfraba.


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Re: Violet's V-Tek ??? [Re: Violet]
    #21928178 - 07/11/15 09:51 AM (8 years, 6 months ago)

Quote:

Violet said:

I wish more people would follow the leads of peeps like Pasty




Me too. He proved categorically that your tek was a waste of time, space resources, ect.

My only concern on this board is spreading useful efficient techniques. The BE of your tek is laughable. Period


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Re: Violet's V-Tek ??? [Re: SteveRogers]
    #21928182 - 07/11/15 09:54 AM (8 years, 6 months ago)

I'm pretty sure that he wouldn't even make that claim himself.  Careful about putting words in Pasty's mouth.

Oh, and a very blasphemous use of the word "proof" there.  You're bound for science hell if you don't turn your life around quick, sonny


I'm starting to wonder which old troll puppet you are with your 330 posts in 9 years...
Seems like your real only concern on this board is popping in to troll a very few specific topics.
My be is not "laughable" .... "Period" hahahah.... in the least bit.  It's better than yours to say the least ;P


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Re: Violet's V-Tek ??? [Re: Violet]
    #21928191 - 07/11/15 09:57 AM (8 years, 6 months ago)

Chill. your tek is valid and you should be proud. just not my cup of tea. Not trying to start anything here. i wish you success on your future grows. there is no need to insult me. I have been doing this longer than this site has existed. Just calm down.

for the record....Pasty's grows are something you should aspire to.

I stand by my statement your BE is sub par.


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Edited by SteveRogers (07/11/15 10:06 AM)


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Re: Violet's V-Tek ??? [Re: Violet]
    #21928205 - 07/11/15 10:04 AM (8 years, 6 months ago)

I think some people are mixing up v-tek and violets invitro bottle tek. I've never tried v-tek, and tbh probably won't. However, the invitro tek she has works. I don't do it all the time, but when I've got some leftover LI I make some up. It's fun because once it's inoculated I don't touch a thing until I harvest. It eliminates all the "how do my cakes look?" "Am I misting/fanning too much/too little?".

I still prefer mono's and muda bottles for bulk no doubt.

Chill guys.


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Re: Violet's V-Tek ??? [Re: Grey]
    #21928289 - 07/11/15 10:41 AM (8 years, 6 months ago)

V has a RSS feed any time someone like me says her name boom she's here.

There's plenty of other methods I find to be lack luster in comparison to available methods. I try to help people grow mushrooms  in my own way. The only vendeta aspect comes from the attitude V uses and they hype machine that goes along with the methods. No one else has a built in hype machine in their teks. Plain and simple anyone who claims potency increases simply because of the method rather than hard work with strain isolation is going to be called out by me. You can ignore me if it causes too much butthurt to listen to my critical assessment but I'm not going to stop saying when people ask just because V has a hard time with criticism of a sub par TEK. It would be a hell of a lot less chastised if it were not for the noob eye catching claims for things that only come from hard work not a re-hased method, like all current teks just being re-hased and improvement on old methods. Nothing here is clearly unique nothing. It's all based on what has already existed and constantly improving.


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21928320 - 07/11/15 10:50 AM (8 years, 6 months ago)

I was wondering about the rss feed. I just thought she was searching her name or something.


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Re: Violet's V-Tek ??? [Re: Grey]
    #21928333 - 07/11/15 10:55 AM (8 years, 6 months ago)

All I know is I'm opinionated I disagree with almost everyone on something or another. I say it like I see it. If you got a problem with me, ignore button please. I do what I do and I know there's plenty of helped out people to show for it.

I'm not putting anyone on my ignore list permanently just because they want to troll, but being called a troll myself is always a good laugh. I think I would have been found out by now from more than just one person if I was a little troll fuck....


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21928440 - 07/11/15 11:25 AM (8 years, 6 months ago)

What's a RSS feed?  I just pop in every once in a while. I do way more stuff with my life and I spare very little attention to here now. If it seems like I respond quickly, it's coincidence. I don't even look at the dates.  But this thread showed up on the forums with my name on it - Pasty's last post before mine was just like 40mins beforehand. Whatever an RSS feed is, one wouldn't need it for that shit, it's like a big neon sign sharing "THIS IS A RARE POST RELEVANT TO YOU"
But of course that doesn't sound nearly so catty against me, does it, so of course it's not the conclusion you jump to.

Uh Bod, you have openly admitted to trolling me. Remember? It was this whole big thing? So in your own words, you ARE a "troll fuck", just not like a shill or whatever else you think it means to be a troll.


See, I DONT work hard to get my results.  I USED to work hard to get results that were AT BEST similar to my AVERAGE results now.  Working long days making zany lids, preparing giant vats of grain, sterilizing and handling hot jars, breaking up growth, precisely hydrating substrates, dunking bags of it with bricks into temperature-controlled hot baths for hours on end, cleaning and setting up tubs... I have much better success just sterilizing an easy seed in an unmodified container, inoculating, and fruiting right from there.

So rather than getting my results with "hard work" and skill, I have actually become a LAZY grower, and do better than ever.  It's just working smart, not hard.

No matter how much time you spend being contrarian to me on an online forum, you can never magically invalidate my consistent experience with my own methods. So I will never stop saying that my methods have been easier and more productive for me than anything I've tried (which includes pretty much everything except Pasty's new jam)


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (07/11/15 03:47 PM)


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Re: Violet's V-Tek ??? [Re: Violet]
    #21928555 - 07/11/15 11:51 AM (8 years, 6 months ago)

Wow. There is no need for all this anamosity guys. We are as a community fortunate to have a host of methods and materials at our disposal. We have people willing to do the legwork to polish these methods and provide their findings for the benefit of all. Some people maybe are overly protective of their methods and have a hard time with criticism. I can understand that. It takes a fair amount of guts to post a write-up and put your name on it (or have your name attached to it by the community). I feel that any method that can actually produce fruits has at least some fundamentals that we can learn from. Whether they suit our personal needs is something else, but that doesn't mean there is no value or nothing to be learned.

Violet takes a fair amount of criticism here,  some of it is warrented, but much of it is directed at her due to the attacks made by anne on the people who prefer bulk. Since anne is banned,  violet takes it in her stead. Its in the past guys, lets leave it there please.

I have done just about every method there is to grow mushrooms. I have found merit in all and I have yet to find one that doesn't have at least one drawback. The only way we grow as cultivators is to open our minds up and put hubris aside.

At the moment I am in the position of having to give up bulk as I need to deal with grow area concerns and renovations (and some IRL stuff). In the meantime i will be only able to continue my work with the rustywhyte on a small scale which means invitro, bottles, and v tek. I am grateful that these small scale options exist allowing me to continue an area of my work while my issues surrounding infrastructure are resolved. All things have value. Lets not forget that.

I am going to be working on a non spawned method going forward as well. It shows great promise.  It also has kinks that I am still working on. But once I work them out, it will produce a BE that is incredible. This was actually inspired by some of violets methods even tho it doesn't really resemble them. We can all learn from each other.

This was 70 grams worth of substrate.



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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21928750 - 07/11/15 12:46 PM (8 years, 6 months ago)

Wow, as the OP I had no idea my question would inspire this kind of combat!  I really appreciate all of the interest and Violet, want to thank you for coming in and participating. 

Does anyone know if it's likely to get better yields using Muda's bottle tek but only filling the zip loc  quart jar 1/3 of the way like Violet suggests and just fruiting it in the jar with no fruiting chamber or would it be better to go with RGS and casing or no casing at all?  I'm mostly interested in fruiting invitro in the jar, no tub or sgfc for stealth reasons.  Just want to hear what people think would be the best formula to put in the jar for simplicity that taps into the bottom watering principle to increase yields?????????

That's the kind of questions I'd love to see discussed rather than analysis of poster's personalities or motivations.  I think we can all agree that Violet has written up some very thorough and super helpful information in a really giving way.  That deserves, at least in my humble opinion, a big thank you from the community.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21928974 - 07/11/15 01:50 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Wow. There is no need for all this anamosity guys. We are as a community fortunate to have a host of methods and materials at our disposal. We have people willing to do the legwork to polish these methods and provide their findings for the benefit of all. Some people maybe are overly protective of their methods and have a hard time with criticism. I can understand that. It takes a fair amount of guts to post a write-up and put your name on it (or have your name attached to it by the community). I feel that any method that can actually produce fruits has at least some fundamentals that we can learn from. Whether they suit our personal needs is something else, but that doesn't mean there is no value or nothing to be learned.

Violet takes a fair amount of criticism here,  some of it is warrented, but much of it is directed at her due to the attacks made by anne on the people who prefer bulk. Since anne is banned,  violet takes it in her stead. Its in the past guys, lets leave it there please.

I have done just about every method there is to grow mushrooms. I have found merit in all and I have yet to find one that doesn't have at least one drawback. The only way we grow as cultivators is to open our minds up and put hubris aside.

At the moment I am in the position of having to give up bulk as I need to deal with grow area concerns and renovations (and some IRL stuff). In the meantime i will be only able to continue my work with the rustywhyte on a small scale which means invitro, bottles, and v tek. I am grateful that these small scale options exist allowing me to continue an area of my work while my issues surrounding infrastructure are resolved. All things have value. Lets not forget that.

I am going to be working on a non spawned method going forward as well. It shows great promise.  It also has kinks that I am still working on. But once I work them out, it will produce a BE that is incredible. This was actually inspired by some of violets methods even tho it doesn't really resemble them. We can all learn from each other.

This was 70 grams worth of substrate.






:whathesaid: to add to that, we should put in effort to become stronger as community by supporting each other instead of bashing on each other. The only way progress can continue is if we open our minds and apply what we learn to further along our own know how. Violet opened up a whole new world of possibities with the pp5 containers. The same pp5 containers that muda swears by. if not for violet tek, maybe muda wouldn't have expanded upon the idea of converting it to his own needs as in sterile bulk substrates. I love it and I support any progress being made.


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Re: Violet's V-Tek ??? [Re: insanemike]
    #21929011 - 07/11/15 02:02 PM (8 years, 6 months ago)

Quote:

insanemike said:


:whathesaid: to add to that, we should put in effort to become stronger as community by supporting each other instead of bashing on each other. The only way progress can continue is if we open our minds and apply what we learn to further along our own know how. Violet opened up a whole new world of possibities with the pp5 containers. The same pp5 containers that muda swears by. if not for violet tek, maybe muda wouldn't have expanded upon the idea of converting it to his own needs as in sterile bulk substrates. I love it and I support any progress being made.




Really well said, Mike.  If you remember, when you come out with your new ideas on the tek you said you're working on, I hope I see it as I'm really interested. If you want to PM me on it, I'd love to know more.  I'm going to commit to this style of growing for a number of reasons, I could care less if the yield is a bit better with a bulk grow because It just doesn't work for me to have big tubs all over the place.  I like the ability to test a lot of different strains and cultures, do small grows, have it all be very stealthy and no need for growing chambers.  What I'm looking for is the best possible substrate with no casing needed to grow completely invitro in the PP5 jars with no fruiting chamber needed.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21929402 - 07/11/15 03:46 PM (8 years, 6 months ago)

Grain mixes with bulk substrates are very effective for invitro grows like those! No need for casing layers since the low-nutrient bulk is all over, and they have a water reserve on hand so are less needy for water, though you will still need to add water.


--------------------
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PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Violet's V-Tek ??? [Re: Violet]
    #21929455 - 07/11/15 04:00 PM (8 years, 6 months ago)

Quote:

Violet said:
and they have a water reserve on hand so are less needy for water, though you will still need to add water.




Very true, this one sucked so much water and nutes out that the sub was being pulled up from the bottom of the bottle. Only got 2 flushes from it cause it was so spent.



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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21929602 - 07/11/15 04:28 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Quote:

Violet said:
and they have a water reserve on hand so are less needy for water, though you will still need to add water.




Very true, this one sucked so much water and nutes out that the sub was being pulled up from the bottom of the bottle. Only got 2 flushes from it cause it was so spent.






My God what a flush!  What kind of substrate and fruiting chamber did you use to get that kind of results?  Would love to find a culture that would grow like that!


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21929666 - 07/11/15 04:42 PM (8 years, 6 months ago)

Substrate was oats, coir, verm, straw, hpoo, gypsum, and worm castings. Fruiting chamber was a monotub with holes stuffed and filled with other bottles. Culture was a strong clone.


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21929668 - 07/11/15 04:42 PM (8 years, 6 months ago)

Quote:

KauaiOrca said:
Quote:

Pastywhyte said:
Quote:

Violet said:
and they have a water reserve on hand so are less needy for water, though you will still need to add water.




Very true, this one sucked so much water and nutes out that the sub was being pulled up from the bottom of the bottle. Only got 2 flushes from it cause it was so spent.






My God what a flush!  What kind of substrate and fruiting chamber did you use to get that kind of results?  Would love to find a culture that would grow like that!





Quote:

bodhisatta said:
http://www.shroomery.org/forums/showflat.php/Number/20591316
and
http://www.shroomery.org/forums/showflat.php/Number/21168958




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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929701 - 07/11/15 04:50 PM (8 years, 6 months ago)

as for cloning too it doesn't have to be made that hard either

cloning and culturing is easier without the convoluted methods of thinking you have to keep everything in-vitro and sterile.

strain isolation, agar work, and cloning are incredibly easy tasks with some practice. playing ship in a bottle is great if you can't manage to figure those techniques out.

some of the v methods give noobs this idea that these techniques are somehow difficult and doing everything in such a sterile way is somehow an easier more effective way of doing things. this is noob appeal 101.


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929737 - 07/11/15 04:57 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
as for cloning too it doesn't have to be made that hard either

cloning and culturing is easier without the convoluted methods of thinking you have to keep everything in-vitro and sterile.

strain isolation, agar work, and cloning are incredibly easy tasks with some practice. playing ship in a bottle is great if you can't manage to figure those techniques out.

some of the v methods give noobs this idea that these techniques are somehow difficult and doing everything in such a sterile way is somehow an easier more effective way of doing things. this is noob appeal 101.




I guess I was reacting more to the fast and easy way to test a clone or culture out on a small cake of RGS right in a fruiting chamber … super fast and easy.  You could test out a bunch of them simultaneously without needing a lot of different fruiting chambers going on, etc.  I'm not much of a fan of the PF Tek method, SGFC, dunking, rolling, misting fanning, etc … One step system from picking a clear grain/pin and seeing if it fruits in the middle of the small RGS cake and taking it from there.  Seems like less transfers but thanks for the advice and I'm trying the MUDA bottles right now with 3 new strains … hopefully with good results but too early to tell.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21929764 - 07/11/15 05:02 PM (8 years, 6 months ago)

take your clones from a grow just the same thing you plan on doing your full sized grows otherwise your clone isn't representative of the conditions and or substrate you're trying to optimize.

you should test clones in a very similar setup to your full sized ambitions too.
test cakes are fine though if you want to make quick and easy testing of clones just make PF cakes in shot glasses or 1/4 pints and use your clone culture on those


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929842 - 07/11/15 05:26 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
take your clones from a grow just the same thing you plan on doing your full sized grows otherwise your clone isn't representative of the conditions and or substrate you're trying to optimize.

you should test clones in a very similar setup to your full sized ambitions too.
test cakes are fine though if you want to make quick and easy testing of clones just make PF cakes in shot glasses or 1/4 pints and use your clone culture on those




How do you then grow out your small 1/4 pint cakes?  In a SGFC?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929846 - 07/11/15 05:27 PM (8 years, 6 months ago)

Give it a rest for once man. Christ.  You seem to be assuming Orca is highly ignorant about alternatives, and couldn't possibly have made this choice on purpose.

The culture method doesn't make cloning hard.  It makes it WAY easier than other alternatives where you have to take some kind of sterile biopsy.  Tweeting a pin from one container to another could not POSSIBLY be easier.  Duh...

Yeah, growing your brf sub in an invitro container is WAY simpler, easier, and more assured than PFtek. I'd like to see a well-founded argument with *significant* points to the contrary.  "Noob appeal 101" is apparently a pretty damn good suggestion then. Thanks.
Although perhaps you're just cutting at the higher sterile success rates with my method.  Also a good thing, and also verifiably true.  I just don't see where you get off pretending these things are somehow sensationalist.  They're obvious.  I end how I began - give it a rest for once.


--------------------
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21929853 - 07/11/15 05:28 PM (8 years, 6 months ago)

I usually just do things open air with a vermiculite casing, put a ziploc bag or a grocery bag over the top with some holes while you're gone.

or you could shove them in some monotub real estate if you do the monotub thing and are going to do the the monotub thing for your jars.


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Re: Violet's V-Tek ??? [Re: Violet]
    #21929855 - 07/11/15 05:30 PM (8 years, 6 months ago)

Quote:

Violet said:
Give it a rest for once man. Christ.  You seem to be assuming Orca is highly ignorant about alternatives, and couldn't possibly have made this choice on purpose.

Yeah, growing your brf sub in an invitro container is WAY simpler, easier, and more assured than PFtek. I'd like to see a well-founded argument with *significant* points to the contrary.  "Noob appeal 101" is apparently a pretty damn good suggestion then. Thanks.
Although perhaps you're just cutting at the higher sterile success rates with my method.  Also a good thing, and also verifiably true.  I just don't see where you get off pretending these things are somehow sensationalist.  They're obvious.  I end how I began - give it a rest for once.



you're ignored until you disappear for another few months, like I said if you don't like my opinions buzz off or put me on ignore. I don't give any fucks about your feels OK got it?


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929876 - 07/11/15 05:34 PM (8 years, 6 months ago)

Yay!


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929880 - 07/11/15 05:35 PM (8 years, 6 months ago)

anyways...



if you want to make a test cake, or even just make a cake to get a mushroom to clone it's super easy and fast

it literally takes 10m to make the cake mix your water verm brf put into a jar put your lid on(I used a grain jar lid and do these cakes semi in-vitro)

inoculate with a syringe or agar or whatever else.

there's no grain prep and the sterilization time on a cake this small in a pressure cooker is minimal, or you can just use a steam bath with a much much bigger pot since steam baths work for PF cakes and you don't need a PC.

either way keep it easy, quick, simple, fast, cheap, etc..


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21929916 - 07/11/15 05:45 PM (8 years, 6 months ago)

... And you're not telling him anything different than my invitro gs/brf tek. :thumbup:


--------------------
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PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
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Re: Violet's V-Tek ??? [Re: Violet] * 1
    #21933954 - 07/12/15 03:22 PM (8 years, 6 months ago)

Quote:

Violet said:
I like all of the methods, and get results exactly like what's described in the threads! :grin::thumbup:




So, Violet, after reading through a bunch of your write-ups it seems you have gotten the best yields in the invitro PP5 quart jars with brown rice grains (not PF cakes) and a casing layer, right?  I'm not talking about culturing, but growing out once you've got a good clone/culture?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: Violet]
    #21946217 - 07/15/15 08:00 AM (8 years, 6 months ago)

Quote:

Violet said:
I like all of the methods, and get results exactly like what's described in the threads! :grin::thumbup:




Violet, have you ever tried fruiting sclerotia (galindoi) invitro in the bottles after you harvested stones?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21946476 - 07/15/15 09:11 AM (8 years, 6 months ago)

I did Galindoi recently. It colonized and just sat there forming stones for two months. I loosened the lid and waited a couple more weeks before casing it. It consumed the casing and didn't do anything. It ended up triching out the other day at the three month Mark.


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Re: Violet's V-Tek ??? [Re: Grey]
    #21961961 - 07/18/15 07:23 PM (8 years, 6 months ago)

I think Galindoi, perhaps other truffle species too, may do best made to fruit ASAP after colonization.  I mean that particularly to a tek like this, but it may even be best that way in general.  I had meant to try fruiting Galindoi in regular containers as per the normal tek both with stones as well as freshly colonized, to see if they'd still perform as well after so long forming stones. Idunno.  But for the invitro tek, I suspect that chances are a lot better if you case and fruit right at full colonization. And don't be afraid to crack that lid extra loose!


--------------------
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21962077 - 07/18/15 07:47 PM (8 years, 6 months ago)

Quote:

KauaiOrca said:
Quote:

Machiavelliavore said:
I have no doubt that bottomwatering works.  IMO grain subs are also easier to prepare then PF subs.  Grass seed may not have significantly higher yield potential for the same volume because it's light.  Also, if violate's postulate that cubes like to pin off low nutrition areas is correct, a cased grain with have a much better chance of only pinning off the casing than a PF sub invitro.  IMO it would be worthwhile using a casing in invitro to get a better pinning surface, even though strictly speaking it isn't necessary.  That also opens you up to using any grain you want with greater success.

I honestly think this tek is so controversial mostly because it goes against male thinking.  i.e. Want a bunch of mushrooms?  Well, we start with a dump truck of coir and a cement mixer full of spawn, then pour them together in a swimming pool.
Take a container that wouldn't hold your lunch, then fill it half full of grass seed and water it like a flowaaaaa, and you should let it grow doesn't really sound as intuitively compelling. 

Having gotten 4.5oz/cup off multispore grass seed, (nearly what she says you can get off grass seed,) so I think it's quite compelling that an ideal culture in an ideal FC on rice or wbs could hit 7.5oz, given the density difference.
If 4 containers in my greenhouse to be would take up about 2/3 of the space of one of my foil pans, use no coir, require only the same preparation as a spawn grain, use 1/4 the quantity of casing, and produce equal quantities of better fruits, I find it hard to argue against that.  They are a bit higher maintainence though.

I've had both hollow and dense fruits come off coir, but these v-tek fruits didn't give when squeezed at all, and their colouration indicated that that might be, ahm, very :mushroom2:flavourful:mushroom2:  Seemed a bit more cap happy with a thinner stem than coir fruits.




Thanks!  Super helpful post.  I'm going to try it!




^^do it! Tried cakes at first when I started and violet's V-Tek got me more flushes, bigger fruits, and less contams. The tek is easy to follow for a newer grower like myself. I used WBS instead of grass and just watered a bit more for the same results. It was amazing how simple it made growing after I had freaked out and gotten over involved with cakes o the point of fucking them up almost. I 100% recommend it.

Violet, your tek has been super helpful for me and has allowed me to get individual clones for genetics easily. I produced my biggest fruit ever off of WBS with your tek, nothing giant giant, but a 6g dry fruit made me super happy. For the lack of effort I put in the results were great, fruitng coincided with the beginning of my last year of grad school so time was and issue, but apparently not with your tek. I did 2liters on top of them for individual chambers and all four containers went 3 flushes at least with 2 going 5.

Great tek in this noobs opinion!


-MIM


--------------------
“the only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.”

I surround myself with people who aren't afraid to live. They have had what they love most taken from them, be it freedom, love, money, or anything for that matter. When you lose your greatest love you also lose your greatest fear, when that happens you are completely and utterly free.


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Re: Violet's V-Tek ??? [Re: MagicInMichigan]
    #21962168 - 07/18/15 08:07 PM (8 years, 6 months ago)

^With WBS, I think there's less volume available between the grains, meaning less mycellium at full colonization.

This, I believe, means that the WBS sub has less water capacity than other subs, and thus it's more critical to hit your bottom-watering timings correctly, since there is less buffer capacity to compensate for human error.  I suspect this is where the conventional wisdom of "don't use WBS as a cased grain" comes from.  Without the mid flush watering it will just deplete too quickly.

That said, it really doesn't seem like it's hard to get it right, even with WBS.  Once you've grown a bit and have a sense of when the pins expand massively in volume, you just pre-empt that.  I like to picture the mushrooms growing up being made of pure water.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21962291 - 07/18/15 08:34 PM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
^With WBS, I think there's less volume available between the grains, meaning less mycellium at full colonization.

This, I believe, means that the WBS sub has less water capacity than other subs, and thus it's more critical to hit your bottom-watering timings correctly, since there is less buffer capacity to compensate for human error.  I suspect this is where the conventional wisdom of "don't use WBS as a cased grain" comes from.  Without the mid flush watering it will just deplete too quickly.

That said, it really doesn't seem like it's hard to get it right, even with WBS.  Once you've grown a bit and have a sense of when the pins expand massively in volume, you just pre-empt that.  I like to picture the mushrooms growing up being made of pure water.





Exactly. WBS wasn't ideal, but with a little extra watering it worked out fine. I'm planning on trying it with grass seed here for my next grow once my agar isolates are ready. If grass seed hold moisture better and makes it easier than it already does with WBS, then damn I don't think I'll have one bad thing to say about her tek, except maybe that its too easy and every lazy high on(LIKE ME! LOL.) would grow their own mushrooms so I'd stop being able to trade for all my other psychedelic needs. My main fear is my DMT friend doing this and eliminating my free supply. :smile:


-MIM


--------------------
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I surround myself with people who aren't afraid to live. They have had what they love most taken from them, be it freedom, love, money, or anything for that matter. When you lose your greatest love you also lose your greatest fear, when that happens you are completely and utterly free.


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Re: Violet's V-Tek ??? [Re: MagicInMichigan]
    #21962409 - 07/18/15 09:00 PM (8 years, 6 months ago)

Whole brown rice has been a joy to work with and prep.  The cool thing about WBR over WBS or GS is that it dries out almost imediately.

Most of the water strains off within a few minutes, and the rest evaporates within 15, especailly if you spread it out.  It is extremely easy to burst.  I prefer 10 minutes of attention to prevent bursting rather than leaving drying grains out for hours.

It's often like 70c/lbs at bargain markets and dollstore in small convenient packaging.

1 lbs of WBR dumped into 9qt boiling water and returned to a boil starts to burst in about 8 minutes for me.  For a 2qt saucepan 1 lbs prep, violets 2-day short boil prep nailed the hydration.  It seems her prep doesn't account for changing heat diffusion in different quantities of water, and is more accurate for small <4qt quantities IME.  A large stock pot will stay near boiling temps for quite some time.  Be curious what she has to say.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21962621 - 07/18/15 10:14 PM (8 years, 6 months ago)

inoculated my first 4 V-tek quart jars of RGS with PE about 3 days ago and growth is solid.  Looking forward to really seeing this grow completely invitro.  Want to get some good pins/clones to find one of the super productive cultures.


--------------------
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21963128 - 07/19/15 12:30 AM (8 years, 6 months ago)

PE really likes a casing from what I've read, as does this tek.  Consider using one.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21963595 - 07/19/15 06:23 AM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
PE really likes a casing from what I've read, as does this tek.  Consider using one.




thanks for advice.  The way I'm approaching it is in 2 ways:

1) Using RGS and no casing for complete invitro growing to get the cleanest pins/clones and find a great culture.  This keeps the growing chamber (PP5 Quart jar) as contam free as possible.  Also,with the smaller PP5's, it can be used to generate really clean LI by just adding water to the colonized grain and sucking it back out.
2) Once some good cultures come from #1, then moving to WBR and casing to get the better fruits as the primary way of growing them out.

Just hoping to find one great culture that really likes this growing method.  I love how easy it can be, but I'm so early in the process I have no results to celebrate yet.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Edited by KauaiOrca (07/19/15 06:44 AM)


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21963959 - 07/19/15 09:28 AM (8 years, 6 months ago)

You need to case grains,  even if the microclimate at the surface was perfect a casing would still be needed to provide a decent pinning surface. While I won't say its impossible for them to pin from grain, a casing really helps.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21963978 - 07/19/15 09:33 AM (8 years, 6 months ago)

Quote:

Pastywhyte said:
You need to case grains,  even if the microclimate at the surface was perfect a casing would still be needed to provide a decent pinning surface. While I won't say its impossible for them to pin from grain, a casing really helps.




P - What do you think of Violet's invitro CULTURING technique with RGS and no casing?  I guess the point is to keep the invitro jars clean while culturing/finding a good clone then switch to WBR + casing when you want bigger yields?

Do you think that's a good strategy?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21963988 - 07/19/15 09:36 AM (8 years, 6 months ago)

I prefer to clone my clones from something more similar to the conditions Im planning on growing in. An in vitro pinning on straight rgs or grain clone isn't the same as a clone from how you plan on doing your real grows. There's no reason to be so stupidly concerned with how clean your clone is. If you cant clone without having to do it all in vitro then don't bother just get better at sterile techniques


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21964031 - 07/19/15 09:51 AM (8 years, 6 months ago)

I didn't see you were doing it just to find clones. I'm not familiar with the method so I won't really say anything more other than there is no reason it can't work.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21964045 - 07/19/15 09:58 AM (8 years, 6 months ago)

Pasty, invitro grass seed pins excellently right from the grain! It's the only one I've seen do so, and I'm pretty sure it's due to the small size and quick consumption of the grain, especially considering how little nutrition-per-myc there is. But it def has to be invitro, or the surface dries out, akin to what you said.


Bod, the reasons to be "so stupidly concerned" with taking clones sterile in this fashion are
1) you can take PINS, young and healthy with little maturity and ready to grow back out nearly instantly as thick healthy running myc, and
2) you can take a dozen clones with the ultimate ease of a tweezer in minutes, with 100% clean success pretty much guaranteed.

It eliminates the tedium of cloning and increases the success, eleminating the necessity for skills that only exist to deal with work-hard-not-smart methodology.

Besides, I have never seen a clone taken with my culture method perform any less spectacularly in any grow method it's applied to.


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Re: Violet's V-Tek ??? [Re: Violet]
    #21964132 - 07/19/15 10:11 AM (8 years, 6 months ago)

I usually just make my agar a little richer and it almost always pins from ms. 90% of the clones that I have kept long term came from pins off my agar. Then I transfer to a slant and a plate. The plate is used to test the genetics, the slant is kept based on the outcome. Its not hard but I guess it does neccessitate having access to test tubes.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21964225 - 07/19/15 10:34 AM (8 years, 6 months ago)

I wonder if Bod hounds your related posts about not taking Your clones in conditions like the intended final grow. :tongue: My bet is no.

That is a good way too. What I like about mine is that it is indeed a normal fruiting substrate.  IME pins aren't as likely to indicate their full-fruit potential on agar.  My agar pins are mostly numerous but tiny with a high "abort" rate - as in, staying large knots or small pins, even from cultures with medium or larger fruits.  I think it has a bit more indication of what type of genes are being selected - clusters, potential size, speed on fruiting sub, that sort of things.  But your way hits some of the important parts of mine, too, i.e. minimal expansion.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21964404 - 07/19/15 10:58 AM (8 years, 6 months ago)

Quote:

Pastywhyte said:
I usually just make my agar a little richer and it almost always pins from ms.



Have you ever used gypsum in your agar? I was reading an old RR post and I think he was saying gypsum in agar caused more pins.

-found it

Quote:

RogerRabbit said:
I just use the agar from fungi.com but mix it up a bit weaker than called for in the recipe.  I also add a pinch or two of gypsum to the litre of agar, and this seems to help fruiting.  A bit of gypsum in jars helps invitro fruits to form also by the way.  My experience with mexicana is it's all about strain isolation.  Some substrains just won't make sclerotia, and others won't fruit.  By growing out on agar you can find the best substrains.  The chunk of sclerotia below was sliced into three pieces and each one was transferred to a new dish.  These were grown out and transferred to grains as the fruiting strain.  The best sclerotia producers were likewise selected and grown out on rye grain, which in my experience is superior to rye grass seed for sclerotia production.
RR




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Re: Violet's V-Tek ??? [Re: blackout]
    #21964445 - 07/19/15 11:05 AM (8 years, 6 months ago)

Quote:

blackout said:
Quote:

Pastywhyte said:
I usually just make my agar a little richer and it almost always pins from ms.



Have you ever used gypsum in your agar? I was reading an old RR post and I think he was saying gypsum in agar caused more pins.




Well when I make gw agar there is gypsum in it from the soak. I do find gw agar pins quite easy so maybe that is the reason. Tests will need to be done.

Violet, bodhi and I do not agree on all things and have had debates many times. I think the difference is that for me I don't care if I am proven wrong, I see it as a learning experience. My opinion is that cultures that can pin in horrible conditions with nearly no food and no FAE are gonna be aggressive no matter where they end up. Most of the time in practice that bears out. If anyone has solid evidence to the contrary i will consider it :thumbup:


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Re: Violet's V-Tek ??? [Re: blackout]
    #21964467 - 07/19/15 11:11 AM (8 years, 6 months ago)

Hmm, interesting about the gypsum.  I might should check out adding gypsum to agar for poms and stuff. Never thought about it, because I never saw much use in adding gypsum to my grain-only grows and abandoned it well before I started poms.  But maybe even that could take another look.

Apparently Bod doesn't care if he's shown wrong either, he ignores it and keeps going.
Or, if that was aimed at me, I wonder where it is you mean I "care" if "proven" wrong, or where I have been to begin with.
What I was getting at is that he will bug me and hound threads relevant to me about things he wouldn't bother you about. It's selective, to say the least. And using cutting verbiage like "stupidly" while trivializing what's actually very strong logic, while trying to sell the importance of skillsets regarding unnecessary steps.

I hope you're not saying te culture tek is "horrible" conditions? I'm not assumin you are, but anyways: IME & IMO the grain petris are far from horrible. They have plenty of GE considering no bulk subs, and small subs in small containment, and they retain constant humidity perfectly, with more than enough nutrition - a higher food density than a bulk/grain mix would have.


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Re: Violet's V-Tek ??? [Re: Violet]
    #21964490 - 07/19/15 11:17 AM (8 years, 6 months ago)

No I was saying an agar plate is horrible conditions. That's why I look for pins that can survive in them, usually means they do amazing in good conditions. As for the other comment, it was only in reference to myself.  I no longer compare people or methods. I try everything and I do what I do. I am no ones keeper. When I post its only in reference to what I know and what I do. At the same time I try to be open to all possibilities. That is the only way I can improve.

:sporedrop:


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21964582 - 07/19/15 11:38 AM (8 years, 6 months ago)

Oh, yeah agar pinning is a pretty poor condition. Though a large container helps. And poms help a lot! With those things combined agar can be used for a good full-fruiting method. When/if I get back in full swing, I have a version of the culture tech for poms. They work great for that.


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Re: Violet's V-Tek ??? [Re: Violet]
    #21964610 - 07/19/15 11:50 AM (8 years, 6 months ago)

While I have been unsuccessful at finding the proper scrubbies, my invitro straw method has really exceeded every expectation. Going to be running some more soon, just need to pony up more cash for the damn containers. I love them but I hate the price tag.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21964670 - 07/19/15 12:11 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Well when I make gw agar there is gypsum in it from the soak. I do find gw agar pins quite easy so maybe that is the reason. Tests will need to be done.



Right, I was since reading your posts where you talked of straining the gypsum out.

I suppose instead of trying to get a pin from MS you could transfer the same culture to various different plates with varying amounts of gypsum to better compare how it pins. I have an old jar (burma) which is pinning up the sides at the moment, I might test with some of these. The myc has crawled up the side of the jar and the pins are about 2 or 3 inches from the base where the grains are. I also have a mutant one growing from the grains with a big blog attached to its cap.


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Re: Violet's V-Tek ??? [Re: blackout]
    #21964680 - 07/19/15 12:14 PM (8 years, 6 months ago)

Agreed a stable culture would be needed to determine if it makes any difference at all. While I do strain the debris out, gypsum is soluble so I am sure that some remains regardless. I believe its solubility is greater at cool temps but that is easily worked around.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21964805 - 07/19/15 12:47 PM (8 years, 6 months ago)

I was also interested in creating pins in harsh environments. Especially in getting one which would grow in colder conditions, i.e. my own room temp which is lower than most.

This is Stephen L Peele talking about a "polar" strain/variety he developed

Quote:

If you mean the Psilocybe cubensis "Polar" strain...
That is a strain I developed that lives under colder conditions. It was easy....just kept dropping temp.
and collecting spores from the mushrooms that fruited. The last one to fruit did so at 39 F. It is still a Psilocybe cubensis species and nothing else. Did that answer your question? slp/fmrc


...It took a little over a year. The trick was I started with some that were fruiting in the winter out in a pasture. They were already fruiting at around 62 F. Don't forget I'm here in Florida. That may have knocked off a lot of time. slp/fmrc





Peele also talked of pouring hot/warm agar onto contaminated plates, the myc then grows through it and bacteria is meant to stay behind, then you transfer growth from the top.

This layering of agar had me interested. You could do all sorts, have weak and strong layers, layers of totally different recipies, antibacterial layers, peroxide layers etc if you thought they might help. People usually minimise the amount of spores they transfer to plates. I wondered what would happen if you did the opposite. Imagine printing directly onto the base of a jar, maybe even mulitple prints on top of each other so there are shitloads of spores. The layers of antibacterial agar might help with the now increased contaminant load.

Now you pour a very thin layer of warm agar on top and let it set, it should drop in temp very quickly and so not kill the spores. Stick it in a fridge to cool even more. Now that it is cool you could pour more warm agar and the prints should be insulated from the heat. Now place it in a quite cool place, and cold loving strains should grow up through the agar, which could be inches thick. It will hopefully leave any contaminants behind. I am not sure if this would work any better than plates but I suspect it has a harder time growing up through agar and so only the most aggressive out of 1000's of strains might make it to the top, and it might take weeks to do so.


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Re: Violet's V-Tek ??? [Re: blackout]
    #21964848 - 07/19/15 12:57 PM (8 years, 6 months ago)

Yes I have done warm pour and sandwich agar to escape contams myself. Interesting idea tho to use it to weed out the weak colonizers.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21965654 - 07/19/15 02:59 PM (8 years, 6 months ago)

Quote:

I am not sure if this would work any better than plates but I suspect it has a harder time growing up through agar and so only the most aggressive out of 1000's of strains might make it to the top, and it might take weeks to do so.




Very interesting.


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21965750 - 07/19/15 03:15 PM (8 years, 6 months ago)

Quote:

KauaiOrca said:
Quote:

Machiavelliavore said:
PE really likes a casing from what I've read, as does this tek.  Consider using one.




thanks for advice.  The way I'm approaching it is in 2 ways:

1) Using RGS and no casing for complete invitro growing to get the cleanest pins/clones and find a great culture.  This keeps the growing chamber (PP5 Quart jar) as contam free as possible.  Also,with the smaller PP5's, it can be used to generate really clean LI by just adding water to the colonized grain and sucking it back out.
2) Once some good cultures come from #1, then moving to WBR and casing to get the better fruits as the primary way of growing them out.

Just hoping to find one great culture that really likes this growing method.  I love how easy it can be, but I'm so early in the process I have no results to celebrate yet.




Much like I tried to do when I started out, you're trying to do everything at once so you're in the best position months in the future, which is probably why you're starting with PE and culturing instead of multispore cambo or whatever.

Since PE is supposedly a picky fruiter even on coir bulk, which has some of the properties of a casing, it seems to me that casing it on straight rgs would be good for production.  Since your invitro quarts use a whole cup, dedicating bunches at this early stage to just culturing without a casing and removing your best pins (?on a strain that produces few large dense fruits?) might gimp your harvest a bit.

Learning how to properly prepare and apply a casing is also a valuable thing to start learning right away.

For just culturing and printing, 1/8cup rgs in a 1 pint plastic screwtop uncased invitro might be preferable (as discussed in violets culturing tek.)

I mean, I'm assuming you like eating mushrooms :smile:

People that grow PE should definitely be regarded more than me in this matter.  Maybe it will fruit just fine off invitro grass seed, idk.

It's possible to outsmart yourself by trying to do everything at once.  I did this by doing some imediate isolation, didn't notice bits of non-sporulating trich growing in my grain master, and ruined a whole spawn run.  The isolates were all also garbage.  It caused a cascade of failures set into motion months ago that is only now resolving, just cause I didn't want to slum it with "multispore garbage." Not to imply that will happen to you, just a cautionary tale.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21965994 - 07/19/15 04:01 PM (8 years, 6 months ago)

I've had PE pin excellently from straight RGS.  It side-pinned badly when not invitro, however - just another mark chalked up to needing casing on grains for open fruiting, even RGS which doesn't need it invitro.


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Re: Violet's V-Tek ??? [Re: Violet]
    #21966185 - 07/19/15 04:52 PM (8 years, 6 months ago)

^  Well, if there's one person who knows about fruiting PE on grass seed invitro, it's her.

My experience of invitro fruiting uncased (even with GS) has been Sidepin City.  I have just used mason lids with a nail hole in them.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

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Re: Violet's V-Tek ??? [Re: Violet]
    #21966199 - 07/19/15 04:56 PM (8 years, 6 months ago)

Violet, isn't it some kind of social experiment of yours to see how many people use male or female pronouns to refer to you?  I feel like I recall hearing that from you at one point.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21966337 - 07/19/15 05:37 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
I didn't see you were doing it just to find clones. I'm not familiar with the method so I won't really say anything more other than there is no reason it can't work.




Pasty or Violet or Bodhi - I need some advice … so I've finally got some pins showing up on some invitro bags I'm doing … Tried a few strains, all done the same way LC > grains > Coir/Verm/Coffee/Gypsum back into bags  … now one bag has a lot of great pins growing some real big clusters right on top.  So …

In order to clone with the highest likelihood of success, should I take a small pin from the best cluster or should I let it grow out until they're bigger to determine which one to take a clone from?  And then, I put it on Agar, let it grow out, transfer until clean, make another clean LC and test it out?  I'm just looking for the best, highest probability most efficient way to get a good clone going.  The pin clusters in this one bag really look fantastic but I don't know if I should take a pin now or let them grow out then take some tissue from a bigger mushroom??????


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21966346 - 07/19/15 05:40 PM (8 years, 6 months ago)

Its really up to you and how you prefer to grow. I like to let the clusters grow just a little to give me an idea how even the fruits in them appear. . . some people prefer tiny pins. If the fruit/cluster is a bit more mature (not full blown dropping spores tho) then do a biopsy, for really small pins just drop em onto agar and isolate away from the contams.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21966381 - 07/19/15 05:46 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Its really up to you and how you prefer to grow. I like to let the clusters grow just a little to give me an idea how even the fruits in them appear. . . some people prefer tiny pins. If the fruit/cluster is a bit more mature (not full blown dropping spores tho) then do a biopsy, for really small pins just drop em onto agar and isolate away from the contams.




Since I'm new to agar, I think I'll try both as I don't really have much to lose and we'll see what happens.  It's fun to finally have some fruits to work with to find that magic clone culture everyone talks about.  This one seems to be fruiting fast as the WBS colonized fully in 8 days, then I broke it all  up and mixed it with Coir/Verm/coffee/Gypsum then put back in a bag it re-colonized and 18 days (total from breaking up WBS and mixing with substrate) later I'm getting pins.  Would you call that a pretty fast growing strain?


--------------------
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Edited by KauaiOrca (07/19/15 05:59 PM)


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21969600 - 07/20/15 07:47 AM (8 years, 6 months ago)

Quote:

bodhisatta said:
I prefer to clone my clones from something more similar to the conditions Im planning on growing in. An in vitro pinning on straight rgs or grain clone isn't the same as a clone from how you plan on doing your real grows. There's [gradient:#D4C7C7,#]no reason to be so stupidly concerned[/gradient] with how clean your clone is. If you cant clone without having to do it all in vitro then don't bother just get better at sterile techniques




I have no doubt you know 10x (maybe 100x) more than I do about every topic related to growing and I appreciate  your interest and time to reply.  I just wonder why you put it across in such an aggressive and caustic manner?  There's really no reason to fight about this stuff, is there?  Violet has a system that keeps all her culturing stuff very clean from contams which seems to me to be really good sterile technique that PREVENTS contams in the first place.  She seems to suggest that good performing clones in her system perform well no matter what growing system she uses later. 

I feel like I'm so inexperienced I have no credibility at all to doubt anyone's advice as all three of you have great ideas and appreciate the help.


--------------------
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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21969645 - 07/20/15 08:02 AM (8 years, 6 months ago)

If you never deal with contams then how can you know your sterile technique is any good?  There is a way to achieve good results even with dirty samples and never learning to do that cheats the grower in the end, which is probably why bod's so passionate about it, since I'm sure like many, it just irrationally pisses him off to see people do things inefficiently and worry about the wrong sets of variables.

I can't speak for him of course, but if I had to wager a guess.... that would be it.


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Re: Violet's V-Tek ??? [Re: Inocuole]
    #21969654 - 07/20/15 08:04 AM (8 years, 6 months ago)

That and attitude. I get along with 99% of people here just fine. Some people not so much because of how they act, also what they say, and the misleading nature of saying things that are to grab noob attention. That's why I'm done with V just on ignore for the rest of ever. She has some smart ideas but the box it comes in... Not worth it.


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Re: Violet's V-Tek ??? [Re: bodhisatta]
    #21969688 - 07/20/15 08:18 AM (8 years, 6 months ago)

Quote:

bodhisatta said:
That and attitude. I get along with 99% of people here just fine. Some people not so much because of how they act, also what they say, and the misleading nature of saying things that are to grab noob attention. That's why I'm done with V just on ignore for the rest of ever. She has some smart ideas but the box it comes in... Not worth it.




Like i said, I appreciate your interest and Ideas and I'm learning a lot.  Making huge progress last few months.  Trying a few things … getting into agar steps and am searching for the elusive great clone culture that seems to be a big part of the formula of consistently performing grows.  Since I have no interest in ever doing big mono tub grows, Violet's invitro culturing grows and Muda's bottle tek's are exactly what I was looking for.  Although I haven't generated fantastic results with them (too early in the process right now) I'm working on it and appreciate all the good suggestions and ideas.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21969704 - 07/20/15 08:24 AM (8 years, 6 months ago)

I can't imagine being at a point in my life where I could say I never want to grow a large monotub.  Craziness...

:mindblown:


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Re: Violet's V-Tek ??? [Re: Inocuole]
    #21969851 - 07/20/15 09:10 AM (8 years, 6 months ago)

Yet it's something that has been fairly commonly said, even by some of the bigger tub guys on here.

Orca, pay no mind to bodhisatta.  His complaints about his interactions with me are true only by his own making.  For well over a year he has purposefully, by his own declaration, picked fights with me, largely in the attempt to drive me away, again his words not my claim. I even get his "friends" message me sometimes with apologies on his behalf since he would never dare do so himself.
The issues he points to wouldn't even exist were it not for his poor choices. There was like a week some time ago where everything was nice after I confronted a group about it, and and it seemed he was going to be respectable and respectful.  But unfortunately yet predictably it wore off, and didn't take long before he went back to being hateful and cutting in that exact way, completely without provocation.


"If you never deal with contams how can you know your sterile technique is good?"
Gotta say, that seems like a pretty silly question to me.  Fixing something not broken?
If you never (seldom) deal with contams, it must be pretty clear that your sterile technique is beyond sufficient, and that you're doing everything right that's called for.
Sterile technique comes down to the technique used to grow sterile just as much as, if not moreso than, controlled motions in transfers.  Seldom dealing with contams means you've married the two ideally.
Jars and modified lids were used to turn certain facets of manual sterile tech into part of the process.  It's almost the very definition of Technology.  Methods like mine simply move the myco process even further down the path it's always been going - Intelligently getting the gear and process to do the job better and more easily.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Violet's V-Tek ??? [Re: Violet]
    #21969863 - 07/20/15 09:17 AM (8 years, 6 months ago)

Quote:

Violet said:
Yet it's something that has been fairly commonly said, even by some of the bigger tub guys on here.

Orca, pay no mind to bodhisatta.  His complaints about his interactions with me are true only by his own making.  For well over a year he has purposefully, by his own declaration, picked fights with me, largely in the attempt to drive me away, again his words not my claim. I even get his "friends" message me sometimes with apologies on his behalf since he would never dare do so himself.
The issues he points to wouldn't even exist were it not for his poor choices. There was like a week some time ago where everything was nice after I confronted a group about it, and and it seemed he was going to be respectable and respectful.  But unfortunately yet predictably it wore off, and didn't take long before he went back to being hateful and cutting in that exact way, completely without provocation.


"If you never deal with contams how can you know your sterile technique is good?"
Gotta say, that seems like a pretty silly question to me.  Fixing something not broken?
If you never (seldom) deal with contams, it must be pretty clear that your sterile technique is beyond sufficient, and that you're doing everything right that's called for.
Sterile technique comes down to the technique used to grow sterile just as much as, if not moreso than, controlled motions in transfers.  Seldom dealing with contams means you've married the two ideally.
Jars and modified lids were used to turn certain facets of manual sterile tech into part of the process.  It's almost the very definition of Technology.  Methods like mine simply move the myco process even further down the path it's always been going - Intelligently getting the gear and process to do the job better and more easily.




Violet - I've got an invitro MS bag I started a month ago pinning really nicely with some good clusters right in the middle (not sides) and my goal is to identify one of those killer prolific clone cultures … So, would you grab a small pin from one of the cultures then move it to agar then, RGS, then make a Grain LC for use with the WBR/Casing step or would you let the clusters grow out a bit, look for the healthiest one then take a bit of tissue from the base of one of the clusters and transfer that to the Agar?  I'm trying to decide which of the two (small pin vs. biopsy tissue from base of grown out cluster) is the highest probability way of getting a good performing clone culture.  Thanks!


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Edited by KauaiOrca (07/20/15 09:31 AM)


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InvisibleInocuole
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Re: Violet's V-Tek ??? [Re: Violet]
    #21970325 - 07/20/15 11:38 AM (8 years, 6 months ago)

Quote:

Violet said:
"If you never deal with contams how can you know your sterile technique is good?"
Gotta say, that seems like a pretty silly question to me.  Fixing something not broken?
If you never (seldom) deal with contams, it must be pretty clear that your sterile technique is beyond sufficient, and that you're doing everything right that's called for.




Perhaps if the rest of your technique serves as a crutch, but then when you go to try to clone a wild fruit or work with a print that might not be clean, it'll show.  I'm not interested in having an argument about it, I don't care how anyone else grows.  :shrug:


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21970352 - 07/20/15 11:49 AM (8 years, 6 months ago)

Personally I would pluck a small pin. They recover more quickly to colonize thick and healthy.  The biopsy can do the same mostly but I prefer pins.  If you do let them grow out, keep in mind that at a certain point the pin may continue growing, and open cap and sporulate.  Check photos in my culture tech to see what I mean.
Regardless of which you choose, remember that you need to spend at least two or three dishes transferring the fastest and healthiest growth to isolate before you bother putting to grains.  That's the more important part about cloning and isolating before testing, not necessarily how you took the clone.


Inocuole, there's nothing like a "crutch" about it, any more than any other technique.  You still have to have proper still-air-box/sterile airflow technique to avoid contams.  I don't see where you're getting reasoning for making such a harsh statement.


--------------------
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Re: Violet's V-Tek ??? [Re: Violet]
    #21970494 - 07/20/15 12:11 PM (8 years, 6 months ago)

I'm not even talking about your technique or anyone's in particular, but I guess if you took it that way, I could see how the statement is harsh.  However, I daresay it could potentially be easier for someone to get away with sloppy technique using your tek, maybe, if they wanted to be able to get away with it.  :shrug:

It's not always a bad thing.  I just like large grows for the most part and they inevitably require a different set of variables that some tend to shy away from.  I don't even know what all we're talking about in here anymore, there was just an element of what bod said that I resonated with.  Outside of that I don't have any beef and am not looking to start any.


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Re: Violet's V-Tek ??? [Re: Inocuole]
    #21970511 - 07/20/15 12:16 PM (8 years, 6 months ago)

"You should purposefully start with dirty samples or you won't appreciate agar."

If one wants to learn to clean samples, then yes.  If one just wants good clones, then maybe not.

Personally I've cloned whole fruits.  I've only done one invitro grow that did anything other than sidepin, it was cased, and it was pretty anemic anyway so I didn't want to clone from it.  It is also nice to see the whole fruit rather than the pin.  I've started ripping inner stipe tissue with tweezers, which I find much more effective than knife/scaple work.

That said, if I were looking to find a great isolate and testing a plethora of samples, violets pinplucking sounds like a great way to speed up the process.

If one was only gonna take one or two clones and role with them, whole fruits would probably be preferable.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21970590 - 07/20/15 12:46 PM (8 years, 6 months ago)

Thanks Violet & Mach -- That's exactly what I was looking for.  I'm going to do both.  Take a pin now and put it on agar and then when the biggest meatiest cluster grows out, snag some inner tissue with tweezers and put that on agar and see which one does best.

Appreciate the advice.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: Inocuole]
    #21971213 - 07/20/15 03:20 PM (8 years, 6 months ago)

Quote:

Inocuole said:
I'm not even talking about your technique or anyone's in particular, but I guess if you took it that way, I could see how the statement is harsh.  However, I daresay it could potentially be easier for someone to get away with sloppy technique using your tek, maybe, if they wanted to be able to get away with it.  :shrug:

It's not always a bad thing.  I just like large grows for the most part and they inevitably require a different set of variables that some tend to shy away from.  I don't even know what all we're talking about in here anymore, there was just an element of what bod said that I resonated with.  Outside of that I don't have any beef and am not looking to start any.





You can't get away with sloppy tek doing this. The only crutch I can think of is not using traditional pasteurization for the casing. It's microwaved.

Then again most people are using coco coir for mono subs. With the easy prep methods ( bucket tek, just sterilizing or using as is)...
... You're also skipping traditional pasteurization.

Everything else is the same. Petri work is the same. SAB work is the same. Transfers, gtg, etc etc....  all the same.
The containers and invitro are the only real difference.

PF cakes are the real crutch.


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Re: Violet's V-Tek ??? [Re: Violet] * 1
    #21971764 - 07/20/15 05:19 PM (8 years, 6 months ago)

Quote:

Violet said:

Orca, pay no mind to bodhisatta. 




nice write up!! agree looks like that bodhisatta has been on shroomery for 2 years and has like 15,000 posts he must seriously have no life.. which is why he follows you around on here


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Re: Violet's V-Tek ??? [Re: bakenast]
    #21976525 - 07/21/15 05:05 PM (8 years, 6 months ago)

Quote:

bakenast said:
Quote:

Violet said:

Orca, pay no mind to bodhisatta. 




nice write up!! agree looks like that bodhisatta has been on shroomery for 2 years and has like 15,000 posts he must seriously have no life.. which is why he follows you around on here




What's cool about it is how easy it is to have a lot of test cultures growing out simultaneously without having to fuss with a fruiting chamber or worry much at all about contams, dunking, rolling, etc. to see which clones will actually pin and grow.  A lot of creativity here on Shroomery and that makes it a lot easier for relative newbies to learn.

Bodhisatta gave me some good advice on cloning too … he definitely knows a lot about growing, no doubt about that.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21976645 - 07/21/15 05:31 PM (8 years, 6 months ago)

im buying my stuff in the morning at walmart to do this, and I hate the dunking and rolling part, or loosing a entire tub to a contam :smile:,and I was just baffled at that many posts in a couple years was all lol


Edited by bakenast (07/21/15 05:32 PM)


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Re: Violet's V-Tek ??? [Re: bakenast]
    #21976655 - 07/21/15 05:33 PM (8 years, 6 months ago)

People who give a lot of advice and answer a lot of questions tend to rack up a lot of posts. My own post count is pretty high too.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21976735 - 07/21/15 05:49 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
People who give a lot of advice and answer a lot of questions tend to rack up a lot of posts. My own post count is pretty high too.




P - Got my first pins on agar (started them today) based on your suggestions.  Gonna also take some tissue from a big cluster in a few days and pit it on agar too.  Hopefully, between those two, I'll get one really good performing clone culture.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21976862 - 07/21/15 06:24 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
People who give a lot of advice and answer a lot of questions tend to rack up a lot of posts. My own post count is pretty high too.



yes it is lol


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Re: Violet's V-Tek ??? [Re: bakenast]
    #21977048 - 07/21/15 07:10 PM (8 years, 6 months ago)

Quote:

bakenast said:
Quote:

Pastywhyte said:
People who give a lot of advice and answer a lot of questions tend to rack up a lot of posts. My own post count is pretty high too.



yes it is lol




Maybe you shouldn't insinuate that people who are pretty much exclusively here to help "have no life" just because you disagree with one of their opinions. :shrug:


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Re: Violet's V-Tek ??? [Re: Inocuole]
    #21977313 - 07/21/15 08:07 PM (8 years, 6 months ago)

Without the negative connotations, I kindof half-agree.  It's one thing when a person can show they're an incredibly grower, and pretty much a culture scientist and a bit of a tek artist like Pasty.  It's something else when people do seem to just post and post and post and have very little else to show, not even great grows to credify the massive anounts of advice they may give.
But this is coming from someone who believes in quality not quantity.  I'm happy to keep my post count low, kinda on purpose.

Orca, I hope you show your results with these teks, and KEEP showing your improvements!  There are starting to be a lot of people succeeding happily with the methods, but I wish more than just a few showed more of their stuff!


--------------------
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PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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Re: Violet's V-Tek ??? [Re: Violet]
    #21977349 - 07/21/15 08:14 PM (8 years, 6 months ago)

Well bod can be abrasive just like any of us but I know his post count isn't that way because of mindless spamming either.  Some of us like to talk about other stuff here too.  :shrug:


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Re: Violet's V-Tek ??? [Re: Violet]
    #21977641 - 07/21/15 09:10 PM (8 years, 6 months ago)

Quote:

Violet said:

Orca, I hope you show your results with these teks, and KEEP showing your improvements!  There are starting to be a lot of people succeeding happily with the methods, but I wish more than just a few showed more of their stuff!




V - I'm afraid my results will probably be nothing like the pictures I see here on this site.  I'll be absolutely stoked to find a strain that fruits nice and consistently invitro with your technique, so that a few stealthy bottles every once in awhile keeps me happy!  I can't even imagine what I would do with all the fruits I see growing in the monotub grow pics posted here.  Another day or two and my first 4 quart PP5's will get their tops cracked opened as they're nearly 100% colonized.  Got several multi spore small jars going on RGS for the culturing system so I'm 100% in!  Haven't had much luck in last 4 months with a couple of grows that just yielded tiny fruits, but I've got one bag going now with a SA strain that is pushing up a bunch of big ones!  Going to try the Grain LC method as well.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca] * 1
    #21981089 - 07/22/15 05:07 PM (8 years, 6 months ago)

I will admit that my V-tek results are not as good as violets but I am getting better. I think my biggest problem other than consistently overfilling the containers is grain size. While RGS is far too expensive for me to contemplate using regular, the rye and wheat are just not cutting it, too much dead space in the container. My next try is going to be with straight millet. I plan to give it a go here soon.

My current V tek attempt was with a clone not well suited either so that might be an issue. It is far better suited to bulk.



Second flush



I am really finding invitro straw hydrated with a water flour suspension to be good, gonna run a bunch more here soon too! It is hands down the cheapest way to grow. About a nickel per container to do.



Does well invitro as well, this was a third flush. . .



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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21981370 - 07/22/15 06:10 PM (8 years, 6 months ago)

those straw containers u've been posting really do perform well man.  i like that idea a lot.


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Re: Violet's V-Tek ??? [Re: blindingleaf]
    #21981385 - 07/22/15 06:13 PM (8 years, 6 months ago)

Yeah I need to still tweak a few more things but I am nearly satisfied. Gonna get some LC going soon so I can do some more.


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21982166 - 07/22/15 09:40 PM (8 years, 6 months ago)

Brown rice has quickly become my favorite grain because of how easily and quickly it strains and how easy it is to shake.  A few minutes of care to prevent bursting are well compensated for by the ease of everything else about it.  I grab it for 70c/lbs at bargainmart/dollarstore, cheap enough for my purposes at least.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21983532 - 07/23/15 08:03 AM (8 years, 6 months ago)

well i inoculated my seed this morning it appears that the seed seem clumpy  and alot of moisture, is this normal i followed this tek to a T and strain the seed very well after bringing to a steady boil for 25 minutes


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Re: Violet's V-Tek ??? [Re: bakenast]
    #21983566 - 07/23/15 08:16 AM (8 years, 6 months ago)

Quote:

bakenast said:
well i inoculated my seed this morning it appears that the seed seem clumpy  and alot of moisture, is this normal i followed this tek to a T and strain the seed very well after bringing to a steady boil for 25 minutes




Mine was a bit clumpy too when I inoculated it as I'm trying this for the first time too.  A lot of moisture in there dripping down the sides but no pooling water or anything like that.  But 5 days later, the RGS is fully colonized and looking great. Going to wait a few more days to crack open the lids a bit and see what happens.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21983604 - 07/23/15 08:31 AM (8 years, 6 months ago)

ok great i was kinda worried


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Re: Violet's V-Tek ??? [Re: bakenast] * 1
    #21984592 - 07/23/15 12:49 PM (8 years, 6 months ago)

IME, rgs takes a very long time to properly dry even if you don't overdo it on the prep.  To get it to the proper level of hydratation, I like to spread out out in a large tote or tote lid, and leave it outside in the sun, mixing it every half hour to hour.  It dries mostly through evaporation.  When you strain it, most of the dripping will have finished within 10-20 minutes.  Putting a towel in the tote may help.

I'm still never quite sure where the RGS is meant to be prepped.  When I use a large stock pot, grain squirts out when pinched between nails after a single boil, which I suspect means it's done or overdone.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21984633 - 07/23/15 12:59 PM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
IME, rgs takes a very long time to properly dry even if you don't overdo it on the prep.  To get it to the proper level of hydratation, I like to spread out out in a large tote or tote lid, and leave it outside in the sun, mixing it every half hour to hour.  It dries mostly through evaporation.  When you strain it, most of the dripping will have finished within 10-20 minutes.  Putting a towel in the tote may help.

I'm still never quite sure where the RGS is meant to be prepped.  When I use a large stock pot, grain squirts out when pinched between nails after a single boil, which I suspect means it's done or overdone.




I did my first RGS prep this month following Violet's instruction to the letter.  I have no idea if they were dry enough or not, but all of the jars (4) I inoculated with LC colonized very quickly and the 4 I did with a ms syringe are all colonizing nicely too, so, for me the grains seem to be working according to her directions.  I do live in a very dry climate and not sure that matters, but it is a factor.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21984678 - 07/23/15 01:05 PM (8 years, 6 months ago)

You can get away with a wide variety of moisture content in vtek containers and they will still work.

Glass RGS master quarts are another matter.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21984818 - 07/23/15 01:24 PM (8 years, 6 months ago)

Have you ever fruited PE strain invitro with this TEK and RGS and made it work?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21985005 - 07/23/15 02:04 PM (8 years, 6 months ago)

Nah, I only have two varieties and I live in one of the shitty states so people probably won't trade with me.

My only serious invitro grow was about 1/2-2/3 cup birdseed cased with 50/50+ in a quart mason.  I fruitied it with a tinfoil lid that i stabbed with a knife.  It has consistently seemed very prone to aborts that shit on its yield.  Not sure if that's from high CO2 levels or genetics (had a tray that loved to abort as well, no way it was because of CO2 in that case.)  Still, based on the difference between that, and the fruiting characteristics of my unattended grain petris (some rgs, some wbs,) I would seriously consider using a casing.

I'm very close to finishing my greenhouse, so it's pretty much gonna be regular v-tek from here on.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Violet's V-Tek ??? [Re: Machiavelliavore]
    #21985082 - 07/23/15 02:16 PM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
Nah, I only have two varieties and I live in one of the shitty states so people probably won't trade with me.

My only serious invitro grow was about 1/2-2/3 cup birdseed cased with 50/50+ in a quart mason.  I fruitied it with a tinfoil lid that i stabbed with a knife.  It has consistently seemed very prone to aborts that shit on its yield.  Not sure if that's from high CO2 levels or genetics (had a tray that loved to abort as well, no way it was because of CO2 in that case.)  Still, based on the difference between that, and the fruiting characteristics of my unattended grain petris (some rgs, some wbs,) I would seriously consider using a casing.

I'm very close to finishing my greenhouse, so it's pretty much gonna be regular v-tek from here on.




One of the things you hear over and over here is "a cube is a cube" but my experience is there is a big difference in terms of how different strains respond to invitro growing.  Some really hate it and others thrive which may have a lot to do with opinions that are formed in terms of whether you had a receptive strain.  I went through three recently that just didn't like it and wouldn't fruit right and finally found one that loves it.  Not doing anything different or better, just a strain that likes invitro and when that happens, it's pretty damned easy.  I know a lot of people get into the art and science of it all and are unbelievable creative, I'm just into an easy, stealthy grow that WORKS consistently, as I don't need or even want to harvest 10lbs of fruits … It seems a lot more manageable to have 4-5 different grows going in these plastic jars as you need almost no space to do it.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: Pastywhyte]
    #21989683 - 07/24/15 11:08 AM (8 years, 6 months ago)

Quote:

Pastywhyte said:
I usually just make my agar a little richer and it almost always pins from ms. 90% of the clones that I have kept long term came from pins off my agar.





Would you please elaborate? A little richer? Does this mean more LME in my case? You leave the spores long enough to pin? Sometimes the jargon isn't clear to me.

Thanks


--------------------
"He's not altogether dense, but he's not altogether there."


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Re: Violet's V-Tek ??? [Re: Ajahn Don]
    #21989792 - 07/24/15 11:20 AM (8 years, 6 months ago)

More nutritional content yes.


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #21989942 - 07/24/15 11:55 AM (8 years, 6 months ago)

Quote:

KauaiOrca said:
One of the things you hear over and over here is "a cube is a cube" but my experience is there is a big difference in terms of how different strains respond to invitro growing.



I mentioned this a few times in the "strain thread". Hippie3 tested out lots of different ones, if you search invitro PF or neglect tek you might find them.

Pf classic & gulf coast were 2 commonly recommended ones.

Growing in jars with more air space might give different results though.


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Re: Violet's V-Tek ??? [Re: Grey]
    #22328775 - 10/03/15 03:02 PM (8 years, 3 months ago)

Oh, THIS is where that SteveRogers has trolled me before. Now I remember.

Good read.  I might start collecting the good reviews from people who have actually done my teks right, like some that are in this thread.
Some stuff that I missed, too.
Quote:

Grey said:
I think some people are mixing up v-tek and violets invitro bottle tek. I've never tried v-tek, and tbh probably won't. However, the invitro tek she has works.



Yeah, they are kindof mixing them up.
But that's not really the point.  The irony is that they get treated so differently...
People don't get that they're almost THE SAME TEK!
One goes in a fruiting chamber and requires casing. The other IS ITS OWN fruiting chamber and doesn't require a casing unless using something other than grass seeds or BRF/verm (or any other bulk sub mix)
Past that, they're both the same bottom-watered grain cakes.  (Except using BRF/verm in which case it's bottom-watered PF cakes)
So, approval for one is approval for the other.
Of course, rather than dragging the unwarranted negative stigma from v-tek to the invitro tek, that should bring the understanding and acceptance of the invitro tek to the more scalable v-tek.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (10/03/15 03:11 PM)


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Re: Violet's V-Tek ??? [Re: Violet]
    #22328835 - 10/03/15 03:13 PM (8 years, 3 months ago)

cool :thumbup:


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Re: Violet's V-Tek ??? [Re: Mushroom_J]
    #22328923 - 10/03/15 03:27 PM (8 years, 3 months ago)

Quote:

Mushroom_J said:
I did brf cakes instead of rgs.
Multispore. 4 containers.
It's over all cheaper and same results as cakes in in a sgfc.
Don't need a fruiting chamber. I've averaged about a 1/2 oz off each container so far. . I think that's pretty good.

Some flushes were better than others.














:awethumb:


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Re: Violet's V-Tek ??? [Re: Violet]
    #22329279 - 10/03/15 04:37 PM (8 years, 3 months ago)

Quote:

Violet said:
Oh, THIS is where that SteveRogers has trolled me before. Now I remember.




Haha!  I think it's hilarious that you dug around looking for where he came from (I saw the recent posts too)

You do seem to have attracted your fair share of trolls from what I can see.

I do have a couple WBS v-tek containers going, amongst the other half-dozen or so things I'm messing around with on my mad scientist shelves.  :smile:


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Re: Violet's V-Tek ??? [Re: Sivarted] * 1
    #22329478 - 10/03/15 05:29 PM (8 years, 3 months ago)

Recent v-tek grow results with RGS ...





No SGFC, no Monotub, No dunking/rolling, no fanning, no misting ... hell yes it works!  Very easy way to grow successfully.  Around 250g wet per container.  15 cents worth of RGS per container.


Edited by KauaiOrca (10/03/15 05:34 PM)


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #22330361 - 10/03/15 09:25 PM (8 years, 3 months ago)

:cheers:


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Re: Violet's V-Tek ??? [Re: cronicr]
    #22330385 - 10/03/15 09:31 PM (8 years, 3 months ago)

Indeed.  Fine looking grow :thumbup:


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Re: Violet's V-Tek ??? [Re: KauaiOrca] * 1
    #22343802 - 10/07/15 01:28 AM (8 years, 3 months ago)

Kudos to Violet on the enormously thorough write-up on RGS! I've learned a lot and chuckled at the arguments and dramatic posts in the discussion. I've often had better luck with RGS than grains (although I do love whole brown rice) and have always been puzzled why RGS isn't more popular. I appreciate some people's struggle to get the moisture correct. Ten years ago I just measured dry grass seed and added water ... shaking like hell after PC-ing to distribute the overly wet soggy seed at the bottom with the upper dry seed. Recently I tried your seed prep, and it works great! It's super easy, and results in lush growth. Being an obsessive efficiency aficionado, I find the advantages of your tek interesting and valid. I'm using 2 cup Ziploc containers (they work great) and also polycarbonate Magenta GA-7 boxes I found in a warehouse at an old job (used for sterile plant tissue culture). The Ziploc containers have 2 inches of seed and my Magenta boxes have 3. Is there any downside to such a deep vessel? I'm tempted to spread the seed in a tray (An old habit. I'm used to fruiting thin layers of spawn), but don't want to mess with it and just give it a go. Thanks for any feedback anyone has to offer.         
                                             


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Re: Violet's V-Tek ??? [Re: RJ Tubs 202]
    #22344866 - 10/07/15 11:14 AM (8 years, 3 months ago)

Quote:

RJ Tubs 202 said:
Kudos to Violet on the enormously thorough write-up on RGS! I've learned a lot and chuckled at the arguments and dramatic posts in the discussion. I've often had better luck with RGS than grains (although I do love whole brown rice) and have always been puzzled why RGS isn't more popular. I appreciate some people's struggle to get the moisture correct. Ten years ago I just measured dry grass seed and added water ... shaking like hell after PC-ing to distribute the overly wet soggy seed at the bottom with the upper dry seed. Recently I tried your seed prep, and it works great! It's super easy, and results in lush growth. Being an obsessive efficiency aficionado, I find the advantages of your tek interesting and valid. I'm using 2 cup Ziploc containers (they work great) and also polycarbonate Magenta GA-7 boxes I found in a warehouse at an old job (used for sterile plant tissue culture). The Ziploc containers have 2 inches of seed and my Magenta boxes have 3. Is there any downside to such a deep vessel? I'm tempted to spread the seed in a tray (An old habit. I'm used to fruiting thin layers of spawn), but don't want to mess with it and just give it a go. Thanks for any feedback anyone has to offer.         
                                             




You will have no problem going with deeper (more than 2 inches) RGS in the containers.  If you want to fruit all invitro then the more RGS you put in, the less headroom, but it's easy to get around that with an SGFC or just put a plastic baggie on top of the container when the mushrooms get to the top.  I think they grow a lot better with more fresh air, anyway.  Casing them helps a lot too ... I just use vermiculite, which works great for me, but there are more sophisticated formulas out there.  I think RGS is the perfect grain for small easy grows.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Re: Violet's V-Tek ??? [Re: KauaiOrca]
    #22348127 - 10/07/15 11:29 PM (8 years, 3 months ago)

Quote:

KauaiOrca said:

You will have no problem going with deeper (more than 2 inches) RGS in the containers.  If you want to fruit all invitro then the more RGS you put in, the less headroom, but it's easy to get around that with an SGFC or just put a plastic baggie on top of the container when the mushrooms get to the top.  I think they grow a lot better with more fresh air, anyway.  Casing them helps a lot too ... I just use vermiculite, which works great for me, but there are more sophisticated formulas out there.  I think RGS is the perfect grain for small easy grows.





Thanks for your reply. I've not posted in ages but I read a lot. I'm going to go for it, and directly fruit in the containers. I've got some 50/50+ so will use it. I have one more question. Violet's "Invitro TEK, with BRF or Grass Seed" says ..."When fully colonized, crack the lids loose by turning counter-clockwise a couple inches." and then put under light. Does this mean skipping the one week consolidation period that we often wait for, following complete colonization? AKA the chewing period... How do you folks manage your timeline for in-vitro fruiting?


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Re: Violet's V-Tek ??? [Re: RJ Tubs 202] * 1
    #22348345 - 10/08/15 12:51 AM (8 years, 3 months ago)

I don't mention the consolidation period in the invitro ek specifically because it isn't necessary to. With grass seed, or a bulk sub style mix like BRF/verm is, consolidation isn't nearly as necessary or helpful as it is with grains like whole brown rice.
I think I mention it nearer the end, where it can be consolidated for more even first flushing and perhaps better potency.
But for the most part, they'll fruit when they're ready, and with GS or BRF/verm that wait isn't long enough to necessitate the container staying closed.  Those subs make it max simple. The invitro condition doesn't have any danger with cracking the lid a little early if they wouldn't fruit right away which they often don't anyway.  But the increased air can help them pin a bit sooner since it speeds metabolism.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (10/08/15 08:03 AM)


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Re: Violet's V-Tek ??? [Re: Violet]
    #22351963 - 10/08/15 08:53 PM (8 years, 3 months ago)

Great. Thanks for the information Violet.


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