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OfflinePurePleasure
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Registered: 08/29/14
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Re: contam resistance [Re: PurePleasure]
    #21927255 - 07/11/15 03:10 AM (8 years, 6 months ago)

Then again it could just spawn mutants :shrug:
I've toyed around with alot of these kinds of things and there isn't really no telling without putting it to the test but with my experience, my wild gulf coast print in first got was full of Mold spores by the time I printed it. When I put spores to agar I let it rode. Bacteria Trich, stachyubacus  (or w/e) and asp Niger were all on the plate. I let It go until a wall of mycelium formed, some mycelium died, some mycelium held them all off and fruited. I cloned the fruit and it seems like that strain is highly  bacterial resistant along with other contams. When endospores took over my other strain jars with the same media in it, the gulf Coast usually either walled off and colonies that area within or covered the bacteria and I've even seen it cover trich like It was nothing.
Just a theory though :shrug:
I actually save a plate my gulf Coast had trich next to and grew over it and stopped it like it was nothing.


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If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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OfflinePurePleasure
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Re: contam resistance [Re: Oeric McKenna]
    #21927256 - 07/11/15 03:11 AM (8 years, 6 months ago)

Quote:

Oeric McKenna said:
Just because theres a pin doesnt mean the mold didnt kick the mycs ass.. which it did. 
If it ate the mold or something I'd say it might be worth that idea.
Like I said, this is common.
It ran with the mold and lost. Kicked out a pin before it died.



It did not loose against the mold because it pinned instead of being eaten as fuel.


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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InvisibleOeric McKenna
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Re: contam resistance [Re: PurePleasure]
    #21927271 - 07/11/15 03:16 AM (8 years, 6 months ago)

No dude, it pinned before being eaten as fuel...
Thats very common. The point of the mushroom is reproduction and the mycelium will fruit at any stage of development if its about to die. Its a self preservation thing.
Its why jars that have contams fruit before they're done!
The mycelium is dying and throwing its last shot at species survival through its offspring


--------------------


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love is everything
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InvisibleOeric McKenna
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Re: contam resistance [Re: Oeric McKenna]
    #21927282 - 07/11/15 03:20 AM (8 years, 6 months ago)

Dont get me wrong guys. Clone whatever you want but I wouldnt even open that moldy ass thing in the house.
As far as species are concerned, cubensis isnt known to win the mold war.
If mold was that big of a problem in a growers bulk, another avenue like playing with ph would be more effective than trying to clone some mythical super strain.


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OfflinePurePleasure
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Re: contam resistance [Re: Oeric McKenna]
    #21927306 - 07/11/15 03:34 AM (8 years, 6 months ago)

Quote:

Oeric McKenna said:
No dude, it pinned before being eaten as fuel...
Thats very common. The point of the mushroom is reproduction and the mycelium will fruit at any stage of development if its about to die. Its a self preservation thing.
Its why jars that have contams fruit before they're done!
The mycelium is dying and throwing its last shot at species survival through its offspring



But the pin didn't get eaten as fuel it looks self stabilized and clonable. And how do you know it pinned before it molded out? It tends to take around a month to see fruits on agar. Other molds are much faster.
All im.saying is that pin deserves a chance. It put up one hell of a fight and earned it's mass it's taking up in this universe. Expand it azur. I have faith in you and your poo


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If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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Offlinehamloaf
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Re: contam resistance [Re: azur] * 1
    #21927454 - 07/11/15 05:00 AM (8 years, 6 months ago)

From what is understood, pins forming out of moldy substrates is merely a defense mechanism in an attempt by the culture to propagate it's self before imminent death.  NOT a symbol/symptom of a culture, and/or fruit body's resistance to mold.  Furthermore, clones can still have several (up to 20 or so) different strains of mycelium, so you still aren't exactly sure what's going to transcribe, genetically, when clones are taken.  It's also agreed with the poster above who stated that they wouldn't open that plate in the lab.  Not to mention the amount of potential transfers required before a clean culture culminated from pin generated in a plate full of mold(s) is a deterrent, as well.  Expand off of a pin that generated from a clean culture.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisiblecArcace-x
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Re: contam resistance [Re: hamloaf]
    #21927717 - 07/11/15 07:32 AM (8 years, 6 months ago)

Quote:

hamloaf said:
From what is understood, pins forming out of moldy substrates is merely a defense mechanism in an attempt by the culture to propagate it's self before imminent death.  NOT a symbol/symptom of a culture, and/or fruit body's resistance to mold.




that was my first though
maybe it fruit to try to ensure the survival of the specie since its all contamd! :tongue:

is the plate was in the fridge azur?


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Invisibleeatyualive
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Re: contam resistance [Re: PurePleasure]
    #21927891 - 07/11/15 08:34 AM (8 years, 6 months ago)

Quote:

PurePleasure said:
Quote:

azur said:
Quote:

Grey said:
GT has been really fun to grow for me. It always puts out.



It really does. And 2.5 grams of em is the perfect quintessential trip



I was a die hard gt fan until i had ksss.
Ksss is amazing.
I like it better than PE



QFT.


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EAT GETS SHIT DONE


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Invisibleazur
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Re: contam resistance [Re: eatyualive]
    #21927906 - 07/11/15 08:39 AM (8 years, 6 months ago)

I don't like pe.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblecArcace-x
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Re: contam resistance [Re: azur]
    #21928122 - 07/11/15 09:35 AM (8 years, 6 months ago)

i never tried PE!!!!!
:loldongs:


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InvisibleOeric McKenna
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Re: contam resistance [Re: cArcace-x]
    #21929116 - 07/11/15 02:33 PM (8 years, 6 months ago)

Its the bomb... if it colonized a long time it'll really kick yer ass into space.


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
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InvisiblePastywhyteMDiscord
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Re: contam resistance [Re: Oeric McKenna]
    #21929183 - 07/11/15 02:51 PM (8 years, 6 months ago)

Only thing that blows my hair back more than PE is. . . APE :hehehe:


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Invisibleeatyualive
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Re: contam resistance [Re: Pastywhyte]
    #21929802 - 07/11/15 05:14 PM (8 years, 6 months ago)

the best. pan cambodg blows my mind.

1 gram has me floating on clouds. no more walking after that. think with your mind and you will float.
:evil:


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Invisibleazur
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Re: contam resistance [Re: eatyualive]
    #22171828 - 08/31/15 10:53 PM (8 years, 4 months ago)

Hey fellas.
Finally got that plate cleaned up. It took....3 or 4 transfers. I don't remember. It was fun, though not really a top priority, so mold was trying to win each round as i let the plates sit for a while in between transfers.
Anyways, i didn't take a pic because, well, i forgot and also, our phones are filthy. I'll take a pic tomorrow as I'm done working tonight.
But i just knocked up one half gallon jar of it. Excited as i haven't grown gt in a long time and it's my personal favorite to consume.
Stay tuned.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleNumeroEno
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Re: contam resistance [Re: azur]
    #22173792 - 09/01/15 12:18 PM (8 years, 4 months ago)

I have some of your PE on a plate, and it's knotting like crazy off of a spot of trich. Once a pin develops from one of the trich knots I'll transfer it and clean it up.




--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Invisibleazur
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Re: contam resistance [Re: NumeroEno]
    #22174203 - 09/01/15 02:20 PM (8 years, 4 months ago)

:vibin:


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleGrey
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Re: contam resistance [Re: azur]
    #22174217 - 09/01/15 02:22 PM (8 years, 4 months ago)

I never did clone that GT pin. I ended up cloning a PESA pin on a plate "Mom" sent me.


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OfflineKizzle
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Re: contam resistance [Re: NumeroEno]
    #22182134 - 09/03/15 09:01 AM (8 years, 4 months ago)

Quote:

NumeroEno said:
I have some of your PE on a plate, and it's knotting like crazy off of a spot of trich. Once a pin develops from one of the trich knots I'll transfer it and clean it up.






I'm pretty sure that's not Trich though. If I had to guess I'd say Alternaria which is really only a common contaminant on agar, not something people are losing substrates to. Different molds produce different metabolites so being resistant one species doesn't say much as to if it would be resistant to another.


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InvisibleNumeroEno
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Re: contam resistance [Re: Kizzle]
    #22182169 - 09/03/15 09:20 AM (8 years, 4 months ago)

I just figured it was trich because of the high spore load at my place. I know you know your molds though :thumbup:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisibleazur
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Re: contam resistance [Re: azur]
    #22289482 - 09/25/15 05:43 PM (8 years, 4 months ago)

Quote:

azur said:
Just found this plate.
can't recall the date
very old
And covered in mold
It'd be a sin
Not to clone this pin

Do you  think this guy is stronger than your average mushroom considering how much mold is on the plate?  Imma find out. Tis going on agar tomorrow.







Hey guys. I have a tub of this gt I'm putting into fruiting tomorrow. Put some more on grain just a few minutes ago. Here's what it looks like now


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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