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InvisibleNumeroEno
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Registered: 07/24/14
Posts: 9,652
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Re: contam resistance [Re: azur]
    #21922652 - 07/09/15 11:53 PM (8 years, 6 months ago)

I just poured a sleeve of antibiotic plates to start some wild prints, and didn't use them all. The remaining 5 are going with me to Kentucky where I hope to take some wild clones. My friend tells me a ton of great edibles grow out there and we're going hunting the day after I get there.

I've been waiting for more stuff to start pinning on agar. Now I've had cambo, GT, and ape do it.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisibleazur
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Re: contam resistance [Re: NumeroEno]
    #21922658 - 07/09/15 11:54 PM (8 years, 6 months ago)

Nice. I always had pe and ape pinning on agar. I'm excited about this little bugger though


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleNumeroEno
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Registered: 07/24/14
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Re: contam resistance [Re: azur]
    #21922662 - 07/09/15 11:56 PM (8 years, 6 months ago)

This is actually the second pin that plate put out, but I didn't have plates ready for the first one and it shriveled up. I'm stoked I got a second chance.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisibleazur
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Re: contam resistance [Re: NumeroEno]
    #21922679 - 07/09/15 11:59 PM (8 years, 6 months ago)

Hells yea.
I'm crashing mang. Must gain sleep. Turning off pc after a 2 1/2 cycle. Been up since 6am and had children most if the day.
Agar tomorrow and I'll post a pic. Have a good night


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleNumeroEno
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Registered: 07/24/14
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Re: contam resistance [Re: azur]
    #21922707 - 07/10/15 12:09 AM (8 years, 6 months ago)

Cheers man! I've got to get some sleep too. Have to be up at 6 and work a full shift, then going to see widespread panic with a head full of these chocolates I just finished.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: contam resistance [Re: SteveRogers]
    #21922822 - 07/10/15 12:46 AM (8 years, 6 months ago)

Quote:

SteveRogers said:
Clone it for Bill O'reilly




--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleuncle_rico
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Re: contam resistance [Re: azur]
    #21922903 - 07/10/15 01:41 AM (8 years, 6 months ago)

Guess I should follow along as well.

It seems like every plate I haven't refrigerated, is pinning.



bunch of knots !  looks like it will be a nice "pinset".

I'll put something from this plate on fresh agar and keep you guys company.


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OfflineKizzle
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Re: contam resistance [Re: azur]
    #21922936 - 07/10/15 02:01 AM (8 years, 6 months ago)

Quote:

azur said:
Just found this plate.
can't recall the date
very old
And covered in mold
It'd be a sin
Not to clone this pin

Do you  think this guy is stronger than your average mushroom considering how much mold is on the plate?  Imma find out. Tis going on agar tomorrow.





If there's nothing to compare it to it could just as easily be weaker than average mold. :shrug:

I don't suppose those are reusable petris. If they are you can resterilize to kill the mold and mycelium and reinoculate it with a few different strains and see which does better. Even though the mold itself would be dead the metabolites it produced while competing with the mushroom mycelium would still present in the agar.


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InvisibleGrey
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Re: contam resistance [Re: Kizzle]
    #21924029 - 07/10/15 11:02 AM (8 years, 6 months ago)

I've got this GT plate.no mold though. I've just been letting it do it's thing.



--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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InvisibleNumeroEno
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Re: contam resistance [Re: Grey]
    #21924497 - 07/10/15 01:25 PM (8 years, 6 months ago)

You should put some of that mushroom on a new plate, and take a print from it as well. Join our little GT pin clone grow along lol.

It's all the same GT too, since I got mine from azur and you got that plate from me :cool:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


Extras: Filter Print Post Top
Invisibleazur
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Posts: 28,103
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Re: contam resistance [Re: NumeroEno]
    #21924504 - 07/10/15 01:28 PM (8 years, 6 months ago)

:vibin:
I won't be able to cook up any agar for a few hours. But definitely doing it today.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleGrey
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Re: contam resistance [Re: azur]
    #21924521 - 07/10/15 01:32 PM (8 years, 6 months ago)

I'll clone one. I gotta pour plates first. I'll try and do that tonight.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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InvisibleJuiceh
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Re: contam resistance [Re: azur]
    #21925170 - 07/10/15 04:05 PM (8 years, 6 months ago)

Quote:

azur said:
Do you  think this guy is stronger than your average mushroom considering how much mold is on the plate?



I asked a similar question 2 years ago and TL said it would not be any more contam resistant than normal.


--------------------
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Invisibleazur
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Re: contam resistance [Re: Juiceh]
    #21925240 - 07/10/15 04:20 PM (8 years, 6 months ago)

Did he say why?


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineKizzle
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Re: contam resistance [Re: Juiceh]
    #21925242 - 07/10/15 04:21 PM (8 years, 6 months ago)

Quote:

I asked a similar question 2 years ago and TL said it would not be any more contam resistant than normal.



And I bet they based that assumption on diddly squat.

Check this out. You can see resistance to the mold develop simply from exposure to the enzymes. It doesn't prove anything but it shows it's at least a credible possibility.
http://www.mycologia.org/content/95/2/191.full


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InvisibleJuiceh
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Re: contam resistance [Re: azur]
    #21926498 - 07/10/15 10:38 PM (8 years, 6 months ago)



--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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OfflinePurePleasure
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Re: contam resistance [Re: azur]
    #21927198 - 07/11/15 02:45 AM (8 years, 6 months ago)

Quote:

azur said:
Quote:

Grey said:
GT has been really fun to grow for me. It always puts out.



It really does. And 2.5 grams of em is the perfect quintessential trip



I was a die hard gt fan until i had ksss.
Ksss is amazing.
I like it better than PE


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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InvisibleOeric McKenna
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Registered: 06/15/12
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Re: contam resistance [Re: PurePleasure]
    #21927204 - 07/11/15 02:48 AM (8 years, 6 months ago)

Idk man... fruiting on agar is not too uncommon. The presence of mold is also coinciding with the end of a life cycle which is a last minute push to fruit.  Basically, I dont think theres probably much special about the pin other than that it was the mycs last ditch dying effort. Same as a contammed cake or any other similar scenario


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spread love
love is everything
2013 finds
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OfflinePurePleasure
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Re: contam resistance [Re: Oeric McKenna]
    #21927242 - 07/11/15 03:02 AM (8 years, 6 months ago)

Quote:

Oeric McKenna said:
Idk man... fruiting on agar is not too uncommon. The presence of mold is also coinciding with the end of a life cycle which is a last minute push to fruit.  Basically, I dont think theres probably much special about the pin other than that it was the mycs last ditch dying effort. Same as a contammed cake or any other similar scenario



That pin is going to have more buffed up genetics than any other section of mycelium in that plate.
It should be a fast colonizer And thick fruiter  I think. If not a bunch of sparce  thing fruit canopies, then rather fewer bulky as fuck and big mushrooms.
Only one way to tell.
As for contam resistance, that section of mycelium was able to produce the proper metabolites to defend against bacteria or whatever, so yeah I believe it will be less likely to be taken over by bacteria that is present in all off our grows just because it's in its genetics to know to fight it and how.


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)




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InvisibleOeric McKenna
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Re: contam resistance [Re: PurePleasure]
    #21927253 - 07/11/15 03:07 AM (8 years, 6 months ago)

Just because theres a pin doesnt mean the mold didnt kick the mycs ass.. which it did. 
If it ate the mold or something I'd say it might be worth that idea.
Like I said, this is common.
It ran with the mold and lost. Kicked out a pin before it died.


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


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Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom


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