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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 1 day, 3 hours
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
#23945942 - 12/21/16 12:08 PM (7 years, 1 month ago) |
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It only feels right to thank you bodhisatta, thanks to this tek and a few Youtube videos I have now my first growth on agar.
Reishi 
Ps. cyanescens 
AA+ (monokaryotic mycelium?) 
Ps. galindoi
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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LowcountryHunter
Stranger


Registered: 09/03/09
Posts: 149
Last seen: 2 years, 11 months
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Re: pour agar TEK [Re: Tiamo]
#23946049 - 12/21/16 12:49 PM (7 years, 1 month ago) |
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So how you cut the saran wrap roll?
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 1 day, 3 hours
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It's a 30 cm wide saran wrap roll. I cut it lengthwise in ~7cm strips. So then I have a strip 7cm wide*30cm long and it almost perfectly fits around a 90mm petri with some stretching.
Like this:
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: pour agar TEK [Re: Tiamo]
#23947209 - 12/21/16 07:42 PM (7 years, 1 month ago) |
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 Nice work tiamo
I use a chefs knife. Kept chef sharp. Goes right thru
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LowcountryHunter
Stranger


Registered: 09/03/09
Posts: 149
Last seen: 2 years, 11 months
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Wait, I thought you were talking about cutting thin sections of the whole roll, core and all...?
Also, any issue with pouring a bunch of plates inside the SAB then taking the first several from the stack (Coolest) and dropping a drip directly from a flame sterilized spore syringe in the center during the same SAB session. I would imagine the agar would be ready to use almost immediately. It seems like the agar would be cool enough to not kill spores. It just seems reasonable to take advantage of the time to kill two birds with one stone.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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That's what I do. Tiamo does it the hard way lol
And once the agar solidifies you can use it
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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bump
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
enlightenment said: Without description:

Spore solution:

thanks man
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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 MS GT plate pinned the shroom is too mature to clone though, but cool picture MS TC plate pinned too(not pictured) took the pins to agar
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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big update
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wooji19
Stranger


Registered: 08/04/14
Posts: 35
Last seen: 1 month, 4 days
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Starting agar work today, thank you for all of this.
-------------------- Misanthrope.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: pour agar TEK [Re: wooji19]
#24043547 - 01/27/17 11:41 AM (7 years, 2 days ago) |
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Nice update!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: pour agar TEK [Re: wooji19]
#24044627 - 01/27/17 05:06 PM (7 years, 1 day ago) |
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Quote:
wooji19 said: Starting agar work today, thank you for all of this.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Happy national pour plates day
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foodguy
MycoMealMaker
Registered: 01/05/17
Posts: 106
Last seen: 2 years, 11 months
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Just got one of these media bottles. Can't wait to compare to my erlenmyer.
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Servant
Stranger

Registered: 11/10/16
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Last seen: 4 years, 1 month
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Re: Bod's Comprehensive Agar TEK [Re: bodhisatta]
#24134528 - 03/03/17 08:20 PM (6 years, 10 months ago) |
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Suppose one were to have condensation, is the agar petri dish still usable?
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Bod's Comprehensive Agar TEK [Re: Servant]
#24135168 - 03/04/17 04:47 AM (6 years, 10 months ago) |
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Of course
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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CapnZ
Dimensional explorer



Registered: 12/22/16
Posts: 1,420
Loc: In the Mystic
Last seen: 3 months, 13 days
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Re: pour agar TEK [Re: foodguy]
#24135392 - 03/04/17 07:26 AM (6 years, 10 months ago) |
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Quote:
foodguy said: Just got one of these media bottles. Can't wait to compare to my erlenmyer.
I'm using a 1 L Erlenmyer. May have to check out the media bottles. They are PC friendly right?
-------------------- Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: pour agar TEK [Re: CapnZ]
#24135417 - 03/04/17 07:36 AM (6 years, 10 months ago) |
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Of course
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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CapnZ
Dimensional explorer



Registered: 12/22/16
Posts: 1,420
Loc: In the Mystic
Last seen: 3 months, 13 days
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Re: pour agar TEK [Re: Kenetic]
#24135466 - 03/04/17 07:58 AM (6 years, 10 months ago) |
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Quote:
bodhisatta said:
Quote:
enlightenment said: Without description:

Spore solution:

You can also use a sterile swab for spore solution correct? Any opinions on which is better, loop or swab?
-------------------- Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...
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