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Invisibleblindingleaf
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23225137 - 05/14/16 08:31 AM (7 years, 8 months ago)

pour agar FTW


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleJuiceh
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: blindingleaf]
    #23225171 - 05/14/16 08:40 AM (7 years, 8 months ago)

Last week I ran 2 media bottles of MEA and GWA. I totally forgot to loosen the caps after screwing them on tight and swirling the powdered ingredients into the hot water. The bottles and caps had no issues surviving a 25 min 20psi run this way. If you do this though, loosen the caps slowly in front of your hood. The agar will be bubbling under vacuum a little and they will suck in air to equalize the pressure and boil up a bit, so loosen and tighten until the agar settles and repeat until it doesn't bubble up anymore. Oddly the MEA took about 5-6 loosen and tightening of the cap to equalize while the GWA was equalized in 2 tries. Dunno if that means anything at all.


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How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: Juiceh]
    #23225296 - 05/14/16 09:21 AM (7 years, 8 months ago)

Some agar media is more foamy than others. Lin's media foams over like fucking mad. Everything cubes grow on is pretty tame.


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OfflineJahnobi
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23341084 - 06/13/16 08:51 PM (7 years, 7 months ago)

Really informative write up. Excited at getting into agar workk


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: Jahnobi]
    #23343704 - 06/14/16 02:48 PM (7 years, 7 months ago)

Best of luck, keep us posted


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Invisibleblackdust
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Re: pour agar TEK [Re: bodhisatta]
    #23344322 - 06/14/16 05:37 PM (7 years, 7 months ago)

good material

:awethumb:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: blackdust]
    #23347114 - 06/15/16 12:10 PM (7 years, 7 months ago)

:rockon:


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InvisibleSpellbound
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23482966 - 07/27/16 04:56 PM (7 years, 5 months ago)

Lolol

"Some of the kids are awesome, some are stupid" .... Brilliant hahahaha :thumbup:

Brill writeup too :smile:


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Mescaline Tea - the one singular occasion of my entire life I cooked for 9 hours :lmafo:

The trick is to be yourself, in a world trying to make you like everyone else :heart:



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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: Spellbound]
    #23484827 - 07/28/16 08:07 AM (7 years, 5 months ago)

You want to keep the ones with highest earning potential. Don't want to end up in a nursing home


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Invisibledankington
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23699676 - 10/02/16 09:56 AM (7 years, 3 months ago)

I've made the switch. Thanks for helping Bodhi. I simply didn't have the confidence before, but anyone can do this.

   

I'll get a proper media bottle later. :wink:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: dankington]
    #23699682 - 10/02/16 09:59 AM (7 years, 3 months ago)

:dancer:
It's easy


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OfflineSarah Tonin
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23699793 - 10/02/16 10:38 AM (7 years, 3 months ago)

When you sterilize (sanitize?) pyrex plates in foil in the oven, how long and at what temp?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: Sarah Tonin]
    #23699830 - 10/02/16 10:49 AM (7 years, 3 months ago)



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OfflineSarah Tonin
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23700010 - 10/02/16 12:00 PM (7 years, 3 months ago)

Thanks Bodhi, great link.  I have a stack of pyrex petri's wrapped in foil in my AA, now cool enough to access.  I hear you saying they will be wet - I didn't think about wrapping towel inside there.  I will dry them out in the oven with this method before opening the foil.

:jamming:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: Sarah Tonin]
    #23700076 - 10/02/16 12:32 PM (7 years, 3 months ago)

Yea I prefer baking glass just so it is dry


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OfflineSarah Tonin
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23700606 - 10/02/16 03:15 PM (7 years, 3 months ago)

That, plus free's up PC space for more bottles of agar :awesomenod:  Definitely implementing this tek, especially in winter when nice to have oven on.  The "roll" of plates fit perfectly in a bread pan :lol:.  Cooled them a bit and slid into a Ziploc.  Pouring them soon  :rockon: 

:thanx: :cheers:


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Offlinewtfcrazymofo
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Re: pour agar TEK for preparing petri dishes, using them, and storage on the EZ [Re: bodhisatta]
    #23701100 - 10/02/16 05:53 PM (7 years, 3 months ago)

I dig the bit about putting a drip on the loop.  I used to make minor floods.  Nice links.  :thumbup:


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineCann_Chi_Man
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Re: pour agar TEK [Re: wtfcrazymofo]
    #23701753 - 10/02/16 09:37 PM (7 years, 3 months ago)

Thank you for the ideas and info


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OfflineCann_Chi_Man
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Re: pour agar TEK [Re: Cann_Chi_Man]
    #23703352 - 10/03/16 11:52 AM (7 years, 3 months ago)

Thanks for sharing!


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OfflineGreg
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Re: pour agar TEK [Re: Cann_Chi_Man]
    #23703397 - 10/03/16 12:05 PM (7 years, 3 months ago)

Stopping by to say thanks for this awesome resource!
It really helped me get started with agar.

Now look at the mess I've gotten myself into...


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