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InvisibleJuiceh
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Re: pour agar TEK [Re: blindingleaf]
    #22306934 - 09/29/15 07:38 AM (8 years, 3 months ago)

I think those are the same bottles that aloha uses. Right?


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Invisibleblindingleaf
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Re: pour agar TEK [Re: Juiceh]
    #22307130 - 09/29/15 08:53 AM (8 years, 3 months ago)

yup.  they get the bottles before production, so no paper label to tear off.


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Re: pour agar TEK [Re: bodhisatta]
    #22315457 - 09/30/15 05:57 PM (8 years, 3 months ago)

Could I reheat agar in a toaster oven or oven? I know this sounds kind of stupid but I don't have a microwave


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: MysticMoteToter]
    #22315589 - 09/30/15 06:24 PM (8 years, 3 months ago)

No


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InvisibleTrek222
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Re: pour agar TEK [Re: bodhisatta]
    #22358455 - 10/10/15 10:24 AM (8 years, 3 months ago)

Hi Bodhi,



I finally received a new pressure gauge. I bought the sterilizer gauge that Juiceh recommended.  I sterilized my agar for 30 minutes at 15 PSI with the lid to the pyrex media bottle slightly loose. 



I sterilized Q-tips for 30 mins at 15 PSI for the initial transfer of spores to agar because my print was on paper.  This is how my plates look after the second transfer.



I will do additional transfers soon.

The bottom right hand plate was my first transfer from spore print. All of my first plates had tomentose growth.  By the second transfer rhyzomorphic mycelium was visible. 

Thanks for posting your Pour Agar Tek and sharing the pyrex media vessel that you use.  I really like the bottle.


Edited by Trek222 (10/10/15 10:29 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: Trek222]
    #22358942 - 10/10/15 12:19 PM (8 years, 3 months ago)

:rockon:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: bodhisatta]
    #22594081 - 11/30/15 10:32 AM (8 years, 1 month ago)

bump, added a little to the OP


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OfflinetaGyo
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Re: pour agar TEK [Re: bodhisatta]
    #22594367 - 11/30/15 11:58 AM (8 years, 1 month ago)

On the EZ?

:lmafo:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: taGyo]
    #22594380 - 11/30/15 12:02 PM (8 years, 1 month ago)

sure let's do it

and put the pussy on the chain wax too


Edited by Trusted cuItivator (11/30/15 12:03 PM)


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OfflinetaGyo
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Re: pour agar TEK [Re: bodhisatta]
    #22594400 - 11/30/15 12:06 PM (8 years, 1 month ago)

I haven't poured plates in forever, been playing with my pasty plates and working out how to avoid buying my next shipment of petris all together.

Definitely doable but I prefer the ease in wedges versus cutting a plate. I noticed a slight increase in bacteria (As I will unveil later today or early tomorrow) while dropping pasty plates into LIs. I just did one from a wedge of ESS and Oakridge and both are colonized beautifully. My TMF has bacteria which was a pasty plate. Ran that experiment a few more times.

In short, I'll be pouring soon :thumbup:

How'd you find those exact temperatures or did you just ball park based on previous research?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: taGyo]
    #22594448 - 11/30/15 12:18 PM (8 years, 1 month ago)

pouring agar at 47 is in a lot of lab SOPs for agar work. for that very reason that it's just about as cool as you can go before it turns into gel which means you'll get the least amount of condensation.

pour a stack of dishes with an oven glove on and the agar at 140F or something and it will look like rain inside of your petris


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OfflinetaGyo
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Re: pour agar TEK [Re: bodhisatta]
    #22594456 - 11/30/15 12:19 PM (8 years, 1 month ago)

Been there done that :lmafo:

Awesome, great info. So cool to the hand or slightly warm?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: taGyo]
    #22594460 - 11/30/15 12:21 PM (8 years, 1 month ago)

it's about 117F so pretty warm to the hand, that's the other benefit is you don't have to wear a glove to pour because it wont burn


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OfflinetaGyo
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Re: pour agar TEK [Re: bodhisatta]
    #22594484 - 11/30/15 12:24 PM (8 years, 1 month ago)

Great,

I'll grab some plates tonight and give it a shot with my 2000ml fat fuck :lmafo:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: taGyo]
    #22594493 - 11/30/15 12:26 PM (8 years, 1 month ago)

yea I wouldn't use one that size myself but you know, you got gyo hands


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OfflinetaGyo
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Re: pour agar TEK [Re: bodhisatta]
    #22594499 - 11/30/15 12:27 PM (8 years, 1 month ago)

Skinny, tall black guy with dreads and all :lmafo:


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: taGyo]
    #22594559 - 11/30/15 12:42 PM (8 years, 1 month ago)


I've been toying with BRF agar lately but it's still too nutritious for these plates. should have used food coloring too it's really hard to see. the golden halo plate is grown to the edge but you can't really tell in the picture. envyss refuses to go tomentose on all of the BRF plates.


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OfflinetaGyo
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Re: pour agar TEK [Re: bodhisatta]
    #22594618 - 11/30/15 12:56 PM (8 years, 1 month ago)

That ESS looks aggressive!

Yeah I was gonna ask if you food color any of your plates 'cause I'm blind as a bat, if it's not white on black I'm fucked :lmafo:

Why do you say too nutritious? I don't mind tomentose or slower growth, maybe it's personal preference.


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: taGyo]
    #22594643 - 11/30/15 01:04 PM (8 years, 1 month ago)

I don't mind it either but they're not the most pretty


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OfflinecronicrFacebook
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Re: pour agar TEK [Re: bodhisatta]
    #22594733 - 11/30/15 01:32 PM (8 years, 1 month ago)

bod remind me of this thread in a couple days, would make a great sticky:smile:


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