Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Left Coast Kratom Kratom Powder For Sale   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: < First | < Back | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | Next >
OfflineCyberoxide
Stranger
Male

Registered: 10/25/19
Posts: 7
Last seen: 1 year, 10 months
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26347354 - 11/26/19 06:48 PM (4 years, 2 months ago)

Found out my answer the hard way lol


Extras: Filter Print Post Top
OfflineJooby
Stranger
Male


Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
Re: pour agar TEK [Re: Cyberoxide]
    #26353619 - 11/30/19 11:02 AM (4 years, 1 month ago)

Recipe for MEA states 20g/L so if I am following instructions & filling media bottle to 400ml...

8g LME
8g Agar
400ml h2o

Correct?


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: pour agar TEK [Re: Jooby]
    #26353621 - 11/30/19 11:02 AM (4 years, 1 month ago)

Yep


Extras: Filter Print Post Top
OfflineJooby
Stranger
Male


Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
Re: pour agar TEK [Re: bodhisatta]
    #26353684 - 11/30/19 11:35 AM (4 years, 1 month ago)

Ty


Extras: Filter Print Post Top
InvisibleWhyterye
Male


Registered: 02/23/18
Posts: 1,218
Loc: Colorado Flag
Re: pour agar TEK [Re: Jooby]
    #26377309 - 12/12/19 02:06 PM (4 years, 1 month ago)

So you microwave  the bottle for a shortcut instead of boiling the agar and nutes? Sorry for the noob question


Extras: Filter Print Post Top
Offlineheavy
Stranger
Male

Registered: 12/14/19
Posts: 4
Loc: Minnesota
Last seen: 3 years, 7 months
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26395372 - 12/22/19 10:35 AM (4 years, 1 month ago)

:heart:


Extras: Filter Print Post Top
InvisibleCosmicGiggle
Aspiring


Registered: 11/19/19
Posts: 265
Re: BOD's Comprehensive Agar TEK [Re: heavy]
    #26422945 - 01/08/20 01:10 PM (4 years, 22 days ago)

So Bod I followed your directions and set my agar jar in a 120F water bath after taking it out of the pressure cooker, water bath rose to about 125 then when it dropped back down (I waited a little too long and accidentally let the water bath get down to about 116f) I took the agar jar out and put it in the SAB to start pouring. I still got pretty bad condensation and I literally couldn’t have let the agar get any cooler because by the last plate (I only poured 9) the agar was coming out lumpy/solidified.

Did I miss something? I will try the hot cup of water on top of the petri before I wrap them next time. I tried that this time but only AFTER I wrapped with parafilm and the hot cup of water melted the parafilm on top of the petri. It did seem to work decently. Didn’t really get rid of the condensation, but it consolidates it into fewer big drops instead of hundreds of teeny tiny droplets so it did make it easier to see through.


--------------------


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: CosmicGiggle]
    #26422959 - 01/08/20 01:16 PM (4 years, 22 days ago)

Did you let them cool and solidify in a stack?


Extras: Filter Print Post Top
InvisibleCosmicGiggle
Aspiring


Registered: 11/19/19
Posts: 265
Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #26423056 - 01/08/20 02:19 PM (4 years, 22 days ago)

Quote:

verum subsequentis said:
Did you let them cool and solidify in a stack?




Yes, they sat stacked in the SAB for about 30 min until I finally put them back in the bag they came in and threw eM in the closet.  Only 2 are condensation free of the 8 plates I have left (one got cracked) 4 of them have too much condensation to see into and another 2 have moderate condensation, not enough to call nearly opaque like the other 4 but still too much to tell what’s really going on inside.

It’s not a huge deal right now because I’m not using food coloring or anything so I can see into them from the bottom.  But soon I would like to start using recipes that call for stuff that will make the agar less clear but I would like to get the condensation tamed down before I start using different recipes. I guess because it’s such a small amount of dishes? If I pour more plates at a time and make bigger stacks of them then less of them will have condensation, right?


--------------------


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: CosmicGiggle]
    #26423150 - 01/08/20 03:15 PM (4 years, 22 days ago)

a stack of nine should do alright. I leave to cool in stacks of ten and i do fine. Maybe the room you poured in was really cold. I don't know. It's odd.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #26423193 - 01/08/20 03:45 PM (4 years, 22 days ago)

I had the cold room problem last time I poured.

Usually only the top of the stack is wet but this time besides the normal condensation on the top plates all the other ones had a white circle of mist that looked like a fogged up car window, and it got smaller each plate down.

I’ve been either leaving the bad ones unwrapped in the box hoping they dry or propping them up on their sides for a day so it all pools up and then dumping it out in the SAB.

I got a little over zealous trying to shake a drop off earlier and the whole agar circle fell out lol.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleCosmicGiggle
Aspiring


Registered: 11/19/19
Posts: 265
Re: BOD's Comprehensive Agar TEK [Re: A.k.a]
    #26423220 - 01/08/20 03:59 PM (4 years, 22 days ago)

My apartment is kept at 68 during the day, 66 at night. It was probably 67-68F when I poured. I read someone saying to store them upside down. Which I did for a couple days. Then I was reading in the archives today and came across a post by RR that said not to do that with an explanation as to why. Gonna have to start setting a hard rule to not follow anyone’s advice without a TC under their name. I know there’s people that know what they speak that don’t have the tag but there’s way for a noob to know who’s who and who knows what.:shrug:


--------------------


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: CosmicGiggle]
    #26423323 - 01/08/20 05:19 PM (4 years, 22 days ago)

what was the reason to not store them upside down?

Only thing I can think of would be then the water would pool up on the agar instead of the lid.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: A.k.a] * 1
    #26423465 - 01/08/20 07:09 PM (4 years, 22 days ago)

Lots of highly experienced people have idiosyncratic reasons for doing or not doing things. And RR is no exception. He is an innovator and a legend but still human and therefore occasionally fallible. Take it all with a grain of salt but if someone with a recent sign up date and no ratings disagrees with a TC its probably wrong. But someone who’s been doing it for a long time with lots of good ratings can still give grwat advice, and some disagreements are based personalilty difference, preference or idiosyncrasies. 


I would never store unwrapped plates upside down. But after they are good and sealed I see absolutely no reason not to:shrug:


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
🅂 🄰 🄼 🄴  🅃 🄴 🄰 🄼
🅃 🄴 🄰 🄼  🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Extras: Filter Print Post Top
InvisibleCosmicGiggle
Aspiring


Registered: 11/19/19
Posts: 265
Re: BOD's Comprehensive Agar TEK [Re: A.k.a]
    #26424039 - 01/09/20 06:59 AM (4 years, 21 days ago)

Quote:

A.k.a said:
what was the reason to not store them upside down?

Only thing I can think of would be then the water would pool up on the agar instead of the lid.




Why not to store upside down by RR

Not sure where the original post is that bodhi was quoting in there. I actually found that post by searching for the word “condensation” posted by bodhi because I knew he has some advice about it somewhere in this thread but I didn’t wanna search through every page again. That thread was the first thing that popped up and I found it even more helpful than what I was looking for.


--------------------


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: CosmicGiggle]
    #26424358 - 01/09/20 10:39 AM (4 years, 21 days ago)

Oh cool thanks I’m curious what he says.


--------------------
LAGM2020


Extras: Filter Print Post Top
OfflineCj69420
Stranger
Registered: 01/13/20
Posts: 1
Last seen: 3 years, 11 months
Re: BOD's Comprehensive Agar TEK [Re: A.k.a]
    #26432896 - 01/14/20 09:57 AM (4 years, 16 days ago)

Thanks for the TEK. I’m new to all of this and don’t know much about mycology or chemistry so I have a lot to learn. I think I might have misread your tek and ordered to many ingredients.

You put: “ I personally like to use, in give or take this order
Grain Soak Water agar(save water from hydration of grain spawn add 20g/L Agar-Agar powder)
BRF agar (20g/L BRF and Agar-Agar)
MEA, malt extract agar. Use light malt extract for homebrewing at 20g/L
YPD, yeast peptone dextrose agar, I buy this premade and use as is
PDA, I just kind of wing this one every time I use it”

I misread that as ingredients to all mix together to make a batch of agar but now realize these are 5 different recipes right? I also want to clarify, the Grain Soak Water agar is literally just water saved from hydrating the grain spawn mixed with Telephone agar-agar powder? That’s it? It’s that easy? Just the water and powder? I feel like I’m overthinking everything so if you can clear it up I’d appreciate it. Thanks for the awesome tek!


Edited by Cj69420 (01/14/20 09:59 AM)


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: Cj69420]
    #26433402 - 01/14/20 03:01 PM (4 years, 16 days ago)

Yes that easy


Extras: Filter Print Post Top
InvisibleCosmicGiggle
Aspiring


Registered: 11/19/19
Posts: 265
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26441285 - 01/19/20 07:57 AM (4 years, 11 days ago)

Anyone have tips/tricks for cleaning the media bottle after pouring? Found it a little hard to get all the hardened agar bits stuck to the inside of the bottle. Surely someone here has figured out a really quick or simple way to clean them


--------------------


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: CosmicGiggle]
    #26441306 - 01/19/20 08:25 AM (4 years, 11 days ago)

I’ve alway just left it until the next time.

I did have mold growing in the bottle for the first time last week, so I squirted some dish soap in then filled it a third up with water shook it real well and let it sit all day, cleaned everything out.

Besides that filling it with water and pcing or boiling it should work.


--------------------
LAGM2020


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | Next >

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Left Coast Kratom Kratom Powder For Sale   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* Agar Tek Aristar 5,815 12 08/01/03 06:42 PM
by shirley knott
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,732 8 12/13/19 06:16 PM
by mushboy
* Re: Peroxidated Agar Tek sparks 4,556 6 05/08/00 12:43 AM
by Anonymous
* agar tek? the man 2,773 4 12/16/02 10:45 AM
by the man
* Agar ;)
( 1 2 all )
SCIOpenEyedDream 7,182 25 09/01/04 09:58 AM
by fastfred
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,285 38 05/11/01 11:08 AM
by MAIA
* Mycelium-only tek, Please Help! FaminedShaman 2,563 4 05/06/01 06:49 AM
by egghead
* Antibac Agar, DIY! superduper 4,846 13 12/04/02 02:20 PM
by superduper

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
641,013 topic views. 41 members, 311 guests and 47 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.009 seconds on 13 queries.