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OfflineCapitainFrog
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Registered: 06/25/19
Posts: 43
Loc: Zone 6-7 Flag
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Re: pour agar TEK [Re: CapitainFrog]
    #26074982 - 06/26/19 12:04 PM (4 years, 7 months ago)

Thank you, do you have any glass? Or PC the plastic?


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Offlineacobs90
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Registered: 05/17/19
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Last seen: 4 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26075021 - 06/26/19 12:25 PM (4 years, 7 months ago)

Thank you, awesome TEK BOD! Is there a time limit on the grain soak water? I made way too much and have it stored in the fridge until I have a chance to make more agar.


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: acobs90]
    #26075059 - 06/26/19 12:47 PM (4 years, 7 months ago)

Glass ones are a pain to work with and usually a few bucks a piece is a good deal.


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Offlineohfunkya
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I'm a teapot

Registered: 01/20/19
Posts: 72
Loc: Canada Flag
Last seen: 2 years, 11 months
Re: pour agar TEK [Re: bodhisatta]
    #26075069 - 06/26/19 12:54 PM (4 years, 7 months ago)

I’ve had great success with your tek, thanks! Also recommended to a friend who failed with every previous recipe he tried, and he’s had great results with yours!


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OfflineCapitainFrog
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Registered: 06/25/19
Posts: 43
Loc: Zone 6-7 Flag
Last seen: 1 year, 3 months
Re: pour agar TEK [Re: ohfunkya]
    #26075260 - 06/26/19 03:11 PM (4 years, 7 months ago)

http://bioland-sci.com/index.php?main_page=product_info&products_id=1279
$0.13 is cheap enough to not mess with them, here’s the link for $65 case of 500 I found. Sorry for the annoyance


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OfflineTruffleRuffler
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Registered: 06/16/19
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Re: pour agar TEK [Re: CapitainFrog]
    #26076775 - 06/27/19 10:22 AM (4 years, 7 months ago)

Should/can dishes be stored in stacks after inoculation?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: TruffleRuffler] * 1
    #26076810 - 06/27/19 10:35 AM (4 years, 7 months ago)

Yes, kind of the only way to store them unless you have a lot of tables


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OfflineTruffleRuffler
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Re: pour agar TEK [Re: bodhisatta]
    #26076956 - 06/27/19 12:10 PM (4 years, 7 months ago)

Quote:

bodhisatta said:
Yes, kind of the only way to store them unless you have a lot of tables




True, makes sense. I just want to be sure before I effed something up lol


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Invisiblefunky123
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Registered: 08/27/17
Posts: 257
Loc: Austria
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26093385 - 07/06/19 03:34 PM (4 years, 6 months ago)



Quote:

then place into your pressure cooker, I like to make the water in the PC come up to the level of the agar in the bottle. this will make it take longer to hit pressure and cool down but will cut down on boil over.




I understand this as filling the PC with water up to level C. Is that correct? Or rather B? Intuitively I would have filled up to A or even a bit lower.


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: funky123]
    #26093439 - 07/06/19 04:21 PM (4 years, 6 months ago)

Between b and c including all the way up to c


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Offlineenamored
I'm a teapot
Registered: 06/25/19
Posts: 51
Last seen: 2 months, 17 days
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26096150 - 07/08/19 01:02 PM (4 years, 6 months ago)

this whole easy AF series has been a gamechanger

thanks homie  :rockon:


Edited by enamored (07/09/19 03:52 PM)


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OfflineFryMe
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Registered: 01/11/19
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Last seen: 4 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: enamored]
    #26110165 - 07/15/19 05:35 PM (4 years, 6 months ago)

Posted in another thread, but I've been doing heaps of bulk grows with unmod tubs and spore syringe to jars, all with great success and no contams beyond a few trich situations when the heat got to much in the room after a bunch of flushes. So I decided to try out some Pan Cy's and thought, hey let's try out some agar plates this time.

Used needle to agar plate via these methods and all my plates went mold on me within 4 days. I'm heaps particular on sterile environments in my SAB, yet still got contam. Day one the dishes had a bunch of white circles starting and then the next morning were all grey spots that only got darker. I just tried some new dishes to see if maybe it was just a fluke. Any thoughts? Followed all the instructions well, didn't know if maybe that strain is just hard to start in agar possibly.


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OfflineMushroom Person
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Registered: 06/28/19
Posts: 15
Last seen: 1 year, 7 months
Re: BOD's Comprehensive Agar TEK [Re: FryMe]
    #26121772 - 07/21/19 12:28 PM (4 years, 6 months ago)

Thanks for all the great work, Boddhisatta, I learned a lot from your threads. I have 20 petri dishes and I'm wondering how to best use them. How many should I inoculate right away, and how many should I save for transfers? Also, should I fill them all with agar right away, and store the rest wrapped in saran wrap? Basically, fill all with agar, inocululate about five, and save the rest?


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: BOD's Comprehensive Agar TEK [Re: Mushroom Person]
    #26121975 - 07/21/19 02:23 PM (4 years, 6 months ago)

That sounds like a solid plan to me!


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InvisibleFunky Monkey
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Registered: 05/14/19
Posts: 1,099
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26122349 - 07/21/19 05:55 PM (4 years, 6 months ago)

POST DELTED

That guy got banned so let's get back to keeping it positive!

Carrying on :thumbup:


Edited by Funky Monkey (07/21/19 10:52 PM)


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OfflineGwindor
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Registered: 05/31/19
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Loc: Russia
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26153128 - 08/26/19 02:14 AM (4 years, 5 months ago)

Do I need to use glass petri dishes?
Or is it ok to use plastic petri dishes?
Could you provide links to amazon to proper dishes please?


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Offlinejbgtaa
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Registered: 06/09/19
Posts: 1,785
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Re: BOD's Comprehensive Agar TEK [Re: Gwindor]
    #26153137 - 08/26/19 02:25 AM (4 years, 5 months ago)

Quote:

Gwindor said:
Do I need to use glass petri dishes?
Or is it ok to use plastic petri dishes?
Could you provide links to amazon to proper dishes please?




Some use glass but buying petri dishes in bulk and either premixed MEA or PDA (dehydrated) by the 100g brings the cost of agar work down to the disposable level. 100 bucks for a year+ supply of agar plates makes it alot easier to just saran wrap them and throw them away when the contam or break or whatever.
https://www.amazon.com/Celltreat-229694-Petri-Slideable-Sterile/dp/B014AFZ820/ref=sr_1_2?keywords=500+celltreat+petri+dishes&qid=1566807738&s=gateway&sr=8-2


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Edited by jbgtaa (08/26/19 02:26 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: Gwindor]
    #26153208 - 08/26/19 05:03 AM (4 years, 5 months ago)

Quote:

Gwindor said:
Do I need to use glass petri dishes?
Or is it ok to use plastic petri dishes?
Could you provide links to amazon to proper dishes please?



Read the first post :thumbup:


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OfflineGnomemush
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Registered: 07/14/19
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Last seen: 3 years, 14 days
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26159452 - 08/29/19 05:59 PM (4 years, 4 months ago)

does anyone know how much organic dry malt extract to use?


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: Gnomemush]
    #26159523 - 08/29/19 06:44 PM (4 years, 4 months ago)

2% works we for malt


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