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OfflineFrank Rizzo
Old whipper snapper


Registered: 06/12/18
Posts: 184
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Re: pour agar TEK [Re: Spreadtheknowledge]
    #25975415 - 05/06/19 10:01 AM (4 years, 8 months ago)

Sorry if this is a stupid question but can't seem to find the answer.  If made some agar plates and slants at the same time but didn't want to use the slants right away as I want to work out some clean samples on the plates first how long can I store the slants before I use them?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: Frank Rizzo]
    #25975526 - 05/06/19 10:47 AM (4 years, 8 months ago)

Pretty much indefinitely. But i would personally make new ones if i hadn't inoculated them within a month or two


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OfflineFrank Rizzo
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Registered: 06/12/18
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Re: pour agar TEK [Re: bodhisatta]
    #25975580 - 05/06/19 11:18 AM (4 years, 8 months ago)

Thanks


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OfflineiEnter
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Registered: 03/17/16
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25981288 - 05/09/19 06:16 AM (4 years, 8 months ago)

In BOD we trust!


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InvisibleLegionOfShroom
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Registered: 02/13/17
Posts: 235
Re: BOD's Comprehensive Agar TEK [Re: iEnter]
    #25988696 - 05/12/19 08:18 PM (4 years, 8 months ago)

Do you have to crack the lid of the media bottles before reheating in the microwave, or can you leave them sealed?


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: LegionOfShroom]
    #25988703 - 05/12/19 08:22 PM (4 years, 8 months ago)

Id crack it


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InvisibleLegionOfShroom
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Registered: 02/13/17
Posts: 235
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25988801 - 05/12/19 09:04 PM (4 years, 8 months ago)

Thanks BOD! :psychsplit:


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OfflineZovers
Messy Burger
I'm a teapot

Registered: 12/15/17
Posts: 61
Last seen: 4 years, 7 months
Re: pour agar TEK [Re: LegionOfShroom]
    #26016051 - 05/27/19 11:23 AM (4 years, 8 months ago)

Excellent guide, very detailed and informative! I am about to start my first grain jars and boy am I glad I found this prior to that! I will definitely be trying this very soon and am looking forward to this next step in my journey through mycology. Thank you!


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OfflineShroomterd
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Registered: 06/28/17
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Loc: washington
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26024095 - 05/30/19 11:46 PM (4 years, 7 months ago)

Thanks a lot this really helped mine is cooling as I type .. I think everything has gone well so far it took awhile for me to find a recipe for mea I know it’s simple for most but there seemed to be a lot of conflict on the topic I went with 8 grams agar 15 malt extract and 500 ml water if anyone is wondering.. thanks again


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: Shroomterd]
    #26024282 - 05/31/19 05:11 AM (4 years, 7 months ago)

1-4% nutrients w/v% works great for most agar anything. I like to stick around 2% with malt


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OfflineKing0fthajuice
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Registered: 06/28/17
Posts: 325
Loc: Wild Like Volusia in the 80s
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26024420 - 05/31/19 07:49 AM (4 years, 7 months ago)

Quote:

bodhisatta said:
Id crack it




I’m glad this was asked because I never saw it in your tek, I actually just put a half bottle of agar in the fridge yesterday and was thinking I’d have to reread the whole thread and then bam right in the kisser


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Offlinelilwoman
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Registered: 05/03/19
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Re: BOD's Comprehensive Agar TEK [Re: King0fthajuice]
    #26042117 - 06/09/19 03:52 PM (4 years, 7 months ago)

Success EVERY TIME! This is an absolute God-send for this ultra-noob!!! ❤️


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Offlinejbgtaa
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Registered: 06/09/19
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26047074 - 06/11/19 11:09 PM (4 years, 7 months ago)

hey i just got one question: where do you buy your plastic petris? I can only find polystyrene ones on amazon, and as far as i know those can;t be pressure cooked.


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: BOD's Comprehensive Agar TEK [Re: jbgtaa]
    #26047293 - 06/12/19 04:12 AM (4 years, 7 months ago)

Type celltreat petris into Amazon.


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Offlineverum subsequentis
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Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #26047297 - 06/12/19 04:14 AM (4 years, 7 months ago)

Oops.... Wait wait wait. You can't cook any plastic petris. You aren't supposed to. You cook the agar and then pour it into the petris. They come sterile.


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Invisibletryptonite
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Registered: 02/06/04
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Re: pour agar TEK [Re: PurePleasure]
    #26047319 - 06/12/19 04:38 AM (4 years, 7 months ago)

I just want to add this because I make heaps of agar (day job)

Instead of microwaving to boil in the schott bottle I stir the agar mixture solution into an open plastic jug. I chuck 2 jugs of 500mL (half a quart??? two cups?? sorry I don't know what that is in gallons) of agar in the microwave for 10 minutes. Stop every 2 minutes to stir, usually it will start to boil in the 8th or 9th minute when it does stop it and give it a good stir. Then put it back in the microwave and when it boils again take it and stir it one last time. By now it should be fully dissolved, transfer to your schott bottle and autoclave or PC as normal.

Only thing is I use pre-made agar mixtures. Results may vary with your own agar mixture but it will boil much quicker this way as the schott bottles are made from thick pyrex so heat takes a while to penetrate. Also the plastic jugs are 1 Liter (quart, 4 cups, 1/4 gallon??) to allow space for the bubbles to rise and not boil over out of the jug. This way if your late to take it out the microwave there is heaps of headspace so you have plenty of time before it boils over and makes a mess


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Offlinejbgtaa
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Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #26047950 - 06/12/19 11:51 AM (4 years, 7 months ago)

Quote:

verum subsequentis said:
Oops.... Wait wait wait. You can't cook any plastic petris. You aren't supposed to. You cook the agar and then pour it into the petris. They come sterile.



thats what i was thinking. after reading all the agar teks i realized they werent forgetting to pressure cook them, it's just not necessary as long as the agar itself is sterile.


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If the thunder don't get ya, the lightning will.
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OfflineCapitainFrog
Leeroy

Registered: 06/25/19
Posts: 43
Loc: Zone 6-7 Flag
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Re: pour agar TEK [Re: jbgtaa]
    #26074823 - 06/26/19 10:26 AM (4 years, 7 months ago)

Where are you getting bulk plates? Even on Amazon the plastic are more expensive than what you’ve posted.


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: CapitainFrog]
    #26074919 - 06/26/19 11:16 AM (4 years, 7 months ago)

I google for best deal. Usually amazon find 500 for around 80$


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OfflineCapitainFrog
Leeroy

Registered: 06/25/19
Posts: 43
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Re: pour agar TEK [Re: CapitainFrog]
    #26074979 - 06/26/19 12:03 PM (4 years, 7 months ago)

Well I just saw verums post but was curious about glass dishes that are affordable, or I’ll just have to piece them together over time...


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