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whitewombat
Ancient Alien

Registered: 12/03/18
Posts: 50
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Re: BOD's Comprehensive Agar TEK [Re: jamslam]
#25661990 - 12/06/18 03:42 PM (5 years, 1 month ago) |
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Thanks Bod!
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In Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
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Re: BOD's Comprehensive Agar TEK [Re: whitewombat]
#25679588 - 12/14/18 06:18 PM (5 years, 1 month ago) |
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Firstly thanks to the wise masters for helping us noobers get up and running. Going to pour some plates soon.
RR says he puts yeast in his MEA mix but then he says he uses a premix.
I am seeing 15 grams Malt Extract, 10 grams Agar in 500ml water as well as a 10/10 ratio. What's the consensus on this?
Is yeast necessary?
Is distilled water better than tap?
My tap water is pretty foul tasting. I drink bottled spring water.
-------------------- I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!
Edited by In Lakech (12/14/18 06:31 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: BOD's Comprehensive Agar TEK [Re: In Lakech]
#25679642 - 12/14/18 06:43 PM (5 years, 1 month ago) |
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malt is fine on its own. use the bottled water then. tap is fine.
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In Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25679934 - 12/14/18 10:10 PM (5 years, 1 month ago) |
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Thanks man. I appreciate it.
-------------------- I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!
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Mondangle
Noble Savage

Registered: 10/22/18
Posts: 73
Last seen: 2 years, 9 months
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25727052 - 01/06/19 11:48 AM (5 years, 24 days ago) |
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First off, thank you Bod, you've been a huge help getting me started.
So,
I did enlightments TLDR method that you posted, using 500ml water, 10g malt extract and 10g agar, and it wouldn't get foamy when whisking it all together. Is that okay?
-------------------- Self image = Success Expectations
Edited by Mondangle (01/06/19 11:51 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: BOD's Comprehensive Agar TEK [Re: Mondangle]
#25727213 - 01/06/19 01:01 PM (5 years, 24 days ago) |
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Foamy is generally a bad thing with agar
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Mondangle
Noble Savage

Registered: 10/22/18
Posts: 73
Last seen: 2 years, 9 months
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25730135 - 01/07/19 08:13 PM (5 years, 22 days ago) |
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I Brought the tempterature of the agar down to 120F exactly but when pouring, it all came out lumpy. Is this a deal breaker? I think I just didn't reheat it enough before putting in the bath... though it was really hot and I thought I got all the lumps out.
I did the 10g malt 10g agar 500ml recipe.
-------------------- Self image = Success Expectations
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: BOD's Comprehensive Agar TEK [Re: Mondangle]
#25730163 - 01/07/19 08:20 PM (5 years, 22 days ago) |
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To re melt agar takes near boiling. Look up agar hysteresis in the tek.
Your thermometer isn't calibrated or you went too slow is the reason for lumps.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25730169 - 01/07/19 08:23 PM (5 years, 22 days ago) |
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One time I had grain water in a mason jar in the fridge and home made apple pie liquor in a mason jar in the fridge. And my wife didn't know. They looked similar enough. I mean I could easily tell the difference but she didn't even know there was anything else in the fridge that wasn't apple pie. Well she loaded up two shot glasses and down the hatch the nastiest shit that ever got drank in my life. Extremely disappointing flavor.
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Mondangle
Noble Savage

Registered: 10/22/18
Posts: 73
Last seen: 2 years, 9 months
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25732033 - 01/08/19 04:54 PM (5 years, 21 days ago) |
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lol. That reminds me when my aunt thought there was a bunch of water in the cooler... but it was moonshine. She was dehydrated after a jog and chugged a little of it before realizing haha!
And thanks again for the help man. I've read all your guides. This is my first go. Made mini unmodded monos with only coir for the 4 half pints that colonized from lc, each half pint is its own mono, in case of bad spawn... got the agar ready with mycelium in it... feeling pretty confident.
-------------------- Self image = Success Expectations
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CreamyGoodness
Lifter of things



Registered: 12/11/18
Posts: 94
Last seen: 3 years, 9 months
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Re: BOD's Comprehensive Agar TEK [Re: Mondangle] 1
#25736758 - 01/10/19 06:47 PM (5 years, 19 days ago) |
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I PC'd a media bottle of agar but didn't use it, its sitting in the fridge. I'm about to microwave it to get it ready again. Do I need to loosen this cap a bit before microwaving it? I'm concerned if I don't loosen it, it could build up too much pressure. If I loosen the cap, I'm afraid it might try to suck in air since it was tightened while it was hot. Whats the correct way?
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newtomyc
enthusiast


Registered: 12/24/18
Posts: 1,038
Loc: here there and everywhere
Last seen: 8 months, 15 days
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25778911 - 01/30/19 11:15 AM (5 years, 4 hours ago) |
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On fixing the plates with condensation, do you allow Agar to set and then use the cup?
JJ
-------------------- JJ Draw unto others as they have been drawn to you.... WSP
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el_toro

Registered: 01/17/19
Posts: 54
Last seen: 10 months, 28 days
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25800662 - 02/09/19 04:00 AM (4 years, 11 months ago) |
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It's amazing what I've managed to learn in a short period of time in these forums. Thanks Bod for the write up!
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kurshiukas
Stranger


Registered: 02/02/10
Posts: 219
Loc: LTU
Last seen: 4 years, 10 months
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Re: BOD's Comprehensive Agar TEK [Re: el_toro]
#25826716 - 02/21/19 04:31 AM (4 years, 11 months ago) |
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A question - is it necessary to boil everything before sterilization?. I think I have seen some suggestions on using premixes (that I believe are just 50/50 mixes of agar and LME) where it is suggested just to soak the mix cold for some time, them pour in to media bottle and down in to pressure cooker for 20 min.
So far though - all my dishes are clean of anything - nothing grown on them for like 2-3 weeks (although I am using very old vials). I am thinking that maybe I have over boiled sugars in the media and its not that yummy for spores to propagate?
Also, how and when do you filter agar so its clean of any sediments?
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gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
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Re: BOD's Comprehensive Agar TEK [Re: kurshiukas]
#25827260 - 02/21/19 09:29 AM (4 years, 11 months ago) |
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If you want to filter it I suppose cheesecloth is fine. I don't know much about premixed agar but I suppose that is something you're buying from an external source. If so maybe they suggest that because of settling due to it not being prepared fresh by your doing. For anything else, if you're following this guide anything outside of the guide isn't required or else it would be stated in the guide. Required steps are found in the guide.
-------------------- One must be mindful to maintain their balance on the slippery earth. Bod's Comprehensive Agar Resource Gizmodo's Market Stall Say No To Grow Kits
Edited by gizmodo (02/21/19 09:30 AM)
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lost wanderer
Stranger

Registered: 09/24/18
Posts: 40
Last seen: 2 months, 21 days
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Re: BOD's Comprehensive Agar TEK [Re: gizmodo]
#25829626 - 02/22/19 09:17 AM (4 years, 11 months ago) |
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thanks Bod. Your teks have gotten me far in this hobby.
I learned a lesson I should have already figured out when pouring my first plates......... Make sure your table is level 
I also had a question for you i have not seen answered. Are you left or right handed?
I have been watching your videos and I think you are left handed. It will help with my mental visualization of my own procedures.
Also, I do not understand how the cup trick removes the condensation of the dishes? Where does the humidity go? Should i do it before i wrap them?
Thanks
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: BOD's Comprehensive Agar TEK [Re: kurshiukas]
#25829630 - 02/22/19 09:19 AM (4 years, 11 months ago) |
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Quote:
CreamyGoodness said: I PC'd a media bottle of agar but didn't use it, its sitting in the fridge. I'm about to microwave it to get it ready again. Do I need to loosen this cap a bit before microwaving it? I'm concerned if I don't loosen it, it could build up too much pressure. If I loosen the cap, I'm afraid it might try to suck in air since it was tightened while it was hot. Whats the correct way?
Loosen. Tightened to shake it otherwise yea...
Quote:
newtomyc said: On fixing the plates with condensation, do you allow Agar to set and then use the cup?
JJ
Yes
Quote:
kurshiukas said: A question - is it necessary to boil everything before sterilization?. I think I have seen some suggestions on using premixes (that I believe are just 50/50 mixes of agar and LME) where it is suggested just to soak the mix cold for some time, them pour in to media bottle and down in to pressure cooker for 20 min.
So far though - all my dishes are clean of anything - nothing grown on them for like 2-3 weeks (although I am using very old vials). I am thinking that maybe I have over boiled sugars in the media and its not that yummy for spores to propagate?
Also, how and when do you filter agar so its clean of any sediments?
Necessary no, but neither is wearing a seatbelt to drive. I personally consider it necessary despite it not actually being necessary if that makes sense. My microwave can boil it up in 3-5 minutes seems stupid to skip making sure you gave a homogenized mix before sterilization and removes dissolved oxygen
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
lost wanderer said: thanks Bod. Your teks have gotten me far in this hobby.
I learned a lesson I should have already figured out when pouring my first plates......... Make sure your table is level 
I also had a question for you i have not seen answered. Are you left or right handed?
I have been watching your videos and I think you are left handed. It will help with my mental visualization of my own procedures.
Also, I do not understand how the cup trick removes the condensation of the dishes? Where does the humidity go? Should i do it before i wrap them?
Thanks
Left handed. The condensation evaporates and recondenses on the agar rather than the lid. When the plate was poured the lid was cooler than the agar condensation appears on relatively cooler surfaces first. When u do hot cup the agar is cooler than the lid the condensate forms on the agar instead. Then is reabsorbed usually
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Superchang
Stranger

Registered: 02/04/19
Posts: 20
Last seen: 2 years, 1 month
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so, like - what about just transfering a spore print from foil to premade agar?
can I just heat the paperclip, dip in the premade agar plate, run it across the foil, then zig-zag across the premade agar plate?
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Cthulhu23
Elder God Extraordinaire



Registered: 07/04/18
Posts: 620
Last seen: 2 years, 6 months
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I personally prefer using sterile cotton swabs (I have better luck with those than with a wire loop). Shouldn’t be any need to run it across the Agar first though - heat your paperclip (in a loop, preferably) until red hot, let cool a little, swipe through spore print, then zig-zag across agar.
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