|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
DirdyD


Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 8 days
|
|
Awesome. Would a microscope be useful or even practical for identifying possible contaminations on agar? Ive heard it wouldnt.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: pour agar TEK [Re: DirdyD] 1
#22118476 - 08/20/15 08:31 PM (8 years, 5 months ago) |
|
|
It's what I use to identify or help do so with contamination on agar
|
Trek222
Trek222



Registered: 06/25/15
Posts: 67
|
|
Hey Bodhi,
Look what I got in the mail today. All because of your Pour Agar Tek.

I'm so pleased you recommended these Pyrex media bottles. The marketing literature states that they can be used for storage as well as mixing and sampling. They include a one-piece autoclavable orange polypropylene plug seal GL45 threaded cap with a drip-free pouring ring.
I've used a sleeve of dishes to practice pouring. So far so good. I will be pouring my first plates in the near future.
Its also going to be my first time using my new AA921.

Thanks again.
Edited by Trek222 (08/28/15 06:58 AM)
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: pour agar TEK [Re: Trek222]
#22155136 - 08/28/15 07:03 AM (8 years, 5 months ago) |
|
|
 Let's start am uncomplicated agar revolution
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: pour agar TEK [Re: Trek222] 1
#22155169 - 08/28/15 07:16 AM (8 years, 5 months ago) |
|
|
Quote:
Trek222 said: 
Dude.... Your AA921 Gauge is broken!
|
Trek222
Trek222



Registered: 06/25/15
Posts: 67
|
Re: pour agar TEK [Re: Juiceh]
#22155277 - 08/28/15 08:03 AM (8 years, 5 months ago) |
|
|
I've taken a picture of the broken pressure regulator gauge and will be contacting the company as the PC was never used. The styrofoam packing surrounding the gauge was cracked.
I will not use the PC until I get a reply from the company. The gauge itself is $20.00 on Amazon.
However, don't want to pony up the money for a new one because I did not damage the gauge.
Hopefully, the company will eat the cost and send me a new one. Good eye Juiceh.
Edited by Trek222 (08/31/15 11:01 AM)
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: pour agar TEK [Re: Trek222]
#22155305 - 08/28/15 08:13 AM (8 years, 5 months ago) |
|
|
|
DirdyD


Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 8 days
|
|
Quote:
bodhisatta said:
 Let's start am uncomplicated agar revolution
What would this consist of? Sounds awesome.
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: pour agar TEK [Re: DirdyD]
#22155406 - 08/28/15 08:51 AM (8 years, 5 months ago) |
|
|
It consists of reading this tek and following it.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: pour agar TEK [Re: Juiceh]
#22156105 - 08/28/15 11:21 AM (8 years, 5 months ago) |
|
|
agar is easy people complicate it thinking they need all the extras and fancy stuff
|
tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 3 hours, 32 minutes
|
|
|
blackdust


Registered: 02/28/09
Posts: 8,327
|
|
could just use a liquor bottle per RR
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
It's less about the bottle, there's 100s you could use. I do enough agar I got the bottle I like. Making a filter lid is unnecessary though
|
Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
|
|
Yupp, Filtered lids are for "no-pour"
-------------------- Giving is all we have, for we're just grateful to be alive

|
blackdust


Registered: 02/28/09
Posts: 8,327
|
|
Quote:
bodhisatta said: It's less about the bottle, there's 100s you could use. I do enough agar I got the bottle I like. Making a filter lid is unnecessary though
did not know that. Cool!
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
|
Quote:
bodhisatta said: It's less about the bottle, there's 100s you could use. I do enough agar I got the bottle I like. Making a filter lid is unnecessary though
Yup. Some people even use qt jars.
Pouring a few sleeves tonight. I got 12 more species to play with! Woo Wooooo! Media bottles.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: pour agar TEK [Re: Juiceh]
#22158039 - 08/28/15 07:13 PM (8 years, 4 months ago) |
|
|
I just made some charcoal brf agar for two sleeves worth a little over 800mL
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
|
|
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,854
Last seen: 15 hours, 56 minutes
|
Re: pour agar TEK [Re: taGyo]
#22222089 - 09/11/15 01:46 PM (8 years, 4 months ago) |
|
|
Quote:
sterilize your agar for 10-15m at 15PSI. once your PC is back at atmospheric pressure. open it up take your jars out. put them in 47ºC water bath. your agar jars are hotter than 47 so the water bath will warm up. once it cools back down to 47ºC then you're ready to pour.
I just want to add it's important to filter any solids out of your agar if you're using very short sterilization times. Those times are meant for liquids only. Heat is transfered through solids by conduction so if you have little undissolved chunks of something the center of the chunks are going take a little longer to heat up than the liquid they're in.
--------------------
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: pour agar TEK [Re: taGyo]
#22222187 - 09/11/15 02:08 PM (8 years, 4 months ago) |
|
|
|
|