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Cthulhu23
Elder God Extraordinaire



Registered: 07/04/18
Posts: 620
Last seen: 2 years, 6 months
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Re: BOD's Comprehensive Agar TEK [Re: chaosshadow]
#25550180 - 10/19/18 12:26 PM (5 years, 3 months ago) |
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Quote:
chaosshadow said: Hey Bodhi 2 questions 
Switching from PDA to Oat Water for my Agar nute medium.
1. Will there be a noticeable difference between the growing mediums besides the coloring? anything I should know?
2. How much should I dilute the Oat Water? 50/50 Water/OatWater?
Thanks!
I can’t speak for PDA, but I noticed a little difference going from BRF agar to GWA. Lighter growth to start that fills in thicker over time. But I don’t notice it making much of a difference.
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chaosshadow
FungiFondler


Registered: 02/26/17
Posts: 188
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Re: BOD's Comprehensive Agar TEK [Re: Cthulhu23]
#25551076 - 10/19/18 06:51 PM (5 years, 3 months ago) |
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Alright! thanks for your input, you dont dilute either I suppose.
EDit:
Hey bodhi, how many grams of agar agar would you add to 400 ml of grain water? Thanks!
Edited by chaosshadow (10/19/18 07:04 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: BOD's Comprehensive Agar TEK [Re: chaosshadow] 1
#25551132 - 10/19/18 07:40 PM (5 years, 3 months ago) |
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i use 2% agar and it works wonderfully. that's 20 grams per liter. that'd be 8 grams to 400
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Worked good for me 
Here’s some plates T1 ksss clone, and Ecuador ms plate in the middle. Did 8g brf 8g agar for 400ml.

Going to take another transfer.
It’s cool seeing it grow! Reminds me of each one being a snowflake, unique in their own ways.
Thank you bod this is fun!
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chaosshadow
FungiFondler


Registered: 02/26/17
Posts: 188
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alright I will try that ! thanks!!!
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C45
Stranger

Registered: 01/23/17
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Re: BOD's Comprehensive Agar TEK [Re: chaosshadow]
#25576594 - 10/29/18 02:49 PM (5 years, 3 months ago) |
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Really helpful thanks
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Ep1429
Stranger
Registered: 10/10/18
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Re: BOD's Comprehensive Agar TEK [Re: C45]
#25593161 - 11/05/18 05:42 AM (5 years, 2 months ago) |
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Maybe someone has asked this, I haven't taken the time to read but the first ten pages of this thread, but suppose you're a guy just looking to do 6-8 jars every now and then for you and friends. If I understand this correctly, I should be able to get by with 10 Pyrex Petri dishes, correct?
One Petri dish gets a drop from my MS syringe, smeared with an inoculation loop or a sterile q-tip (I have access to vet supplies), when the dish colonizes, I use an exacto knife to remove a rice sized piece of myc, and put it into a second petri. Let it colonize, and if clean, use a wedge per jar on two jars, case them in a shoebox, pick the best performing mushie and clone it on the third or fourth dish.
The third (or fourth depending on how long it takes to get clean myc) dish becomes the new master, and I clean it up with another transfer or two. That's five or six Petri dishes. That cleaned clone master is then used to colonize the remaining dishes, and is itself stored in a fridge for future use.
I have used three syringes of the four I purchased, and they have had about a 50% success rate. My sterile technique is perfect, I'm no stranger to scientific research in a lab, so I know the contams are coming via the MS syringe and it's driving my OCD absolutely bat shit crazy. Thanks to work, I can get a sleeve of ten pre-poured Pyrex reusable dishes by the end of the week for $20, and it's looking like I should go this route for my last syringe.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: BOD's Comprehensive Agar TEK [Re: Ep1429]
#25593204 - 11/05/18 06:25 AM (5 years, 2 months ago) |
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Quote:
Let it colonize, and if clean, use a wedge per jar on two jars, case them in a shoebox, pick the best performing mushie and clone it on the third or fourth dish.
No idea what this or "do 6-8 jars every now and then" means. Are you trying to case and fruit an agar plate? If you're curious if 10 plates is enough to grow a small, personal amount, then yes, especially with your perfect sterile technique. But not by fruiting on plates or something.
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Ep1429
Stranger
Registered: 10/10/18
Posts: 800
Last seen: 2 years, 10 months
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Quote:
stareatclouds said:
Quote:
Let it colonize, and if clean, use a wedge per jar on two jars, case them in a shoebox, pick the best performing mushie and clone it on the third or fourth dish.
No idea what this or "do 6-8 jars every now and then" means. Are you trying to case and fruit an agar plate? If you're curious if 10 plates is enough to grow a small, personal amount, then yes, especially with your perfect sterile technique. But not by fruiting on plates or something.
6-8 “jars” would seem to indicate that I wasn’t planning on putting the colonized agar wedges into another “plate” or “dish”, don’t you think? 
Just looking to do two or three shoeboxes once or twice a year. Mushies make great Christmas and birthday presents. Not sure yet how big a wedge would be required from a 100mm Petri dish to colonize a quart jar of wbs with some semblance of speed. If I can get 3-4 jars per Petri dish, I’d think 10 pre-poured Petri dishes could last a year or two.
Edit: And yes, my technique is perfect. I’ve been working with blood in a science lab for 12 years, I’m not prone to errors.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: BOD's Comprehensive Agar TEK [Re: Ep1429]
#25593656 - 11/05/18 10:47 AM (5 years, 2 months ago) |
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a dish does 4 jars without a problem. i've done more but usually don't go over four. bulk grows will give you more than enough for christmas and birthday presents, unless you have a lot of friends that really like the magic. agar plates last quite a while but will dry out and go bad eventually. if you want to pre pour and save long term i'd suggest wrapping well, putting into ziplock bags, and into the fridge. but i wouldn't really recommend that. it's easy enough to have a pouring session once every couple of months.
i just checked in on my current, "how long will these plates last" test. on 5/1/18 i poured a bunch of plates. i save one thin poured plate and one thick. they are both looking totally fine. so that's six months. they are just sitting on the table in my grow space. semi stable temp 70-72 F.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: BOD's Comprehensive Agar TEK [Re: Ep1429]
#25593869 - 11/05/18 12:14 PM (5 years, 2 months ago) |
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Quote:
Ep1429 said: 6-8 “jars” would seem to indicate that I wasn’t planning on putting the colonized agar wedges into another “plate” or “dish”, don’t you think? 
I think you didn't mention grain jars at all so I had no clue what you meant.
Quote:
Not sure yet how big a wedge would be required from a 100mm Petri dish to colonize a quart jar of wbs with some semblance of speed. If I can get 3-4 jars per Petri dish, I’d think 10 pre-poured Petri dishes could last a year or two.
This is what you should've asked. Yes, you can split a dish into 4 quart jars, no problem. The speed depends on the culture, when you shake it, moisture content of jars, etc.
I think you should just buy some plates and pour agar whenever you want to make more and not try and stretch 10 plates for so long.
verum,
You should make a whole stack of them and try and use one every month or something. In my experience, you lose a lot of the viability with moisture loss, which isn't always easy to spot. And the plates won't contam while this is happening.
It's likely because they're old and less secure, but my older PP's will look totally fine (beyond cracking from drying out) for 3+ months. And then I'll take a transfer and within 2 days, there's 100 satellites in there, which I highly doubt I put in with the transfer. Doesn't happen with dishes, though, so I'm sure it's utensil error.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Last seen: 1 year, 7 months
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interesting. I'll try these plates out now that they are 6 months old. i did wrap them a lot to try and prolong shelf life by preventing moisture loss. three or four wraps of glad, if i remember right. i usually just wrap plates twice with glad.
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Ep1429
Stranger
Registered: 10/10/18
Posts: 800
Last seen: 2 years, 10 months
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Quote:
stareatclouds said:
Quote:
Ep1429 said: 6-8 “jars” would seem to indicate that I wasn’t planning on putting the colonized agar wedges into another “plate” or “dish”, don’t you think? 
I think you didn't mention grain jars at all so I had no clue what you meant.
Pretty sure that’s how I ended the second paragraph. Colonized wedges into jars and what not.
I will begin pouring agar, but if I can get pre-poured plates for roughly the same price as blanks, why not save myself the labor?
Speaking of...
Is it still SOP to use agar-agar and grain soak water? We get a malt extract agar mix in our Petri dishes, antibiotics already added. Those should still work, correct?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: BOD's Comprehensive Agar TEK [Re: Ep1429]
#25594198 - 11/05/18 03:43 PM (5 years, 2 months ago) |
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People use jars for tons of shit. It's best to specify to avoid ambiguity.
Sure, if literally all you are willing to use are expensive glass dishes that are a PITA to handle, buy those. No idea how your math is working out, but I got 1000 sterile dishes for like $80 and it probably costs me something like $.20 for a stack of 20. I know I'd prefer to make my own than drop $20 on 10 glass plates, especially with what I assume is access to lab equipment, but to each their own.
There's a million recipes. I'd say SOP around here is just malt and agar. Your mix should be fine.
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Ep1429
Stranger
Registered: 10/10/18
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Last seen: 2 years, 10 months
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I'd prefer not to have to explain to my boss why I ordered a thousand extra Petri dishes and why they aren't in the inventory.
In the same vein as I'd prefer not to have my mailman know I had them shipped to my house and not the office.
You probably use your credit or debit card for purchases and not a pre-paid gift card that isn't attached to your name, huh? Playing with fire, IMO.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: BOD's Comprehensive Agar TEK [Re: Ep1429]
#25595502 - 11/06/18 07:50 AM (5 years, 2 months ago) |
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You dont need to buy 1000's I just ordered 40 off Amazon for under a fiver (12.5p each) and the mail man knows nothing about it
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: BOD's Comprehensive Agar TEK [Re: Ep1429] 1
#25595563 - 11/06/18 08:27 AM (5 years, 2 months ago) |
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Quote:
Ep1429 said: I'd prefer not to have to explain to my boss why I ordered a thousand extra Petri dishes and why they aren't in the inventory.
In the same vein as I'd prefer not to have my mailman know I had them shipped to my house and not the office.
You probably use your credit or debit card for purchases and not a pre-paid gift card that isn't attached to your name, huh? Playing with fire, IMO.
Lol wut. Ordering petri dishes is playing with plastic.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
#25595598 - 11/06/18 08:44 AM (5 years, 2 months ago) |
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This dude has been working in a lab practicing 100% perfect technique since 1847, but thinks it's suspicious buying a sleeve of plates online? Anyway, yeah bro, good luck with your dishes and jars.
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Got some plates that have been fully colonized to the sides for a couple days now. How long can they go before being used for grains? I plan on using them tomorrow.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: BOD's Comprehensive Agar TEK [Re: MadHatter333]
#25596584 - 11/06/18 03:55 PM (5 years, 2 months ago) |
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You want to use them as soon as possible. When looking for long term storage most people put plates in the fridge immediately after inoculation. It slows the mycelium. It varies but your plates should be fine for weeks.
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