Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: < First | < Back | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleGerms
Space Force
 User Gallery

Registered: 06/26/11
Posts: 4,607
Loc: Texas
Trusted Cultivator
Re: pour agar TEK [Re: chaosshadow]
    #25534492 - 10/13/18 12:49 PM (5 years, 3 months ago)

Quote:

chaosshadow said:
Hey man thanks for your answer!
should i wrap them with the wrap? I use bods wrap tek
or should i just put them all in a zip bag.



I use glad cling wrap, you can put them inside a ziploc bag as an extra precaution if you’d like, although I don’t think it is necessary


--------------------


Extras: Filter Print Post Top
Offlinetathlyn
Perpetual Noob
Male User Gallery


Registered: 08/31/18
Posts: 151
Loc: Persia
Last seen: 3 months, 26 days
Re: pour agar TEK [Re: Germs] * 1
    #25537168 - 10/14/18 03:24 PM (5 years, 3 months ago)

@Bodhisatta

I purchased a similar bottle that is a little thinner and labeled as easy pour. They also run a buck cheaper. For us old folks this is easy to maneuver and pours cleanly.




https://www.amazon.com/gp/offer-listing/B00QU9BVH6


--------------------
Beware of unearned wisdom.
  - Carl Jung


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: pour agar TEK [Re: tathlyn] * 1
    #25537179 - 10/14/18 03:26 PM (5 years, 3 months ago)


:rockon:


Extras: Filter Print Post Top
OfflineHuckleBerrySlim
Male User Gallery

Registered: 10/07/18
Posts: 239
Last seen: 5 years, 1 month
Re: pour agar TEK [Re: bodhisatta]
    #25537994 - 10/14/18 09:28 PM (5 years, 3 months ago)

Where do you order your 500ml media bottles? I’ve seen these with the blue caps and rings before but only on amazon and only 250ml I can’t find the 500ml anywhere.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: pour agar TEK [Re: HuckleBerrySlim]
    #25538058 - 10/14/18 09:59 PM (5 years, 3 months ago)

Amazon


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: pour agar TEK [Re: bodhisatta]
    #25538106 - 10/14/18 10:18 PM (5 years, 3 months ago)

i still like my captain morgan handle


Extras: Filter Print Post Top
Invisiblechaosshadow
FungiFondler
I'm a teapot User Gallery

Registered: 02/26/17
Posts: 188
Re: pour agar TEK [Re: Germs]
    #25540221 - 10/15/18 07:04 PM (5 years, 3 months ago)

Quote:

Germs said:
Quote:

chaosshadow said:
Hey man thanks for your answer!
should i wrap them with the wrap? I use bods wrap tek
or should i just put them all in a zip bag.



I use glad cling wrap, you can put them inside a ziploc bag as an extra precaution if you’d like, although I don’t think it is necessary



Thanks! one more question, how long do spores take to "germinate"(?) in agar? This is the first time doing spore syringe to agar, I have always started with LC and then move to agar, the process is a bit faster IG.


How long does it take to spores to do something on agar? on average?


--------------------


Extras: Filter Print Post Top
Offlinegizmodo
Woodland Creature
Male


Folding@home Statistics
Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
Re: pour agar TEK [Re: chaosshadow]
    #25540271 - 10/15/18 07:21 PM (5 years, 3 months ago)

Quote:

chaosshadow said:
Quote:

Germs said:
Quote:

chaosshadow said:
Hey man thanks for your answer!
should i wrap them with the wrap? I use bods wrap tek
or should i just put them all in a zip bag.



I use glad cling wrap, you can put them inside a ziploc bag as an extra precaution if you’d like, although I don’t think it is necessary



Thanks! one more question, how long do spores take to "germinate"(?) in agar? This is the first time doing spore syringe to agar, I have always started with LC and then move to agar, the process is a bit faster IG.


How long does it take to spores to do something on agar? on average?




A 24 hours to a week or more.


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: pour agar TEK [Re: gizmodo]
    #25544882 - 10/17/18 02:31 PM (5 years, 3 months ago)

yeah, it all depends how healthy and/or young the spores are. fresh healthy spores can show germination in a day or two. i've also had spores chill for a month and then germ so... somewhere between a day and a month.


Extras: Filter Print Post Top
Invisiblemushfuss
Bubbles
 User Gallery


Registered: 06/20/18
Posts: 126
Re: BOD's Comprehensive Agar TEK [Re: Severance13]
    #25544916 - 10/17/18 02:44 PM (5 years, 3 months ago)

Quote:

Severance13 said:
Hey everyone, do you have a recommendation on a microwave that's affordable and fits the bottle? Mine's too small to hold a 500ml agar bottle, and going place to place through craigslist and other places seems difficult. It'd be easier to sort by make and model through those resources


  :nyan:


Your attention to specificity is admirable. Relying on others for a microwave choice on the other hand..
:hug:

If you can tilt it enough and watch it well enough for the flash-boil, you'll be fine. It gets messy quick once you get bubbles.


Extras: Filter Print Post Top
Invisibledmar
I'm a teapot

Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: pour agar TEK [Re: bodhisatta]
    #25545911 - 10/17/18 10:44 PM (5 years, 3 months ago)

Sorry if this is a stupid question, but does it matter if your spores are dry or hydrated when you use the inoculation loop to put them on agar? I just did it for the first time like 5 minutes ago and what I did was sterilize water, put a few drops on my dry spore print, then swab the inoculation loop on it, then the zig zag on the agar. Reason I ask is because I noticed in your video, it looked like you were doing it with dry spores, but here you talked about putting them in the shot glass if from a syringe. So it shouldn't matter wet or dry right?

Also I was about to do another one with live tissue. I watched the let's grow mushrooms videos, and he tears the cap open, cuts up tissue, then sets it on top of the agar. Do I have that right?


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: pour agar TEK [Re: dmar]
    #25546012 - 10/17/18 11:59 PM (5 years, 3 months ago)

If spores are old/dry, germination can be tougher, so more moisture may be needed. But I think making "softer" agar (less agar per volume of water) is better than getting your spore print wet.

For tissue samples, you tear open a fruit (as the inside hasn't been exposed to the outer air) and scrape a piece of tissue out and place it on agar, yes.


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: pour agar TEK [Re: stareatclouds]
    #25547487 - 10/18/18 01:36 PM (5 years, 3 months ago)

i would not recommend putting drips of water on your print. prints are not perfectly clean and water running around will just act as a contam superhighway  (in my opinion). also, hydrating spores is not just a matter of getting them wet for a second. they need to "drink" the water and get it in them. this can take a little time. for this reason we either put old/dry spores on soft agar, like stare said, or scrape some off of the print into sterile water to let them hydrate for a while before putting to agar.


Extras: Filter Print Post Top
InvisibleOrganic Chemist
Female User Gallery

Registered: 04/10/17
Posts: 126
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25549706 - 10/19/18 09:39 AM (5 years, 3 months ago)

What recipe do you use for your brown rice flour agar?  flour(g), agar(g), city water (ml)?  Does the brand agar change things or are they fairly consistent?  Thanks.


Extras: Filter Print Post Top
InvisibleOrganic Chemist
Female User Gallery

Registered: 04/10/17
Posts: 126
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25549737 - 10/19/18 09:49 AM (5 years, 3 months ago)

Okay, I just found it in another thread.  Is this still correct?

6g brown rice flower
9g agar
400ml city water


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: Organic Chemist]
    #25549744 - 10/19/18 09:53 AM (5 years, 3 months ago)

Yea anything around 1-2% works pretty good


Extras: Filter Print Post Top
OfflineCthulhu23
Elder God Extraordinaire
Male


Registered: 07/04/18
Posts: 620
Last seen: 2 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: Organic Chemist]
    #25550158 - 10/19/18 12:16 PM (5 years, 3 months ago)

Quote:

Organic Chemist said:
Okay, I just found it in another thread.  Is this still correct?

6g brown rice flower
9g agar
400ml city water




I’ve been using this exact recipe successfully for months.  I prefer GWA simply bc it’s clearer and easier to see contams, but I don’t always have the grain water on hand.


Extras: Filter Print Post Top
Invisiblechaosshadow
FungiFondler
I'm a teapot User Gallery

Registered: 02/26/17
Posts: 188
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25550167 - 10/19/18 12:19 PM (5 years, 3 months ago)

Hey Bodhi 2 questions :laugh:

Switching from PDA to Oat Water for my Agar nute medium.

1. Will there be a noticeable difference between the growing mediums besides the coloring? anything I should know?

2. How much should I dilute the Oat Water? 50/50 Water/OatWater?


Thanks!


--------------------


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: BOD's Comprehensive Agar TEK [Re: chaosshadow]
    #25550172 - 10/19/18 12:20 PM (5 years, 3 months ago)

I don't dilute the grain water at all.


Extras: Filter Print Post Top
Invisiblechaosshadow
FungiFondler
I'm a teapot User Gallery

Registered: 02/26/17
Posts: 188
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25550176 - 10/19/18 12:22 PM (5 years, 3 months ago)

Oh I read it somewhere so I thought it was a thing, specially because the oat water looks kinda dark,but Ill try it :laugh:


--------------------


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | Next > | Last >

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Agar Tek Aristar 5,815 12 08/01/03 06:42 PM
by shirley knott
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,732 8 12/13/19 06:16 PM
by mushboy
* Re: Peroxidated Agar Tek sparks 4,555 6 05/08/00 12:43 AM
by Anonymous
* agar tek? the man 2,773 4 12/16/02 10:45 AM
by the man
* Agar ;)
( 1 2 all )
SCIOpenEyedDream 7,182 25 09/01/04 09:58 AM
by fastfred
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,285 38 05/11/01 11:08 AM
by MAIA
* Mycelium-only tek, Please Help! FaminedShaman 2,563 4 05/06/01 06:49 AM
by egghead
* Antibac Agar, DIY! superduper 4,846 13 12/04/02 02:20 PM
by superduper

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
640,984 topic views. 36 members, 236 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.01 seconds on 15 queries.