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OfflineCthulhu23
Elder God Extraordinaire
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Registered: 07/04/18
Posts: 620
Last seen: 2 years, 6 months
Re: pour agar TEK [Re: verum subsequentis]
    #25436317 - 09/04/18 09:47 PM (5 years, 4 months ago)

Quote:

verum subsequentis said:
if you are new to agar and have a couple plates looking just like that then you a rockin the fuck out man. :superthrust: good job. you can always drop most of it to grain and keep a little on a couple of plates.




Thanks, Verum!  I am new to agar.  This was one of the first transfers I ever did.  Good to know I’m doing it right.


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OfflineDayglo Joe

Registered: 08/15/18
Posts: 101
Last seen: 2 years, 3 months
Re: pour agar TEK [Re: Cthulhu23] * 1
    #25443347 - 09/07/18 06:51 PM (5 years, 4 months ago)

Amazing amount of info!
So.... comprehensive!
Ill be using this very soon!
Thank you!

:takingnotes:


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Offlinegizmodo
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Registered: 06/21/18
Posts: 2,064
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Re: pour agar TEK [Re: Dayglo Joe]
    #25443427 - 09/07/18 07:23 PM (5 years, 4 months ago)

Quote:

Dayglo Joe said:
Amazing amount of info!
So.... comprehensive!
Ill be using this very soon!
Thank you!

:takingnotes:




Welcome to the Shroomery.


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


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InvisibleBumholio
What's the craic
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Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: pour agar TEK [Re: gizmodo]
    #25444213 - 09/08/18 01:28 AM (5 years, 4 months ago)

I'm struggling with getting my agar right atm. I have a few plates i knocked on the 21st and they have started to colonize about a week ago but they are really really slow


--------------------


"great things may come to those who wait, but only what's left by those who hustle"


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InvisiblePhishDicks
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Registered: 07/13/17
Posts: 12
Re: pour agar TEK [Re: bodhisatta]
    #25471716 - 09/19/18 12:40 AM (5 years, 4 months ago)

Newb question here. I am having good success with my first spore to agar inoculation and subsequent transfers.  I am meticulously wrapping each dish with parafilm.  Does that stifle the necessary gas exchange for optimal colonization of the plate?







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InvisibleBumholio
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Registered: 07/23/18
Posts: 4,269
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Re: pour agar TEK [Re: PhishDicks]
    #25471771 - 09/19/18 01:15 AM (5 years, 4 months ago)

No


--------------------


"great things may come to those who wait, but only what's left by those who hustle"


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: pour agar TEK [Re: Bumholio]
    #25471802 - 09/19/18 02:04 AM (5 years, 4 months ago)

depends on what you mean by "meticulously wrapping". each wrap restricts more air. how much are you wrapping. one wrap is fine but some do two to be "safe". i wrap twice with glad cling wrap


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InvisiblePhishDicks
Stranger

Registered: 07/13/17
Posts: 12
Re: pour agar TEK [Re: verum subsequentis]
    #25471958 - 09/19/18 05:20 AM (5 years, 4 months ago)

Thanks! Only one wrap here. It has been doing well, so Ill stick with it.  Just wanted some more experience than mine to weigh in.


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Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: pour agar TEK [Re: PhishDicks]
    #25472217 - 09/19/18 08:08 AM (5 years, 4 months ago)

Agar plates don't need gas exchange. I do 2. That's how bod does it in his dish video.


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InvisibleSilentShrooms
Who Am I?
I'm a teapot
Registered: 09/07/18
Posts: 10
Re: pour agar TEK [Re: boom boom]
    #25475703 - 09/20/18 03:38 PM (5 years, 4 months ago)

I've used this TEK for some time now, great results, and is easy AF...thanks again bod :thumbup:


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Offline5DGISD
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Registered: 04/30/18
Posts: 26
Last seen: 3 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25480846 - 09/22/18 01:28 PM (5 years, 4 months ago)

hey BOD....thanks for the tek, I'm going to give agar a try for the first time. I have all I need as far as materials but I have a question....what about the petri dishes....I have a pack of glass dishes but I don't see anyone explaining whether or not you need to sterilize the dishes first before you pour.....my heart and mind says yes but.......if so, what is a good procedure for this. Can it be done the same time you sterilize your agar.


--------------------
Psychedelics can produce psychotic behaviour in the people who haven't taken them.


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: BOD's Comprehensive Agar TEK [Re: 5DGISD]
    #25480866 - 09/22/18 01:37 PM (5 years, 4 months ago)

i'm not bod but i know the answers. yes they need sterilized unless they are new, came sterile and are unopened. yes they can be sterilized along with you agar but you must be sure that they remain sterile. they can be wrapped in foil, sterilized and then unwrapped once inside the SAB or in the flow.


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Offline5DGISD
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Registered: 04/30/18
Posts: 26
Last seen: 3 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #25480881 - 09/22/18 01:44 PM (5 years, 4 months ago)

ahh, yes thank you. Never considered wrapping them up.......this tek calls for 20 min @ 15lbs to sterilize the agar.....that's going to be enough for the dishes as well?


--------------------
Psychedelics can produce psychotic behaviour in the people who haven't taken them.


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
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Re: BOD's Comprehensive Agar TEK [Re: 5DGISD]
    #25480908 - 09/22/18 02:02 PM (5 years, 4 months ago)

yep. more than enough. you don't have to wrap them but you do have to do something to ensure that they don't become dirty after sterilizing them. contams are everywhere and wrapping them nicely in foil is a really effective and easy way to protect them.


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Offline5DGISD
Stranger
Registered: 04/30/18
Posts: 26
Last seen: 3 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #25480935 - 09/22/18 02:13 PM (5 years, 4 months ago)

I am going to be using my pressure cooker after sterilization to do the cool down procedure, so wrapping them up will keep them clean until everything goes in the SAB.....thanks for the heads up......ok, I'm going in......


--------------------
Psychedelics can produce psychotic behaviour in the people who haven't taken them.


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: 5DGISD]
    #25481173 - 09/22/18 03:55 PM (5 years, 4 months ago)

Quote:

5DGISD said:
hey BOD....thanks for the tek, I'm going to give agar a try for the first time. I have all I need as far as materials but I have a question....what about the petri dishes....I have a pack of glass dishes but I don't see anyone explaining whether or not you need to sterilize the dishes first before you pour.....my heart and mind says yes but.......if so, what is a good procedure for this. Can it be done the same time you sterilize your agar.



Under glass dish sterilization on the first post in this thread. But yes they need to be sterile or they'll contaminate your agar


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Offline5DGISD
Stranger
Registered: 04/30/18
Posts: 26
Last seen: 3 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25481274 - 09/22/18 04:52 PM (5 years, 4 months ago)

:-)....yeah, I've read so many agar teks in the last couple days it gets confusing....but thanks for posting up your know how


--------------------
Psychedelics can produce psychotic behaviour in the people who haven't taken them.


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Invisibledmar
I'm a teapot


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25483545 - 09/23/18 05:21 PM (5 years, 4 months ago)

Good read. I'm doing a SGFC grow, and a shoebox grow as soon as my PF TEK jars finish colonizing, and i'd like to learn this so I can clone for the next one. So much to learn lol


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OfflineCatfishlily
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Registered: 09/10/18
Posts: 14
Last seen: 3 years, 6 months
Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25484407 - 09/24/18 04:01 AM (5 years, 4 months ago)

Cool


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Offlineanahanini
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Registered: 01/03/18
Posts: 52
Last seen: 3 years, 4 months
Re: BOD's Comprehensive Agar TEK [Re: Catfishlily]
    #25484566 - 09/24/18 07:32 AM (5 years, 4 months ago)

thanks.


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