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Offlineverum subsequentis
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Re: pour agar TEK [Re: Cthulhu23]
    #25394549 - 08/17/18 01:38 PM (5 years, 5 months ago)

they might be and they might not be. pics would help but yes, there can be hidden (if you don't know what to look for) contams on a fully colonized plate.


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OfflineCthulhu23
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Re: pour agar TEK [Re: verum subsequentis]
    #25394901 - 08/17/18 05:07 PM (5 years, 5 months ago)

Sure, here is one, for example



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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: Cthulhu23]
    #25397316 - 08/18/18 09:13 PM (5 years, 5 months ago)



Pic 5 the RW clone tissue rolled around trying to get off scalpel so i think thats the weird spot


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Offlinedrprobablyno
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25404864 - 08/22/18 09:41 AM (5 years, 5 months ago)

Amazing. Thanks Bod. Very useful.


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Offlinegizmodo
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Re: pour agar TEK [Re: drprobablyno]
    #25432882 - 09/03/18 12:14 PM (5 years, 4 months ago)

Amazing write up my friend. Thank you for sharing the information in a concise and easily digestible format.


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One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
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Say No To Grow Kits


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InvisibleMadHatter333
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Re: pour agar TEK [Re: gizmodo]
    #25432912 - 09/03/18 12:31 PM (5 years, 4 months ago)

Is it okay to run just one 500ml bottle in the pc or do you need to have it more full then that? Going to start my first plates soon.


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Offlinegizmodo
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Re: pour agar TEK [Re: MadHatter333]
    #25432930 - 09/03/18 12:40 PM (5 years, 4 months ago)

Quote:

MadHatter333 said:
Is it okay to run just one 500ml bottle in the pc or do you need to have it more full then that? Going to start my first plates soon.




I think so long as the bottle has something keeping it from falling over or something while in the PC it will be fine, that way water doesn't get into it if that is even possible with the container you use.
I've yet to do any of this yet but from what I've read that seems like an answer that might help you someone could clarify though hopefully.


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


Edited by gizmodo (09/03/18 12:40 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: gizmodo] * 1
    #25432950 - 09/03/18 12:48 PM (5 years, 4 months ago)

One at a time is fine


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InvisibleMadHatter333
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Re: pour agar TEK [Re: bodhisatta] * 1
    #25433172 - 09/03/18 02:19 PM (5 years, 4 months ago)

Cool, I’ll just run one than. Thanks guys.


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Offlinebakenast
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Re: pour agar TEK [Re: Cthulhu23]
    #25433305 - 09/03/18 03:50 PM (5 years, 4 months ago)

Quote:

Cthulhu23 said:
Sure, here is one, for example





Is his a clean plate then? I have a few very similar to this I’m wanting to drop to grain,, but before they hit the edge,,


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Offlinegizmodo
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Re: pour agar TEK [Re: bakenast]
    #25433316 - 09/03/18 03:53 PM (5 years, 4 months ago)

Quote:

bakenast said:
Is his a clean plate then? I have a few very similar to this I’m wanting to drop to grain,, but before they hit the edge,,




Sorry but are you asking if that plate in the post image from Cthulhu is a clean plate example?


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One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


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Offlinebakenast
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Re: pour agar TEK [Re: gizmodo]
    #25433325 - 09/03/18 03:58 PM (5 years, 4 months ago)

Yea the one I quoted under..  mine look identical to his but at 90% I don’t want to do my 3rd Transfer if looking like that is good enough to drop in jar .. thanks


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Offlinegizmodo
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Re: pour agar TEK [Re: bakenast]
    #25433351 - 09/03/18 04:07 PM (5 years, 4 months ago)

I think that was showing a plate with hidden contaminates but I'm not sure.
Sent him a message to point him this way for you.
Since he hasn't had a chance to drop you a line refer to the link below.

Contaminations


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


Edited by gizmodo (09/04/18 10:40 AM)


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OfflineMustmyco
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25434987 - 09/04/18 12:26 PM (5 years, 4 months ago)

First off thank you for the write up second if it's 20g/L brf
That makes it 10g/ per bottle right?

And if there is sediment issues could you just put a little less or does it not really affect the agar ?


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New to all of this (: if you have any advice let me know


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Offlineverum subsequentis
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Re: pour agar TEK [Re: bakenast]
    #25435074 - 09/04/18 01:05 PM (5 years, 4 months ago)

Quote:

bakenast said:
Quote:

Cthulhu23 said:
Sure, here is one, for example





Is his a clean plate then? I have a few very similar to this I’m wanting to drop to grain,, but before they hit the edge,,




it's hard to say for sure if that plate is clean or has some contams hiding. i'd say it's clean but some would say that it looks suspicious. if you have plates that look like that you are probably good to go but to be sure, post a picture and let us have a look.


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Re: pour agar TEK [Re: verum subsequentis]
    #25435293 - 09/04/18 03:34 PM (5 years, 4 months ago)

What part of the plat looks suspicious? Or is it the entire thing?  I was thinking it looked clean lol


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Offlineverum subsequentis
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Re: pour agar TEK [Re: bakenast]
    #25435434 - 09/04/18 04:33 PM (5 years, 4 months ago)

it's not my opinion but i know some that would say that the fuzzy undefined part surrounding the transfer could be indicative of some bacteria and some would also say that cultures that go extremely rhizo like that could also be running from bacteria.

again, i don't really think that but some people around here would. i'd say it's probably clean but i would take another transfer and watch it closely just to be sure.


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Re: pour agar TEK [Re: verum subsequentis]
    #25435499 - 09/04/18 04:59 PM (5 years, 4 months ago)

Oh I wasn’t arguing, I just have a couple identical looking to his, and I’m newish to agar.. and was hoping mine was clean to, was gonna drop some chunks to grain and try making a slant.. but maybe I rushing it


Edited by bakenast (09/04/18 05:27 PM)


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Offlineverum subsequentis
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Re: pour agar TEK [Re: bakenast]
    #25436052 - 09/04/18 08:14 PM (5 years, 4 months ago)

if you are new to agar and have a couple plates looking just like that then you a rockin the fuck out man. :superthrust: good job. you can always drop most of it to grain and keep a little on a couple of plates.


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Re: pour agar TEK [Re: verum subsequentis]
    #25436113 - 09/04/18 08:34 PM (5 years, 4 months ago)

Not really lol , I usually just multispore to grain works good in winter months but in summer trich issues are horrible. Only reason I’m doing agar is to get something clean..  lol everyone hates MS to grain but in winter months I have very good success not perfect but good..      I actually have a black mold in a spawn bag I’m gonna up load a pic I have never seen in my life looks like coffee ground patches ,, but never put an coffee grounds in with my grains. Lol


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