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OfflineSoulsearchin2018
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Re: pour agar TEK [Re: Legend21]
    #25307965 - 07/04/18 10:17 AM (5 years, 6 months ago)

Thanks! Yes they are from a spore syringe. I am waiting for my mini tupper ware pasty plates to dry out a little before attempting my first transfer later today.


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OfflineLegend21
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Re: pour agar TEK [Re: Soulsearchin2018]
    #25308020 - 07/04/18 11:03 AM (5 years, 6 months ago)

Yeah as soon as you have time just take a small piece from each plate and let it grow for a few days on the next plate to see how it looks


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OfflineNorthernKronic
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Re: pour agar TEK [Re: Legend21]
    #25308191 - 07/04/18 12:30 PM (5 years, 6 months ago)

Thanks so much for this TEK Bod. Here is a pic of a couple plates that I transferred on 6-25 off of my MS Plates. All are PE..





Cut these up and put them to grain jars today in the SAB. Stoked to see what they look like in a week!!


Thanks again BOD!!

-N.Kron


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Offlinekerberus
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Re: BOD's Comprehensive Agar TEK [Re: kerberus]
    #25309169 - 07/05/18 02:36 AM (5 years, 6 months ago)

Quote:

kerberus said:
Cheers verum,
just about to transfer now and will not re-use plates!

I will do this plate first and see if i can get a better shot of the other one.

Thank you for the reply.

Edit. 3 hours later and transferring to new plates is not as easy as i was expecting! pretty certain 2 of the 6 plates have no transferred material and look like Freddy Kruger has had his hand in it.
On a positive note poured these in the hope that i haven't totally f-up the original plate and the 6 new ones.





OK, managed a better photo of the other plate does this look like bacteria as i am concerned about the wispy star shaped bits! Cheers guys.


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InvisibleMadHatter333
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25310837 - 07/05/18 10:34 PM (5 years, 6 months ago)

When you say 20g/L is that twenty grams to a liter of water? So it would be 10g for a 500ml media bottle? Just making sure I read it right. Also got myself a microwave and am going to get a mini fridge for storage, media bottle, and some dishes. I want to get serious with agar after trying pasty plates and having some success.


--------------------

TEKs I Like


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Offlinefraufranfroufrou
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Re: BOD's Comprehensive Agar TEK [Re: MadHatter333]
    #25311027 - 07/06/18 03:33 AM (5 years, 6 months ago)

Quote:

MadHatter333 said:
When you say 20g/L is that twenty grams to a liter of water? So it would be 10g for a 500ml media bottle?




1g per 50ml. Bod only fills the 500ml bottle to 400ml so that's 8g.


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InvisibleMadHatter333
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Re: BOD's Comprehensive Agar TEK [Re: fraufranfroufrou]
    #25312116 - 07/06/18 05:28 PM (5 years, 6 months ago)

Quote:

fraufranfroufrou said:
Quote:

MadHatter333 said:
When you say 20g/L is that twenty grams to a liter of water? So it would be 10g for a 500ml media bottle?




1g per 50ml. Bod only fills the 500ml bottle to 400ml so that's 8g.




Got it! Thanks (:


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TEKs I Like


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InvisibleNakoa

Registered: 03/27/18
Posts: 170
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Re: BOD's Comprehensive Agar TEK [Re: MadHatter333]
    #25330157 - 07/16/18 04:16 PM (5 years, 6 months ago)

First time trying this Tek !

This dish is three transfers from a clone.



This dish is from a pin that grew out on the original clone dish.



✌️


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OfflineSoulsearchin2018
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Re: BOD's Comprehensive Agar TEK [Re: Nakoa]
    #25331090 - 07/17/18 07:58 AM (5 years, 6 months ago)

My agar iskills are definitely improving

1st pour:


Next 3 generations of pours, tried pasty plates, didn’t like them, moved back to peteris, changed recipes and added food coloring...



I love this shit!

Thanks to all the contributors for the help and teks


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: Soulsearchin2018]
    #25337446 - 07/20/18 05:26 PM (5 years, 6 months ago)

Just got myself a "Pyrex Slimline Media Bottle Easy Pour Corning 500ML Glass"

looks cool. well made. was $17.90 so a bit pricey for a bottle.


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InvisibleGerms
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25337470 - 07/20/18 05:43 PM (5 years, 6 months ago)

Quote:

bodhisatta said:
Just got myself a "Pyrex Slimline Media Bottle Easy Pour Corning 500ML Glass"

looks cool. well made. was $17.90 so a bit pricey for a bottle.



I’ve seen those on amazon. Looks like it would be even easier to hold than the standard. Might have to cop me one


--------------------


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OfflineSoulsearchin2018
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Re: BOD's Comprehensive Agar TEK [Re: Germs]
    #25338203 - 07/21/18 12:31 AM (5 years, 6 months ago)

These are my first set of agar inoculated grain jars. Are they ready for a shake?



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Offlineverum subsequentis
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Re: BOD's Comprehensive Agar TEK [Re: Soulsearchin2018]
    #25338208 - 07/21/18 12:33 AM (5 years, 6 months ago)

absolutely.


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OfflineELEVATI0N
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Re: BOD's Comprehensive Agar TEK [Re: verum subsequentis]
    #25343425 - 07/23/18 10:10 PM (5 years, 6 months ago)

Wow, not sure why I was afraid to start out with agar. Agar should be the only way to start this hobby. First agar plates were a success!


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InvisibleSarahBorealis
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Registered: 06/08/14
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Re: pour agar TEK [Re: PurePleasure]
    #25348063 - 07/26/18 12:25 PM (5 years, 6 months ago)

Thanks for all the info...I rarely reply because most of my questions are answered somewhere here.  My problem is that every time I've tried to introduce mycelium grown on agar I get a contaminated jar.  I use a SAB, bleached, a flame-sterilized blade, and I try to make sure everything is clean.  Mostly I get a fuzzy white contamination, sometimes green but not the deep green that usually gets on things.  I use both BRF and rye berries.  Trying oats next.  Any help is appreciated!  Also, what is your favorite casing?


--------------------
Somewhere, something incredible is waiting to be known.
For small creatures such as we the vastness is bearable only through love.
We're made of star stuff. We are a way for the cosmos to know itself.  Carl Sagan


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OfflineCthulhu23
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Re: pour agar TEK [Re: SarahBorealis]
    #25354658 - 07/29/18 07:41 PM (5 years, 5 months ago)

Bod, just wanted to say thank you for posting this (among many) comprehensive and easy to use TEK.  I used this information to build a SAB, but the necessary ingredients to make some BRF agar (left over from my first forey into amateur mycology via PF-TEK), pour plates, and clone pins from a B+ MS BRF cake.

This weekend while out in the woods with my kids we stumbled upon some Phoenix Oysters growing out of a downed log.


Took three separate aseptic (hopefully!) tissue samples and put them to agar. 


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Offlinemenacingtrees
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25356965 - 07/30/18 10:29 PM (5 years, 5 months ago)

So, I assume by "dry ingredients" you mean just the agar-agar powder?

You say you put that in the bottle and then fill with room temperature tap water till 400ml and then sterilize.

But where do you add the nutrients? I am going to use my grain soak water, but you never mention when to add that? wouldn't you add that instead of tap water?

Pardon me if I am misunderstanding but it doesn't seem very clear.


--------------------
"Just because it exists, doesn't mean it can't be done." - JG

Psilocybin plants your roots, LSD spreads your branches.
DMT turns your tree-ass into a damn rocket ship.


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's Comprehensive Agar TEK [Re: menacingtrees]
    #25357530 - 07/31/18 04:58 AM (5 years, 5 months ago)

What do you think is different about grain soak water ve regular water


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OfflineCthulhu23
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25357996 - 07/31/18 10:52 AM (5 years, 5 months ago)

Quote:

bodhisatta said:
What do you think is different about grain soak water ve regular water




Socratic questioning FTW!


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OfflineSleepful
Newbie pls help

Registered: 07/23/18
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Re: BOD's Comprehensive Agar TEK [Re: Cthulhu23]
    #25359445 - 08/01/18 02:53 AM (5 years, 5 months ago)

Can I do this without a thermometer? I'm wondering if that's really that difficult or if you can just eyeball the agar/temperature.

Another thing, are plastic petri dishes fine, and, can I reuse them?


Edited by Sleepful (08/01/18 02:54 AM)


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