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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: BOD's Comprehensive Agar TEK [Re: willhelm]
    #25070297 - 03/17/18 07:28 AM (5 years, 10 months ago)

for the brf agar i saw that you boil the water and just pour it into the bottle with the agar agar and brf.

is the procedure any different for no pour plates?


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Offlineomegaman
obsolete cyborg time traveler

Registered: 02/11/18
Posts: 26
Last seen: 5 years, 3 days
Re: pour agar TEK [Re: thelanzii]
    #25070850 - 03/17/18 12:34 PM (5 years, 10 months ago)

I know this is about agar. But when cloning do you seran wrap the plates


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: omegaman]
    #25070857 - 03/17/18 12:37 PM (5 years, 10 months ago)

As soon as possible


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OfflineToadwerd
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Registered: 03/16/18
Posts: 2
Last seen: 3 years, 2 months
Re: pour agar TEK [Re: bodhisatta]
    #25088384 - 03/24/18 10:23 PM (5 years, 10 months ago)

This is my first post, so take it for granted that I’m retarded.  I’ve read the TEK & all 21 pages & don’t see any conversation relating to the preferred size of the inoculating loop.  Does size matter?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: Toadwerd]
    #25088389 - 03/24/18 10:24 PM (5 years, 10 months ago)

Small enough to work i guess.


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OfflineToadwerd
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Registered: 03/16/18
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Re: pour agar TEK [Re: bodhisatta]
    #25089445 - 03/25/18 12:14 PM (5 years, 10 months ago)

Thx, Bod.  I’m completely new to this, but have spent dozens of hours over the past month researching technique & approach & have settled on taking your agar, oat spawn & monotub approach as my first rodeo.
Currently have 10q oat spawn one week into inoculation (having sterilized in my new Presto 23q pc & inoculated in my SAB of your design).
Being new, I have high hopes, & measured expectations. I am documenting photographically along the way & will post something as soon as I have anything to show.
Wanted to thank you for the time you have devoted to this hobby & to this forum.  Without knowledgeable people being willing to teach, the learning curve on this sort of thing would be insurmountable to most (myself included).
Thanks again!


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Invisiblevan hattonFacebookDiscord
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: pour agar TEK [Re: Toadwerd]
    #25091838 - 03/26/18 12:18 PM (5 years, 10 months ago)

Yo bod I was nuking a bottle minute ago and had a nice bubble pop in the agar.  A small amount of agar came between the threads and spilled down the bottle. 

Fucked or Naw?


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: van hatton]
    #25091948 - 03/26/18 01:05 PM (5 years, 10 months ago)

No not fucked.


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Invisiblevan hattonFacebookDiscord
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Re: pour agar TEK [Re: bodhisatta]
    #25092084 - 03/26/18 01:56 PM (5 years, 10 months ago)

Cool just finished pouring it :lol: thanks


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflineMad River
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Registered: 04/29/08
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Re: pour agar TEK [Re: van hatton] * 1
    #25095195 - 03/27/18 04:29 PM (5 years, 9 months ago)

So for the 400ml that you make in the agar  bottle, you'd use 8g each of agar agar and  BRF? Thank you.


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: Mad River]
    #25095199 - 03/27/18 04:31 PM (5 years, 9 months ago)

Yes


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OfflineMad River
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Re: pour agar TEK [Re: bodhisatta]
    #25095211 - 03/27/18 04:36 PM (5 years, 9 months ago)

Thanks again. Big fan.


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OfflineAma420
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Registered: 04/12/17
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Re: pour agar TEK [Re: Mad River]
    #25146234 - 04/17/18 06:05 PM (5 years, 9 months ago)

I just did my first spore print streak plate. As i was gathering spores from the print into the dish i had chunks fall out(extreme amount of spores in dish). Will i just get a lot of germination or is to many spores in 1 plate an issue?


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: pour agar TEK [Re: Ama420]
    #25146302 - 04/17/18 06:28 PM (5 years, 9 months ago)

the more spores the greater the chance of a contam and the more genetics, that's all


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OfflineAma420
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Registered: 04/12/17
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Re: pour agar TEK [Re: verum subsequentis]
    #25146332 - 04/17/18 06:39 PM (5 years, 9 months ago)

Quote:

verum subsequentis said:
the more spores the greater the chance of a contam and the more genetics, that's all



Sounds like more transfers than normal


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Offlineverum subsequentis
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Re: pour agar TEK [Re: Ama420]
    #25146418 - 04/17/18 07:07 PM (5 years, 9 months ago)

if your are trying to isolate. a lot more.


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OfflineAma420
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Re: pour agar TEK [Re: verum subsequentis]
    #25146430 - 04/17/18 07:11 PM (5 years, 9 months ago)

Glad i have 30 dishes poured and ready, another 500 ml worth of mea to pour. Thank you!!


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Edited by Ama420 (04/17/18 07:11 PM)


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InvisibleKimble
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Registered: 03/08/18
Posts: 508
Re: pour agar TEK [Re: Ama420]
    #25152807 - 04/20/18 08:39 AM (5 years, 9 months ago)

Anyone experience colored agar returning to normal color after pc cycle? I'd love to have some colored plates but it keeps happening. Probably the brand of food color. I wonder if there is some lab grade dye I could use?


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InvisiblebodhisattaMDiscordReddit
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Re: pour agar TEK [Re: Kimble]
    #25153039 - 04/20/18 10:14 AM (5 years, 9 months ago)

I would just skip food coloring all together honestly


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InvisibleKimble
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Registered: 03/08/18
Posts: 508
Re: pour agar TEK [Re: bodhisatta]
    #25153100 - 04/20/18 10:54 AM (5 years, 9 months ago)

Yeah, fair enough to be honest. Also for anyone else reading this, I'm finding I can pour it much cooler than the literature says to. According to Google it solidifies between 32 and 40c. I'm getting mad condensation even down to around 42c and it isn't solidifying until... Below 34c according to my infrared thermometer.
I'm guessing the actual liquid is warmer than the outside of the media bottle and that is why the temps seem off. Get lowwwww...


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