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InvisiblebodhisattaM
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Re: pour agar TEK [Re: drgreyskull]
    #25033278 - 03/02/18 10:35 AM (2 years, 5 months ago)

Quote:

drgreyskull said:
Sorry I don't mean to hijack your thread with dumb questions. So cut open, and try to cut out a little sliver the size of a grain of rice?  Then drop in the middle of dish?



Yep thats what cloning is..


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InvisibleAyePlus
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Re: pour agar TEK [Re: bodhisatta]
    #25033292 - 03/02/18 10:39 AM (2 years, 5 months ago)

:whathesaid:
Except rip open instead of cut open, the blade can drag contaminants in.

Invitro plate pins make good clones too but generally this is how its done.


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Invisiblethelanzii
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Re: pour agar TEK [Re: AyePlus]
    #25040739 - 03/05/18 03:21 PM (2 years, 5 months ago)

i am confused about the brf agar recipe

how much brf am i using per/1000ml

how much agar-agar am i using per/1000ml

is this with distilled water or spring water

also i plan on pre pouring in half pint glass dishes

how long would i pressure cook for?

how many ml per dish?


Edited by thelanzii (03/05/18 03:30 PM)


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Offlineverum subsequentis
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Re: pour agar TEK [Re: thelanzii]
    #25040758 - 03/05/18 03:30 PM (2 years, 5 months ago)

i use tap water at 20 grams agar per liter. don't know about brf because i've never used it. MEA for me baby. at 20 to 30 grams per liter.


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Invisiblethelanzii
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Re: pour agar TEK [Re: verum subsequentis]
    #25040759 - 03/05/18 03:31 PM (2 years, 5 months ago)

tempted to just buy some of that


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OfflinemushboyM
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Re: pour agar TEK [Re: thelanzii] * 1
    #25040769 - 03/05/18 03:34 PM (2 years, 5 months ago)

brf agar kicks ass

12g brf per liter
18g-20g agar per liter

i just use tap water. ive used distilled,spring, mineral notice no difference:shrug:


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Invisiblethelanzii
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Re: pour agar TEK [Re: mushboy]
    #25040780 - 03/05/18 03:39 PM (2 years, 5 months ago)

how many ml's of brf agar go into a pasty plate?

I am using half pint glass jars but they appear to be about the same volume


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OfflinemushboyM
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Re: pour agar TEK [Re: thelanzii]
    #25040786 - 03/05/18 03:40 PM (2 years, 5 months ago)

little as possible.



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InvisibleChanga Alchemist
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Re: pour agar TEK [Re: mushboy]
    #25040939 - 03/05/18 04:39 PM (2 years, 5 months ago)

I do 8g brf and 10g agar for 500ml.


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Offlineomegaman
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Re: pour agar TEK [Re: Changa Alchemist]
    #25042263 - 03/06/18 07:12 AM (2 years, 5 months ago)

two questions. first if you have spore prints do you need to add them to water before making a streak plate? or will dry spore prints be preferable?
second. what should be the environment for the plates during germination? lights,darkness,temps?


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InvisibleAyePlus
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Re: pour agar TEK [Re: omegaman]
    #25042358 - 03/06/18 08:31 AM (2 years, 5 months ago)

No, just wipe the print with a loop or sterilised swab and then wipe the agar

Room temp~ 70, Lights dont really matter


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Invisibleboom boom

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Re: pour agar TEK [Re: AyePlus]
    #25042778 - 03/06/18 11:26 AM (2 years, 5 months ago)

Edit: this question was just asked and answered and I didn't see it. My bad.


Edited by boom boom (03/06/18 11:27 AM)


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InvisibleiSMOKErocks
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Re: pour agar TEK [Re: boom boom]
    #25047343 - 03/07/18 08:30 PM (2 years, 5 months ago)

https://www.ebay.com/itm/Pyrex-Slimline-Media-Bottle-Easy-Pour-Corning-500ML-Glass/122994449302?ssPageName=STRK%3AMEBIDX%3AIT&_trksid=p2057872.m2749.l2649

Damn they just ended a 50% off sale got 2 bottles for 16$ Free ship !,, eBay rules!
I was pouring plates using my wide mouth quart jar as a vessel....fucking spilled hot agar everywhere hahaha got so mad cuz i lost it alI!! let it cool and peeled it up like a pancake lol,,,,, reminded me about getting one of these sexy Pyrex bottles from this tek,,,, can't wait to have nice things  :hairmetal:


--------------------
:freakout:  " Being challenged in life is inevitable being defeated is optional."


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: iSMOKErocks]
    #25049815 - 03/08/18 03:57 PM (2 years, 5 months ago)

That's a good price on the reguar 500ml ones let alone the slim style. I want to try them but no real reason to waste money on em i got a bunch of regular shape media bottles anyway


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Invisiblethelanzii
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Re: pour agar TEK [Re: bodhisatta]
    #25049827 - 03/08/18 04:02 PM (2 years, 5 months ago)

why do people not shake the agar wedges once incoculated


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Offlinefesh
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Re: pour agar TEK [Re: thelanzii]
    #25049845 - 03/08/18 04:08 PM (2 years, 5 months ago)

Very nice I'm cloning some fruits right now


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OfflinemushboyM
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Re: pour agar TEK [Re: fesh]
    #25049960 - 03/08/18 04:52 PM (2 years, 5 months ago)

when i was new to a2g, i wouldnt shake because i wanted to see where my contams may be coming from.
after i was certain and around 30% colonized id shake em up and another week or so they are good 2 go.


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Offlinedrgreyskull
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Re: pour agar TEK [Re: mushboy]
    #25051831 - 03/09/18 12:15 PM (2 years, 5 months ago)

Ok so after I grew some plates I realized I have no fucking idea what I'm doing ha.
Here are 4 plates I transferred from I couldn't get a good picture unless it was open.

I followed another Tek and cut pieces from what I thought was good.


Edited by drgreyskull (03/09/18 12:17 PM)


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Offlinejblue1408
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #25064910 - 03/15/18 08:50 AM (2 years, 4 months ago)

Nice Read...


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Offlinewillhelm
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Re: BOD's Comprehensive Agar TEK [Re: jblue1408]
    #25070274 - 03/17/18 07:09 AM (2 years, 4 months ago)

Just wanted to say I got myself a water bath because of this thread, and boy does it make pouring plates more pleasurable


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