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Offlineshroomflow
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Re: pour agar TEK [Re: AyePlus]
    #24937619 - 01/24/18 12:21 AM (2 years, 7 months ago)

Another dope ass TEK, thanks BOD. Can't wait to stop doing syringes.


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Offlinem.smith
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Re: pour agar TEK [Re: shroomflow]
    #24980320 - 02/10/18 09:28 AM (2 years, 7 months ago)

Medium term storage question???

I read what you said about long term storage, what about Medium Term?

How long is an un-refrigerated dish good for?

I made about 40 plates just after Christmas 2017, it took about three weeks for them to become fully colonized. They've been stored in zip lock bags in a 68 degree closet for two months. I used food coloring and they stayed nicely dyed for the first month, the second month the dye magically went away, maybe the mycelium ate the dye? They are now starting to look a bit dry.

Can I just throw them all in a insulated lunch box in the fridge and call them good for a year?

Thanks again Bod, I successfully used your tek's to go from complete noob to successfully going from spore syringe to agar, to grain, to bulk mono tub to harvest and my first trip. You are a hero in my book :smile:


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InvisibleMateah
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Re: pour agar TEK [Re: m.smith]
    #24980338 - 02/10/18 09:43 AM (2 years, 7 months ago)

Thinly poured agar will not last a year inside the fridge, it will most likely dry out much faster than that.

For medium storage you would need at least very thick poured plates or culture tubes with agar, aka slants. p

You will need more agar mass for it not to dry out that fast :thumbup:


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Onlineverum subsequentis
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Re: pour agar TEK [Re: m.smith]
    #24980683 - 02/10/18 12:39 PM (2 years, 7 months ago)

You could throw it in the fridge and bring it back to life a year later. It IS possible but isn't worth the risk of loosing it. If it's already looking dry I'd pour new thick plates and transfer into those, let that grow out a bit, and then fridge it. Or get some slants. Or use a little tiny Mason jar with thick agar.


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Re: pour agar TEK [Re: verum subsequentis]
    #25003008 - 02/18/18 09:41 AM (2 years, 6 months ago)

This may seem like a crazy question but I have some B+ growing now. I get paid in a couple of days and want to clone one of them. Can I pick the mushroom and keep it good untill my agar comes then clone?


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Re: pour agar TEK [Re: drgreyskull]
    #25003128 - 02/18/18 11:04 AM (2 years, 6 months ago)

Throw it in the fridge. Should be fine for 3-4 days, probably longer.


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Re: pour agar TEK [Re: AyePlus]
    #25003193 - 02/18/18 11:44 AM (2 years, 6 months ago)

I have been reading about cloning for awhile now and it has a!ways been intimating. When picking a fruit to clone I am just looking for one that looks healthy, big and has no mutations? Do you recommend taking samples from multiple fruits or keeping it simple and just a couple or even just one? Sorry for all the questions this fascinates me and I really want to get into growing aand perfecting cultures.


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: drgreyskull] * 1
    #25003207 - 02/18/18 11:54 AM (2 years, 6 months ago)

Start somewhere and get better. Don't worry about perfection right now. Any clone is going to be a step in the right direction


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Re: pour agar TEK [Re: bodhisatta]
    #25003226 - 02/18/18 12:02 PM (2 years, 6 months ago)

Biggest fruit from the earliest dense cluster is usually my first choice.


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Offlinedrgreyskull
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Re: pour agar TEK [Re: bodhisatta]
    #25003317 - 02/18/18 12:52 PM (2 years, 6 months ago)

Quote:

bodhisatta said:
Start somewhere and get better. Don't worry about perfection right now. Any clone is going to be a step in the right direction




thanks, im going to wait to do my monotubs and focus on learning to clone for now.

I have a brewing store in my town was going to look there to buy the ME but I was thinking about the agar is there a chance i can get it at walmart or some grocery store?


Edited by drgreyskull (02/18/18 12:54 PM)


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InvisibleTrav420
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Re: pour agar TEK [Re: drgreyskull]
    #25021989 - 02/25/18 11:28 PM (2 years, 6 months ago)

Is there any other ways of preventing the agar from boiling over when PCing besides just filling the water up to the level of the agar in the bottle?  I've made agar twice now following this tek and both times I've gotten just a slight boil over.  It wasn't much but still kinda annoying. This is my first time doing this so I just wanted to check. I'm using the same size agar bottle (500mL) as well if that makes any difference.


--------------------
Simplicity of character is the natural result of profound thought...
Your thoughts create your life...
“Waste no more time arguing about what a good man should be. Be one.”  Marcus Aurelius


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Trav420]
    #25022532 - 02/26/18 07:25 AM (2 years, 6 months ago)

You're cooling down to quickly


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OfflinePostChase
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Re: pour agar TEK [Re: drgreyskull]
    #25022671 - 02/26/18 09:06 AM (2 years, 6 months ago)

Quote:

drgreyskull said:


thanks, im going to wait to do my monotubs and focus on learning to clone for now.

I have a brewing store in my town was going to look there to buy the ME but I was thinking about the agar is there a chance i can get it at walmart or some grocery store?





Yes, you should be able to get it at any asian grocery store, or in most grocery stores in general. I find the best deals at the asian groceries though.


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Re: pour agar TEK [Re: bodhisatta]
    #25023479 - 02/26/18 01:16 PM (2 years, 6 months ago)

Ah ok.  I've been just turning the stove off once its done and leaving it sit there until the pressure comes off, not trying to rush it or anything.  My house is old and cold so should I try wrapping a towel or something around it to help slow the cool down maybe?  Total noob to this so forgive my ignorance on the matter.  On the plus side the agar turned out great, exactly like the tek except lots of condensation in the plates which is I guess to be expected due to the less then tropical conditions I have to live with...lol


--------------------
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Your thoughts create your life...
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Offlinedrgreyskull
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Re: pour agar TEK [Re: AyePlus]
    #25033055 - 03/02/18 11:02 AM (2 years, 6 months ago)

Quote:

AyePlus said:
Throw it in the fridge. Should be fine for 3-4 days, probably longer.



i did this and im about to start my agar now. i was reading that it is better to use the pins so I threw a cake in my SAB and cut 4 pins off put them in a bag and in my myco fridge. when i look at them they dont look right the bottoms are purplish are they just bruised from handling? and can they get to cold?



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InvisibleAyePlus
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Re: pour agar TEK [Re: drgreyskull]
    #25033082 - 03/02/18 11:15 AM (2 years, 6 months ago)

Pins from clean agar dishes are good for clones, pins from BRF cakes or bulk  are questionable since brf cakes are usually not fruited aspetically chances are theyre covered in spores and bacteria. So any clone from bulk should be inner tissue. Only time pins are clean are when they pin invitro.

Theyre probably just bruised from handing. Hope they're big enough to get some inside tissue from.


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Offlinedrgreyskull
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Re: pour agar TEK [Re: AyePlus]
    #25033202 - 03/02/18 12:06 PM (2 years, 6 months ago)

shit, they are tiny, I have no idea if I could get inner tissue from them. I will try but I don't think it's going to happen. Here is a pic any ideas.
(thumb and spore print for reference.


"Only time pins are clean are when they pin invitro."- Meaning in the jar?


Edited by drgreyskull (03/02/18 12:09 PM)


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: drgreyskull]
    #25033209 - 03/02/18 12:08 PM (2 years, 6 months ago)

Those are plenty big enough lol...


You could make ten dishes from each fruit


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Offlinedrgreyskull
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Re: pour agar TEK [Re: bodhisatta]
    #25033254 - 03/02/18 12:26 PM (2 years, 6 months ago)

Sorry I don't mean to hijack your thread with dumb questions. So cut open, and try to cut out a little sliver the size of a grain of rice?  Then drop in the middle of dish?


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InvisibleAyePlus
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Re: pour agar TEK [Re: drgreyskull]
    #25033268 - 03/02/18 12:31 PM (2 years, 6 months ago)

Invitro means grown entirely inside a container, jar, bag, petri, whatever. But not exposed to open air ever. Also sporeprints and thumbs dont make the best size reference because they aren’t standard sized. In this case theyre good enough but in the future; A coin or a bic lighter work better.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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