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Onlineverum subsequentis
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Re: pour agar TEK [Re: bodhisatta]
    #24745412 - 10/29/17 02:02 PM (2 years, 10 months ago)

That's what she said


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InvisibleGerms
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Re: pour agar TEK [Re: verum subsequentis]
    #24745425 - 10/29/17 02:09 PM (2 years, 10 months ago)

I was going to :whatshesaid: until I clicked next page and saw I was beat


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Offlinebeancake
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24746762 - 10/30/17 03:09 AM (2 years, 10 months ago)

OK so you wait for pin to form, then you transfer from the leading edge of myc that produced the pin?

Then repeat until you have what appears to be single growth. Then do a grow? If there is a standout fruit start process all over with biopsy? 

Quote:

bodhisatta said:
[url=/forums/showflat.php?Cat=0&Number=24297804&page=0&vc=1#24297804]


more like this

and move those pins to agar like the pictures above.
the little pins grow very quickly and vigorously, the pins from another petri dish are going to have no contamination on them so cloning is even easier than taking a biopsy from a fruit growing in a tub. Biopsy clones are perfectly fine too.





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InvisiblebodhisattaM
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Re: pour agar TEK [Re: beancake]
    #24747189 - 10/30/17 11:09 AM (2 years, 10 months ago)

Quote:

beancake said:
To confirm, finding clone from MS:

1. Biopsy large fast growing fruit to agar
2. Move pin(s) from agar to new dish
3. Repeat step 2???
4. Test grow

Once you have found your clone will spores be clones as well?



Yes but you said finding a clone from MS. I figured a MS grow.

Either way still wasn't right

Step one put the clone biopsy(from the pinning plate) to agar(a new plate).

Step two. That biopsy grows. It STAYS on that plate. The mycelium that grows out from the clone biopsy moves to a new plate.

Step 3. Keep transferring mycelium. Don't ever pick the biopsy back up.

Literally do what makes sense


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Offlinebeancake
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Re: pour agar TEK [Re: bodhisatta]
    #24748121 - 10/30/17 06:55 PM (2 years, 10 months ago)

To find clone from MS do you first fruit and take a biopsy from shroom to agar to select pin, or spores straight to agar to pin?

Once agar has pin, take from leading edge around that pin to new plate and repeat...

One of my questions was, once I decide to do a grow from selected clone from several generations of agar plates, if a particular shroom stands out (faster growing/larger whatever) would I biopsy it and start the process over. I believe you are saying "no" stick with agar.


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Onlineverum subsequentis
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Re: pour agar TEK [Re: beancake]
    #24748204 - 10/30/17 07:31 PM (2 years, 10 months ago)

sounds like you need to read a bit.

https://www.shroomery.org/forums/dosearch.php?forum%5B%5D=&words=ms+to+agar&namebox=&expert=1&replybox=&how=all&where=body&tosearch=both&newerval=&newertype=y&olderval=&oldertype=y&minwords=&maxwords=&limit=25&sort=r&way=d

there you go. That's every time a trusted cultivator has ever said "ms to agar". Searches like that will lead you to glory.


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: beancake] * 1
    #24748210 - 10/30/17 07:32 PM (2 years, 10 months ago)

You can get a MS pin either way.

To clone something you move the mushroom if you're cloning from a petri dish move the whole pin
If you're cloning from a grow, take a biopsy.

The initial plate will have either a pin or a biopsy in the middle

You take mycelium from the leading edge of that plate to a new llate leaving the clone tissue or pin behind.

Keep making transfers if needed to get a clean culture.

I guess i wasn't clear enough. It should just be clear by virtue of what your trying to accomplish.


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Offlinebeancake
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Re: pour agar TEK [Re: bodhisatta]
    #24749339 - 10/31/17 10:08 AM (2 years, 10 months ago)

Thank you for clarification on your process.


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Re: pour agar TEK [Re: beancake]
    #24750036 - 10/31/17 04:02 PM (2 years, 10 months ago)

I did the BRF agar yesterday and cloned to six plates, i waited to long to pick so the ones that i was eyeing to clone were too mature and had dropped spores but i picked a suitable replacement. I feel i did pretty well pouring the plates, but i struggled when doing the tissue transfer. The method described is a scraping motion with the scalpel but i just couldn't get it to stick, i felt i was touching it far too much with my gloves, but none the less that shit was fun and im stoked to keep practicing. Im sure this is a "easier with time" type of skill so im not discouraged. I have more syringes that i am just gonna noc more cakes up with, do u recommend streaking some plates just for practice or wait until i know i have a fruiting strain and just clone. cause from the sounds of it MS is kinda hit or miss and the cloning is the tried and true way to get good croppers.

Thanks for all these beautiful write ups BOD.


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Re: pour agar TEK [Re: thewiseguywithaj]
    #24764192 - 11/06/17 05:13 PM (2 years, 10 months ago)

Question.

I was reheating my media bottle and it had a slight boilover would you still use that or naw?


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: pour agar TEK [Re: van hatton]
    #24764216 - 11/06/17 05:21 PM (2 years, 10 months ago)

Probably


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ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Invisiblevan hatton
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Re: pour agar TEK [Re: bodhisatta]
    #24764240 - 11/06/17 05:30 PM (2 years, 10 months ago)

Thanks man


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: pour agar TEK [Re: bodhisatta]
    #24764951 - 11/06/17 10:19 PM (2 years, 10 months ago)



Had issue with condensation pouring these plates. Today Agar to agar transferred the second pic plate that had the large dark visible contam spot. This tissue plate had the best looking growth. Where the growth looks like frayed thread is where i cut wedges from. Is that what it looks like when you hear the term "rhizomorphic growth"?

was this too early to be transferring? it was only a week but i wanted to save it from the contam because it was growing so nice

Also does it look like there are two different contam colonies in the second pic? the dark one and then like a cloudy one?


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Onlineverum subsequentis
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Re: pour agar TEK [Re: thewiseguywithaj] * 1
    #24771456 - 11/09/17 03:21 PM (2 years, 10 months ago)

yes.
no.
yes.


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Re: pour agar TEK [Re: verum subsequentis]
    #24789016 - 11/17/17 11:18 AM (2 years, 9 months ago)

When you use the water from your oat prep. Do you do it all in the same day or can I just save it in the fridge for when I'm ready to make some agar plates??


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Re: pour agar TEK [Re: Pinball24]
    #24789043 - 11/17/17 11:33 AM (2 years, 9 months ago)

It will eventually go bad in the fridge its got a 3-4 day shelf life like any unpasteurized fresh juice


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OfflineSkizor1337
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Re: pour agar TEK [Re: bodhisatta]
    #24789101 - 11/17/17 12:11 PM (2 years, 9 months ago)

Only part that gets me is where those little mycelium bits when are we supposed to go in the petting dishes. Also where is that from?


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OfflineJFlint

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Re: pour agar TEK [Re: Skizor1337]
    #24813688 - 11/29/17 08:28 AM (2 years, 9 months ago)

Bod, using cling wrap or similar cellophane product, would you not be concerned with gas exchange sealing up your dishes like that? I’d like to use some half pint jars for a no pour trial with agar, but I would prefer to use unmodified lids. If GE is not a concern then I’ll definitely give that a go.


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Re: pour agar TEK [Re: JFlint]
    #24864120 - 12/23/17 05:04 PM (2 years, 8 months ago)

Quick question for bod or anyone else. I get lots of boil over while my agar is pc'ing. I use 1L erlenmyer flask. I've tried it full, half full, etc. I always lose about 60%-80% agar by the time I remove it. I use Mycomedia from fungi perfecti which I believe to be a malt agar. It's recommended 50g powder to 1L water. setting, growing, etc are all perfect and I enjoy using it, just damn boil over is killing me.
I mix, put tin foil over the lid. I've tried putting the flask in the pc while the water heats up and also placing it in pc just before I put the lid on and tighten, both boiled over. I PC at 15psi for 40-45m as recommended. I wait 1-2 hours to let the pc and agar cool together before even touching the pc. Then I open in front of my flow hood. The boil over happens early into the PC because it gets all over the inside of my pc and burns. Is the pc too hot? too long? I've tried with a silicone cork in the top of tubes I pc'd for slants and they popped the top within 10 minutes of the pc (I could hear them pop). I've filled water up to the same level of agar on inside of flask. Not sure what i'm missing. Thoughts?


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Re: pour agar TEK [Re: Nephrim]
    #24864269 - 12/23/17 06:13 PM (2 years, 8 months ago)

40-45 minutes is long. Try 20-25 minutes at 15 psi


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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