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InvisibleGerms
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Re: BOD's Comprehensive Agar TEK [Re: Severance13]
    #24569788 - 08/21/17 05:48 PM (3 years, 29 days ago)

Nest scientific has 500 for $87 plus shipping I believe. They had a deal on Amazon recently and I just got 500 for $47. Couldn't let it pass up lol


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InvisibleMycolorado
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Re: BOD's Comprehensive Agar TEK [Re: Germs]
    #24569824 - 08/21/17 06:07 PM (3 years, 29 days ago)

Hey Germs, How's the quality on those nest sci plates?  I saw that deal and was debating getting a case...I use falcons as you can tell the difference in quality...really does make a difference, but they are super pricey. :eek:


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InvisibleGerms
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Re: BOD's Comprehensive Agar TEK [Re: Mycolorado]
    #24569833 - 08/21/17 06:11 PM (3 years, 29 days ago)

They've been good so far, poured my 4th sleeve of them today. I don't like that they're vented, I've had two out of the first 60 get tiny satellite molds on the edge without me doing anything to them. They're stackable and have great clarity so for the price I'm more than pleased. Only two of the whole case broke in transit.


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InvisibleMycolorado
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Re: BOD's Comprehensive Agar TEK [Re: Germs]
    #24569835 - 08/21/17 06:13 PM (3 years, 29 days ago)

Thanks for the input!:thumbup:


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Offlinel2en
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Re: BOD's Comprehensive Agar TEK [Re: Severance13]
    #24580129 - 08/26/17 01:38 AM (3 years, 25 days ago)

Quote:

Severance13 said:
One very important question: where do you get your petris? I can't find anywhere close to that price. Even Aliexpress and spectrum scientific is around $102 for 500 100x15mm plates




http://www.ebay.com/itm/Nest-Scientific-753001-Polystyrene-Petri-Dish-Semi-Stackable-Sterile-100-mm-/302427992823?epid=2169284889&hash=item466a1cf2f7:g:IzsAAOSwB31ZoNtj

.99 plus 29.35 for shipping


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Offlinethewiseguywithaj
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Re: pour agar TEK [Re: l2en]
    #24627184 - 09/13/17 08:44 PM (3 years, 6 days ago)

Once its made and stored in the fridge do u need to loosen lid before microwaving and returning to liquid?


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You're all the same the lot of you. With your long hair and faggot clothes. Drugs, Sex, every kind of filth. And you hate the police don't you?

YOU MAKE IT EASY!!!

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InvisibleGerms
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Re: pour agar TEK [Re: thewiseguywithaj] * 1
    #24627415 - 09/13/17 10:39 PM (3 years, 6 days ago)

Quote:

thewiseguywithaj said:
Once its made and stored in the fridge do u need to loosen lid before microwaving and returning to liquid?



I would if you don't want a vacuum shattered jar with agar all over your microwave


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Offlinethewiseguywithaj
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Re: pour agar TEK [Re: Germs]
    #24629627 - 09/14/17 07:21 PM (3 years, 5 days ago)

That's what I figured it just didn't specify.

Where do u usually find those media container bottles? amazon maybe?


--------------------
You're all the same the lot of you. With your long hair and faggot clothes. Drugs, Sex, every kind of filth. And you hate the police don't you?

YOU MAKE IT EASY!!!

the wizardddddd in BLACK


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: thewiseguywithaj]
    #24629678 - 09/14/17 07:31 PM (3 years, 5 days ago)

Quote:

then into the microwave with the cap very loose
bring to a boil. careful don't let it boil over and make a fucking huge mess.
I find it best to not walk away during this step, just keep an eye on it.




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police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Offlinethewiseguywithaj
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Re: pour agar TEK [Re: bodhisatta]
    #24629720 - 09/14/17 07:39 PM (3 years, 5 days ago)

Oh yea right on BOD i read that part. But that was about forming the agar in the first place. My question was regarding using the agar after being stored in the fridge and returning it to liquid state since its not being boiled. I should have assumed. The fear of contams is REAL! haha


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: thewiseguywithaj] * 1
    #24629724 - 09/14/17 07:42 PM (3 years, 5 days ago)

it is being boiled. you basically have to to get it back to liquid. there will still be some unmelted mass in there while the rest of it hits a boil


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Offlinethewiseguywithaj
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Re: pour agar TEK [Re: bodhisatta]
    #24629855 - 09/14/17 08:36 PM (3 years, 5 days ago)

Hence the shaking and returning to microwave. Makes sense, Thanks


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Offlineicetech
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Re: pour agar TEK [Re: thewiseguywithaj]
    #24677274 - 10/02/17 09:03 AM (2 years, 11 months ago)

Just starting to learn about Agar and am curious why you first add ingredients then boil in the MW, and then PC it.. wouldn't just using the PC do the same thing?


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InvisibleGerms
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Re: pour agar TEK [Re: icetech]
    #24677283 - 10/02/17 09:09 AM (2 years, 11 months ago)

You’ll get mixed answers on that one. From what I know it’s standard lab practice to dissolve the ingredients in boiling water but some phD on here says its not needed and is a waste of time.


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Re: pour agar TEK [Re: Germs]
    #24677296 - 10/02/17 09:22 AM (2 years, 11 months ago)

Quote:

Germs said:
You’ll get mixed answers on that one. From what I know it’s standard lab practice to dissolve the ingredients in boiling water but some phD on here says its not needed and is a waste of time.




thank you for the info :smile:


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Germs]
    #24677407 - 10/02/17 10:42 AM (2 years, 11 months ago)

Quote:

Germs said:
You’ll get mixed answers on that one. From what I know it’s standard lab practice to dissolve the ingredients in boiling water but some phD on here says its not needed and is a waste of time.



Someone who has "talked to a phd" lol



--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Offlinetripdawg420
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Re: pour agar TEK [Re: bodhisatta]
    #24678331 - 10/02/17 04:58 PM (2 years, 11 months ago)

:takingnotes:


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Offlinethewiseguywithaj
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Re: BOD's Comprehensive Agar TEK [Re: Germs]
    #24683301 - 10/04/17 02:56 PM (2 years, 11 months ago)

On the streak plates there are a couple pics u have with dark spots is that contam? then u just want to cut off that and avoid the dark spots?

Also i see streaking is the preferred method to just squirting from a syringe when using spore to agar, would the reason be just because it is neater?


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InvisiblebodhisattaM
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Re: BOD's Comprehensive Agar TEK [Re: thewiseguywithaj]
    #24683341 - 10/04/17 03:16 PM (2 years, 11 months ago)

Its soot or globs of spores

Syringe right to agar and you get all growth contamination and mushroom from the same spot


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Offlinethewiseguywithaj
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24683673 - 10/04/17 05:20 PM (2 years, 11 months ago)

ah i see so the streaking increases the solution surface area of the spores so contam and mushroom will be easier to separate


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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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