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InvisiblebodhisattaM
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Re: pour agar TEK [Re: BodhiBoy]
    #24435551 - 06/26/17 12:05 AM (3 years, 2 months ago)

smell it, I would make new stuff but it is probably OK


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police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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InvisiblePipefitter537
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Re: pour agar TEK [Re: bodhisatta]
    #24437957 - 06/26/17 11:46 PM (3 years, 2 months ago)

Gonna try to make some slants and was wondering what the best agar recipe would be, if there is one. I've been making pda and BRF agar lately.


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"You should never underestimate the predictability of stupidity"-Bullet Tooth Tony




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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Pipefitter537]
    #24438035 - 06/27/17 12:18 AM (3 years, 2 months ago)

Any agar recipe that works well works for slants


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police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Re: pour agar TEK [Re: bodhisatta]
    #24438522 - 06/27/17 07:44 AM (3 years, 2 months ago)

:takingnotes:


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Re: pour agar TEK [Re: oontribe]
    #24440328 - 06/27/17 08:39 PM (3 years, 2 months ago)

Can I sterilize the petri dishes on a mini convection oven, i.e. an air fryer?? :barbershreds: 

my oven is broken


Edited by FckingNameless (06/27/17 08:40 PM)


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: FckingNameless]
    #24440337 - 06/27/17 08:43 PM (3 years, 2 months ago)

Glass ones.


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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OfflineAkbal5
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Re: pour agar TEK [Re: bodhisatta]
    #24445244 - 06/29/17 07:11 PM (3 years, 2 months ago)

:takingnotes::congrats: :yourock:


Edited by Akbal5 (06/29/17 07:13 PM)


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OfflineTreasureCoastfunga
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24446616 - 06/30/17 11:15 AM (3 years, 2 months ago)

What am I doing wrong, I've followed the recipe twice and my agar always comes out soupy, am I not using enough agar? 10g light malt extract/10g agar agar w/ 500 ml water, sound good? And then, the agar (after inoculation) is stored room temp right?


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InvisiblebodhisattaM
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Re: BOD's Comprehensive Agar TEK [Re: TreasureCoastfunga]
    #24446632 - 06/30/17 11:27 AM (3 years, 2 months ago)

Does it stay soupy even at room temperature?


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police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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OfflineBodhiBoy
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24446920 - 06/30/17 02:05 PM (3 years, 2 months ago)

Yo this microwave tech is the shit but for real it can bubble over faster than bad taco bell going right through ya

I left mine in the cooker overnight to cool....hopefully reliquifying them isn't too tricky


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OfflineTreasureCoastfunga
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24447144 - 06/30/17 03:44 PM (3 years, 2 months ago)

Honestly, I must've just messed up the measurements, it's the only thing I can think of after having asked the question and reading, I'll try it again tonight and double check the scale to make sure I'm on the right settings


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Re: pour agar TEK [Re: TreasureCoastfunga]
    #24498245 - 07/21/17 11:11 AM (3 years, 1 month ago)

how long do you pc in total if you dont have a microwave?  it says 20 m in pc after bringing to a boil in microwave?  thanks


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Scalerunner]
    #24498253 - 07/21/17 11:16 AM (3 years, 1 month ago)

You don't need to adjust the time


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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InvisibleGerms
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Re: pour agar TEK [Re: bodhisatta]
    #24498267 - 07/21/17 11:26 AM (3 years, 1 month ago)

I pour boiling water in the pyrex bottle after adding the ingredients to whatever amount like 400ml then cap on and shake the hell out of it. The window of my microwave that's brand new still sucks ass so I can't really tell if it's boiling


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Germs]
    #24498279 - 07/21/17 11:32 AM (3 years, 1 month ago)

I only boil it first to make myself feel better


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police violence is no accident
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There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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OfflineBmason2323
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Re: pour agar TEK [Re: bodhisatta]
    #24538605 - 08/08/17 09:00 AM (3 years, 1 month ago)

Hey bro sorry for asking you probably get this alot, but ive been absolutely been loving your agar tek and i was just wondering when you pc the agar in your nedia bottle what do you mean by bring the water up to the agar? Do you mean fill your pc all the way up until the water submerges the whole bottle and get it level with the agar or do you mean just level with the bottom of the glass. Thanks heaps in advance mate


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Bmason2323]
    #24538607 - 08/08/17 09:02 AM (3 years, 1 month ago)

Neither. Until it reaches the level of the agar in the bottle.
So somewhere between just touching the bottom and being less than submerged. You don't want the cap under water.



--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Re: pour agar TEK [Re: bodhisatta]
    #24538627 - 08/08/17 09:19 AM (3 years, 1 month ago)

Sweet thanks heaps mate :smile: legend


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InvisiblebodhisattaM
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24564593 - 08/19/17 11:03 AM (3 years, 1 month ago)

:hi:


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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OfflineSeverance13
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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #24569658 - 08/21/17 04:53 PM (3 years, 29 days ago)

One very important question: where do you get your petris? I can't find anywhere close to that price. Even Aliexpress and spectrum scientific is around $102 for 500 100x15mm plates


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