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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis] * 1
    #24208516 - 03/31/17 05:11 PM (3 years, 5 months ago)

It's a lot of work to test isolates, especially when you test them full scale to see if they do well with your grow method

https://www.shroomery.org/forums/showflat.php/Number/18219379/fpart/all/vc/1

Or he could do one MS tub then clone a big dick and have ten tubs look like that a few weeks later


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Re: pour agar TEK [Re: bodhisatta]
    #24208649 - 03/31/17 06:10 PM (3 years, 5 months ago)

Yeah. I agree that it's a lot of work. But I think it might be worth it in the end. Just finding the "Knock your socks off" sub strain is a cool and rewarding idea. I'm about 65 plates into it with PE ms and just became curious when i saw that water running over the sectors. It seems to make sense that the water could carry little bits of myc and possibly do away with further isolation. just thought i'd ask.
    I also agree that a ms clone is totally easy, quick, and acceptable. I just thought i'd give a first generation pure isolate a try this time. It's a fun experiment while I have about 30 different ms and clone jars, and two bags colonizing now, to fruit and clone the keepers. Mostly from syringe to agar to grain but a couple ms syringe to grain.

  EDIT- oh yeah and a couple clone to agar to grain.

And yeah, I've checked out that pussy fart thread before. A lot of work indeed but I'd just test all the isolates out in little trays in a shotgun really quick instead of committing full monos.


Edited by verum subsequentis (03/31/17 06:19 PM)


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis]
    #24208674 - 03/31/17 06:24 PM (3 years, 5 months ago)

Betcha my good clone is 98% as good as a stellar isolate.

Water on a plate is more of a worry for carrying around bacteria or spores from simple molds but I guess maybe just maybe it could hamper isolation effort on occasions.

If you want an isolate though go for it but i definitely would get a limited MS grow and a clone going first


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Re: pour agar TEK [Re: verum subsequentis]
    #24208717 - 03/31/17 06:38 PM (3 years, 5 months ago)

i bet you have a very sexy clone. the pictures in your tag (signature or whatever you call it) are beautiful. I have a couple clones going at the moment and am super excited to compare. I actually tried my hand at cloning a 6 month old dried dick and had great success as well. I was excited about that. thanks again man.


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis]
    #24208737 - 03/31/17 06:42 PM (3 years, 5 months ago)

You'll do great. You're asking the right questions


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Re: pour agar TEK [Re: bodhisatta] * 1
    #24208786 - 03/31/17 06:59 PM (3 years, 5 months ago)

RR posted from ideal standpoints. Ideally it's even better to obtain 2 dozen monokarons, combine pairs until a great strain emerges.

Excellent clones can be found and maintained for years. Most people go that route because it tends to produce decent yields while you are looking. Isolation on agar is worthwhile but takes forever.


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Pastywhyte]
    #24208865 - 03/31/17 07:30 PM (3 years, 5 months ago)

one of these days I'll be able to afford a dissection micromanipulation scope and work with single spores

http://www.singerinstruments.com/solution/sporeplay/


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: bodhisatta]
    #24208866 - 03/31/17 07:32 PM (3 years, 5 months ago)



I want one of these too, for vodka and petri dishes


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Re: pour agar TEK [Re: bodhisatta]
    #24208882 - 03/31/17 07:40 PM (3 years, 5 months ago)

Good Lord, The toys never end. I thought my home made magnetic stirrer was cool.


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Offlinemushpunx
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Re: pour agar TEK [Re: Pastywhyte]
    #24209161 - 03/31/17 09:35 PM (3 years, 5 months ago)

Quote:

Pastywhyte said:
RR posted from ideal standpoints. Ideally it's even better to obtain 2 dozen monokarons, combine pairs until a great strain emerges.

Excellent clones can be found and maintained for years. Most people go that route because it tends to produce decent yields while you are looking. Isolation on agar is worthwhile but takes forever.




This is how I treat it. I mostly clone but I work on strain isolation in my free time. I really enjoy culture work so its fun for me



--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (03/31/17 09:45 PM)


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: mushpunx]
    #24209462 - 04/01/17 12:02 AM (3 years, 5 months ago)

It is true to get single strain isolates would be extremely more easily done with single spore germination and then mating. A micro manipulation scope could accomplish that


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Re: Bod's Comprehensive Agar TEK [Re: bodhisatta]
    #24210613 - 04/01/17 01:27 PM (3 years, 5 months ago)

I just successfully made my first batch of sterilized agar thanks to your excellent write up. Thanks for taking the time to share your knowledge  :awesomenod:


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OfflinemushboyM
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Re: Bod's Comprehensive Agar TEK [Re: Sporecupine]
    #24210620 - 04/01/17 01:30 PM (3 years, 5 months ago)

:thumbup: agar is fun. more fun than watching grains colonize:thumbup:

props on the name. please dont ruin it.:awedance:


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InvisiblebodhisattaM
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Re: Bod's Comprehensive Agar TEK [Re: Sporecupine]
    #24210625 - 04/01/17 01:31 PM (3 years, 5 months ago)

:cheers:

Its all about the propagation of our mushroom friends


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InvisibleGerms
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Re: Bod's Comprehensive Agar TEK [Re: bodhisatta]
    #24215822 - 04/03/17 06:16 PM (3 years, 5 months ago)


Thanks again man. Excited for this next step.


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InvisiblebodhisattaM
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Re: Bod's Comprehensive Agar TEK [Re: Germs]
    #24215965 - 04/03/17 07:16 PM (3 years, 5 months ago)

Nice


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Re: Bod's Comprehensive Agar TEK [Re: bodhisatta]
    #24225624 - 04/07/17 03:56 PM (3 years, 5 months ago)

Hey fellas.

  Do you feel like it's possible for water in a petri to leave stains where it ran without being contaminated? I've noticed very light, hardly visible stains from time to time that don't seem like a problem and don't seem to ride along when i transfer to new plates. I know pictures would help but I can't pull that off right now.


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InvisibleGerms
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Re: Bod's Comprehensive Agar TEK [Re: verum subsequentis]
    #24225666 - 04/07/17 04:30 PM (3 years, 5 months ago)

Could possibly be minerals in the water that are drying up, like water spots on dishes washed in tap water with high hardness. Like you said it's hard to tell without a picture though


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InvisiblebodhisattaM
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Re: Bod's Comprehensive Agar TEK [Re: Germs]
    #24225688 - 04/07/17 04:48 PM (3 years, 5 months ago)

Sounds bad but pictures :shrug:


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There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Re: Bod's Comprehensive Agar TEK [Re: bodhisatta]
    #24225884 - 04/07/17 06:34 PM (3 years, 5 months ago)

I'll try and get some pictures up soon. All my petris from that last round are sealed up and in my myco fridge. I like to keep them on hand as backups until the next transfers are Obviously healthy and wonderful. They are a bit tricky to photograph because when i take them out of the fridge they condensate pretty badly.

I'm pretty sure its the hard water issue though. I'll do my next batch with distilled and see if it changes anything.


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Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon ½ Pint Jars, Agar, Magnetic Stirrer, Petri Dish, Scales

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