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Offlinemushpunx
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Re: pour agar TEK [Re: Rickie]
    #24182340 - 03/22/17 07:30 AM (3 years, 5 months ago)

I just finally sat and read thru this fully, bravo man it really is a great write up. It was easy to read , entertaining etc

Most of this was stuff I already knew by now, but was able to get a better understanding of thru reading this
I learned stuff today


Actually- and this is embarrassing but I really like the little swipe videos. I prefer to go from print with a loop, but I do have some spore syringes, and it never occured to me to drop onto the plate before swiping- I always did this award motion dropping onto the loop and holding it in via water tension before swiping- its messy, and just not a very good way to do things :lol:


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: mushpunx]
    #24182446 - 03/22/17 09:49 AM (3 years, 5 months ago)

:rockon:


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Re: pour agar TEK [Re: bodhisatta]
    #24182588 - 03/22/17 10:55 AM (3 years, 5 months ago)

I finally got around to reading your Tek.
Lots of good info here wow, great explanations with pictures!!! I will add it to my signature later on today sometime. It should be a requirement for people to read when they first start out, in my opinion.


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: MadSeasonStudent]
    #24183349 - 03/22/17 03:39 PM (3 years, 5 months ago)

Haha, :rockon:


--------------------
police violence is no accident
ACAB
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Re: pour agar TEK [Re: bodhisatta]
    #24192245 - 03/25/17 05:06 PM (3 years, 5 months ago)



waitin


--------------------
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OfflineOrganic_Magic
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Re: pour agar TEK [Re: bodhisatta]
    #24192695 - 03/25/17 08:03 PM (3 years, 5 months ago)

Man this is so helpful, I always just fucked around with agar but after reading this you got me throwing stuff out of my fridge making space for slants. I found some monster boomers in galveston a few weeks ago but i didn't have any plates for a tissue clone and my new pc gasket hadn't got here yet so this week i went back, got a chunk of cow shit at the base of some dry as cubes, and tried to drop some mycelium from that onto a few plates. hopefully something happens because these caps were the size of CD's.


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:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




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Offlinemushpunx
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Re: pour agar TEK [Re: bodhisatta]
    #24192771 - 03/25/17 08:35 PM (3 years, 5 months ago)


Quote:

bodhisatta said:


waitin





You know after reading this I checked over a few lab water baths- I found a few used ones for real cheap , less than 40-50$ . If I ever get my spare room back I might get one- not just for agar but because it would be nice to have a proper one for my DMT extractions, that and a larger Seperatory funnel would rock


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: mushpunx]
    #24192838 - 03/25/17 09:02 PM (3 years, 5 months ago)

It would be easy to build one too.

Galveston island?


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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OfflineOrganic_Magic
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Re: pour agar TEK [Re: bodhisatta]
    #24192928 - 03/25/17 09:32 PM (3 years, 5 months ago)

yeah, I do swimming pool equipment repairs and this guy has about a 200 acre vacation ranch right off the beach with about 75 head of cattle. Been doing maintenance on the pool for over a year and never noticed any actives then one day last week I look out and see bright orange and i got giddy like a little girl lol. over the next 3 days i found 2-1/2 dry oz. Some truly MASSIVE texas coast cubensis. I need to upload the pics.


--------------------
:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




            click and learn.

Bod's simplified cultivation methods
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InvisiblebodhisattaM
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Re: pour agar TEK [Re: Organic_Magic]
    #24193074 - 03/25/17 10:28 PM (3 years, 5 months ago)

Wild print :begger:


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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OfflineOrganic_Magic
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Re: pour agar TEK [Re: bodhisatta]
    #24193115 - 03/25/17 10:47 PM (3 years, 5 months ago)

I'm pretty sure more will be popping up soon we got a heavy rain last night. I'll make sure to print one just for you bod.

:jackiechanofapproval:

Heres a couple of the big mofos i found. Im kicking myself for not printing them. :facepalm:





--------------------
:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




            click and learn.

Bod's simplified cultivation methods
Got side pins?


Edited by Organic_Magic (03/26/17 12:19 PM)


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Onlineverum subsequentis
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Re: pour agar TEK [Re: Organic_Magic]
    #24207891 - 03/31/17 01:14 PM (3 years, 5 months ago)

Great write up man.
 
  I really appreciate the members that take the time to experiment, learn and share. I almost never ever post anything (just read my brains out) but i can't seem to figure this out. hopefully you guys know the answer.
 
    Say my dog got too excited, unstacked his petris and transferred (3rd transfer from ms) before they were satisfactorily cooled. This resulted in unreasonable condensation. He's now asking me this question, "Is it possible for the liquid in the petri to run around and combine the genetics of each sector thereby making a pure isolated transfer impossible?"
 
      I don't know what to tell him. It seems that all the sectors could end up combining (anastamosis, I believe) or just temporarily muddling up and making this transfer basically ineffectual. It appears that he may be coincidentally creating a miny semi isollated lc on agar.
 
      Read the part about putting a cup of warm water on top to take care of it and I'll have him try. The problem is he aleady picked up a few and held them above his head to the light. This is when he saw water crawling over the sectors and got nervous.
       
              P.S. I think He's falling in Love with this Magical hobby


Edited by verum subsequentis (03/31/17 01:23 PM)


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis]
    #24207911 - 03/31/17 01:21 PM (3 years, 5 months ago)

Incompatibility is incompatible
And compatibility is compatible.

Compatible strains fuse regardless. And you cant force fusion of incompatibility

There's no fusion caused by your sloppiness(which gets better with practice)

There's no too much reason to do more than 2 or 3 transfers from MS all you're trying to do is get clean mycelium


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Onlineverum subsequentis
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Re: pour agar TEK [Re: bodhisatta]
    #24207922 - 03/31/17 01:26 PM (3 years, 5 months ago)

Ok, i understand some of that. but what of shooting for a kick ass pure isolate that He's read so much about.

thanks for the response, by the way.

And just so you know, He's probably a little bit more involved in all this than you would assume. He's not just going for clean myc. He's going for twelve isolates to throw in to rye and then mini trays of his desired sub (probably hpoo, straw, coffee, gypsum) to find the "stellar performer" and make a master slant of for the long haul.
    He'd gladly stop transfering now and begin the spawn run but believes there to be benifits in isolation.


Edited by verum subsequentis (03/31/17 01:39 PM)


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis]
    #24207969 - 03/31/17 01:48 PM (3 years, 5 months ago)

If you want a kickass isolate find 100 of them and throw out 99 after testing. Keep the good one

Also talking like it's not you is only annoying. It in no way protects you at all.

I would stop transfers now Grow it out then take a clone. Slant the clone and keep growing the clone


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Re: pour agar TEK [Re: bodhisatta] * 1
    #24208007 - 03/31/17 02:01 PM (3 years, 5 months ago)

I don't see how it could be all too annoying. i think it's funny. But I'll quit "annoying" you. Thanks


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis]
    #24208040 - 03/31/17 02:16 PM (3 years, 5 months ago)

Swim and my friend are useless. Offer no protection. And just make reading those replies cringy

For the most part it reminds us of 17 year olds still at mom's place because the research wasn't there to notice not a single other person talks like that here.

Im not personally annoyed. Im just saving you the hassle of getting flamed. Unfortunately theres Plenty of people who would love the opportunity to belittle and yell at someone for stupid stuff like that. I'd rather just mention in passing that it's not the way things go here so you can fit in quickly


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Re: pour agar TEK [Re: bodhisatta]
    #24208192 - 03/31/17 03:12 PM (3 years, 5 months ago)

Ok. Thanks for caring.
    I'm certainly not a 17 year old at mom's house. I just thought it was funny. But to clear this up, are your actually suggesting that 1 in 100 isolates will be amazing. I was thinking more like 1 in 20. I've been trying to decide between spawning now and continuing isolation. In my mind, basically, the farther out i isolate, the higher the probability of having a bunk or kick ass strain (meaning I'll get whatever the genetics i isolate have to offer). While the closer to ms i stay the higher the probability of an average performer (due to the combining of all compatible genetics, leaving me with a composite). Would you agree to that logic? It seems totally obvious.


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InvisiblebodhisattaM
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Re: pour agar TEK [Re: verum subsequentis]
    #24208390 - 03/31/17 04:22 PM (3 years, 5 months ago)

I wouldn't agree no.

The more you get to a single strain isolate the chances of bunk goes up but that kickass one may have been in a clone you could have gotten way easier a lot sooner

Ms to agar. Get clean culture, grow, clone, keep the clone.

Isolate work in spare time

1 in 20 or 1 in 100 are probably in the realistic realm if you go in blindly and have to test every one.

Clones are often times pretty close to isolates if not isolates right off the bat

The closer you stay to ms the better chances of that awesome performance one popping up for you to be able to clone


--------------------
police violence is no accident
ACAB
There's two kinds of people. Those who have had a bad interaction with cops and those who haven't got their turn


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Re: pour agar TEK [Re: bodhisatta]
    #24208506 - 03/31/17 05:06 PM (3 years, 5 months ago)

Alright, it seems that it's pretty easy to run into a million different opinions on the "best" way to do things around here. I've seen RR post time and again. MS-agar-isolate a handful-test all isolates and keep the best.
I really appreciate you taking the time to talk with me.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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