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Re: pour agar TEK [Re: PurePleasure] * 1
    #21922200 - 07/09/15 09:45 PM (8 years, 6 months ago)

Nice pics, good description :thumbup:

10-15 min is a little short of a PC run tho, for sure if your using grain soak water or the like I would up that.


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Re: pour agar TEK [Re: bodhisatta] * 2
    #21922353 - 07/09/15 10:10 PM (8 years, 6 months ago)

I usually go for 20 most of the time and 45 for grain water. Never really had issues but I don't work with yeasts or peptone. Usually just work with whats laying around the house :tongue:

I have had grain water LC go bacterial if I don't run a full 45 min.


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Re: pour agar TEK [Re: bodhisatta] * 3
    #21922367 - 07/09/15 10:15 PM (8 years, 6 months ago)

Maybe its the elevation I'm at but when grain water LC shows bacteria on the test plate, I don't argue with it.


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Re: pour agar TEK [Re: invitro] * 1
    #21922511 - 07/09/15 11:02 PM (8 years, 6 months ago)

Back on topic I like the idea of the bath to bring down to temp. I am a cheap fucker and prefer to by tetracycline from the pet store than gentamiacin. The bath would speed up the wait for the agar to cool enough to add the tetra.


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Re: pour agar TEK [Re: bodhisatta] * 1
    #24208786 - 03/31/17 04:59 PM (6 years, 9 months ago)

RR posted from ideal standpoints. Ideally it's even better to obtain 2 dozen monokarons, combine pairs until a great strain emerges.

Excellent clones can be found and maintained for years. Most people go that route because it tends to produce decent yields while you are looking. Isolation on agar is worthwhile but takes forever.


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Re: BOD's Comprehensive Agar TEK [Re: bodhisatta]
    #26167508 - 09/03/19 06:49 PM (4 years, 4 months ago)

I like pour agar alright, I don’t like the cost of shipping to Canada. I did hate pouring in a SAB though now that I have a hood I still mostly use no pour.


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