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Jsneeb
Drifter


Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
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Pan Cyans Attempt 3 Contam every time
#21918057 - 07/09/15 04:53 AM (8 years, 6 months ago) |
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Ok, Woke up today to check on my latest tray, spawned on Monday.
Opened it up. Looks exactly like it did on Monday when spawned. Smells a bit like wine, yeasty smell.
Bacteria.
What the shit?
This is the third time this has happened. The first time I spawned to poo/straw and thought the straw was the source. Next time I spawned 1/1 to maunure no straw.
This time I spawned to no bulk at all. Just into 1 quart ziplock round trays.
WHAT THE SHIT?
In the jars before I Spawn everything looks healthy. Growth is fast, no spots in the grain where it doesnt take off. If I shake it comes back within 24 hours.
Here is a hiustory of this grow attempt.
Recived Print on foil.
Print put on Agar, 4 dishes Incubated 10 days.
All prints looked great except one with trich. Thrown away.
Best dish chosen to transfer to 4 more dishes.
3 days all 4 new dishes show good growth.
3 dishes cut in half and half an agar wedge placed per grain jar. ( 6 total )
One dish used to make LI.
Agar placed to half full jars of WBS with a handful of manure ( worked awesome for my Cube grows)
Close jar, shake.
wait 5 days or 20 % colonization. Shake again.
Spawn 1 to 2 days after 100 %.
All my work is done in a SAB. Its not the best but it's been ok for my cube grows. I spay it down with alohol/soap solution and wash my hands with bleach solution. I have no gloves or sleves for my box. Again, I has worked for cube grows and my agar has only shown contams less than 2 times out of 20 dishes though there.
-------------------- Read my trip report Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter? http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pan Cyans Attempt 3 Contam every time [Re: Jsneeb]
#21918088 - 07/09/15 05:19 AM (8 years, 6 months ago) |
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post pics of ur plates. and pics of ur jars at 100%. bacteria should be easy to spot, mold is harder.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Pan Cyans Attempt 3 Contam every time [Re: blindingleaf]
#21918115 - 07/09/15 05:38 AM (8 years, 6 months ago) |
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I find bacteria hides better under the mycelia, compared to moulds. After being introduced to new air the moulds tend to sporelate quickly, and expand even quicker. Bacteria just sorta creeps.. So if its under the mycelia.. Your basically playing russian roulette with your grains.
I usually have to sandwich my agar using a "hot pour" to separate the mycelia from bacteria.
-------------------- Giving is all we have, for we're just grateful to be alive

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Jsneeb
Drifter


Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
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How do you do this " hot pour "
I'll post pics in a bit.
-------------------- Read my trip report Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter? http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Pan Cyans Attempt 3 Contam every time [Re: Jsneeb]
#21920728 - 07/09/15 04:21 PM (8 years, 6 months ago) |
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Simply pour hot agar over the contaminated plate, the increase in tempature seems to slow most molds and bacterial growth, long enough for the mycelia to break through and take a foot-hold. Which only takes 24-48hrs. As soon as the mycelia breaks through shave the top of the dish and transfer a piece of myclia to a new dish without taking any of the agar from the contaminated dish. (you only want the mycelia)
If necessary, repeat on the new dish until you get a clean culture.
-------------------- Giving is all we have, for we're just grateful to be alive

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mycomaniac1402
Myco Maniac :-)



Registered: 01/25/15
Posts: 257
Last seen: 2 months, 19 days
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Re: Pan Cyans Attempt 3 Contam every time [Re: Jsneeb]
#21920730 - 07/09/15 04:21 PM (8 years, 6 months ago) |
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It could be your grain prep... Most likely or its the agar ? Maybe try going simple... I have never failed using modified pf cakes as spawn to h manure and straw. Very simple and contam resistant. If you agar is causing you troubles move on to something you are sure you can do , get some fruits then play around with perfecting your agar skills. Ive never been big in agar. Just never saw a need. To each their own though
But it could be something totally different and not the agar, as grain can be tricky for some. Have you had success with grain working with Cubes?
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nooneman


Registered: 04/24/09
Posts: 14,561
Loc: Utah
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Re: Pan Cyans Attempt 3 Contam every time [Re: Jsneeb]
#21920766 - 07/09/15 04:35 PM (8 years, 6 months ago) |
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Your problem is that you're using WBS. Never use WBS.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Pan Cyans Attempt 3 Contam every time [Re: nooneman]
#21920774 - 07/09/15 04:36 PM (8 years, 6 months ago) |
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Wbs is not the culprit, although Wbs is a poor choice of grain.. IMO WHOLE OATSSS all dayy
-------------------- Giving is all we have, for we're just grateful to be alive

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nooneman


Registered: 04/24/09
Posts: 14,561
Loc: Utah
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Quote:
Fantastic Mr. Fox said:

Wbs is not the culprit, although Wbs is a poor choice of grain.. IMO WHOLE OATSSS all dayy
WBS is definitely the culprit. It has a way higher contam rate even with cubes, and pan cyan shouldn't be grown on it at all. It's terrible and no one should ever use it.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Pan Cyans Attempt 3 Contam every time [Re: nooneman]
#21920806 - 07/09/15 04:47 PM (8 years, 6 months ago) |
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.........
 Your hilarious brav,
Ive used wbs in the past with no problem. Maybe something was wrong with your preparation/sterilization technique. No offense, just know plenty tht have used wbs & will continue to use wbs because of its low end price & success rate;Granted their preparation/sterilization technique was on par
-------------------- Giving is all we have, for we're just grateful to be alive

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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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most wbs mixes has plenty of milo/millet which is awesome for both cubes and pans!
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Pan Cyans Attempt 3 Contam every time [Re: spacechildo]
#21920882 - 07/09/15 05:05 PM (8 years, 6 months ago) |
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-------------------- Giving is all we have, for we're just grateful to be alive

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Jsneeb
Drifter


Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
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To answer the questions in this thread.
I have done 3 grows using WBS with cubes.
Success every time. My sterile technique was identical each time and with my Cyans.
My prep is as follows.
Wash WBS, Remove all "floaters"
Fill large pot and leave them submerged in warm conditions for 24 hours. This is known to germinate endospored making them succeptable to death by high temps.
This also soaks the grain.
Simmer on medium heat / low boil for 30 minutes. This soaks the grain to capacity and germinates the rest of the bacteria and endospores.
Leave in strainer for 5-10 minytes to drip dry.
Fill jars 60-75% full.
PC at 15 PSI for a minimum of 90 minutes. Cover Pressure cooker and allow to cool.
My cooker will retain heat and pressure for another 40 minutes after I remove it from heat.
My cubes have had only one of 15 jars fail.
6 were inoculated with MS. Great success, On second flush. 2 Were agar to LI. Current fruiting. 4 were agar to grain. Currently colonizing. 2 were grain to grain experimentation from the first clean batch. Currently in fridge.
So WBS clearly works if you follow the Tek and PC the full time + more.
How do bacteria get in? Is it the spawn or spawning procedure? Like I said my jars look perfect before I spawn them.
-------------------- Read my trip report Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter? http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Pan Cyans Attempt 3 Contam every time [Re: nooneman]
#21921085 - 07/09/15 05:51 PM (8 years, 6 months ago) |
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Quote:
nooneman said: Your problem is that you're using WBS. Never use WBS.
Piss off with that. WBS works just fine if you do it right. I've used it for the better part of a decade.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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mycomaniac1402
Myco Maniac :-)



Registered: 01/25/15
Posts: 257
Last seen: 2 months, 19 days
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Re: Pan Cyans Attempt 3 Contam every time [Re: 36fuckin5]
#21921143 - 07/09/15 06:07 PM (8 years, 6 months ago) |
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Okay the other thing I can think of is Pan mycellium smells alot different in a citrus kinda way. After re reading your post do you think this could be what you smelled. Was it growing fine and just smelled odd? It smells like lime to me... Not earthy as cubes its definitely different. Im trying to figure out if you experience hindered growth , did you give it enough time to even see if it was hindered or just tossed it after smelling something off. Or was it nasty rotton smell like apple sweet smell... Even if I do smell a hint of something odd I always see if it will go ahead and acclimate to the presence of bacteria to see if it'll pull through , it doesn't happen often but when it did it was always fine. But im thinking your smelling a different mycellium and thinking its a bacteria inf. Possible?
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Pan Cyans Attempt 3 Contam every time [Re: mycomaniac1402]
#21921157 - 07/09/15 06:09 PM (8 years, 6 months ago) |
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Smells fishy to me..
-------------------- Giving is all we have, for we're just grateful to be alive

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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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when i first grew them, pasty told me it would smell like cucumbers, and thats all i smell when i smell them now
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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