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Offlineshellzenone
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Re: MEA and PDA agar questions [Re: shroomyaxn]
    #21923704 - 07/10/15 09:10 AM (8 years, 6 months ago)

I'm curious to know, how long do you guys wait after pc-ing  to pour the agar?


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InvisibleMad Season
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Re: MEA and PDA agar questions [Re: shellzenone]
    #21923943 - 07/10/15 10:31 AM (8 years, 6 months ago)

Put the media bottle in a dish of water at 60ish C. Once the water in the dish gets to 47, you can assume the agar media is around 47 too. Pour at that moment.


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Edited by Mad Season (07/10/15 10:32 AM)


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OfflinefirstTIMER420
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Re: MEA and PDA agar questions [Re: shroomyaxn]
    #21926237 - 07/10/15 09:20 PM (8 years, 6 months ago)

Quote:

shroomyaxn said:
Quote:

NumeroEno said:
I also put down fresh lysol wipes as my work surface.




Me too.  Also, instead of working directly on top of a towel, i created an elevated rack to work on. 
I used those rubber coated wire shelves.   


I took two wire shelf pieces, cut them to size and zip tied them together.  The shelf lip at each end creates an elevated rack of sorts.

Everything placed on the rack inside the SAB is not coming into contact with that much surface area at all.






I put down a towel and place the open SAB top down on top of the towel(all sprayed of course), but I might replace it with this method, or a grid pattern created out of some sort of wire, nice engineering man


Also, I am going to start the agar tomorrow, the thing is, I still don't have a tek to follow, Im just going to use the pre mixed PDA that I have, and follow the LGM(RR) vid in terms of agar, then just kinda make up the rest from info on this post.  That is, unless someone has a good link to an agar tek for MEA...

also, using the pre made PDA powder is basically heat/mix with water, PC, then pour?


ALso, numero or anyone else, what temp should I heat the agar mixture too when heating in the saucepan?


wouldn't the plates being wrapped up interfere with the GE?


Edited by firstTIMER420 (07/10/15 09:34 PM)


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Offlineshroomyaxn
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Re: MEA and PDA agar questions [Re: firstTIMER420]
    #21930920 - 07/11/15 09:23 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
I put down a towel and place the open SAB top down on top of the towel(all sprayed of course), but I might replace it with this method, or a grid pattern created out of some sort of wire, nice engineering man





Thanks man.

Quote:

firstTIMER420 said:
Also, I am going to start the agar tomorrow, the thing is, I still don't have a tek to follow, Im just going to use the pre mixed PDA that I have, and follow the LGM(RR) vid in terms of agar, then just kinda make up the rest from info on this post.  That is, unless someone has a good link to an agar tek for MEA...





I have pre-mixed LME from a sponsor that i mix with agar inside the flask. 
10 grams light malt extract
10 grams Agar
500 ml water

500ml can make 20 petri dishes. 

Quote:

firstTIMER420 said:
wouldn't the plates being wrapped up interfere with the GE?





I use parafilm.  It is gas permeable.


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InvisibleNumeroEno
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Re: MEA and PDA agar questions [Re: shroomyaxn]
    #21930996 - 07/11/15 09:39 PM (8 years, 6 months ago)

Yup. 10 grams LME, 10 grams agar powder, and 500ml of water is a great agar recipe, and it will pour 20 100mm plates exactly :thumbup:


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OfflinefirstTIMER420
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Re: MEA and PDA agar questions [Re: NumeroEno]
    #21931030 - 07/11/15 09:49 PM (8 years, 6 months ago)

I mix those in a saucepan while heating right?  But, how much do I heat up the mixture, till its boiling or just a simmer?


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InvisibleNumeroEno
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Re: MEA and PDA agar questions [Re: firstTIMER420]
    #21931048 - 07/11/15 09:55 PM (8 years, 6 months ago)

Add your agar powder while the water is cold. Trust me.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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OfflineICEMANOO9
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Re: MEA and PDA agar questions [Re: NumeroEno]
    #21931095 - 07/11/15 10:04 PM (8 years, 6 months ago)

It may not be exactly what you need but hope it helps.

http://www.shroomery.org/forums/showflat.php/Number/20111637/fpart/all/vc/1


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OfflinefirstTIMER420
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Re: MEA and PDA agar questions [Re: NumeroEno]
    #21931107 - 07/11/15 10:06 PM (8 years, 6 months ago)

so LME and water first, then add agar, then heat up while mixing.


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Offlineshroomyaxn
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Re: MEA and PDA agar questions [Re: firstTIMER420]
    #21931150 - 07/11/15 10:15 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
so LME and water first, then add agar, then heat up while mixing.




No need to heat up.  I add dry ingredients to flask, add water then mix, wait, then mix again.


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OfflinefirstTIMER420
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Re: MEA and PDA agar questions [Re: shroomyaxn]
    #21931184 - 07/11/15 10:25 PM (8 years, 6 months ago)

gotcha, anyone else not heat? most teks ive seen say to heat I think to sterilize.


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Offlineshroomyaxn
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Re: MEA and PDA agar questions [Re: firstTIMER420]
    #21931200 - 07/11/15 10:31 PM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
gotcha, anyone else not heat? most teks ive seen say to heat I think to sterilize.




The sterilizing comes in the PC, after the mixing.


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OfflineICEMANOO9
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Re: MEA and PDA agar questions [Re: shroomyaxn]
    #21931229 - 07/11/15 10:40 PM (8 years, 6 months ago)

:whathesaid:


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OfflinefirstTIMER420
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Re: MEA and PDA agar questions [Re: shroomyaxn]
    #21931314 - 07/11/15 11:10 PM (8 years, 6 months ago)

im going to try giving it some light heat I guess, as I said most teks call for it.


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InvisibleNumeroEno
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Re: MEA and PDA agar questions [Re: firstTIMER420]
    #21931819 - 07/12/15 01:39 AM (8 years, 6 months ago)

It works best if you add the ingredients to cold water, and then turn the heat on and stir constantly until the agar is dissolved.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Offlinehamloaf
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Re: MEA and PDA agar questions [Re: firstTIMER420]
    #21932191 - 07/12/15 05:56 AM (8 years, 6 months ago)

Quote:

firstTIMER420 said:
im going to try giving it some light heat I guess, as I said most teks call for it.



The reason heat is generally applied during the mixing of the ingredients is to aid in the dissolving of the ingredients themselves.  The purpose of the pressure cooker is to sterilize the media.


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OfflinefirstTIMER420
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Re: MEA and PDA agar questions [Re: hamloaf]
    #21932477 - 07/12/15 08:25 AM (8 years, 6 months ago)

gotcha, thanks guys.  All I need now is a bottle to have all the ingredients in.

and  btw ham, I just looked at the cube monotub link in your sig...

  :youthemandawg:  :highfive: THEM PINS


have you written up the tek yet for the "hybrid" design of the tubs?

id also be inclined to see the Spawn to Sub ratios used in producing those tubs in that tek as well.. for information purposes, but also to rub it in peoples faces :lol:


Edited by firstTIMER420 (07/12/15 08:50 AM)


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