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OfflineMysticPop
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True hepa purifier vs flow hood
    #21916610 - 07/08/15 07:43 PM (8 years, 6 months ago)

Hi all

Had a basic question. Can we use a true hepa air purifier in place of laminar flow hood.

Cheers
Mystic


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InvisibleCH HELL
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Re: True hepa purifier vs flow hood [Re: MysticPop]
    #21916691 - 07/08/15 08:02 PM (8 years, 6 months ago)

NO.
Flow hoods work because of air currents not allowing contaminates to drop giving you a small area to work in front of it.

CH


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OfflineShroominMe
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Re: True hepa purifier vs flow hood [Re: MysticPop]
    #21916707 - 07/08/15 08:05 PM (8 years, 6 months ago)

A flow is preferable for most mycology tasks.  That's why professionals use them.  They cost a LOT $1,000+.

Yes, I did say MOST, thank you.

An HEPA air purifier helps a lot but you'll still have to practice sterile technique.  You can get a good one for $100 give or take.  I live in a high contam environment so an HEPA AP is a must for me.


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OfflineMysticPop
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Re: True hepa purifier vs flow hood [Re: ShroominMe]
    #21916970 - 07/08/15 08:52 PM (8 years, 6 months ago)

So which HEPA AP do you use? Do you have a link ?


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OfflineShroominMe
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Re: True hepa purifier vs flow hood [Re: MysticPop]
    #21917199 - 07/08/15 09:51 PM (8 years, 6 months ago)

The one I bought 5 years ago is no longer available.  Prices seem to have more than doubled since then, unfortunately.  What happened to competition?

I'd do a search via Amazon.  Make sure it's true HEPA and not "HEPA type"!!!

Unfortunately you're not going to be able to get away with a cheapo.  Plan on $150+ and if you can get one for less be happy. 

An investment in this hobby and also, equally important, your general health.


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Invisibleazur
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Re: True hepa purifier vs flow hood [Re: ShroominMe]
    #21917222 - 07/08/15 09:57 PM (8 years, 6 months ago)


See the difference?


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InvisibleCH HELL
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Re: True hepa purifier vs flow hood [Re: azur]
    #21918984 - 07/09/15 10:26 AM (8 years, 6 months ago)

Building a flow hood isn't that expensive.

A hepa purifier is about worthless to a mycologist.  Yes it is nice for your health to get rid of some nasty shit floating in the air but you could run one constantly in a small room and never have the air clean enough to do agar work.  A Hepa purifier is not an investment into this hobby.  If you are just looking for a cheap investment build a SAB,  works better and it is cheaper.

CH


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InvisiblebodhisattaMDiscordReddit
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Re: True hepa purifier vs flow hood [Re: CH HELL]
    #21919441 - 07/09/15 11:20 AM (8 years, 6 months ago)

:whathesaid:
It's all about techniques and skill.


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Offlineshellzenone
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Re: True hepa purifier vs flow hood [Re: bodhisatta]
    #21919478 - 07/09/15 11:28 AM (8 years, 6 months ago)

If you do decide to use a SAB, don't run the air purifier while you're using the SAB. You should have relatively good success by eliminating all air currents and using sterile technique. Make sure you spray the inside of the SAB with lysol or a solution of water and Clorox. Keep the insides of the SAB moist with the sanitizer of your choice. :thumbup:


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OfflineMysticPop
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Re: True hepa purifier vs flow hood [Re: shellzenone]
    #21919514 - 07/09/15 11:34 AM (8 years, 6 months ago)

Hi

I already have a SAB. See picture below. However Its a pain in the ass to work with a SAB when the top is translucent. Also it becomes difficult to heat the needle and the knife inside the SAB as i mist mine with 70% ISO. Am i missing any sterile technique here? I just want to improve the rate of success by using better equipment. I will try to build a laminar flow hood instead of investing in hepa purifier.





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Invisible36fuckin5
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Re: True hepa purifier vs flow hood [Re: MysticPop]
    #21919633 - 07/09/15 11:54 AM (8 years, 6 months ago)

Remove the gloves. Don't flame things inside your box, especially when it's wet with alcohol. That's just fucking dumb. Don't wet it down with iso, use bleach water or water with a little soap.

Go read.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlinespacechildo
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Re: True hepa purifier vs flow hood [Re: shellzenone]
    #21919674 - 07/09/15 12:01 PM (8 years, 6 months ago)

Quote:

shellzenone said:
Make sure you spray the inside of the SAB with lysol or a solution of water and Clorox. Keep the insides of the SAB moist with the sanitizer of your choice. :thumbup:




why waste money on lysol or irritate your nose and mouth with clorox?

I use 1-2 drops of dish soap and a pint of water every 2-3 weeks :shrug:


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OfflineMysticPop
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Re: True hepa purifier vs flow hood [Re: spacechildo]
    #21919714 - 07/09/15 12:09 PM (8 years, 6 months ago)

Those are not gloves. those are tyvek sleeves. I use disposable gloves doused in 70% alcohol. Also i donot flame it inside the SAB when it is misted with 70% ISO and i have to flame outside the SAB.


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Offlineshellzenone
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Re: True hepa purifier vs flow hood [Re: spacechildo]
    #21920131 - 07/09/15 02:05 PM (8 years, 6 months ago)

Interesting... This is the first time I've read about this. I'll have to try this because it does irritate me even while wearing a dust mask.


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InvisiblebodhisattaMDiscordReddit
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Re: True hepa purifier vs flow hood [Re: shellzenone]
    #21920139 - 07/09/15 02:08 PM (8 years, 6 months ago)

If your sab is too small or hard to work in build a bigger one.... I really can't believe people dislike using a sab because its too small when they built it too small for themselves. That's dumb.


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OfflineMysticPop
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Re: True hepa purifier vs flow hood [Re: bodhisatta]
    #21920357 - 07/09/15 03:06 PM (8 years, 6 months ago)

Dude the issue is not that it is small..Its actually pretty big..155Qt.. The issue is visibility and working through the holes. If one has Laminar Flow then we can just work in open air in an enclosed area with free movements. As i said...I am not against SAB,,i am just looking for more convenience with freedom of movement.

If i build my Laminar Flow Hood.. i might soon be selling/trading my SAB and trust me its pretty dope!!


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Invisibleazur
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Re: True hepa purifier vs flow hood [Re: MysticPop]
    #21920391 - 07/09/15 03:11 PM (8 years, 6 months ago)

Laminar flow ftw.
My sab was big as shit. But compared to a flow hoof, it sucked. My hood is 30"x48"  I can bareback a hooker and safely do agar work at the same time.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblebodhisattaMDiscordReddit
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Re: True hepa purifier vs flow hood [Re: azur]
    #21920500 - 07/09/15 03:33 PM (8 years, 6 months ago)

even at work I opt not to use a FH, the extra upkeep of having to keep a lab space extra tidy, having to let it run before you use it, it eventually needs a replacement filter, and how not-discrete it is for home use.

Quote:

MudaFuka said:
I hung painters plastic around my work area to cut down on drafts.

I use that large SAB when working on things like G2G where I need more head space.

When I work with agar I put my small SAB inside of it.


Edit: Wow this thread is old. I thought frank was back around for a second there.:sad: 




even without the drop cloth a sab that size is just as effective as using a 100q tub. you can custom make a SAB that's tits as fuck for 20 dollars.

why work with 99.99% if you don't have the clean room to go along with it. it seems like 9:10 people on these forums with a laminar flow cabinet have it in a completely unsuitable room.


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Invisibleazur
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Re: True hepa purifier vs flow hood [Re: bodhisatta]
    #21920524 - 07/09/15 03:36 PM (8 years, 6 months ago)

"why work with 99.99% if you don't have the clean room to go along with it. it seems like 9:10 people on these forums with a laminar flow cabinet have it in a completely unsuitable room."

Not me


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblebodhisattaMDiscordReddit
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Re: True hepa purifier vs flow hood [Re: azur]
    #21920537 - 07/09/15 03:37 PM (8 years, 6 months ago)

yea well you went the extra mile and did that setup for yourself, who else has that, like three people?


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OfflineMysticPop
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Re: True hepa purifier vs flow hood [Re: bodhisatta]
    #21920541 - 07/09/15 03:39 PM (8 years, 6 months ago)

In the images above, i see that you have cut the plastic sheet from the cover of the SAB on top. Is that okay? I have not cut the top cover of the SAB and hence it is difficult to see. I was trying to reduce the contaminants by keeping the SAB sealed while working in it.


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Re: True hepa purifier vs flow hood [Re: azur]
    #21921449 - 07/09/15 07:19 PM (8 years, 6 months ago)

Quote:

azur said:
Laminar flow ftw.
My sab was big as shit. But compared to a flow hoof, it sucked. My hood is 30"x48"  I can bareback a hooker and safely do agar work at the same time.



its restrictive for sure. even with a large one. not to say it doesn't work. it slows things down is all. when you need to maximize efficiency, larger work space is a must. course i gave away my flowhood and replaced it with a 30$ hepa unit. :cool:

the workspace is almost triple the volume of my SAB and the SAB i have is fairly large.


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Offlinesauroman1
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Re: True hepa purifier vs flow hood [Re: azur]
    #26915744 - 09/03/20 01:52 PM (3 years, 4 months ago)

Quote:

azur said:

See the difference?




So if I use air purifier like BAP412 with hood box it will still stir up air? How exactly laminar air flow is produced?

Looks like biggest cost comes from HEPA or even better filtration, since true HEPA filters are costly to produce. Well they also need high power centrifugal fans to push trough tiny pores, 80W ain't enough?

Anybody here used non woven filters?


Edited by sauroman1 (09/03/20 01:56 PM)


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Offlinekookookook
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Re: True hepa purifier vs flow hood [Re: ShroominMe]
    #28232763 - 03/16/23 07:46 PM (10 months, 8 days ago)

Costco has HEPA air filters that go on sale now and then for $100.


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