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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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how to inoculate agar dishes
#21916393 - 07/08/15 07:00 PM (8 years, 6 months ago) |
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Hello... Quick question...I followed pasty's agar tek, and have the pda jars cooling right now in the pc...my question is, I didn't c what the best way to inoculate the plates would b?
I have prints, and one homemade spore syringe. Can I just simply scrape a few spores in there and close it up?
Thank you all so much! I really do LOVE this community
Ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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DensePlacebo
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21916404 - 07/08/15 07:02 PM (8 years, 6 months ago) |
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Yup. It's best done ina GB or SAB. But you can use the syringe or a little scraping of a print. I do believe the syringe is the better way to go tho, you spread less spores out which is what you want.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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mushpunx
Fungus Punk



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Re: how to inoculate agar dishes [Re: DensePlacebo] 1
#21916513 - 07/08/15 07:23 PM (8 years, 6 months ago) |
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A drop from a spore syringe will spread around on the agar and it can kind of make it hard to pick out clean/healthy growth sometimes.
If you use the syringe squirt a drop on a loop or a sterile cotton swab first.
To inoculate from the print, flame your loop, cool in a blank plate and swipe the print.. the agar will help pick up spores... then swipe the reciving plate. Swiping is good, youll have an idea of where to expect germination
--------------------
 Amateur Mycologists United AMU Q&A
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
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Re: how to inoculate agar dishes [Re: mushpunx]
#21916580 - 07/08/15 07:35 PM (8 years, 6 months ago) |
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Thank you guys so much...what exactly does "cool in a blank plate" mean?
Thank you, ganjaburger
--------------------
Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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SizlChest
Padawan



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Re: how to inoculate agar dishes [Re: Ganjaburger] 1
#21916619 - 07/08/15 07:47 PM (8 years, 6 months ago) |
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Means after flaming the loop, plunge the hot metal into a section of agar off to the side of the plate. This cools the tool so you can use it without damaging the spores. It also leaves melted agar on the loop, so the spores stick to the agar coating it.
Buy RR's Let's Grow Mushrooms videos. Very good investment to make, I still watch them from time to time.
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
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Re: how to inoculate agar dishes [Re: SizlChest]
#21916781 - 07/08/15 08:21 PM (8 years, 6 months ago) |
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Yes... the videos are definitely a good investment. They are a great reference tool. Though I have been into the hobby for a little while, I watch a vid(s) to freshen up or look up some info quite often.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
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Alright, so I made the pda mixture, pc'd, then let cool overnight...when I got them out, the bottom half solidified, top half was still liquid...what gives..I measured out the formula Damn near to the mg on my digital scale. Cooked on low. Added food color, also I can see that any sediment (I assume potato flakes that didn't fully dissolve) where all contained in the solid(bottom) section. I put them in fridge, and they are solidified all the way though, are they still good to use? Even tho I can c that all the sediment is in the bottom half, top half completely clear, besides food coloring.
Ty, I understand there is allot of questions, and I really appreciate the help!!!! :-)
Ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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Grey
⇜ ✯ ⇝



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Posts: 6,223
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21919979 - 07/09/15 01:14 PM (8 years, 6 months ago) |
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Sediment is fine. Mine look that way when I use instant potato flakes.
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SublimeLBC
dab master



Registered: 10/22/04
Posts: 618
Loc: inside your mother
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Re: how to inoculate agar dishes [Re: Grey]
#21919991 - 07/09/15 01:22 PM (8 years, 6 months ago) |
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--------------------
Edited by SublimeLBC (07/09/15 01:23 PM)
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Mr.GuessWork
Stranger

Registered: 03/30/13
Posts: 4,563
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Re: how to inoculate agar dishes [Re: Grey]
#21920011 - 07/09/15 01:28 PM (8 years, 6 months ago) |
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You're trying to isolate mycelium I take it? You want to do what's called a streak plate. The basic idea is to spread everything out on the surface of the agar so individual colony forming organisms are far apart before they start growing. That way when stuff has grown for a bit it won't all be jumbled together, and you can cut the sections you want out of the agar. Here's a video for the 4 streak method:
You probably don't need to do 4 streaks (2 or three might work fine). Make sure you re-sterilize your loop after each streak.
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DensePlacebo
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Re: how to inoculate agar dishes [Re: Mr.GuessWork]
#21920879 - 07/09/15 05:04 PM (8 years, 6 months ago) |
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IME pastys agar tek gave me realm watery agar. I still use that tek to this day but I use double the agar he calls for. I don't have sediment and I don't have lots of condensation.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 6 hours
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Re: how to inoculate agar dishes [Re: DensePlacebo]
#21921551 - 07/09/15 07:44 PM (8 years, 6 months ago) |
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If you put a folded piece of paper towel between the lid and foil it helps alleviate condensation and/or steam getting into the container.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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20g/L of agar-agar should give you appropriate consitency. 15g/L is most lab grade. if you buy agar from the store make sure it doesn't have added ingredients or sugar otherwise you're not adding as much as you think you are.
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
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Re: how to inoculate agar dishes [Re: bodhisatta]
#21922155 - 07/09/15 09:40 PM (8 years, 6 months ago) |
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Thank u guys so much for all the speedy answers...Ok..so feel like the more I learn, the more questions I think of..lol. but I have a pe swab...do I just streak it the same way I would if using an inoculation loop? Also, I've only worked with grain in all of my past grows... Recently I had a bunch of brf/verm given to me...I was obviously familiar with pf-tek...but never tried it...when I went to look up ratios of Verm/brf/h2o....I read a few different answers...so, what ratio do u guys use? I really want to try and "perfect"every technique...and recently ran into some extra funds so I can buy whatever is needed. Also, how long do ur guys brf jars take to show initial growth? I'm on day 4/5 with no noticeable growth...starting to get worried..sorry for the long one paragraph form..lol. 2mg clonazolam, &2mg alprazolam, and a couple beers..my grammar/writing skills go right out the window...lol
Thank you guys so much for all the help....I don't understand how Fuckin Facebook and instagram are so popular...the shroomery is a million x cooler
-Ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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DensePlacebo
Stranger



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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21922388 - 07/09/15 10:20 PM (8 years, 6 months ago) |
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I believe the streak is the same but wait for one of them others to clear that up. I use 1 part water 1 part flour mix that really good and add 2 parts verm I also add in a pinch or two of gypsum but there are a few different additives. I see growth in about a week so id say your fine still.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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Re: how to inoculate agar dishes [Re: DensePlacebo]
#21922471 - 07/09/15 10:52 PM (8 years, 6 months ago) |
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I've heard of adding gypsum...what is it, and y add it?
Thx ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,064
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21922565 - 07/09/15 11:21 PM (8 years, 6 months ago) |
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Quote:
Ganjaburger said: Hello... Quick question...I followed pasty's agar tek, and have the pda jars cooling right now in the pc...my question is, I didn't c what the best way to inoculate the plates would b?
I have prints, and one homemade spore syringe. Can I just simply scrape a few spores in there and close it up?
Thank you all so much! I really do LOVE this community
Ganjaburger
Use your own prints. Read the tiger drop tek in my signature. If you use pre-sterilized cotton swabs you won't even need to flame. If the print was taken with any sort of care you should be able to get clean growth on your initial inoculation and then you can drop the whole agar plate into a grain jar.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Jakeoncid419
Follow me.....


Registered: 06/26/18
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#25293010 - 06/26/18 12:31 PM (5 years, 7 months ago) |
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WhAt do u think of pre fabed agar dishes? Recommend vendor? I need to isolate cope stain for grain inoculation. Still rather new to agar and never made my own
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: how to inoculate agar dishes [Re: Jakeoncid419]
#25293104 - 06/26/18 01:45 PM (5 years, 7 months ago) |
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Premade plates are shit. Make your own plates. Also this is a two year old thread.
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Jakeoncid419
Follow me.....


Registered: 06/26/18
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Re: how to inoculate agar dishes [Re: bodhisatta]
#25294489 - 06/27/18 09:01 AM (5 years, 7 months ago) |
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Oh I didn’t even look at dates, does it matter that it’s a old thread? Sry for my ignorance new member although I’ve been just reading for years lol malt extract good route for copes ya think?
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: how to inoculate agar dishes [Re: Jakeoncid419]
#25294510 - 06/27/18 09:12 AM (5 years, 7 months ago) |
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Quote:
Jakeoncid419 said: Oh I didn’t even look at dates, does it matter that it’s a old thread? Sry for my ignorance new member although I’ve been just reading for years lol malt extract good route for copes ya think?
Yes, Malt Extract Agar is a great route to take...and always check the thread dates
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Jakeoncid419
Follow me.....


Registered: 06/26/18
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Re: how to inoculate agar dishes [Re: Tormato]
#25294753 - 06/27/18 11:04 AM (5 years, 7 months ago) |
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But I can’t start my own posts yet so if there are no recent relevant threads idk what to do and I can’t make new posts until I make so many posts lol it’s a paradox
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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Sivarted
Stranger
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Re: how to inoculate agar dishes [Re: Jakeoncid419]
#25294761 - 06/27/18 11:08 AM (5 years, 7 months ago) |
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Quote:
Jakeoncid419 said: But I can’t start my own posts yet so if there are no recent relevant threads idk what to do and I can’t make new posts until I make so many posts lol it’s a paradox
There are a ton of threads about premade petris.
Use the search engine.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: how to inoculate agar dishes [Re: Jakeoncid419]
#25294785 - 06/27/18 11:18 AM (5 years, 7 months ago) |
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Quote:
Jakeoncid419 said: But I can’t start my own posts yet so if there are no recent relevant threads idk what to do and I can’t make new posts until I make so many posts lol it’s a paradox
Yes you can make posts lol. just press post. No paradox just you very confused.
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Psuper
Psilocybin


Registered: 02/12/08
Posts: 2,874
Loc: PNW
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Re: how to inoculate agar dishes [Re: Sivarted]
#25294795 - 06/27/18 11:21 AM (5 years, 7 months ago) |
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Quote:
Sivarted said:
Quote:
Jakeoncid419 said: But I can’t start my own posts yet so if there are no recent relevant threads idk what to do and I can’t make new posts until I make so many posts lol it’s a paradox
There are a ton of threads about premade petris.
Use the search engine.
The fellow is engaging in discussion inside of a relevant thread. Telling folks offhandedly to simply use the search engine doesn't contribute anything helpful.
Do you think criticizing this person, instead of helping them along a journey to becoming an ally of the psychedelic mushroom, is actually helpful? This is a serious question for you, Sivarted.
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: how to inoculate agar dishes [Re: Psuper]
#25294799 - 06/27/18 11:22 AM (5 years, 7 months ago) |
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Actually rule #1 is search easy stuff first. Teach a man to fish.
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Psuper
Psilocybin


Registered: 02/12/08
Posts: 2,874
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Re: how to inoculate agar dishes [Re: Psuper] 1
#25294822 - 06/27/18 11:31 AM (5 years, 7 months ago) |
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Jakeoncid419, There are many alternative, and much more friendlier personalities inside our forums. Welcome to the community, and I am sorry you didn't get a warmer welcome.
Folks should understand that those with access to psilocybin, or just access to any positive psychedelic experience, are part of a small and lucky minority of living humans.
I feel such genuine gratitude towards psilocybe mushrooms and this community as a whole, that the narcissism and negative critiquing really puts me off.
If you need a break from the rude and unnecessary tone of some our forum members, feel free to PM me if there is perhaps something I could help you discover.
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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Psuper
Psilocybin


Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 4 months
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1..2..3.. [Re: Psuper]
#25294829 - 06/27/18 11:33 AM (5 years, 7 months ago) |
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-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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Sivarted
Stranger
Registered: 08/22/15
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Re: how to inoculate agar dishes [Re: Psuper]
#25294961 - 06/27/18 12:53 PM (5 years, 7 months ago) |
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Quote:
Psuper said: Quote:
Sivarted said:
Quote:
Jakeoncid419 said: But I can’t start my own posts yet so if there are no recent relevant threads idk what to do and I can’t make new posts until I make so many posts lol it’s a paradox
There are a ton of threads about premade petris.
Use the search engine.
The fellow is engaging in discussion inside of a relevant thread. Telling folks offhandedly to simply use the search engine doesn't contribute anything helpful.
Do you think criticizing this person, instead of helping them along a journey to becoming an ally of the psychedelic mushroom, is actually helpful? This is a serious question for you, Sivarted.
Heres my serious answer.
He zombied a years old thread to ask a very general question about prepared petris.
The thread wasn't about pre-prepared petris, so no, it wasnt a relevant thread.
Furthermore, there are plenty of actual threads about that topic (I know, because I searched, and theres some good info out there).
Not trying to be a dick, but all three of those things I just said are true.
So...
Use the search engine.
And yes, I believe that is helpful. In fact, there is *so much* info in these forums already, it's probably the most helpful thing you could possibly say to anybody with very general questions, like "is MEA good?"
Edited by Sivarted (06/27/18 12:55 PM)
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Jakeoncid419
Follow me.....


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Re: how to inoculate agar dishes [Re: Sivarted]
#25295035 - 06/27/18 01:42 PM (5 years, 7 months ago) |
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Psuper thank you I’ll message u in a bit. Really that’s what I’m looking for sum live dialogue with ppl who know about more than just growing cubes. I only just now created an account here because of all the hostility I read in the forums the Facebook groups are much friendlier but not nearly as much knowledge unless you’re just trying to grow cubes. Using online forums has never been my forte unless it’s on a PS4 or how to operate evolution when it was still up and some simple PGP encryption I’m pretty computer stupid definitely forum stupid. I’ve never been in a form like this computers just never been my thing plus I’m doing this all on my smart phone as I don’t even own an actual computer so reading through post after post gets tiring. Anyway I’m not stressing it. the tree that sways in the breeze doesn’t snap in the storm
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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pixelpopper
Crap Artist

Registered: 09/20/13
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Re: how to inoculate agar dishes [Re: Jakeoncid419]
#25295074 - 06/27/18 02:05 PM (5 years, 7 months ago) |
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The fact that any user can create an account and ask the most skilled growers any question they want is pretty incredible. Equally incredible is the level of knowledge available here to anyone willing to look.
Treat this opportunity with the respect it deserves.
Sometimes people can be grumpy, well its easy with the volume of posts they are responding to over the years, always the same things over and over.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#25295167 - 06/27/18 02:05 PM (5 years, 7 months ago) |
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This thread has been closed.
Reason: This is a forum where adults come to grow drugs. For anyone to cry narcissism is as ridiculous as it gets, this place is built of contribution that no one is paid for. Some of us have practically wrote a books on cubensis cultivation for free and published it here for all.
Like I said our first rule in this fourm is use the search engine. While it's not the most helpful response, sometimes it's the most appropriate.
There's people who's first post is a grow after doing research. There's some people who don't know how to make a post. Some people don't like to read the rules, or look around first. Or don't have the same aptitude. No ones being harsh or mean, and if they are the mods will take care of it.
We all bend over backwards to help people grow shrooms. And sometimes that means the best advice is the advice you don't want to hear. While utse is a usually poor response so is complaining about it. Ever had a job before? Someone is going to tell you to just fucking figure it out, and everyone here is nicer than that.
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