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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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how to inoculate agar dishes
#21916393 - 07/08/15 07:00 PM (8 years, 6 months ago) |
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Hello... Quick question...I followed pasty's agar tek, and have the pda jars cooling right now in the pc...my question is, I didn't c what the best way to inoculate the plates would b?
I have prints, and one homemade spore syringe. Can I just simply scrape a few spores in there and close it up?
Thank you all so much! I really do LOVE this community
Ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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DensePlacebo
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21916404 - 07/08/15 07:02 PM (8 years, 6 months ago) |
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Yup. It's best done ina GB or SAB. But you can use the syringe or a little scraping of a print. I do believe the syringe is the better way to go tho, you spread less spores out which is what you want.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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mushpunx
Fungus Punk



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Re: how to inoculate agar dishes [Re: DensePlacebo] 1
#21916513 - 07/08/15 07:23 PM (8 years, 6 months ago) |
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A drop from a spore syringe will spread around on the agar and it can kind of make it hard to pick out clean/healthy growth sometimes.
If you use the syringe squirt a drop on a loop or a sterile cotton swab first.
To inoculate from the print, flame your loop, cool in a blank plate and swipe the print.. the agar will help pick up spores... then swipe the reciving plate. Swiping is good, youll have an idea of where to expect germination
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 Amateur Mycologists United AMU Q&A
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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Re: how to inoculate agar dishes [Re: mushpunx]
#21916580 - 07/08/15 07:35 PM (8 years, 6 months ago) |
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Thank you guys so much...what exactly does "cool in a blank plate" mean?
Thank you, ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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SizlChest
Padawan



Registered: 09/29/13
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Re: how to inoculate agar dishes [Re: Ganjaburger] 1
#21916619 - 07/08/15 07:47 PM (8 years, 6 months ago) |
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Means after flaming the loop, plunge the hot metal into a section of agar off to the side of the plate. This cools the tool so you can use it without damaging the spores. It also leaves melted agar on the loop, so the spores stick to the agar coating it.
Buy RR's Let's Grow Mushrooms videos. Very good investment to make, I still watch them from time to time.
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
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Re: how to inoculate agar dishes [Re: SizlChest]
#21916781 - 07/08/15 08:21 PM (8 years, 6 months ago) |
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Yes... the videos are definitely a good investment. They are a great reference tool. Though I have been into the hobby for a little while, I watch a vid(s) to freshen up or look up some info quite often.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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Alright, so I made the pda mixture, pc'd, then let cool overnight...when I got them out, the bottom half solidified, top half was still liquid...what gives..I measured out the formula Damn near to the mg on my digital scale. Cooked on low. Added food color, also I can see that any sediment (I assume potato flakes that didn't fully dissolve) where all contained in the solid(bottom) section. I put them in fridge, and they are solidified all the way though, are they still good to use? Even tho I can c that all the sediment is in the bottom half, top half completely clear, besides food coloring.
Ty, I understand there is allot of questions, and I really appreciate the help!!!! :-)
Ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21919979 - 07/09/15 01:14 PM (8 years, 6 months ago) |
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Sediment is fine. Mine look that way when I use instant potato flakes.
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SublimeLBC
dab master



Registered: 10/22/04
Posts: 618
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Re: how to inoculate agar dishes [Re: Grey]
#21919991 - 07/09/15 01:22 PM (8 years, 6 months ago) |
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--------------------
Edited by SublimeLBC (07/09/15 01:23 PM)
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Mr.GuessWork
Stranger

Registered: 03/30/13
Posts: 4,563
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Re: how to inoculate agar dishes [Re: Grey]
#21920011 - 07/09/15 01:28 PM (8 years, 6 months ago) |
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You're trying to isolate mycelium I take it? You want to do what's called a streak plate. The basic idea is to spread everything out on the surface of the agar so individual colony forming organisms are far apart before they start growing. That way when stuff has grown for a bit it won't all be jumbled together, and you can cut the sections you want out of the agar. Here's a video for the 4 streak method:
You probably don't need to do 4 streaks (2 or three might work fine). Make sure you re-sterilize your loop after each streak.
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DensePlacebo
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Re: how to inoculate agar dishes [Re: Mr.GuessWork]
#21920879 - 07/09/15 05:04 PM (8 years, 6 months ago) |
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IME pastys agar tek gave me realm watery agar. I still use that tek to this day but I use double the agar he calls for. I don't have sediment and I don't have lots of condensation.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 8 hours
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Re: how to inoculate agar dishes [Re: DensePlacebo]
#21921551 - 07/09/15 07:44 PM (8 years, 6 months ago) |
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If you put a folded piece of paper towel between the lid and foil it helps alleviate condensation and/or steam getting into the container.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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20g/L of agar-agar should give you appropriate consitency. 15g/L is most lab grade. if you buy agar from the store make sure it doesn't have added ingredients or sugar otherwise you're not adding as much as you think you are.
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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Re: how to inoculate agar dishes [Re: bodhisatta]
#21922155 - 07/09/15 09:40 PM (8 years, 6 months ago) |
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Thank u guys so much for all the speedy answers...Ok..so feel like the more I learn, the more questions I think of..lol. but I have a pe swab...do I just streak it the same way I would if using an inoculation loop? Also, I've only worked with grain in all of my past grows... Recently I had a bunch of brf/verm given to me...I was obviously familiar with pf-tek...but never tried it...when I went to look up ratios of Verm/brf/h2o....I read a few different answers...so, what ratio do u guys use? I really want to try and "perfect"every technique...and recently ran into some extra funds so I can buy whatever is needed. Also, how long do ur guys brf jars take to show initial growth? I'm on day 4/5 with no noticeable growth...starting to get worried..sorry for the long one paragraph form..lol. 2mg clonazolam, &2mg alprazolam, and a couple beers..my grammar/writing skills go right out the window...lol
Thank you guys so much for all the help....I don't understand how Fuckin Facebook and instagram are so popular...the shroomery is a million x cooler
-Ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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DensePlacebo
Stranger



Registered: 05/14/15
Posts: 186
Loc: canada
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21922388 - 07/09/15 10:20 PM (8 years, 6 months ago) |
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I believe the streak is the same but wait for one of them others to clear that up. I use 1 part water 1 part flour mix that really good and add 2 parts verm I also add in a pinch or two of gypsum but there are a few different additives. I see growth in about a week so id say your fine still.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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Ganjaburger
mushroom aficionado


Registered: 07/30/09
Posts: 710
Loc: Midwest
Last seen: 6 years, 3 months
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Re: how to inoculate agar dishes [Re: DensePlacebo]
#21922471 - 07/09/15 10:52 PM (8 years, 6 months ago) |
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I've heard of adding gypsum...what is it, and y add it?
Thx ganjaburger
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Trade /sell list...plenty of cube prints....microscope, two hundred dollar brand new Aerobed, and a ti-84+ calculator....
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,064
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#21922565 - 07/09/15 11:21 PM (8 years, 6 months ago) |
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Quote:
Ganjaburger said: Hello... Quick question...I followed pasty's agar tek, and have the pda jars cooling right now in the pc...my question is, I didn't c what the best way to inoculate the plates would b?
I have prints, and one homemade spore syringe. Can I just simply scrape a few spores in there and close it up?
Thank you all so much! I really do LOVE this community
Ganjaburger
Use your own prints. Read the tiger drop tek in my signature. If you use pre-sterilized cotton swabs you won't even need to flame. If the print was taken with any sort of care you should be able to get clean growth on your initial inoculation and then you can drop the whole agar plate into a grain jar.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Jakeoncid419
Follow me.....


Registered: 06/26/18
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Re: how to inoculate agar dishes [Re: Ganjaburger]
#25293010 - 06/26/18 12:31 PM (5 years, 7 months ago) |
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WhAt do u think of pre fabed agar dishes? Recommend vendor? I need to isolate cope stain for grain inoculation. Still rather new to agar and never made my own
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: how to inoculate agar dishes [Re: Jakeoncid419]
#25293104 - 06/26/18 01:45 PM (5 years, 7 months ago) |
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Premade plates are shit. Make your own plates. Also this is a two year old thread.
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Jakeoncid419
Follow me.....


Registered: 06/26/18
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Re: how to inoculate agar dishes [Re: bodhisatta]
#25294489 - 06/27/18 09:01 AM (5 years, 7 months ago) |
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Oh I didn’t even look at dates, does it matter that it’s a old thread? Sry for my ignorance new member although I’ve been just reading for years lol malt extract good route for copes ya think?
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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