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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Contamination??
    #21914580 - 07/08/15 11:36 AM (8 years, 6 months ago)

Well my jar showing the most growth isn't like solid white, it's growing this greyish white stuff. I tried to take a pic, but it didn't do it justice so fuck it. Now I found my other jar which had pristine white mycelium growing now has what appears to be a tiny green spot next to the mycelium. And one of the 8 jars that showed no growth now has a green spot on it as well. Going to throw out the jar that has green and no mycelium, but I'm gunna keep an eye on the one that has mycelium to make sure I'm not just seeing things.

So my question is whether or not mycelium can grow in a greyish-white color? because the jar that has this greyish-white color growth is looking like the only viable cake I have colonizing. 7 out of 10 showed no growth so far and 2 showed green growth.


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OfflineBeethoven1770
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Registered: 05/17/15
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Re: Contamination?? [Re: Dr.Satan]
    #21914605 - 07/08/15 11:44 AM (8 years, 6 months ago)

Nope, myc is always pure white. Gray-white sounds like cobweb. Green isn't good either. Sorry :frown:


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Contamination?? [Re: Beethoven1770]
    #21914674 - 07/08/15 12:01 PM (8 years, 6 months ago)

Ehh no worries, back to the drawing board :P


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Contamination?? [Re: Dr.Satan]
    #21915152 - 07/08/15 01:49 PM (8 years, 6 months ago)

Okay so the 2 jars with green looking stuff inside were definite contams I opened the one with mycelium and planted that bitch with some cow manure outside, but the contam growing on these 2 was a weird color light green with a hint of blue. Any thoughts on what it was?.

The one with the greyish white I'm just going to leave unopened for now.

The other 7 from that batch I'm going to keep around until the 14 day mark, but I'm expecting them to either grow contams or not grow anything.

I have a single jar inoc'ed with the last cc of the same solution as the 10 previous, using better air-tight sterilization incase moisture leaking into the jars during sterilization might of been the issue. It might have better results, but only time will tell.


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Contamination?? [Re: Dr.Satan]
    #21922917 - 07/10/15 01:47 AM (8 years, 6 months ago)

Update:
The remaining jars are showing promise! I suppose I was just being too impatient with them. Let's just hope they don't randomly grow contams like the others lol.


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OfflineMachiavelliavore
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Re: Contamination?? [Re: Beethoven1770]
    #21923033 - 07/10/15 03:32 AM (8 years, 6 months ago)

Quote:

Beethoven1770 said:
Nope, myc is always pure white. Gray-white sounds like cobweb. Green isn't good either. Sorry :frown:




I've found that it can appear grayish just after germination.  This tends to last a day or two at most.  I think it's simply seeing the background through the extremely thin young mycellium.

Usually said young mycellium exhibits orthogonal growth outward from the substrate, like tons of tiny radial spikes, while contam molds have a more chaoatic and thinner look, remain gray, and sort of crawl over the surface.

If you haven't chucked all your contam cakes yet, you can steam them again and reinoculate, and probably have good chances of success.  Up to you if you want to risk your spore solution.  It's bacteria that are hard to kill with heat, not fungi and their spores, afaik.  90 minutes may not even be required for that task.  Molds are just way more aggressive once they get going.

Did it appear the contaminants originated from your spore inoculation?  I had some experimental cakes contaminate from an old spore syringe, so I chucked them in my PC for half an hour at 15psi.  The growth on them appears to be dead and a new incolulation is germinating.  I guess I'll know pretty soon if I really killed it.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleSteveRogers
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Re: Contamination?? [Re: Machiavelliavore]
    #21923237 - 07/10/15 06:11 AM (8 years, 6 months ago)

Quote:

Machiavelliavore said:

If you haven't chucked all your contam cakes yet, you can steam them again and reinoculate, and probably have good chances of success. 






Do Not do This.


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OfflineFreeWorldOrder
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Re: Contamination?? [Re: SteveRogers]
    #21923418 - 07/10/15 07:34 AM (8 years, 6 months ago)

I would just keep an eye on them... I have had greyish mycelium in the past when it first started colonizing. The jars ended up turning out fine.

While getting cobweb is definitely possible , it doesn't seem to be near as prevalent as say, trich. At least IME.... I have only had cobweb one time.

Also, cobweb will usually overtake a jar or sub in just a couple days.


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OfflineTravelAgency
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Registered: 12/25/10
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Re: Contamination?? [Re: SteveRogers]
    #21923765 - 07/10/15 09:24 AM (8 years, 6 months ago)

Quote:

SteveRogers said:
Quote:

Machiavelliavore said:

If you haven't chucked all your contam cakes yet, you can steam them again and reinoculate, and probably have good chances of success. 






Do Not do This.




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InvisibleMad Season
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Registered: 09/16/12
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Re: Contamination?? [Re: TravelAgency]
    #21923923 - 07/10/15 10:22 AM (8 years, 6 months ago)

Quote:

Mad Season said:
I'll have to dig up some pics for yall. I swear I've seen way worse get called mycelium.

http://www.shroomery.org/forums/showflat.php/Number/21757581

turned to:

http://www.shroomery.org/forums/showflat.php/Number/21624840

and this



turned into:



I told both of these people they had bad stuff haha. What op has looks close to or even better than those examples. I bet it's fine :smile:




Proof that grey can indeed be mycelium. These examples are quite extreme. I've never had it be like this before, but you can even have grey spots in fully colonized jars that are still mycelium.


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OfflineMachiavelliavore
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Re: Contamination?? [Re: Mad Season]
    #21924058 - 07/10/15 11:11 AM (8 years, 6 months ago)

Do you know if the mycellium that appears gray is white under a microscrope and simply too fine for the eye to resolve against a dark background, translucent when thin, or if it is truly gray?

BTW OP, since everyone is biting my head off about trying to resterilize a few jars with some mold spots, I suppose I should clarify that I would only try that if I had an abundance of spore solution.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Contamination?? [Re: Machiavelliavore]
    #21924083 - 07/10/15 11:19 AM (8 years, 6 months ago)

Mycelium just comes in different colors lol. I wish I was home but I bet I could find a dark as hell jar and a white one. When 2 spores make a hyphae, the mycelium they make can be greyish. Definitely nowhere near cobweb/dactylium/etc. grey, which is a dark ass grey. Very distinguished. Dark and fluffy or rizmorphic can still grow just as fast and produce just as many pins though.

The only reason I'd be against reusing contaminated jars would be moisture content would be down after a prolonged time, and the contaminations, although maybe nonexistent, would still produce a lower biological efficiency due to already digesting nutrients. Also it would be lower due to having a lower amount of hydration due to a second sterilization run.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/10/15 11:23 AM)


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Contamination?? [Re: SteveRogers]
    #21925472 - 07/10/15 05:26 PM (8 years, 6 months ago)

Quote:

SteveRogers said:
Quote:

Machiavelliavore said:

If you haven't chucked all your contam cakes yet, you can steam them again and reinoculate, and probably have good chances of success. 






Do Not do This.




No worries I didn't plan on it, but glad to see all your replies!

Update:

The grey growth in the one jar has begun to turn yellowish and white mycelium has begun to grow right next to it

The jar I scooped the contam'ed mycelium out of actually was saved when I did this! It's now growing healthy mycelium! Without the dry verm barrier now of course.

I have 5 of the 7 jars that showed no growth, growing healthy mycelium now (slowly but surely)


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