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Peaceful Soul
Noobie



Registered: 04/25/15
Posts: 70
Loc: EU
Last seen: 1 year, 5 months
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OK to PC oat jars with small amount of contamination?
#21914161 - 07/08/15 09:36 AM (8 years, 6 months ago) |
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Hi guys! I need your advice
3 or 4 days ago I prepared some oat spawn. I gave them a 40 min boil and put them in jars but doe to low capacity of my pressure cooker and limited amount of time I did not manage to PC all the jars.
I can see that in the meantime some green contaminant, and some black contaminant has appeared. It´s not a lot, but it is there:


Is it ok to PC jars, and inoculate with LC or Agar? I mean, not much nutrition can be missing do to the contamination
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shroomyaxn



Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
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Re: OK to PC oat jars with small amount of contamination? [Re: Peaceful Soul]
#21914661 - 07/08/15 11:57 AM (8 years, 6 months ago) |
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Throw that grain out, clean the jars with hot soap and water. If you have a dishwasher run the jars through a cycle.
Take the time to prep new spawn. PC 90 minutes at 14-15 psi.
Good luck.
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Peaceful Soul
Noobie



Registered: 04/25/15
Posts: 70
Loc: EU
Last seen: 1 year, 5 months
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Re: OK to PC oat jars with small amount of contamination? [Re: shroomyaxn]
#21914867 - 07/08/15 12:42 PM (8 years, 6 months ago) |
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Quote:
shroomyaxn said: Throw that grain out, clean the jars with hot soap and water. If you have a dishwasher run the jars through a cycle.
Because: A; You think that there is a risk that the contaminant will survive 90min of PC? B; You think that a lot of the oat´s nutritional value is already used by the contaminant? C; You have other reasons for tossing the jars (please explain!)
Quote:
shroomyaxn said:Good luck.
Thx mate
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oregon.trailboy
OTB



Registered: 12/15/14
Posts: 1,641
Loc: Oregon
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Re: OK to PC oat jars with small amount of contamination? [Re: Peaceful Soul]
#21914911 - 07/08/15 12:50 PM (8 years, 6 months ago) |
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Pasty talks of pc'ing for 90 mins @ 15psi and after that if endospores are still visible then pc again for 60-90 mins @ 15psi.
I don't know if pasty seen contams before his first pc run. but I'm sure in your case that if you did one cycle an if the contams ain't dead run again
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: OK to PC oat jars with small amount of contamination? [Re: oregon.trailboy]
#21914960 - 07/08/15 01:05 PM (8 years, 6 months ago) |
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endospores are still visible? Pasty must have good eyes nah but you dont know if they are there unless you notice your BE is hurting with your new batch of grains.
you can re-sterilize the jars yes. 90mins 15 psi kills that green spot like childs play. I'd be more worried about the water content. some grains looks shriveled while others look really wet..
no need to excessively wash jars either, they get sterilized in the pc just like the grains do.
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Peaceful Soul
Noobie



Registered: 04/25/15
Posts: 70
Loc: EU
Last seen: 1 year, 5 months
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Re: OK to PC oat jars with small amount of contamination? [Re: spacechildo]
#21915201 - 07/08/15 02:03 PM (8 years, 6 months ago) |
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Quote:
oregon.trailboy said:but I'm sure in your case that if you did one cycle an if the contams ain't dead run again 
I did not finish the cycle, The Jars have newer bin in the PC But I think I will just PC now and put them to use...
Quote:
spacechildo said: endospores are still visible? Pasty must have good eyes nah but you dont know if they are there unless you notice your BE is hurting with your new batch of grains.
you can re-sterilize the jars yes. 90mins 15 psi kills that green spot like childs play. I'd be more worried about the water content. some grains looks shriveled while others look really wet..
I am pretty sure the water content is right and that the jar will even out in the PC so I will go for the run 
What does BE mean by the way?
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oregon.trailboy
OTB



Registered: 12/15/14
Posts: 1,641
Loc: Oregon
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Re: OK to PC oat jars with small amount of contamination? [Re: spacechildo]
#21915260 - 07/08/15 02:17 PM (8 years, 6 months ago) |
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Quote:
spacechildo said: endospores are still visible? Pasty must have good eyes
He notices something different about it. I'm guessing He was talking of pc'ing his whole oats twice to kill anything that may be present.
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shroomyaxn



Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
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Re: OK to PC oat jars with small amount of contamination? [Re: Peaceful Soul]
#21915423 - 07/08/15 03:02 PM (8 years, 6 months ago) |
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Quote:
Peaceful Soul said: A; You think that there is a risk that the contaminant will survive 90min of PC? B; You think that a lot of the oat´s nutritional value is already used by the contaminant? C; You have other reasons for tossing the jars (please explain!)
I have always learned from reading forums on here to just toss it.
For one thing, after prepping the grains the moisture content is just right. After time the grain will dry out. Spores will germinate best when the moisture content is right, temp is right and the grain is clean. To keep re-using the same nasty jar is something I would not do.
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: OK to PC oat jars with small amount of contamination? [Re: oregon.trailboy]
#21915562 - 07/08/15 03:28 PM (8 years, 6 months ago) |
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Quote:
oregon.trailboy said:
Quote:
spacechildo said: endospores are still visible? Pasty must have good eyes
He notices something different about it. I'm guessing He was talking of pc'ing his whole oats twice to kill anything that may be present.
The reason he did that was because he knew his batch had endospores and standard cycle wasn't doing the trick. So with that bag he ran them twice.
But maybe he'll stop by and say exactly, but that was the gist of it.
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oregon.trailboy
OTB



Registered: 12/15/14
Posts: 1,641
Loc: Oregon
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Re: OK to PC oat jars with small amount of contamination? [Re: d0urd3n]
#21915913 - 07/08/15 04:48 PM (8 years, 6 months ago) |
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Thanks dourden. I was wondering. And hoping he'd pop in as well.
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Alkaloids
3,4,5-trimethoxyphenethylamine



Registered: 11/15/98
Posts: 743
Loc: pubis mons
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Re: OK to PC oat jars with small amount of contamination? [Re: oregon.trailboy]
#21916080 - 07/08/15 05:26 PM (8 years, 6 months ago) |
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If you are seeing visible evidence of contaminants at this stage i would toss those jars out. Now that they have had a few days to sit at probably near ideal temperatures the endemic spore load will be very high.
Sure you could PC them now, but i think your efforts would be better spent preparing new fresh clean grain spawn. You might get rid of most of the spores and live mold/mildew cultures, but you will have to PC them longer and that can cause caramelization of sugars in the grain.
have fun hope things go well
--------------------
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oregon.trailboy
OTB



Registered: 12/15/14
Posts: 1,641
Loc: Oregon
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Re: OK to PC oat jars with small amount of contamination? [Re: Alkaloids]
#21916545 - 07/08/15 07:29 PM (8 years, 6 months ago) |
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Damn alkaloids your an original shroomy member. '98 rockin it old school!
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ShroominMe
Stranger

Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Re: OK to PC oat jars with small amount of contamination? [Re: oregon.trailboy]
#21916581 - 07/08/15 07:35 PM (8 years, 6 months ago) |
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"When in doubt throw it out."
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Alkaloids
3,4,5-trimethoxyphenethylamine



Registered: 11/15/98
Posts: 743
Loc: pubis mons
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Re: OK to PC oat jars with small amount of contamination? [Re: oregon.trailboy]
#21933154 - 07/12/15 11:52 AM (8 years, 6 months ago) |
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Been around a bit. I appreciate the shout outs trailboy.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 6 hours
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Re: OK to PC oat jars with small amount of contamination? [Re: Alkaloids]
#21933440 - 07/12/15 12:58 PM (8 years, 6 months ago) |
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I do a 120 minute PC cycle for whole oats. Especially if I am doing the no-soak, boil method. So far I have had good results. I was getting contams from endo-spores occasionally when only doing a 90 minute cycle.
If I start having problems I am going back to the soak method. But so far things look to be okay. I need to get some more grain jars going to really be able to tell.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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