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Offlineomu_negru
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First time agar, need advice
    #21913680 - 07/08/15 07:01 AM (8 years, 6 months ago)

This is my first time with agar so please be gentle. I inoculated 8 ghetto jars (just put the mixture in and pressure cook them, then added a piece of fruit body from what i considered to be a worthy candidate (earliest fruit from the earliest cluster) to each jar. Tissue was taken from halfway up the stem , inside a still air box. Naturally, since this is my first time, 3 out of 8 jars also got contaminated (2 are not included here due to poor growth of the myc) , but the purpose of this post is to find out what you guys consider to be the best candidate for making a master grain jar. None of them present the rhyzo qualities i've seen in other posts, but using my eye the one with the large contam spot near it looks closest to it (but the size means it's going to grow slower than others). Sorry for the shitty quality in advance but since the tek is ghetto and the jar walls are thick , that's the best i could do.



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OfflineEternalPeace
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Registered: 11/15/14
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Last seen: 7 years, 5 months
Re: First time agar, need advice [Re: omu_negru]
    #21952593 - 07/16/15 03:53 PM (8 years, 6 months ago)

I can't get the images to expand, but from what I can see in the thumbnails, I don't see any contamination, so a tentative grats on that.

Otherwise, I can see some sectors (sections that are different than others), so before you drop some into the master grain, I recommend cleaning it up a bit more.  That way you will be inoculating your master grain with only one culture--the preselected one, which tends to be the fastest growing one, depending on what you select in the agar phase.

Take chunks of the fastest growing, non-contaminated sectors and drop them into a few more rounds of agar.  Continue this for a few weeks, with a new round of transfers each week, or when there has been enough growth to determine if any sectoring is occurring.  Estimate this phase to last at least a month, with a round of transfers per week, before you'll start seeing the uniform growth that you are after.  (I assume YMMV though.)

Check out Stro's awesome guide for more details.  This is what I used on the very first time I ever did it, and it brought me through it perfectly.


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OfflineEternalPeace
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Registered: 11/15/14
Posts: 16
Last seen: 7 years, 5 months
Re: First time agar, need advice [Re: EternalPeace]
    #21957275 - 07/17/15 06:03 PM (8 years, 6 months ago)

Now they are working, so I can see better.  I'd definitely let them grow out more.  That will help show the sectoring more clearly.  You'll see sections that look different than others, and some sections will move faster than others.


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