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OfflineMilaweak
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Registered: 05/29/15
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Salt in Agar
    #21909589 - 07/07/15 12:46 PM (8 years, 6 months ago)

Hi everyone, little question here.

I have access to a culture media powder of this composition :
(it is used for bacterial growth)

    10 g  Peptone 140
    5 g  Yeast Extract
    5 g  Sodium Chloride
    12 g  Agar



Do you know if Myc likes salt ?
I haven't seen it used in agar.

Apparently it wouldn't hurt it :

The use of salt in fighting trichoderma mold

any thoughts ?


Edited by Milaweak (07/07/15 12:47 PM)


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OfflineFreeWorldOrder
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Re: Salt in Agar [Re: Milaweak]
    #21909943 - 07/07/15 02:25 PM (8 years, 6 months ago)

No... you do not want salt. People do try and use it to slow trich but it will damage mycelium. Not sure if new mycelium would even start on agar with salt in it. But either way, no salt.


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Offlinespacechildo
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Re: Salt in Agar [Re: FreeWorldOrder]
    #21909966 - 07/07/15 02:30 PM (8 years, 6 months ago)

your link doesnt match your topic here, dont use salt and dont read scammers' posts :lol: he probably just wanted you to buy some super-salt off him (that you'd never see anyway)


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InvisiblebodhisattaMDiscordReddit
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Re: Salt in Agar [Re: FreeWorldOrder]
    #21909985 - 07/07/15 02:34 PM (8 years, 6 months ago)

mycelium should still grow at that concentration, they use salt agar to make gram negative bacteria suppressed when swabbing a plate with something that would have multiple types of bacteria, and only wanting some of them to grow.

I personally would get a different agar media

salt isn't good for trich treatment, salt is good for inhibiting all growth

trich is a parasite of plenty of other molds than cubensis in nature, but it's still a facultative parasite of cubensis when given opportunity.



this is why you can't cut, salt, etc trich out. salt only helps by being in such high concentrations it inhibits everything. but by the time you see enough trich to grab your salt you're fucked anyway.


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Offlineblackout
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Re: Salt in Agar [Re: bodhisatta]
    #21910072 - 07/07/15 02:57 PM (8 years, 6 months ago)

In Paul Stamets books, "mycelium running" and "growing gourmet and medicinal mushrooms" he speaks of using salt in LCs.

Quote:

the salt limits the bacteria growth without stifling spore germination




Quote:

A gram or two of table salt inhibits bacteria from growing while not substantially affecting the viability of the spores.




I wondered how much it tolerated as I wondered about the possibility of sterilizing with bleach, and letting it degrade into salt and water.
http://www.shroomery.org/forums/showflat.php/Number/4323776


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Offlinespacechildo
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Re: Salt in Agar [Re: blackout]
    #21910084 - 07/07/15 02:59 PM (8 years, 6 months ago)

spore germination is one thing but how about myc growth?


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InvisiblebodhisattaMDiscordReddit
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Re: Salt in Agar [Re: spacechildo]
    #21910093 - 07/07/15 03:01 PM (8 years, 6 months ago)

I bet at the 5-7.5% concentration for salt media it wouldn't hurt mycelium growth all that much.


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OfflineMilaweak
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Re: Salt in Agar [Re: bodhisatta]
    #21913577 - 07/08/15 06:05 AM (8 years, 6 months ago)

I'm eventually going to try it maybe next month.

I'm not going to say that I'll post the results because every time a post ends with OP saying "I'll post my findings", usually it's the last post.  :biggrin:


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Invisibleblindingleaf
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Re: Salt in Agar [Re: Milaweak]
    #21913739 - 07/08/15 07:20 AM (8 years, 6 months ago)

I've used chicken and beef broth agar and v-8 juice agar, and there is salt in that.  i get the low sodium one though.  not sure how many grams of salt in each.


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Re: Salt in Agar [Re: blindingleaf]
    #21913975 - 07/08/15 08:47 AM (8 years, 6 months ago)

Quote:

I have access to a culture media powder of this composition :
(it is used for bacterial growth)

    10 g  Peptone 140
    5 g  Yeast Extract
    5 g  Sodium Chloride
    12 g  Agar





Ah good old LB. It can grow fungi, sort of, but usually slowly and not particularly happily. Works great for E. coli though. I use V8 agar pretty often in lab for plant pathogens, but its not the best for everything.

For general saprobes, MEA and PDA tend to be my go-to choices, both of which are fairly easy to make. Hit up a home brewing store and spend a couple bucks on some light malt extract, or buy a bunch of potatoes and some cheese cloth.


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InvisiblebodhisattaMDiscordReddit
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Re: Salt in Agar [Re: matsc]
    #21914597 - 07/08/15 11:41 AM (8 years, 6 months ago)

Bacon is only 2-4% salt. Vegimite is 7-10% so if that outs perspective on how salty the agar is


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OfflineMilaweak
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Re: Salt in Agar [Re: matsc]
    #21914611 - 07/08/15 11:46 AM (8 years, 6 months ago)

Thank you matsc!

So it's not worth it.. I want Myc to be happy! :tongue2:


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Offlinematsc
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Re: Salt in Agar [Re: Milaweak]
    #21914670 - 07/08/15 12:00 PM (8 years, 6 months ago)

Yeah, LB was invented to study a very specific phenomenon (viral reproduction in E. coli), in organisms which live in a very specific environment (human colon).

So, its high in proteins (though not all are directly useable), relatively high in salt, and very very low in carbohydrates. This is good for studying lysogeny since the E. coli will grow to a nice density for phage behavior, at a rate that is neither too fast or too slow to be useful. Its also dirt cheap to make and since it popped up in some early important papers, everyone else started using it. http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html

Fungi, at least saprobic fungi, tend to prefer a much higher carbohydrate load, usually in the form of complex carbs (starch in PDA, maltose in MEA, etc). They can handle simple sugars fairly well though, within reasonable concentrations, but their growth habit can get a little goofy at times. Salt tolerance is variable, but most that your average hobbyist will deal with can handle it ok. I wouldn't use sea water for my agar or anything, but they wont shrivel up and pickle at the sight of a grain of salt.


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