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Charleskun
Sir Charlington



Registered: 02/25/15
Posts: 207
Last seen: 2 years, 11 months
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LC to Agar
#21909103 - 07/07/15 10:28 AM (8 years, 6 months ago) |
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Hello fellow shroomerites, this is my first time using agar. I inoculated with b lc about 5 days ago. Is this good looking growth so far?
-------------------- All I see Is darkness
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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It doesn't look contaminated to me, but is a poorly made plate. Looks like you squirted way too much LC on to it, and the liquid ran around the sides. You want to keep growth away from the side of the plates. I flame my needle and while still hot poke it in the middle of an agar plate, this makes an indent or little well, I then put a drop or 2 of LC in this well and it will then grow outwards.
posted this before about syringe control
My method of fine syringe control
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blackout said:
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third3y3 said: It's SOOO easy to squeeze too much. I guess I'll get better. Typical newbie type mistake.
Theres a trick to stop this, do not use the syringe like people typically do. I am right handed, I grasp the syringe in my right hand making a thumbs up sign, the plunger end is sticking up with my thumb under the bit you usually press down with your finger. So if I gave a thumbs up out straight the needle is facing the floor.
My right pinky finger is gripping the body of the syringe at the 10ml sign
 Now you can press down the plunger with your left hand while actively pressing upwards with your right thumb. This gives you good control. If a syringe is unused for a while the rubber seal will be subject to static fritcion (aka stiction). If you use a syringe in a normal manner the moment this stiction is overcome the plunger will tend to move far too much due to the force you are applying and the fact nothing is stopping the syringe from going all the way down.
You can do it with just 1 hand too, just practice with water.
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blackout said: I didn't state the obvious, but as the syringe empties obviously my pinky finger moves down the body with the print on it. You can get decent control with just 1 hand too, I usually only use the 2 hand method with a syringe which has not been used in a long time. With the 1 hand method I clench my fist around the syringe. When full my little finger wrapped around the syringe body (with the print) and my other 3 fingers grasp the plunger shaft firmly and my thumb presses down. So the plunger shaft is stopped from moving quickly by the friction of my fingers. As the liquid gets used up I put the next finger on the printed body. For the last 2ml my forefinger is just pressed against the underside of the plunger shaft which again just stops it being able to jump down too much.
Another 1 handed method is having your 3 fingers grasp the body with the printing, and your forefinger wrapped around the plunger shaft which provides resistance. Then press down with your thumb in a controlled manner. As said, just practice with plain water and see what suits you best.
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Charleskun
Sir Charlington



Registered: 02/25/15
Posts: 207
Last seen: 2 years, 11 months
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Yeah I noticed it ran through out the plate when I inoculated, How much should I squirt next time? I only did 2 drops.
-------------------- All I see Is darkness
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Just 1 or 2 drops if you can, and do not let it swirl around. With LC you could inject clear liquid and have no growth. To concentrate my LCs I suck up the LC and let the syringe rest for a few hours or a day. I rest it with the needle in the air. This makes all the myc settle to the bottom. I then keep it held upright and inject the clear upper layer out over a basin. I do not worry about it going on my hands or down the side of the syringe, as I will be flaming it later.
Now you have 1-2ml of concentrated myc and can definitely get away with just 1 drop. If the needle is hot it can kill the myc in that first drop. The needle can be cooled by dipping it in the agar, and this also makes a well or trough like I mentioned, where your drop can go into and not run away.
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Charleskun
Sir Charlington



Registered: 02/25/15
Posts: 207
Last seen: 2 years, 11 months
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Ok cool, thank you for the feedback. I didnt really get a good rundown on the inoculation with an lc so I just winged it. Now I know what to do and what not to do, thanks!
-------------------- All I see Is darkness
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TravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
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Nice blackout! Thanks!
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